Abstract：
BACKGROUND:Controversial reports on prolactin receptors (PRL-R), the long and short form, on endothelial cells (EC) may be explained by the choice of EC derived from the micro- and macrovascular bed of either endocrine and non-endocrine organs. METHODS:We studied here PRL-R expression in organs [bovine corpus luteum (CL), umbilical vein, aorta] and in organ-derived EC cultures. RESULTS:In the intact CL, both PRL-R forms were present at mRNA and protein level throughout the oestrous cycle stages. The short form prevailed as protein. PRL-R-positive EC were noted by immunofluorescent staining in arterial blood vessels of CL septa, in the umbilical vein and the aorta. In EC cultures of micro- and macrovascular origin, transcripts of both PRL-R forms were shown; again the short-form protein prevailed. Blocking experiments with anti-prolactin (PRL) antibody led to a 60% decrease in cell growth. Treatment with PRL had no effect. CONCLUSION:PRL-R expression in micro- and macrovascular EC is associated with the predominant short form.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Ricken AM,Traenkner A,Merkwitz C,Hummitzsch K,Grosche J,Spanel-Borowski Kdoi
10.1159/000097892subject
Has Abstractpub_date
2007-01-01 00:00:00pages
19-30issue
1eissn
1018-1172issn
1423-0135pii
000097892journal_volume
44pub_type
杂志文章abstract：BACKGROUND:A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smoot...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000318777
更新日期：2011-01-01 00:00:00
abstract：:The present study investigated transcellular signalling mechanism involved in thrombin-induced production of plasminogen activator inhibitor-1 (PAI-1) in cultured vascular baboon aortic smooth muscle cells (BASMC). Treatments with thrombin dose-dependently increased the steady state levels of PAI-1 mRNA and the genera...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159205
更新日期：1997-03-01 00:00:00
abstract：:Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass g...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000376548
更新日期：2015-01-01 00:00:00
abstract：INTRODUCTION:This study aimed to investigate whether epigallocatechin-3-gallate (EGCG) shows antioxidant activity against angiotensin II (Ang II)-induced human umbilical vein endothelial cell (HUVEC) apoptosis. MATERIALS AND METHODS:The viability of HUVECs was revealed by MTT and LDH assay. The cell apoptosis was dete...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000479873
更新日期：2017-01-01 00:00:00
abstract：:Most virus-infected cells release interferon-beta (IFN-beta) as a powerful inducer of antiviral defense. Endothelial cells tightly regulate local immune cell recruitment by expression of adhesion molecules and chemokines. Here, we studied the transcriptional regulation of IFN-beta-induced chemokine expression in prima...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000097977
更新日期：2007-01-01 00:00:00
abstract：:Mechanical forces generated at the endothelium by fluid shear stress and pulsatile stretch are important in ensuring the continuous release of vasoactive endothelial autacoids. Although the mechanism by which endothelial cells are able to detect and convert these physical stimuli into chemical signals is unclear, this...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000025568
更新日期：1998-03-01 00:00:00
abstract：BACKGROUND:Extracellular matrix deposition is the main factor inducing stenotic lesions in arterial grafts. Lysyl oxidases (LOX) play a key role in stabilizing collagen and elastin. OBJECTIVE:To examine the repair response to arterial allografts in terms of LOX expression and collagen/elastin deposition using LOX inhi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000317399
更新日期：2011-01-01 00:00:00
abstract：:The effects of the close arterial infusion of histamine upon the microcirculation of facial and nasal tissues were examined in dogs. Blood flow through arteriovenous anastomoses (AVA flow), capillaries (CAP flow) and collaterals (COL flow) were determined by electromagnetic flowmetry and the tracer-microsphere techniq...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158972
更新日期：1993-01-01 00:00:00
abstract：:The aim of this study was to verify that subcutaneous lymph node transplantation inducing lymphatic regeneration is possible in healthy adult rats, in analogy to results obtained in other species. This rat model was used to determine the effects of lymph node fragmentation as well as sheep erythrocytes and platelet-ri...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000194269
更新日期：2009-01-01 00:00:00
abstract：:Insulin was reported to decrease Na(+)/H(+) exchange activity in murine vascular smooth muscle (VSM) tissue. In most other cells, insulin increases activity. We tested the effects of insulin on Na(+)/H(+) exchange activity in primary cultured canine VSM cells. Intracellular pH (pH(i)) was measured with 2',7'-bis(2-car...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000329587
更新日期：2011-01-01 00:00:00
abstract：:Ruptured atherosclerotic plaques, lined with activated platelets, constitute an attractive target for magnetic resonance imaging (MRI). This study evaluated whether microparticles of iron oxide (MPIO) targeting ligand-induced binding sites (LIBS) on the activated conformation of glycoprotein IIb/IIIa could be used to ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000135660
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND:Elevated plasma homocysteine (Hcy) is a risk factor for coronary disease. The objective of this study was to investigate whether Hcy either alone or in high glucose conditions induces endothelin-1 (ET-1) synthesis via the production of reactive oxygen species (ROS). METHODS:Bovine aortic endothelial cells w...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000090947
更新日期：2006-01-01 00:00:00
abstract：:Fibroblast growth factor-2 (FGF2) and vascular endothelial growth factor (VEGF) exert their angiogenic activity by interacting with endothelial cells in a distinct manner. In this study, we investigated the morphological features of endothelial cells of the chick embryo chorioallantoic membrane (CAM) microvasculature ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051070
更新日期：2001-07-01 00:00:00
abstract：:Signaling pathways activated by the tachykinin substance P (SP) were investigated in pig coronary artery endothelial cells (PCAECs). Single cells were obtained after enzymatic digestion of coronary arteries. Intracellular Ca2+ ([Ca2+]i) was measured from fura-2 fluorescence while membrane potential or ionic current wa...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159222
更新日期：1997-05-01 00:00:00
abstract：:This study utilised catecholamine fluorescence histochemistry, multiple-labelling immunohistochemistry and retrograde axonal transport to determine the distribution and neuropeptide content of sympathetic neurons innervating veins in the dorsal skin of the guinea pig pinna. There was a dense plexus of sympathetic axon...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159113
更新日期：1995-11-01 00:00:00
abstract：:The purpose of this study was to determine the contribution of long-chain fatty acids relative to other mitochondrial substrates in active vascular smooth muscle (VSM). Hog carotid arteries were isometrically contracted in physiological saline solution containing 0.71 mM U-(13)C-oleic acid (bound to albumin at a ratio...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051056
更新日期：2001-05-01 00:00:00
abstract：:The purpose of the present study was to develop a rapid, reproducible method of nonviral gene transfer to the intact vasculature. Male Sprague-Dawley rats were anesthetized, a midline abdominal incision was made and segmental branches of the superior mesenteric artery were dissected free of surrounding mesentery. A sp...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025753
更新日期：2000-09-01 00:00:00
abstract：OBJECTIVE:It is well known that inflammation plays key roles in the development of atherosclerosis and that the transplantation of bone marrow mononuclear cells (BMMNCs) can suppress inflammation in rodent models of ischemic diseases. Here, we explored whether transplantation of autologous BMMNCs could prevent the prog...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449201
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND/AIMS:Laser Doppler flowmetry (LDF) was used to determine the influence of acute myocardial infarction (AMI) and of successful reperfusion treatment on basal skin blood flow and its oscillatory components. METHODS:Skin LDF was performed on all extremities in 58 patients 4-9 days after AMI (Killip class I), a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000110208
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVES:Extracellular nucleotide release at the site of arterial injury mediates the proliferation and migration of vascular smooth muscle cells. Our aim was to investigate the role of the P2Y2 nucleotide receptor (P2Y2R) in neointimal hyperplasia. Approach and Results: Vascular injury was induced by the implantatio...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449059
更新日期：2016-01-01 00:00:00
abstract：:The influence of vascular endothelium on ouabain-induced contractions and sodium pump activity in aortic segments of Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) was analyzed. De-endothelialization increased and reduced ouabain-induced contractions in WKY and SHR segments, respectively. The effects of d...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159145
更新日期：1996-03-01 00:00:00
abstract：UNLABELLED:Nitric oxide (NO) and cyclooxygenase (COX) derived prostaglandins (PGs) are involved in vascular homeostasis. Contradictory results have been obtained in previous studies on the putative 'cross-talk' between these two pathways. Our aim was to evaluate the interaction between NO and PG release in human microv...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000074550
更新日期：2003-09-01 00:00:00
abstract：:The contribution of ATP-sensitive potassium channel (KATP channel) blockade in the vasoconstriction produced by vasopressin was studied. All experiments were performed using rat thoracic aorta cut in 4-mm rings, denuded from their endothelium and mounted into 20-ml organ baths. Glibenclamide (0.01-10 microM), a KATP c...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159088
更新日期：1995-03-01 00:00:00
abstract：OBJECTIVE:Increased peripheral arterial resistances and decreased maximum vasodilation are characteristic features of chronic hypertension. However, little data are available in the literature regarding the possible alterations in the temporal patterns of vasodilatory responses elicited by various stimuli. DESIGN:This...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000090944
更新日期：2006-01-01 00:00:00
abstract：:We investigated the possible interference of smooth muscle cells with monocyte response to LDL as well as with their adhesion and transmigration in a coculture of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to e...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051081
更新日期：2001-09-01 00:00:00
abstract：:The circadian clock is rhythmically expressed in blood vessels, but the interaction between the circadian clock and disturbed blood flow remains unclear. We examined the relationships between BMAL1 and CLOCK and 2 regulators of endothelial function, AKT1 and endothelial nitric oxide synthase (eNOS), in vascular region...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000452410
更新日期：2016-01-01 00:00:00
abstract：PURPOSE:To determine: (1) whether variables of a pulse wave form analysis of ocular microcirculation shown by laser speckle flowgraphy (LSFG) correlate with left ventricular (LV) systolic dysfunction and (2) whether these variables correlate with LV diastolic dysfunction in subjects without LV systolic dysfunction as a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000494066
更新日期：2018-01-01 00:00:00
abstract：:We examined the growth of the right common carotid artery of young rabbits after ligating the left common carotid artery at 3 weeks of age, a procedure that approximately doubled right carotid blood flows. Flow increased from 0.065 +/-0.003 to 0.096+/-0.009 ml/s within 1 h and, at 15 weeks of age, carotid blood flows ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025559
更新日期：1998-01-01 00:00:00
abstract：:Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159106
更新日期：1995-09-01 00:00:00
abstract：:In coronary artery diseases, cigarette smoking is a risk factor and the endothelin system plays a key role in the pathogenesis. This study was to examine if dimethylsulfoxide-soluble smoke particles (DSP) upregulate endothelin type-B (ETB) receptors in the coronary artery and investigate the mechanism. The isolated ra...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000350866
更新日期：2013-01-01 00:00:00
