Abstract:
:We investigated the possible interference of smooth muscle cells with monocyte response to LDL as well as with their adhesion and transmigration in a coculture of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to endothelial cells both in mono- and in coculture with smooth muscle cells. When THP-1 cells were incubated with endothelial cells in the presence of copper oxLDL, their adhesion was increased, but only in coculture. The addition of sodium nitroprusside (SNP) together with oxLDL markedly increased the adhesion of THP-1 cells in coculture. Close proximity between endothelial and smooth muscle cells was necessary to observe that effect. Furthermore, this increase in adhesion of THP-1 cells can, at least in part, be attributed to the augmented production of monocyte chemoattractant protein-1 (MCP-1) observed in coculture under the influence of oxLDL and SNP. The passage of THP-1 cells through the coculture was stimulated by MCP-1 and LPC. These results show that physical contacts or close proximity between endothelial and smooth muscle cells play a key role in the adhesion of monocytes and their infiltration into the intima in response to oxLDL.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Kinard F,Jaworski K,Sergent-Engelen T,Goldstein D,Van Veldhoven PP,Holvoet P,Trouet A,Schneider YJ,Remacle Cdoi
10.1159/000051081keywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
479-91issue
5eissn
1018-1172issn
1423-0135pii
51081journal_volume
38pub_type
杂志文章abstract:BACKGROUND:Lipoxin A4 (LXA4) is a biologically active product generated from arachidonic acid by lipoxygenase action. The production of lipoxins is enhanced by aspirin through acetylation of cyclooxygenase-2, via a mechanism known as 'aspirin-triggered lipoxin'. LXA4 has both anti-inflammatory and proinflammatory actio...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000371490
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:We have developed an in vitro model by organ culture of rat mesenteric arteries to imitate vascular smooth muscle cell (VSMC) receptor changes in cardiovascular disease. By using this model, alteration of VSMC thromboxane A(2) (TP) receptors was studied. METHODS AND RESULTS:After organ culture of the arteri...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000153247
更新日期:2009-01-01 00:00:00
abstract::The aim of this study was to use periarterial manipulation to produce an atheroma-like neo-intima in rabbits and study resting blood flow and vascular responsiveness in vivo. One common carotid artery was enclosed in a silastic collar to induce a neo-intima similar to that of human early atherosclerosis, and carotid b...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159043
更新日期:1994-07-01 00:00:00
abstract::The contribution of ATP-sensitive potassium channel (KATP channel) blockade in the vasoconstriction produced by vasopressin was studied. All experiments were performed using rat thoracic aorta cut in 4-mm rings, denuded from their endothelium and mounted into 20-ml organ baths. Glibenclamide (0.01-10 microM), a KATP c...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159088
更新日期:1995-03-01 00:00:00
abstract::The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn's disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from prim...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000076126
更新日期:2004-01-01 00:00:00
abstract::The influence of vascular endothelium on ouabain-induced contractions and sodium pump activity in aortic segments of Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) was analyzed. De-endothelialization increased and reduced ouabain-induced contractions in WKY and SHR segments, respectively. The effects of d...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159145
更新日期:1996-03-01 00:00:00
abstract:AIM:Transglutaminase 2 (TG2) is important for the deposition and stability of the extracellular matrix via effects on cross-linking of matrix proteins and transforming growth factor beta (TGFbeta) activity. The purpose of this study was to investigate the effect of TG2 deficiency on the composi- tion of atherosclerotic...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000255966
更新日期:2010-01-01 00:00:00
abstract::Actin cytoskeleton reorganization is regulated by various actin-binding proteins. Cofilin is the principal filament-depolymerizing protein, whose activity is reduced upon phosphorylation by LIMK. Thus, LIMK and cofilin comprise a signal transduction module regulating actin turnover and myogenic tone in healthy vascula...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000081247
更新日期:2004-09-01 00:00:00
abstract:BACKGROUND:Angiopoietin-like protein 3 (ANGPTL3) is a liver-derived plasma protein that modulates plasma triglyceride clearance, angiogenesis and atherosclerosis in experimental models. So far, no study has examined its role in atherosclerosis in human subjects. We evaluated the possible association between plasma ANGP...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000098153
更新日期:2007-01-01 00:00:00
abstract::The effect of shear stress at the endothelium in the attenuation of the noradrenaline-induced constriction of the femoral vascular bed perfused at a constant blood flow was investigated in 16 anesthetized cats. It is known that the adrenergic vasoconstriction of the femoral vascular bed is considerably greater at a co...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000097750
更新日期:2007-01-01 00:00:00
abstract::The proliferation of vascular smooth muscle cells (VSMC) plays a significant part in both the developing atherosclerotic lesion and in restenosis. Heparin has been widely reported to inhibit the growth of VSMC in culture and intimal VSMC in some animal models of vascular hyperplasia. Clinical trials with heparin, howe...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025616
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND:Hypoxia results in pulmonary hypertension and vascular remodeling due to induction of pulmonary artery cell proliferation. Besides pulmonary artery smooth muscle cells, pulmonary artery endothelial cells (PAECs) are also involved in the development of pulmonary hypertension, but the effect of hypoxia on PAEC...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000327005
更新日期:2011-01-01 00:00:00
abstract::Neointima formation participates in the pathophysiology of atherosclerosis and restenosis. Proliferation and migration of vascular smooth muscle cells (VSMC) are initial responses to vascular injury. The aim of the present study was to assess the effect of gliotoxin, an inhibitor of nuclear factor (NF)-kappaB, on migr...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000176043
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Elevated plasma homocysteine (Hcy) is a risk factor for coronary disease. The objective of this study was to investigate whether Hcy either alone or in high glucose conditions induces endothelin-1 (ET-1) synthesis via the production of reactive oxygen species (ROS). METHODS:Bovine aortic endothelial cells w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000090947
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:Increased peripheral arterial resistances and decreased maximum vasodilation are characteristic features of chronic hypertension. However, little data are available in the literature regarding the possible alterations in the temporal patterns of vasodilatory responses elicited by various stimuli. DESIGN:This...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000090944
更新日期:2006-01-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is a principal stimulator of angiogenesis. However, the downstream targets of VEGF in endothelial cells (ECs) are not entirely clarified. Survey of downstream targets of VEGF in human ECs identified a number of genes, including Down syndrome candidate region 1 (DSCR1). Here, w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000079832
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND AND AIMS:Vasomotion consists in cyclic oscillations of the arterial diameter induced by the synchronized activity of the smooth muscle cells. So far, contradictory results have emerged in the literature about the role of the endothelium in the onset and maintenance of vasomotion. Here our aim is to understan...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000124283
更新日期:2008-01-01 00:00:00
abstract::Coronary bypass vessels, saphenous vein (SV) and internal thoracic artery (ITA), differ in susceptibility to atherosclerosis and medium- to long-term patency. Whereas most ITA remain patent (90% at 10 years), 20% of SV grafts fail in the first year and approximately 45% fail within 10 years. Reasons for these differen...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159245
更新日期:1997-09-01 00:00:00
abstract::In the present study, we investigated the actions of calmodulin (CaM) and CaM-dependent protein kinase II (CaMK-II) on the L-type Ca2+ currents (ICa(L)) of cultured vascular smooth muscle (VSM) cells (A7r5 cell line), using the whole-cell voltage clamp method. The peak IBa (Ca2+ channel using 5 mM Ba2+ as charge carri...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025599
更新日期:1998-09-01 00:00:00
abstract::In coronary artery diseases, cigarette smoking is a risk factor and the endothelin system plays a key role in the pathogenesis. This study was to examine if dimethylsulfoxide-soluble smoke particles (DSP) upregulate endothelin type-B (ETB) receptors in the coronary artery and investigate the mechanism. The isolated ra...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000350866
更新日期:2013-01-01 00:00:00
abstract::The localization of dopamine receptor subtypes in the rabbit pulmonary circulation was investigated by using combined radioligand binding and light microscope autoradiography techniques. Dopamine D1-like receptors, probably belonging to the dopamine D1 receptor subtype, were demonstrated in sections of rabbit lung usi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159094
更新日期:1995-05-01 00:00:00
abstract:OBJECTIVE:It is well known that inflammation plays key roles in the development of atherosclerosis and that the transplantation of bone marrow mononuclear cells (BMMNCs) can suppress inflammation in rodent models of ischemic diseases. Here, we explored whether transplantation of autologous BMMNCs could prevent the prog...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449201
更新日期:2016-01-01 00:00:00
abstract::Therapies aimed at manipulating the microcirculation require the ability to control angiogenesis, defined as the sprouting of new capillaries from existing vessels. Blocking angiogenesis would be beneficial in many pathologies (e.g. cancer, retinopathies and rheumatoid arthritis). In others (e.g. myocardial infarction...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000362276
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND/AIMS:Endoplasmic reticulum (ER) stress has emerged as a potential mechanism contributing to diabetes and its comorbidities. However, the importance of ER stress in diabetic vascular dysfunction is unclear. The purpose of this study was to examine the effects of the ER stress inhibitor, tauroursodeoxycholic a...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000479967
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND/AIMS:The endothelium has been recognized as a key component in the regulation of blood vessels. We designed a simple procedure to separate endothelial and smooth muscle RNA from rat aorta and mesenteric artery and used this method to establish the distribution of Na(+)/K(+)-ATPase alpha-subunit isoforms (NaK...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000095963
更新日期:2006-01-01 00:00:00
abstract::It has been well established that wall shear stress is an important determinant of endothelial cell function and gene expression as well as of its structure. There is increasing evidence that low wall shear stress, as present in artery bifurcations opposite to the flow divider where atherosclerotic lesions preferentia...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000091648
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND/AIMS:The present report examines a new pig model for progressive induction of high-grade stenosis, for the study of chronic myocardial ischemia and the dynamics of collateral vessel growth. METHODS:Thirty-nine Landrace pigs were instrumented with a novel experimental stent (GVD stent) in the left anterior d...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000181545
更新日期:2009-01-01 00:00:00
abstract::Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159106
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smoot...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000318777
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induc...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000101450
更新日期:2007-01-01 00:00:00