Abstract:
:The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn's disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from primary human bone marrow aspirates, i.e. endothelial cells, and on primary human endothelial cells from cord blood. Also, immortalized human umbilical cord endothelial cells and human bone marrow endothelial cells showed ICAM-3 expression. However, its function on human endothelium is not known. Surprisingly, activation of endothelial ICAM-3 by crosslinking with specific antibodies resulted in a drop in the electrical resistance of bone marrow endothelial monolayers. In line with this, immunocytochemical analysis showed a loss of endothelial cell-cell contacts after ICAM-3 crosslinking in HBMEC. Detailed biochemical analysis showed an association of moesin and in a later stage ezrin with ICAM-3 upon crosslinking in HBMEC. Moreover, ICAM-3 crosslinking induced the production of reactive oxygen species (ROS), which are known to be involved in the control of endothelial cell-cell contacts. In conclusion, we showed that ICAM-3 is expressed on human bone marrow endothelial cells and controls endothelial integrity via ROS-dependent signaling.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
van Buul JD,Mul FP,van der Schoot CE,Hordijk PLdoi
10.1159/000076126keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
28-37issue
1eissn
1018-1172issn
1423-0135pii
76126journal_volume
41pub_type
杂志文章abstract:BACKGROUND/AIMS:The present report examines a new pig model for progressive induction of high-grade stenosis, for the study of chronic myocardial ischemia and the dynamics of collateral vessel growth. METHODS:Thirty-nine Landrace pigs were instrumented with a novel experimental stent (GVD stent) in the left anterior d...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000181545
更新日期:2009-01-01 00:00:00
abstract::Dietary flavonoids are thought to protect against cardiovascular disease. We have studied the effects of bioactive isoflavone metabolites on hyperlipidemia, endothelial dysfunction and the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE(0)) mice fed a Western high-fat diet. Supplementation w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000071891
更新日期:2003-05-01 00:00:00
abstract::Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass g...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000376548
更新日期:2015-01-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is a principal stimulator of angiogenesis. However, the downstream targets of VEGF in endothelial cells (ECs) are not entirely clarified. Survey of downstream targets of VEGF in human ECs identified a number of genes, including Down syndrome candidate region 1 (DSCR1). Here, w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000079832
更新日期:2004-07-01 00:00:00
abstract::Insulin was reported to decrease Na(+)/H(+) exchange activity in murine vascular smooth muscle (VSM) tissue. In most other cells, insulin increases activity. We tested the effects of insulin on Na(+)/H(+) exchange activity in primary cultured canine VSM cells. Intracellular pH (pH(i)) was measured with 2',7'-bis(2-car...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000329587
更新日期:2011-01-01 00:00:00
abstract::The proliferation of vascular smooth muscle cells (VSMC) plays a significant part in both the developing atherosclerotic lesion and in restenosis. Heparin has been widely reported to inhibit the growth of VSMC in culture and intimal VSMC in some animal models of vascular hyperplasia. Clinical trials with heparin, howe...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025616
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND:Extracellular matrix deposition is the main factor inducing stenotic lesions in arterial grafts. Lysyl oxidases (LOX) play a key role in stabilizing collagen and elastin. OBJECTIVE:To examine the repair response to arterial allografts in terms of LOX expression and collagen/elastin deposition using LOX inhi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000317399
更新日期:2011-01-01 00:00:00
abstract::In coronary artery diseases, cigarette smoking is a risk factor and the endothelin system plays a key role in the pathogenesis. This study was to examine if dimethylsulfoxide-soluble smoke particles (DSP) upregulate endothelin type-B (ETB) receptors in the coronary artery and investigate the mechanism. The isolated ra...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000350866
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Safety concerns associated with drug-eluting stents have spurred interest in alternative vessel therapeutics following angioplasty. Microbubble contrast agents have been shown to increase gene transfection in vivo in the presence of ultrasound. OBJECTIVES/METHODS:The purpose of this study was to determine w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000258905
更新日期:2010-01-01 00:00:00
abstract::There is evidence suggesting that fluid shear stress activates phospholipid turnover in endothelial cells, but it is not clear which phospholipids are involved in the transduction of the flow signal. Cultured human umbilical-vein endothelial cells were prelabeled with [14C]-arachidonic acid and subjected to laminar sh...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158963
更新日期:1992-11-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000461581
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induc...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000101450
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND/AIMS:The endothelium has been recognized as a key component in the regulation of blood vessels. We designed a simple procedure to separate endothelial and smooth muscle RNA from rat aorta and mesenteric artery and used this method to establish the distribution of Na(+)/K(+)-ATPase alpha-subunit isoforms (NaK...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000095963
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND/AIMS:Laser Doppler flowmetry (LDF) was used to determine the influence of acute myocardial infarction (AMI) and of successful reperfusion treatment on basal skin blood flow and its oscillatory components. METHODS:Skin LDF was performed on all extremities in 58 patients 4-9 days after AMI (Killip class I), a...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000110208
更新日期:2008-01-01 00:00:00
abstract:UNLABELLED:Nitric oxide (NO) and cyclooxygenase (COX) derived prostaglandins (PGs) are involved in vascular homeostasis. Contradictory results have been obtained in previous studies on the putative 'cross-talk' between these two pathways. Our aim was to evaluate the interaction between NO and PG release in human microv...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000074550
更新日期:2003-09-01 00:00:00
abstract::The Cytoscan Model E-II (Cytometrics Inc., Philadelphia, Pa., USA) is a newly developed instrument which functions as an intravital microscope and is small and easily portable. Through the use of orthogonal polarization spectral (OPS) imaging, the Cytoscan Model E-II delivers images of the microcirculation which are c...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000054079
更新日期:2000-11-01 00:00:00
abstract::The Notch4 signaling pathway of endothelial progenitor cells (EPCs) may play a crucial role in Kawasaki disease (KD). We investigated the proliferation, adhesion, migration, angiogenesis, and expression levels of Notch4, recombination signal-binding protein-Jκ (RBP-Jκ), P-selectin, and vascular cell adhesion molecule-...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449061
更新日期:2016-01-01 00:00:00
abstract::The circadian clock is rhythmically expressed in blood vessels, but the interaction between the circadian clock and disturbed blood flow remains unclear. We examined the relationships between BMAL1 and CLOCK and 2 regulators of endothelial function, AKT1 and endothelial nitric oxide synthase (eNOS), in vascular region...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000452410
更新日期:2016-01-01 00:00:00
abstract::Organic nitrates act as vasodilators and have long been used for treatment of cardiovascular diseases. However, the therapeutic effect of nitrates is limited by induction of nitrate tolerance which is associated with endothelial dysfunction and enhanced vasoconstriction. Multiple mechanisms cause nitrate tolerance inc...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000065542
更新日期:2002-07-01 00:00:00
abstract::Although the content of histamine in myocardial tissue is high, its contribution to the regulation of coronary blood flow has not been clearly defined. The aim of the present study was to investigate whether or not nitric oxide (NO), an important modulator of coronary vascular tone, is involved in histamine-induced co...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158987
更新日期:1993-05-01 00:00:00
abstract::The pathophysiology of Raynaud's phenomenon (RP) remains an enigma. Whatever theories proposed, the final event leading to the clinical symptoms is the occlusion of digital vessels. However, the possibility that the upstream large arterial vessels contribute to vasopasm has never been investigated. We used a high reso...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159237
更新日期:1997-07-01 00:00:00
abstract::Previous studies have demonstrated endothelial and smooth muscle hyperplasia occur during arterial luminal expansion associated with elevation of arterial wall shear rates. The current study investigated whether remodeling induced by elevated wall shear would ultimately result in a vessel with intimal and medial cell ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025592
更新日期:1998-07-01 00:00:00
abstract::Inflammation is a highly regulated process with common but also specific characteristics in each tissue affected. Recruitment of leukocytes from the blood to the injured tissue is an important early step in the inflammatory cascade. This review highlights the role of connexins (Cxs) in the regulation of both acute and...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000316942
更新日期:2011-01-01 00:00:00
abstract::Proper development of the vascular system as one of the earliest and most critical steps during vertebrate embryogenesis is ensured by the exact spatial and temporal control of gene expression in cells forming the vessel network. Whereas the regulation of vascular system development is well elucidated on the level of ...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000353287
更新日期:2013-01-01 00:00:00
abstract::Pituitary adenylate cyclase-activating peptide (PACAP; 1-38 and 1-27) and vasoactive intestinal peptide (VIP) are related neuropeptides of the secretin/glucagon family. Overlapping signaling through G-protein-coupled receptors mediates their vasomotor activity. We previously showed that PACAP deficiency (PACAP-KO) shi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000481781
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:To determine: (1) whether variables of a pulse wave form analysis of ocular microcirculation shown by laser speckle flowgraphy (LSFG) correlate with left ventricular (LV) systolic dysfunction and (2) whether these variables correlate with LV diastolic dysfunction in subjects without LV systolic dysfunction as a...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000494066
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVE:Increased peripheral arterial resistances and decreased maximum vasodilation are characteristic features of chronic hypertension. However, little data are available in the literature regarding the possible alterations in the temporal patterns of vasodilatory responses elicited by various stimuli. DESIGN:This...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000090944
更新日期:2006-01-01 00:00:00
abstract::The contribution of ATP-sensitive potassium channel (KATP channel) blockade in the vasoconstriction produced by vasopressin was studied. All experiments were performed using rat thoracic aorta cut in 4-mm rings, denuded from their endothelium and mounted into 20-ml organ baths. Glibenclamide (0.01-10 microM), a KATP c...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159088
更新日期:1995-03-01 00:00:00
abstract:AIMS:We investigated the role of superoxide O(2)(-) during the initiation of vascular endothelial growth factor (VEGF)- and placental growth factor (PlGF)-mediated signal transduction in bone marrow-derived endothelial cells. METHODS:BMhTERT cells were treated with VEGF or PlGF in the presence or absence of antioxidan...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000226228
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND/AIMS:Endothelial colony-forming cells (ECFCs) have the potential to be used in regenerative medicine. Dysfunction of ECFCs is correlated with the onset of cardiovascular disorders, especially coronary artery disease (CAD). Binding of vascular endothelial growth factor A (VEGFA) to vascular endothelial growth...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449202
更新日期:2017-01-01 00:00:00