Abstract：
:Insulin was reported to decrease Na(+)/H(+) exchange activity in murine vascular smooth muscle (VSM) tissue. In most other cells, insulin increases activity. We tested the effects of insulin on Na(+)/H(+) exchange activity in primary cultured canine VSM cells. Intracellular pH (pH(i)) was measured with 2',7'-bis(2-carboxyethyl)-5-carboxyfluorescein fluorescence and Na(+) uptake by isotopic methods. Insulin alone did not significantly affect pH(i) (7.13 ± 0.05 vs. 7.10 ± 0.03 for control and insulin, respectively; p = not significant), and EIPA alone lowered it to 6.98 ± 0.04 (p < 0.05), upon which insulin lowered it further to 6.91 ± 0.04 (p < 0.05). In the presence of a pH(i) clamp, pH(i)/extracellular pH (pH(o)) 7.1/7.4, insulin increased amiloride-sensitive (22)Na uptake by 98 ± 25% (p < 0.05). At pH(i)/pH(o) 6.0/7.4 or 6.7/7.4, amiloride-sensitive (22)Na uptake was stimulated by 378 ± 59 and 105 ± 27%, respectively, compared to pH(i)/pH(o) 7.1/7.4 (p < 0.05 for all 3 versus each other), but was insulin insensitive. In acid-loaded cells (pH(i) 6.0), addition of extracellular Na(+) (pH(o) 7.4) caused rapid intracellular alkalinization, and the initial rate was not affected by insulin. It is concluded that insulin stimulates acid accumulation in VSM cells that is normally effluxed by increased Na(+)/H(+) exchange activity, and insulin may directly stimulate Na(+)/H(+) exchange activity when cells are clamped at resting pH(i) but not at acidic pH(i).
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Kahn AM,Yang Mdoi
10.1159/000329587subject
Has Abstractpub_date
2011-01-01 00:00:00pages
505-12issue
6eissn
1018-1172issn
1423-0135pii
000329587journal_volume
48pub_type
杂志文章abstract：BACKGROUND AND AIMS:Vasomotion consists in cyclic oscillations of the arterial diameter induced by the synchronized activity of the smooth muscle cells. So far, contradictory results have emerged in the literature about the role of the endothelium in the onset and maintenance of vasomotion. Here our aim is to understan...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000124283
更新日期：2008-01-01 00:00:00
abstract：:Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159106
更新日期：1995-09-01 00:00:00
abstract：:We tested the hypothesis that oxidized low-density lipoprotein (oxLDL)-induced inactivation of Akt within endothelial progenitor cells (EPCs) is mediated at the level of phosphoinositide 3-kinase (PI3K), specifically by nitrosylation of the p85 subunit of PI3K, and that this action is critical in provoking oxLDL-induc...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000313879
更新日期：2010-01-01 00:00:00
abstract：:Cx40-deficient mice (Cx40-/-) are hypertensive due to increased renin secretion. We evaluated the renal expression of neuronal nitric oxide synthase (nNOS) and cyclooxygenases COX-1 and COX-2, three macula densa enzymes. The levels of nNOS were increased in kidneys of Cx40-/- mice, as well as in those of wild-type (WT...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000156704
更新日期：2009-01-01 00:00:00
abstract：:Studies on the possible causal relationship between the Ca2+ channel current density in the vascular muscle cell (VMC) and increases in blood pressure were extended by a comparison of stroke-prone spontaneously hypertensive rats (SP-SHR) with N/nih outbred normotensive rats. Maximal amplitudes of both L-type and T-typ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159064
更新日期：1994-11-01 00:00:00
abstract：:A radioligand binding assay and autoradiographic localisation of P2X-purinoceptors were carried out in several different blood vessels from rat, guinea-pig and rabbit by using [3H]alpha,beta-methylene ATP ([3H]alpha,beta-MeATP) as the radioligand. The radioligand binding assay on rabbit ear artery showed that the bind...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158980
更新日期：1993-03-01 00:00:00
abstract：:The pathophysiology of Raynaud's phenomenon (RP) remains an enigma. Whatever theories proposed, the final event leading to the clinical symptoms is the occlusion of digital vessels. However, the possibility that the upstream large arterial vessels contribute to vasopasm has never been investigated. We used a high reso...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159237
更新日期：1997-07-01 00:00:00
abstract：AIMS:The effects of ischemia and hypercholesterolemia on the function and morphological adaptation of the rabbit hindlimb were assessed. METHODS:In rabbits on normal or cholesterol diet, experiments were performed on days 0-28 following bilateral femoral artery ligation. Calf blood pressure (C(BP)), exercise tolerance...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000113600
更新日期：2008-01-01 00:00:00
abstract：:Signaling pathways activated by the tachykinin substance P (SP) were investigated in pig coronary artery endothelial cells (PCAECs). Single cells were obtained after enzymatic digestion of coronary arteries. Intracellular Ca2+ ([Ca2+]i) was measured from fura-2 fluorescence while membrane potential or ionic current wa...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159222
更新日期：1997-05-01 00:00:00
abstract：:The vascular endothelium is the source of a number of vasodilator and vasoconstrictor autacoids and is thus a key regulator of vascular homeostasis. We studied the effects of altering the balance between protein tyrosine kinase and phosphatase activity on Ca2+ signalling and phosphotyrosine levels in cultured human en...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159150
更新日期：1996-05-01 00:00:00
abstract：BACKGROUND/AIMS:The present report examines a new pig model for progressive induction of high-grade stenosis, for the study of chronic myocardial ischemia and the dynamics of collateral vessel growth. METHODS:Thirty-nine Landrace pigs were instrumented with a novel experimental stent (GVD stent) in the left anterior d...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000181545
更新日期：2009-01-01 00:00:00
abstract：:Proper development of the vascular system as one of the earliest and most critical steps during vertebrate embryogenesis is ensured by the exact spatial and temporal control of gene expression in cells forming the vessel network. Whereas the regulation of vascular system development is well elucidated on the level of ...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000353287
更新日期：2013-01-01 00:00:00
abstract：:Organic nitrates act as vasodilators and have long been used for treatment of cardiovascular diseases. However, the therapeutic effect of nitrates is limited by induction of nitrate tolerance which is associated with endothelial dysfunction and enhanced vasoconstriction. Multiple mechanisms cause nitrate tolerance inc...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000065542
更新日期：2002-07-01 00:00:00
abstract：:This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4 degrees C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2 alp...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159097
更新日期：1995-07-01 00:00:00
abstract：PURPOSE:To determine: (1) whether variables of a pulse wave form analysis of ocular microcirculation shown by laser speckle flowgraphy (LSFG) correlate with left ventricular (LV) systolic dysfunction and (2) whether these variables correlate with LV diastolic dysfunction in subjects without LV systolic dysfunction as a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000494066
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Elevated plasma homocysteine (Hcy) is a risk factor for coronary disease. The objective of this study was to investigate whether Hcy either alone or in high glucose conditions induces endothelin-1 (ET-1) synthesis via the production of reactive oxygen species (ROS). METHODS:Bovine aortic endothelial cells w...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000090947
更新日期：2006-01-01 00:00:00
abstract：:Chemokines play an important role in the process of leukocyte recruitment and activation at sites of inflammation. Until recently, the actions of chemokines and the expression of their receptors have only been described on different leukocyte populations. However, increasing evidence has suggested that non-haematopoie...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000025707
更新日期：2000-01-01 00:00:00
abstract：:The contribution of ATP-sensitive potassium channel (KATP channel) blockade in the vasoconstriction produced by vasopressin was studied. All experiments were performed using rat thoracic aorta cut in 4-mm rings, denuded from their endothelium and mounted into 20-ml organ baths. Glibenclamide (0.01-10 microM), a KATP c...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159088
更新日期：1995-03-01 00:00:00
abstract：:During the early stage of angiogenesis, neovascular sprouts are composed primarily of endothelial cells. As they mature, microvessels acquire a coating of mural cells, which are critical for the development and maintenance of a functional vasculature. Though growth factor regulation of mural cell recruitment has been ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000070711
更新日期：2003-03-01 00:00:00
abstract：:The purpose of the present study was to develop a rapid, reproducible method of nonviral gene transfer to the intact vasculature. Male Sprague-Dawley rats were anesthetized, a midline abdominal incision was made and segmental branches of the superior mesenteric artery were dissected free of surrounding mesentery. A sp...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025753
更新日期：2000-09-01 00:00:00
abstract：:Peroxynitrite is an endothelium-independent vasodilator that induces relaxation via membrane hyperpolarization. The activation of IP3 receptors triggers the opening of potassium channels and hyperpolarization. Previously we found that relaxation to peroxynitrite was maintained during the development of atherosclerosis...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000461581
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND/AIMS:Endothelial colony-forming cells (ECFCs) have the potential to be used in regenerative medicine. Dysfunction of ECFCs is correlated with the onset of cardiovascular disorders, especially coronary artery disease (CAD). Binding of vascular endothelial growth factor A (VEGFA) to vascular endothelial growth...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449202
更新日期：2017-01-01 00:00:00
abstract：:Conduit arteries of hypertensive rats are thicker and stiffer than those of normotensive controls. The possible role played by collagen type I and II subtypes in the mechanism of arterial stiffness remains unknown. The carotid and aortic arterial wall of rats of Japanese (Wistar-Kyoto and spontaneously hypertensive ra...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025637
更新日期：1999-03-01 00:00:00
abstract：UNLABELLED:Diabetic patients have an increased risk of restenosis and late stent thrombosis after angioplasty, i.e. complications that are related to a defective re-endothelialization. Exendin-4, a stable glucagon-like peptide (GLP)-1 receptor agonist, has been suggested to influence the formation of intimal hyperplasi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000381097
更新日期：2015-01-01 00:00:00
abstract：:The novel endothelial cell tyrosine kinase receptors, Tie1 and Tie2, are essential for vascular development and remodeling in the embryo but little is known regarding the regulation of their expression and their role in the maintenance and repair of the adult vascular system. We examined the expression of Tie1 and Tie...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025655
更新日期：1999-07-01 00:00:00
abstract：:The aim of this study was to verify that subcutaneous lymph node transplantation inducing lymphatic regeneration is possible in healthy adult rats, in analogy to results obtained in other species. This rat model was used to determine the effects of lymph node fragmentation as well as sheep erythrocytes and platelet-ri...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000194269
更新日期：2009-01-01 00:00:00
abstract：:Therapies aimed at manipulating the microcirculation require the ability to control angiogenesis, defined as the sprouting of new capillaries from existing vessels. Blocking angiogenesis would be beneficial in many pathologies (e.g. cancer, retinopathies and rheumatoid arthritis). In others (e.g. myocardial infarction...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000362276
更新日期：2014-01-01 00:00:00
abstract：:The localization of dopamine receptor subtypes in the rabbit pulmonary circulation was investigated by using combined radioligand binding and light microscope autoradiography techniques. Dopamine D1-like receptors, probably belonging to the dopamine D1 receptor subtype, were demonstrated in sections of rabbit lung usi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159094
更新日期：1995-05-01 00:00:00
abstract：BACKGROUND/AIMS:Endoplasmic reticulum (ER) stress has emerged as a potential mechanism contributing to diabetes and its comorbidities. However, the importance of ER stress in diabetic vascular dysfunction is unclear. The purpose of this study was to examine the effects of the ER stress inhibitor, tauroursodeoxycholic a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000479967
更新日期：2017-01-01 00:00:00
abstract：:Mechanical forces generated at the endothelium by fluid shear stress and pulsatile stretch are important in ensuring the continuous release of vasoactive endothelial autacoids. Although the mechanism by which endothelial cells are able to detect and convert these physical stimuli into chemical signals is unclear, this...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000025568
更新日期：1998-03-01 00:00:00
