Abstract:
:Studies on the possible causal relationship between the Ca2+ channel current density in the vascular muscle cell (VMC) and increases in blood pressure were extended by a comparison of stroke-prone spontaneously hypertensive rats (SP-SHR) with N/nih outbred normotensive rats. Maximal amplitudes of both L-type and T-type Ca2+ channel currents were significantly increased in SP-SHR without a difference in cell capacitance. SP-SHR peak current amplitudes in 20 mM Ba2+ averaged 446 +/- 64 pA while N/nih averaged 156 +/- 25 pA (clearly separated statistically). Both L-type and T-type Ba2+ currents (IBa) were significantly increased in SP-SHR, shown also by peak current frequency distributions. There was a significant shift to the left of both activation (7 mV) and inactivation (15 mV) current-voltage (I-V) plots. SP-SHR IBa recovery from inactivation was significantly slower (103 versus 61 ms) than in N/nih VMC. The increases in SP-SHR IBa amplitude under maximized conditions correlated with increases in blood pressure. Together with earlier observations of increased vascular muscle Ca2+ current density coexistent with blood pressure elevation in Kyoto-Wistar SHR, these data provide evidence for altered function of Ca2+ channels as a fundamental component of hypertension. Since the Ca2+ channel alterations exist in venous VMCs of newborn SP-SHR rats (in a low pressure blood vessel and at a time when increased Ca2+ current density could not be an effect of increased blood pressure), our results add to the growing evidence of Ca2+ channel abnormalities as a cause of genetic hypertension.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Self DA,Bian K,Mishra SK,Hermsmeyer Kdoi
10.1159/000159064subject
Has Abstractpub_date
1994-11-01 00:00:00pages
359-66issue
6eissn
1018-1172issn
1423-0135journal_volume
31pub_type
杂志文章abstract::The aim of this study was to verify that subcutaneous lymph node transplantation inducing lymphatic regeneration is possible in healthy adult rats, in analogy to results obtained in other species. This rat model was used to determine the effects of lymph node fragmentation as well as sheep erythrocytes and platelet-ri...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000194269
更新日期:2009-01-01 00:00:00
abstract::In the present study, we investigated the actions of calmodulin (CaM) and CaM-dependent protein kinase II (CaMK-II) on the L-type Ca2+ currents (ICa(L)) of cultured vascular smooth muscle (VSM) cells (A7r5 cell line), using the whole-cell voltage clamp method. The peak IBa (Ca2+ channel using 5 mM Ba2+ as charge carri...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025599
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:Controversial reports on prolactin receptors (PRL-R), the long and short form, on endothelial cells (EC) may be explained by the choice of EC derived from the micro- and macrovascular bed of either endocrine and non-endocrine organs. METHODS:We studied here PRL-R expression in organs [bovine corpus luteum (...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000097892
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Transfer of molecular signals from veins to adjacent arteries is an established mechanism of vascular communication in the uterine circulation, which ultimately depends on venous permeability. This study tests the hypotheses that: (1) uterine veins are permeable to intravenous solutes, using a 3-kDa dextran ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000082976
更新日期:2005-01-01 00:00:00
abstract::We examined the growth of the right common carotid artery of young rabbits after ligating the left common carotid artery at 3 weeks of age, a procedure that approximately doubled right carotid blood flows. Flow increased from 0.065 +/-0.003 to 0.096+/-0.009 ml/s within 1 h and, at 15 weeks of age, carotid blood flows ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025559
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:We have developed an in vitro model by organ culture of rat mesenteric arteries to imitate vascular smooth muscle cell (VSMC) receptor changes in cardiovascular disease. By using this model, alteration of VSMC thromboxane A(2) (TP) receptors was studied. METHODS AND RESULTS:After organ culture of the arteri...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000153247
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Lipoxin A4 (LXA4) is a biologically active product generated from arachidonic acid by lipoxygenase action. The production of lipoxins is enhanced by aspirin through acetylation of cyclooxygenase-2, via a mechanism known as 'aspirin-triggered lipoxin'. LXA4 has both anti-inflammatory and proinflammatory actio...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000371490
更新日期:2014-01-01 00:00:00
abstract::Cx40-deficient mice (Cx40-/-) are hypertensive due to increased renin secretion. We evaluated the renal expression of neuronal nitric oxide synthase (nNOS) and cyclooxygenases COX-1 and COX-2, three macula densa enzymes. The levels of nNOS were increased in kidneys of Cx40-/- mice, as well as in those of wild-type (WT...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000156704
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to assess the rat pial microvessel alterations due to transient bilateral common carotid artery occlusion (BCCAO) and to investigate the mechanism of 10% hypertonic glycerol neuroprotection. Our suggestion was that 10% glycerol solution infusion could dilate pial arterioles through ni...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000109818
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Safety concerns associated with drug-eluting stents have spurred interest in alternative vessel therapeutics following angioplasty. Microbubble contrast agents have been shown to increase gene transfection in vivo in the presence of ultrasound. OBJECTIVES/METHODS:The purpose of this study was to determine w...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000258905
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVES:Extracellular nucleotide release at the site of arterial injury mediates the proliferation and migration of vascular smooth muscle cells. Our aim was to investigate the role of the P2Y2 nucleotide receptor (P2Y2R) in neointimal hyperplasia. Approach and Results: Vascular injury was induced by the implantatio...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449059
更新日期:2016-01-01 00:00:00
abstract::Previous studies have demonstrated endothelial and smooth muscle hyperplasia occur during arterial luminal expansion associated with elevation of arterial wall shear rates. The current study investigated whether remodeling induced by elevated wall shear would ultimately result in a vessel with intimal and medial cell ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025592
更新日期:1998-07-01 00:00:00
abstract:AIMS:Percutaneous coronary intervention is routinely performed to treat occlusive coronary artery disease. Coronary perforation is a potential complication and can be treated with a stent graft. Current stent grafts are associated with high restenosis rates. We tested the safety and feasibility of biodegradable stent g...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000505454
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND/AIMS:Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress in resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of the membrane-bound enzyme to Golgi ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000355301
更新日期:2013-01-01 00:00:00
abstract::Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) are cytoplasmic adaptor proteins of the TNF/interleukin (IL)-1/Toll-like receptor superfamily. Ligands of this family such as TNFα, CD40L, and IL-1β promote chronic inflammatory processes such as atherosclerosis and restenosis, the latter being a common a...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000501615
更新日期:2019-01-01 00:00:00
abstract::Regulation of [Ca2+]i and modulation of the [Ca2+]i sensitivity of myosin phosphorylation in smooth muscles may involve phosphorylation of one or more proteins on tyrosine residues. We tested this hypothesis by measuring tyrosine phosphorylation of proteins extracted from swine carotid artery and separated by SDS gel ...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159196
更新日期:1997-01-01 00:00:00
abstract:PURPOSE:Hypoxic conditions cause fibroblasts to differentiate into alpha smooth-muscle cell actin (α -SMA)-positive cells, i.e. myofibroblasts. This process is a hallmark of venous neointimal hyperplasia (VNH) associated with hemodialysis vascular access. The purpose of this study was to determine if blood outgrowth en...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000369929
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:Exercise elicits early adaptation of coronary vessels enabling the coronary circulation to respond adequately to higher flow demands. We hypothesized that short-term daily exercise induces biomechanical and functional remodeling of the coronary resistance arteries related to pressure. METHODS:Male rats were su...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000486571
更新日期:2018-01-01 00:00:00
abstract::The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn's disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from prim...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000076126
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND/AIMS:The endothelium has been recognized as a key component in the regulation of blood vessels. We designed a simple procedure to separate endothelial and smooth muscle RNA from rat aorta and mesenteric artery and used this method to establish the distribution of Na(+)/K(+)-ATPase alpha-subunit isoforms (NaK...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000095963
更新日期:2006-01-01 00:00:00
abstract::In response to stimuli, endothelial cells release arachidonic acid, a lipid precursor of various vasoactive substances. We have investigated the relationships between cytosolic Ca2+ movements and arachidonic acid release in human umbilical vein endothelial cells. Histamine, a receptor-dependent agonist, and thapsigarg...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025647
更新日期:1999-05-01 00:00:00
abstract::The pH has marked effects on the blood flow in several vascular beds but the underlying mechanisms remain incompletely understood. It is still not agreed, for example, whether it is the fall in extracellular pH or intracellular pH that is responsible for changes in tone resulting from hypercapnic acidosis. The issue h...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000159163
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smoot...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000318777
更新日期:2011-01-01 00:00:00
abstract::We evaluated the effects of a simulated workday of prolonged sitting on blood pressure (BP) and pulse wave velocity (PWV) and examined whether posture (seated vs. supine) affected responses. Participants (n = 25) were adults, with overweight/obesity and elevated BP, and performed seated desk work for 7.5 h. BP and PWV...
journal_title:Journal of vascular research
pub_type: 杂志文章,随机对照试验
doi:10.1159/000510294
更新日期:2020-01-01 00:00:00
abstract::Neointima formation participates in the pathophysiology of atherosclerosis and restenosis. Proliferation and migration of vascular smooth muscle cells (VSMC) are initial responses to vascular injury. The aim of the present study was to assess the effect of gliotoxin, an inhibitor of nuclear factor (NF)-kappaB, on migr...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000176043
更新日期:2009-01-01 00:00:00
abstract::Therapies aimed at manipulating the microcirculation require the ability to control angiogenesis, defined as the sprouting of new capillaries from existing vessels. Blocking angiogenesis would be beneficial in many pathologies (e.g. cancer, retinopathies and rheumatoid arthritis). In others (e.g. myocardial infarction...
journal_title:Journal of vascular research
pub_type: 杂志文章,评审
doi:10.1159/000362276
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Extracellular matrix deposition is the main factor inducing stenotic lesions in arterial grafts. Lysyl oxidases (LOX) play a key role in stabilizing collagen and elastin. OBJECTIVE:To examine the repair response to arterial allografts in terms of LOX expression and collagen/elastin deposition using LOX inhi...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000317399
更新日期:2011-01-01 00:00:00
abstract::Although most physiologically important processes are regulated through negative feedback loops and numerous factors regulating endothelial apoptosis are identified, little is known about negative feedback mechanisms for endothelial apoptosis. Here we describe the release of soluble anti-apoptotic activity by endothel...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000111072
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Angiopoietin-like protein 3 (ANGPTL3) is a liver-derived plasma protein that modulates plasma triglyceride clearance, angiogenesis and atherosclerosis in experimental models. So far, no study has examined its role in atherosclerosis in human subjects. We evaluated the possible association between plasma ANGP...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000098153
更新日期:2007-01-01 00:00:00
abstract::Although the content of histamine in myocardial tissue is high, its contribution to the regulation of coronary blood flow has not been clearly defined. The aim of the present study was to investigate whether or not nitric oxide (NO), an important modulator of coronary vascular tone, is involved in histamine-induced co...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158987
更新日期:1993-05-01 00:00:00