Abstract:
:Dynamic visualization of the intravascular events leading to the extravasation of leukocytes into tissues by intravital microscopy has significantly expanded our understanding of the underlying molecular processes. In contrast, the detailed observation of leukocyte transendothelial and interstitial migration in vivo has been hampered by the poor image contrast of cells within turbid media that is obtainable by conventional brightfield microscopy. Here we present a microscopic method, termed reflected light oblique transillumination microscopy, that makes use of the optical interference phenomena generated by oblique transillumination to visualize subtle gradients of refractive indices within tissues for enhanced image contrast. Using the mouse cremaster muscle, we demonstrate that this technique makes possible the reliable quantification of extravasated leukocytes as well as the characterization of morphological phenomena of leukocyte transendothelial and interstitial migration.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Mempel TR,Moser C,Hutter J,Kuebler WM,Krombach Fdoi
10.1159/000073902keywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
435-41issue
5eissn
1018-1172issn
1423-0135pii
73902journal_volume
40pub_type
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