Interaction between nitric oxide and cyclooxygenase pathways in endothelial cells.

Abstract:

UNLABELLED:Nitric oxide (NO) and cyclooxygenase (COX) derived prostaglandins (PGs) are involved in vascular homeostasis. Contradictory results have been obtained in previous studies on the putative 'cross-talk' between these two pathways. Our aim was to evaluate the interaction between NO and PG release in human microvascular endothelial cells (HMEC-1) and in umbilical vein endothelial cells (HUVEC). METHODS:Medium samples were assayed for nitrite/nitrate (NO(x)) and L-citrulline levels while lysed cells were assayed for endothelial NO synthase (eNOS), as markers of NO production. Prostaglandin E2 (PGE2) and 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) were assessed as indicators of COX activity. RESULTS:The NO donor sodium nitroprusside and L-arginine increased PGs levels in both cell types. N(G)-monomethyl-L-arginine (L-NMMA) significantly inhibited 6-keto-PGF1alpha release without significantly reducing PGE2)levels. Indomethacin increased both NO(x), eNOS and L-citrulline levels. PGE2 treatment did not modify NO(x) values. CONCLUSION:The stimulation of PGs by NO may represent an additional pathway used by exogenous nitrovasodilators to elicit vasodilation. Reduction of PGs by inhibition of COX was compensated by enhanced NO. Conversely, PGs()did not compensate()decreased NO following L-NMMA treatment. Treatment with the vasodilatator prostaglandin E2 did not modify NO(x) levels.

journal_name

J Vasc Res

authors

Vassalle C,Domenici C,Lubrano V,L'Abbate A

doi

10.1159/000074550

keywords:

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

491-9

issue

5

eissn

1018-1172

issn

1423-0135

pii

74550

journal_volume

40

pub_type

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