Abstract：
:Ruptured atherosclerotic plaques, lined with activated platelets, constitute an attractive target for magnetic resonance imaging (MRI). This study evaluated whether microparticles of iron oxide (MPIO) targeting ligand-induced binding sites (LIBS) on the activated conformation of glycoprotein IIb/IIIa could be used to image platelets. MPIO (size: 1 microm) were conjugated to anti-LIBS or control single-chain antibody. Following guidewire injury to mouse femoral artery, platelet adhesion was present after 24 h. Mice were perfused with anti-LIBS-MPIO (or control MPIO) via the left ventricle and 11.7-tesla MRI was performed on femoral arteries ex vivo. A 3D gradient echo sequence attained an isotropic resolution of 25 microm. MPIO binding, quantified by MRI, was 4-fold higher with anti-LIBS-MPIO in comparison to control MPIO (p < 0.01). In histological sections, low signal zones on MRI and MPIO correlated strongly (R(2) = 0.72; p < 0.001), indicating accurate MR quantification. In conclusion, anti-LIBS-MPIO bind to activated platelets in mouse arteries, providing a basis for the use of function-specific single-chain antibody-MPIO conjugates for molecular MRI, and represent the first molecular imaging of a conformational change in a surface receptor. This presents an opportunity to specifically image activated platelets involved in acute atherothrombosis with MRI.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
von zur Muhlen C,Peter K,Ali ZA,Schneider JE,McAteer MA,Neubauer S,Channon KM,Bode C,Choudhury RPdoi
10.1159/000135660subject
Has Abstractpub_date
2009-01-01 00:00:00pages
6-14issue
1eissn
1018-1172issn
1423-0135pii
000135660journal_volume
46pub_type
杂志文章abstract：UNLABELLED:Diabetic patients have an increased risk of restenosis and late stent thrombosis after angioplasty, i.e. complications that are related to a defective re-endothelialization. Exendin-4, a stable glucagon-like peptide (GLP)-1 receptor agonist, has been suggested to influence the formation of intimal hyperplasi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000381097
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:Elevated plasma homocysteine (Hcy) is a risk factor for coronary disease. The objective of this study was to investigate whether Hcy either alone or in high glucose conditions induces endothelin-1 (ET-1) synthesis via the production of reactive oxygen species (ROS). METHODS:Bovine aortic endothelial cells w...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000090947
更新日期：2006-01-01 00:00:00
abstract：:The influence of vascular endothelium on ouabain-induced contractions and sodium pump activity in aortic segments of Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) was analyzed. De-endothelialization increased and reduced ouabain-induced contractions in WKY and SHR segments, respectively. The effects of d...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159145
更新日期：1996-03-01 00:00:00
abstract：:Pituitary adenylate cyclase-activating peptide (PACAP; 1-38 and 1-27) and vasoactive intestinal peptide (VIP) are related neuropeptides of the secretin/glucagon family. Overlapping signaling through G-protein-coupled receptors mediates their vasomotor activity. We previously showed that PACAP deficiency (PACAP-KO) shi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000481781
更新日期：2017-01-01 00:00:00
abstract：:Previous studies have demonstrated endothelial and smooth muscle hyperplasia occur during arterial luminal expansion associated with elevation of arterial wall shear rates. The current study investigated whether remodeling induced by elevated wall shear would ultimately result in a vessel with intimal and medial cell ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025592
更新日期：1998-07-01 00:00:00
abstract：:In segments of rabbit basilar artery (BA) platelet-activating factor (PAF) initiated a concentration-dependent transitory contraction which was unaffected by indomethacin or nordihydroguaiaretic acid (NDGA). N omega-nitro-L-arginine (NLA) did not change the magnitude of PAF-induced contraction, but in the presence of ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159077
更新日期：1995-01-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is a principal stimulator of angiogenesis. However, the downstream targets of VEGF in endothelial cells (ECs) are not entirely clarified. Survey of downstream targets of VEGF in human ECs identified a number of genes, including Down syndrome candidate region 1 (DSCR1). Here, w...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000079832
更新日期：2004-07-01 00:00:00
abstract：:Previous studies demonstrated that maintenance of steady-state myogenic tone requires Ca(2+)-dependent myosin phosphorylation. The present studies furthered these observations by examining temporal relationships among Ca(2+), myosin phosphorylation and vessel diameter during acute increases in intraluminal pressure an...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000054089
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND:Transfer of molecular signals from veins to adjacent arteries is an established mechanism of vascular communication in the uterine circulation, which ultimately depends on venous permeability. This study tests the hypotheses that: (1) uterine veins are permeable to intravenous solutes, using a 3-kDa dextran ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000082976
更新日期：2005-01-01 00:00:00
abstract：:Signaling pathways activated by the tachykinin substance P (SP) were investigated in pig coronary artery endothelial cells (PCAECs). Single cells were obtained after enzymatic digestion of coronary arteries. Intracellular Ca2+ ([Ca2+]i) was measured from fura-2 fluorescence while membrane potential or ionic current wa...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159222
更新日期：1997-05-01 00:00:00
abstract：:Protein localization in endothelial cells is tightly regulated to create distinct signaling domains within their tight spatial restrictions including luminal membranes, abluminal membranes, and interendothelial junctions, as well as caveolae and calcium signaling domains. Protein localization in endothelial cells is a...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000512618
更新日期：2021-01-27 00:00:00
abstract：BACKGROUND/AIMS:Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress in resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of the membrane-bound enzyme to Golgi ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000355301
更新日期：2013-01-01 00:00:00
abstract：:Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159106
更新日期：1995-09-01 00:00:00
abstract：:Studies on the possible causal relationship between the Ca2+ channel current density in the vascular muscle cell (VMC) and increases in blood pressure were extended by a comparison of stroke-prone spontaneously hypertensive rats (SP-SHR) with N/nih outbred normotensive rats. Maximal amplitudes of both L-type and T-typ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159064
更新日期：1994-11-01 00:00:00
abstract：:The Notch4 signaling pathway of endothelial progenitor cells (EPCs) may play a crucial role in Kawasaki disease (KD). We investigated the proliferation, adhesion, migration, angiogenesis, and expression levels of Notch4, recombination signal-binding protein-Jκ (RBP-Jκ), P-selectin, and vascular cell adhesion molecule-...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449061
更新日期：2016-01-01 00:00:00
abstract：:It has been well established that wall shear stress is an important determinant of endothelial cell function and gene expression as well as of its structure. There is increasing evidence that low wall shear stress, as present in artery bifurcations opposite to the flow divider where atherosclerotic lesions preferentia...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000091648
更新日期：2006-01-01 00:00:00
abstract：:The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn's disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from prim...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000076126
更新日期：2004-01-01 00:00:00
abstract：:We evaluated the effects of a simulated workday of prolonged sitting on blood pressure (BP) and pulse wave velocity (PWV) and examined whether posture (seated vs. supine) affected responses. Participants (n = 25) were adults, with overweight/obesity and elevated BP, and performed seated desk work for 7.5 h. BP and PWV...
journal_title：Journal of vascular research
pub_type： 杂志文章,随机对照试验
doi：10.1159/000510294
更新日期：2020-01-01 00:00:00
abstract：:Actions of the novel Ca2+ antagonist, Ro 40-5967, which displays unusual efficacy against endothelin (ET)-induced contractions, were studied in isolated vascular muscle cells (VMCs) using the fluorescent protein kinase C (PKC) indicator, BODIPY 12 alpha-phorbol ester 13 beta-acetate (PBA-BODIPY). High-sensitivity (pho...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159134
更新日期：1996-01-01 00:00:00
abstract：:Inflammation is a highly regulated process with common but also specific characteristics in each tissue affected. Recruitment of leukocytes from the blood to the injured tissue is an important early step in the inflammatory cascade. This review highlights the role of connexins (Cxs) in the regulation of both acute and...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000316942
更新日期：2011-01-01 00:00:00
abstract：:Topical application of elastase to induce arterial aneurysm formation is an emerging murine model of vascular disease. In the context of aortic abdominal aneurysm (AAA), angiotensin II infusion and porcine pancreatic elastase perfusion models are commonly used today. This study, therefore, compares matrix remodeling, ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000447263
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:Safety concerns associated with drug-eluting stents have spurred interest in alternative vessel therapeutics following angioplasty. Microbubble contrast agents have been shown to increase gene transfection in vivo in the presence of ultrasound. OBJECTIVES/METHODS:The purpose of this study was to determine w...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000258905
更新日期：2010-01-01 00:00:00
abstract：:The novel endothelial cell tyrosine kinase receptors, Tie1 and Tie2, are essential for vascular development and remodeling in the embryo but little is known regarding the regulation of their expression and their role in the maintenance and repair of the adult vascular system. We examined the expression of Tie1 and Tie...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025655
更新日期：1999-07-01 00:00:00
abstract：AIMS:Percutaneous coronary intervention is routinely performed to treat occlusive coronary artery disease. Coronary perforation is a potential complication and can be treated with a stent graft. Current stent grafts are associated with high restenosis rates. We tested the safety and feasibility of biodegradable stent g...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000505454
更新日期：2020-01-01 00:00:00
abstract：:This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4 degrees C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2 alp...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159097
更新日期：1995-07-01 00:00:00
abstract：:This study utilised catecholamine fluorescence histochemistry, multiple-labelling immunohistochemistry and retrograde axonal transport to determine the distribution and neuropeptide content of sympathetic neurons innervating veins in the dorsal skin of the guinea pig pinna. There was a dense plexus of sympathetic axon...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159113
更新日期：1995-11-01 00:00:00
abstract：:Intima-media thickening (IMT) of the carotid artery, a form of vascular remodeling, correlates well with coronary artery disease risk in humans. Vascular remodeling in response to blood flow is a complex process that critically involves altered cell matrix interactions. To gain insight into these events, we performed ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000081804
更新日期：2004-11-01 00:00:00
abstract：:The purpose of this study was to determine the contribution of long-chain fatty acids relative to other mitochondrial substrates in active vascular smooth muscle (VSM). Hog carotid arteries were isometrically contracted in physiological saline solution containing 0.71 mM U-(13)C-oleic acid (bound to albumin at a ratio...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051056
更新日期：2001-05-01 00:00:00
abstract：:Chemokines play an important role in the process of leukocyte recruitment and activation at sites of inflammation. Until recently, the actions of chemokines and the expression of their receptors have only been described on different leukocyte populations. However, increasing evidence has suggested that non-haematopoie...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000025707
更新日期：2000-01-01 00:00:00
abstract：:The aim of this study was to use periarterial manipulation to produce an atheroma-like neo-intima in rabbits and study resting blood flow and vascular responsiveness in vivo. One common carotid artery was enclosed in a silastic collar to induce a neo-intima similar to that of human early atherosclerosis, and carotid b...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159043
更新日期：1994-07-01 00:00:00
