Abstract：
BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induced angiogenesis is preceded by vascular leak and inflammation, which have been proposed to mediate subsequent angiogenesis. METHODS:To test this hypothesis, we infused VEGF into the brains of adult rats to induce pathological angiogenesis. Some of these rats were treated with dexamethasone, a potent anti-inflammatory glucocorticoid, to inhibit inflammation and edema. RESULTS:We demonstrate that inhibition of inflammation by treatment with dexamethasone significantly attenuated VEGF-induced pathological angiogenesis. To present converging evidence that inflammation may be important in this angiogenic process, we also demonstrate that mice genetically deficient in the inflammatory mediator intercellular adhesion molecule-1 have attenuated VEGF-induced angiogenesis. These same mice showed normal amounts of physiological angiogenesis in response to enriched environments, however, suggesting that a generalized reduction in vascular plasticity could not account for their poor angiogenic response to VEGF. CONCLUSIONS:Taken together, the data from these experiments suggest that the inflammation which occurs before or during VEGF-induced pathological brain angiogenesis plays a contributory role in the pathological angiogenic process.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Kasselman LJ,Kintner J,Sideris A,Pasnikowski E,Krellman JW,Shah S,Rudge JS,Yancopoulos GD,Wiegand SJ,Croll SDdoi
10.1159/000101450subject
Has Abstractpub_date
2007-01-01 00:00:00pages
283-91issue
4eissn
1018-1172issn
1423-0135pii
000101450journal_volume
44pub_type
杂志文章abstract：:The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn's disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from prim...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000076126
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:Angiopoietin-like protein 3 (ANGPTL3) is a liver-derived plasma protein that modulates plasma triglyceride clearance, angiogenesis and atherosclerosis in experimental models. So far, no study has examined its role in atherosclerosis in human subjects. We evaluated the possible association between plasma ANGP...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000098153
更新日期：2007-01-01 00:00:00
abstract：:Endothelin-1 (ET-1), a vasoconstrictor peptide produced by endothelial and vascular smooth muscle cells (VSMC) might play a role in vascular remodelling. To investigate the proposed 'mitogenic' potential of ET-1, we examined the effects of chronic exposure of VSMC to ET-1 on cell cycle, growth/proliferation and differ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158994
更新日期：1993-07-01 00:00:00
abstract：:Actin cytoskeleton reorganization is regulated by various actin-binding proteins. Cofilin is the principal filament-depolymerizing protein, whose activity is reduced upon phosphorylation by LIMK. Thus, LIMK and cofilin comprise a signal transduction module regulating actin turnover and myogenic tone in healthy vascula...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000081247
更新日期：2004-09-01 00:00:00
abstract：PURPOSE:To determine: (1) whether variables of a pulse wave form analysis of ocular microcirculation shown by laser speckle flowgraphy (LSFG) correlate with left ventricular (LV) systolic dysfunction and (2) whether these variables correlate with LV diastolic dysfunction in subjects without LV systolic dysfunction as a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000494066
更新日期：2018-01-01 00:00:00
abstract：:Mechanical forces generated at the endothelium by fluid shear stress and pulsatile stretch are important in ensuring the continuous release of vasoactive endothelial autacoids. Although the mechanism by which endothelial cells are able to detect and convert these physical stimuli into chemical signals is unclear, this...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000025568
更新日期：1998-03-01 00:00:00
abstract：:Regulation of [Ca2+]i and modulation of the [Ca2+]i sensitivity of myosin phosphorylation in smooth muscles may involve phosphorylation of one or more proteins on tyrosine residues. We tested this hypothesis by measuring tyrosine phosphorylation of proteins extracted from swine carotid artery and separated by SDS gel ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159196
更新日期：1997-01-01 00:00:00
abstract：:In the present study, we investigated the actions of calmodulin (CaM) and CaM-dependent protein kinase II (CaMK-II) on the L-type Ca2+ currents (ICa(L)) of cultured vascular smooth muscle (VSM) cells (A7r5 cell line), using the whole-cell voltage clamp method. The peak IBa (Ca2+ channel using 5 mM Ba2+ as charge carri...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025599
更新日期：1998-09-01 00:00:00
abstract：OBJECTIVES:Extracellular nucleotide release at the site of arterial injury mediates the proliferation and migration of vascular smooth muscle cells. Our aim was to investigate the role of the P2Y2 nucleotide receptor (P2Y2R) in neointimal hyperplasia. Approach and Results: Vascular injury was induced by the implantatio...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449059
更新日期：2016-01-01 00:00:00
abstract：:Although most physiologically important processes are regulated through negative feedback loops and numerous factors regulating endothelial apoptosis are identified, little is known about negative feedback mechanisms for endothelial apoptosis. Here we describe the release of soluble anti-apoptotic activity by endothel...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000111072
更新日期：2008-01-01 00:00:00
abstract：:The Notch4 signaling pathway of endothelial progenitor cells (EPCs) may play a crucial role in Kawasaki disease (KD). We investigated the proliferation, adhesion, migration, angiogenesis, and expression levels of Notch4, recombination signal-binding protein-Jκ (RBP-Jκ), P-selectin, and vascular cell adhesion molecule-...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449061
更新日期：2016-01-01 00:00:00
abstract：PURPOSE:Exercise elicits early adaptation of coronary vessels enabling the coronary circulation to respond adequately to higher flow demands. We hypothesized that short-term daily exercise induces biomechanical and functional remodeling of the coronary resistance arteries related to pressure. METHODS:Male rats were su...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000486571
更新日期：2018-01-01 00:00:00
abstract：:The aim of this study was to use periarterial manipulation to produce an atheroma-like neo-intima in rabbits and study resting blood flow and vascular responsiveness in vivo. One common carotid artery was enclosed in a silastic collar to induce a neo-intima similar to that of human early atherosclerosis, and carotid b...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159043
更新日期：1994-07-01 00:00:00
abstract：:Most virus-infected cells release interferon-beta (IFN-beta) as a powerful inducer of antiviral defense. Endothelial cells tightly regulate local immune cell recruitment by expression of adhesion molecules and chemokines. Here, we studied the transcriptional regulation of IFN-beta-induced chemokine expression in prima...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000097977
更新日期：2007-01-01 00:00:00
abstract：:Migration of vascular smooth muscle cells (SMCs) leading to neointimal hyperplasia is an early and cardinal feature of atherogenesis. Migration of rat aortic SMCs from an upper chamber towards a lower one has been studied in a microchemotaxis (Boyden) chamber. Spontaneous migration of SMCs was practically prevented by...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025580
更新日期：1998-05-01 00:00:00
abstract：:It has been well established that wall shear stress is an important determinant of endothelial cell function and gene expression as well as of its structure. There is increasing evidence that low wall shear stress, as present in artery bifurcations opposite to the flow divider where atherosclerotic lesions preferentia...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000091648
更新日期：2006-01-01 00:00:00
abstract：:Pituitary adenylate cyclase-activating peptide (PACAP; 1-38 and 1-27) and vasoactive intestinal peptide (VIP) are related neuropeptides of the secretin/glucagon family. Overlapping signaling through G-protein-coupled receptors mediates their vasomotor activity. We previously showed that PACAP deficiency (PACAP-KO) shi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000481781
更新日期：2017-01-01 00:00:00
abstract：:In coronary artery diseases, cigarette smoking is a risk factor and the endothelin system plays a key role in the pathogenesis. This study was to examine if dimethylsulfoxide-soluble smoke particles (DSP) upregulate endothelin type-B (ETB) receptors in the coronary artery and investigate the mechanism. The isolated ra...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000350866
更新日期：2013-01-01 00:00:00
abstract：:Neointima formation participates in the pathophysiology of atherosclerosis and restenosis. Proliferation and migration of vascular smooth muscle cells (VSMC) are initial responses to vascular injury. The aim of the present study was to assess the effect of gliotoxin, an inhibitor of nuclear factor (NF)-kappaB, on migr...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000176043
更新日期：2009-01-01 00:00:00
abstract：:Although the content of histamine in myocardial tissue is high, its contribution to the regulation of coronary blood flow has not been clearly defined. The aim of the present study was to investigate whether or not nitric oxide (NO), an important modulator of coronary vascular tone, is involved in histamine-induced co...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158987
更新日期：1993-05-01 00:00:00
abstract：BACKGROUND:Lipoxin A4 (LXA4) is a biologically active product generated from arachidonic acid by lipoxygenase action. The production of lipoxins is enhanced by aspirin through acetylation of cyclooxygenase-2, via a mechanism known as 'aspirin-triggered lipoxin'. LXA4 has both anti-inflammatory and proinflammatory actio...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000371490
更新日期：2014-01-01 00:00:00
abstract：:The circadian clock is rhythmically expressed in blood vessels, but the interaction between the circadian clock and disturbed blood flow remains unclear. We examined the relationships between BMAL1 and CLOCK and 2 regulators of endothelial function, AKT1 and endothelial nitric oxide synthase (eNOS), in vascular region...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000452410
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:Hypoxia results in pulmonary hypertension and vascular remodeling due to induction of pulmonary artery cell proliferation. Besides pulmonary artery smooth muscle cells, pulmonary artery endothelial cells (PAECs) are also involved in the development of pulmonary hypertension, but the effect of hypoxia on PAEC...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000327005
更新日期：2011-01-01 00:00:00
abstract：:We investigated the possible interference of smooth muscle cells with monocyte response to LDL as well as with their adhesion and transmigration in a coculture of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to e...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051081
更新日期：2001-09-01 00:00:00
abstract：:Studies on the possible causal relationship between the Ca2+ channel current density in the vascular muscle cell (VMC) and increases in blood pressure were extended by a comparison of stroke-prone spontaneously hypertensive rats (SP-SHR) with N/nih outbred normotensive rats. Maximal amplitudes of both L-type and T-typ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159064
更新日期：1994-11-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to assess the rat pial microvessel alterations due to transient bilateral common carotid artery occlusion (BCCAO) and to investigate the mechanism of 10% hypertonic glycerol neuroprotection. Our suggestion was that 10% glycerol solution infusion could dilate pial arterioles through ni...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000109818
更新日期：2008-01-01 00:00:00
abstract：:The D allele of the insertion (I)/deletion (D) polymorphism in the angiotensin-converting enzyme (ACE) gene and the C allele of the A1166-C polymorphism in the angiotensin II type 1 receptor (AGT1R) gene have been associated with altered vascular structure and with an increased risk of myocardial infarction. The aim o...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025687
更新日期：1999-11-01 00:00:00
abstract：:Cx40-deficient mice (Cx40-/-) are hypertensive due to increased renin secretion. We evaluated the renal expression of neuronal nitric oxide synthase (nNOS) and cyclooxygenases COX-1 and COX-2, three macula densa enzymes. The levels of nNOS were increased in kidneys of Cx40-/- mice, as well as in those of wild-type (WT...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000156704
更新日期：2009-01-01 00:00:00
abstract：:The present study investigated transcellular signalling mechanism involved in thrombin-induced production of plasminogen activator inhibitor-1 (PAI-1) in cultured vascular baboon aortic smooth muscle cells (BASMC). Treatments with thrombin dose-dependently increased the steady state levels of PAI-1 mRNA and the genera...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159205
更新日期：1997-03-01 00:00:00
abstract：:The contribution of ATP-sensitive potassium channel (KATP channel) blockade in the vasoconstriction produced by vasopressin was studied. All experiments were performed using rat thoracic aorta cut in 4-mm rings, denuded from their endothelium and mounted into 20-ml organ baths. Glibenclamide (0.01-10 microM), a KATP c...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159088
更新日期：1995-03-01 00:00:00
