Abstract:
:In this study we aimed to investigate whether the therapeutic efficacy of anisodamine in the treatment of bacteraemic shock could--at least in part--be brought about by its direct interference with the lipopolysaccharide (LPS)-induced activation of endothelial cells. Thus, we investigated the effect of anisodamine on LPS-induced expression of plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF), two major markers of endothelial activation. PAI-1 was measured in the conditioned media of human umbilical vein endothelial cells (HUVEC) by a specific enzyme-linked immunosorbent assay (ELISA) whereas TF activity was measured in the lysates of these cells by using a single step clotting assay. Results obtained in these assays were confirmed on the level of specific mRNA expression by Northern blotting using specific probes for human PAI-1 or TF. In order to evaluate a possible contribution of the NF-kappa B pathway on the effects observed, electrophoretic mobility shift assays (EMSA) were performed using nuclear extracts from HUVEC and NF-kappa B-binding oligonucleotides. When HUVEC were treated with 1 microg/ml LPS a significant increase in PAI-1 and TF activity was observed compared with cells incubated without LPS. Anisodamine dose-dependently inhibited this LPS-induced upregulation of PAI-1 and TF. Anisodamine alone had no effect on the constitutive expression of PAI-1 and TF in these cells. These effects were also confirmed on the level of specific PAI-1 and TF mRNA expression by Northern blotting. Furthermore, we could show by EMSA that anisodamine completely abolished LPS-induced NF-kappa B DNA binding activity in nuclear extracts from HUVEC treated with LPS together with anisodamine. Thus, we provide evidence that anisodamine counteracts endothelial cell activation by inhibiting LPS-induced PAI-1 and TF expression in these cells. Its interference with the NF-kappa B pathway might - at least in part - contribute to this effect. The ability of anisodamine to counteract LPS effects on endothelial cells might be one underlying mechanism explaining its efficacy in the treatment of bacteraemic shock.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Ruan QR,Zhang WJ,Hufnagl P,Kaun C,Binder BR,Wojta Jdoi
10.1159/000051025keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
13-9issue
1eissn
1018-1172issn
1423-0135pii
51025journal_volume
38pub_type
杂志文章abstract::Mechanical forces generated at the endothelium by fluid shear stress and pulsatile stretch are important in ensuring the continuous release of vasoactive endothelial autacoids. Although the mechanism by which endothelial cells are able to detect and convert these physical stimuli into chemical signals is unclear, this...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000025568
更新日期:1998-03-01 00:00:00
abstract::We investigated the possible interference of smooth muscle cells with monocyte response to LDL as well as with their adhesion and transmigration in a coculture of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to e...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000051081
更新日期:2001-09-01 00:00:00
abstract::There is evidence suggesting that fluid shear stress activates phospholipid turnover in endothelial cells, but it is not clear which phospholipids are involved in the transduction of the flow signal. Cultured human umbilical-vein endothelial cells were prelabeled with [14C]-arachidonic acid and subjected to laminar sh...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158963
更新日期:1992-11-01 00:00:00
abstract::Endothelin-1 (ET-1), a vasoconstrictor peptide produced by endothelial and vascular smooth muscle cells (VSMC) might play a role in vascular remodelling. To investigate the proposed 'mitogenic' potential of ET-1, we examined the effects of chronic exposure of VSMC to ET-1 on cell cycle, growth/proliferation and differ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158994
更新日期:1993-07-01 00:00:00
abstract::The vascular endothelium is the source of a number of vasodilator and vasoconstrictor autacoids and is thus a key regulator of vascular homeostasis. We studied the effects of altering the balance between protein tyrosine kinase and phosphatase activity on Ca2+ signalling and phosphotyrosine levels in cultured human en...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159150
更新日期:1996-05-01 00:00:00
abstract::The proinflammatory cytokine interleukin (IL)-1beta and the IL-1 receptor antagonist are expressed by atherosclerotic plaques and may be linked to the development of atherosclerosis. Existing evidence shows that retinoids and their receptors are involved in inflammatory response and that they are found in atherosclero...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000094258
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:Increased peripheral arterial resistances and decreased maximum vasodilation are characteristic features of chronic hypertension. However, little data are available in the literature regarding the possible alterations in the temporal patterns of vasodilatory responses elicited by various stimuli. DESIGN:This...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000090944
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Elevated plasma homocysteine (Hcy) is a risk factor for coronary disease. The objective of this study was to investigate whether Hcy either alone or in high glucose conditions induces endothelin-1 (ET-1) synthesis via the production of reactive oxygen species (ROS). METHODS:Bovine aortic endothelial cells w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000090947
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND AND AIMS:Vasomotion consists in cyclic oscillations of the arterial diameter induced by the synchronized activity of the smooth muscle cells. So far, contradictory results have emerged in the literature about the role of the endothelium in the onset and maintenance of vasomotion. Here our aim is to understan...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000124283
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVE:It is well known that inflammation plays key roles in the development of atherosclerosis and that the transplantation of bone marrow mononuclear cells (BMMNCs) can suppress inflammation in rodent models of ischemic diseases. Here, we explored whether transplantation of autologous BMMNCs could prevent the prog...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449201
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smoot...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000318777
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:Exercise elicits early adaptation of coronary vessels enabling the coronary circulation to respond adequately to higher flow demands. We hypothesized that short-term daily exercise induces biomechanical and functional remodeling of the coronary resistance arteries related to pressure. METHODS:Male rats were su...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000486571
更新日期:2018-01-01 00:00:00
abstract:AIM:Vascular remodeling following injury substantially accounts for restenosis and adverse clinical outcomes. In this study, we investigated the role of the giant scaffold protein Ahnak1 in vascular healing after endothelial denudation of the murine femoral artery. METHODS:The spatiotemporal expression pattern of Ahna...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000464287
更新日期:2017-01-01 00:00:00
abstract::In response to stimuli, endothelial cells release arachidonic acid, a lipid precursor of various vasoactive substances. We have investigated the relationships between cytosolic Ca2+ movements and arachidonic acid release in human umbilical vein endothelial cells. Histamine, a receptor-dependent agonist, and thapsigarg...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025647
更新日期:1999-05-01 00:00:00
abstract::The aim of this study was to use periarterial manipulation to produce an atheroma-like neo-intima in rabbits and study resting blood flow and vascular responsiveness in vivo. One common carotid artery was enclosed in a silastic collar to induce a neo-intima similar to that of human early atherosclerosis, and carotid b...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159043
更新日期:1994-07-01 00:00:00
abstract::The localization of dopamine receptor subtypes in the rabbit pulmonary circulation was investigated by using combined radioligand binding and light microscope autoradiography techniques. Dopamine D1-like receptors, probably belonging to the dopamine D1 receptor subtype, were demonstrated in sections of rabbit lung usi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159094
更新日期:1995-05-01 00:00:00
abstract::Although most physiologically important processes are regulated through negative feedback loops and numerous factors regulating endothelial apoptosis are identified, little is known about negative feedback mechanisms for endothelial apoptosis. Here we describe the release of soluble anti-apoptotic activity by endothel...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000111072
更新日期:2008-01-01 00:00:00
abstract::We evaluated the effects of a simulated workday of prolonged sitting on blood pressure (BP) and pulse wave velocity (PWV) and examined whether posture (seated vs. supine) affected responses. Participants (n = 25) were adults, with overweight/obesity and elevated BP, and performed seated desk work for 7.5 h. BP and PWV...
journal_title:Journal of vascular research
pub_type: 杂志文章,随机对照试验
doi:10.1159/000510294
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND/AIMS:The endothelium has been recognized as a key component in the regulation of blood vessels. We designed a simple procedure to separate endothelial and smooth muscle RNA from rat aorta and mesenteric artery and used this method to establish the distribution of Na(+)/K(+)-ATPase alpha-subunit isoforms (NaK...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000095963
更新日期:2006-01-01 00:00:00
abstract::It has been well established that wall shear stress is an important determinant of endothelial cell function and gene expression as well as of its structure. There is increasing evidence that low wall shear stress, as present in artery bifurcations opposite to the flow divider where atherosclerotic lesions preferentia...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000091648
更新日期:2006-01-01 00:00:00
abstract::Previous studies have demonstrated endothelial and smooth muscle hyperplasia occur during arterial luminal expansion associated with elevation of arterial wall shear rates. The current study investigated whether remodeling induced by elevated wall shear would ultimately result in a vessel with intimal and medial cell ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025592
更新日期:1998-07-01 00:00:00
abstract::The Cytoscan Model E-II (Cytometrics Inc., Philadelphia, Pa., USA) is a newly developed instrument which functions as an intravital microscope and is small and easily portable. Through the use of orthogonal polarization spectral (OPS) imaging, the Cytoscan Model E-II delivers images of the microcirculation which are c...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000054079
更新日期:2000-11-01 00:00:00
abstract::Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159106
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:We have developed an in vitro model by organ culture of rat mesenteric arteries to imitate vascular smooth muscle cell (VSMC) receptor changes in cardiovascular disease. By using this model, alteration of VSMC thromboxane A(2) (TP) receptors was studied. METHODS AND RESULTS:After organ culture of the arteri...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000153247
更新日期:2009-01-01 00:00:00
abstract::The purpose of the present study was to develop a rapid, reproducible method of nonviral gene transfer to the intact vasculature. Male Sprague-Dawley rats were anesthetized, a midline abdominal incision was made and segmental branches of the superior mesenteric artery were dissected free of surrounding mesentery. A sp...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025753
更新日期:2000-09-01 00:00:00
abstract::The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn's disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from prim...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000076126
更新日期:2004-01-01 00:00:00
abstract::This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4 degrees C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2 alp...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159097
更新日期:1995-07-01 00:00:00
abstract::This work compares the expression of adrenoceptors (ARs) and G-protein-coupled receptor kinase (GRK) 2 (RT-PCR and immunoblotting) and functional responses in conductance (aorta) and resistance vessels (mesenteric resistance arteries; MRA) in two different models of rat hypertension: hypertension induced by chronic tr...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000360400
更新日期:2014-01-01 00:00:00
abstract::Vascular restenosis is one of the major concerns for the management of coronary artery disease using therapeutic vascular procedures. Treatments with thrombin-specific inhibitors, hirudin or hirulog-1, reduced ischemic events in coronary artery disease patients. Early started and prolonged infusions of these thrombin ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000051041
更新日期:2001-03-01 00:00:00
abstract::The purpose of this study was to determine the contribution of long-chain fatty acids relative to other mitochondrial substrates in active vascular smooth muscle (VSM). Hog carotid arteries were isometrically contracted in physiological saline solution containing 0.71 mM U-(13)C-oleic acid (bound to albumin at a ratio...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000051056
更新日期:2001-05-01 00:00:00