Abstract:
:Coronary bypass vessels, saphenous vein (SV) and internal thoracic artery (ITA), differ in susceptibility to atherosclerosis and medium- to long-term patency. Whereas most ITA remain patent (90% at 10 years), 20% of SV grafts fail in the first year and approximately 45% fail within 10 years. Reasons for these differences are not fully understood. Loss of SV patency may reflect early metabolic events, particularly increased proteoglycan (PG) synthesis which contributes to intimal volume and promotes atherogenesis through retention of atherogenic lipoproteins. We determined, in vitro, the PG metabolic activity of SV, ITA, and human coronary arteries through autoradiographic detection of incorporated [3H]glucosamine. SV had significantly higher levels of PG synthesis than ITA, especially in the subendothelial zone and after time (7 days) in culture. Patterns of synthesis in coronary vessels were similar to SV with high levels of incorporation in the subendothelial zone of thickened intima (> 100 microm). Increased subendothelial labelling in SV was due to increased PG synthesis, not decreased degradation. ITA showed no propensity for upregulation of subendothelial PG synthesis. Immunohistochemistry showed TGF-beta1 and TGF-beta2 localised primarily to the subendothelial zone of SV and coronary arteries. With time in culture immunostaining increased in parallel with increased PG synthesis. Subendothelial TGF-beta1 and TGF-beta2 were absent in ITA. A panspecific TGF-beta neutralising antibody reduced subendothelial PG synthesis in SV and coronary arteries by 50 and 60%, respectively. These results support the idea that vessels susceptible to atherosclerosis show increased accumulation of subendothelial PG mediated by TGF-beta.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Scott L,Kerr A,Haydock D,Merrilees Mdoi
10.1159/000159245subject
Has Abstractpub_date
1997-09-01 00:00:00pages
365-77issue
5eissn
1018-1172issn
1423-0135journal_volume
34pub_type
杂志文章abstract:BACKGROUND:Lipoxin A4 (LXA4) is a biologically active product generated from arachidonic acid by lipoxygenase action. The production of lipoxins is enhanced by aspirin through acetylation of cyclooxygenase-2, via a mechanism known as 'aspirin-triggered lipoxin'. LXA4 has both anti-inflammatory and proinflammatory actio...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000371490
更新日期:2014-01-01 00:00:00
abstract::Regulation of [Ca2+]i and modulation of the [Ca2+]i sensitivity of myosin phosphorylation in smooth muscles may involve phosphorylation of one or more proteins on tyrosine residues. We tested this hypothesis by measuring tyrosine phosphorylation of proteins extracted from swine carotid artery and separated by SDS gel ...
journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induc...
journal_title:Journal of vascular research
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更新日期:2007-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449202
更新日期:2017-01-01 00:00:00
abstract::It has been well established that wall shear stress is an important determinant of endothelial cell function and gene expression as well as of its structure. There is increasing evidence that low wall shear stress, as present in artery bifurcations opposite to the flow divider where atherosclerotic lesions preferentia...
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pub_type: 杂志文章,评审
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更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:The aim of the present study was to evaluate different signaling pathways by which exercise training would interfere in endothelial function in obesity. Therefore, we examined adipocytokine levels and their receptors in the corpus cavernosum and femoral artery from trained rats on a high-fat diet. METHODS:F...
journal_title:Journal of vascular research
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更新日期:2017-01-01 00:00:00
abstract:AIMS:We investigated the role of superoxide O(2)(-) during the initiation of vascular endothelial growth factor (VEGF)- and placental growth factor (PlGF)-mediated signal transduction in bone marrow-derived endothelial cells. METHODS:BMhTERT cells were treated with VEGF or PlGF in the presence or absence of antioxidan...
journal_title:Journal of vascular research
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journal_title:Journal of vascular research
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更新日期:2007-01-01 00:00:00
abstract::The Cytoscan Model E-II (Cytometrics Inc., Philadelphia, Pa., USA) is a newly developed instrument which functions as an intravital microscope and is small and easily portable. Through the use of orthogonal polarization spectral (OPS) imaging, the Cytoscan Model E-II delivers images of the microcirculation which are c...
journal_title:Journal of vascular research
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159237
更新日期:1997-07-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000025622
更新日期:1999-01-01 00:00:00
abstract::In response to stimuli, endothelial cells release arachidonic acid, a lipid precursor of various vasoactive substances. We have investigated the relationships between cytosolic Ca2+ movements and arachidonic acid release in human umbilical vein endothelial cells. Histamine, a receptor-dependent agonist, and thapsigarg...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025647
更新日期:1999-05-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000235968
更新日期:2010-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000110208
更新日期:2008-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000258905
更新日期:2010-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000461581
更新日期:2017-01-01 00:00:00
abstract::Ruptured atherosclerotic plaques, lined with activated platelets, constitute an attractive target for magnetic resonance imaging (MRI). This study evaluated whether microparticles of iron oxide (MPIO) targeting ligand-induced binding sites (LIBS) on the activated conformation of glycoprotein IIb/IIIa could be used to ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000135660
更新日期:2009-01-01 00:00:00
abstract::Recent research in ultrasound technology has led to the development of a high-resolution echo-tracking device. The present study was performed to evaluate the accuracy in the measurement of human radial artery intima-media thickness with this new device. We determined the correlation between histological and ultrasoni...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159037
更新日期:1994-03-01 00:00:00
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journal_title:Journal of vascular research
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159043
更新日期:1994-07-01 00:00:00
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更新日期:2017-01-01 00:00:00
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journal_title:Journal of vascular research
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更新日期:2000-11-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:1993-07-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:Journal of vascular research
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更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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更新日期:2004-11-01 00:00:00