Abstract：
:Previous studies demonstrated that maintenance of steady-state myogenic tone requires Ca(2+)-dependent myosin phosphorylation. The present studies furthered these observations by examining temporal relationships among Ca(2+), myosin phosphorylation and vessel diameter during acute increases in intraluminal pressure and norepinephrine stimulation. Rat cremaster muscle arterioles were cannulated and loaded with the Ca(2+)-sensitive indicator fura-2. The extent of myosin phosphorylation was measured using two-dimensional gel electrophoresis. Acute increases in intraluminal pressure caused a biphasic increase in intracellular Ca(2+) ([Ca(2+)](i)), characterized by a transient peak followed by a decline to a steady-state level which remained significantly higher than control values. Peak [Ca(2+)](i) was significantly related to vessel distension and increased with the change in wall tension. Increased intraluminal pressure resulted in a monophasic increase in myosin phosphorylation that was significantly correlated with instantaneous wall tension. In general, norepinephrine induced larger [Ca(2+)](i) transients and a biphasic myosin phosphorylation pattern. The results demonstrate: (a) major roles for Ca(2+) and myosin phosphorylation in arteriolar myogenic and norepinephrine-induced responses; (b) that changes in Ca(2+) and phosphorylation during a myogenic response are related to changes in wall tension, and (c) differences in Ca(2+) and phosphorylation patterns between the two modes of contraction reflect possible differences in underlying signaling mechanisms. The data further emphasize that spontaneous arteriolar tone represents a state of maintained smooth muscle activation that requires increases in [Ca(2+)](i) and myosin light-chain phosphorylation.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Zou H,Ratz PH,Hill MAdoi
10.1159/000054089keywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
556-67issue
6eissn
1018-1172issn
1423-0135pii
54089journal_volume
37pub_type
杂志文章abstract：:The vascular endothelium is the source of a number of vasodilator and vasoconstrictor autacoids and is thus a key regulator of vascular homeostasis. We studied the effects of altering the balance between protein tyrosine kinase and phosphatase activity on Ca2+ signalling and phosphotyrosine levels in cultured human en...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159150
更新日期：1996-05-01 00:00:00
abstract：:Mechanical forces generated at the endothelium by fluid shear stress and pulsatile stretch are important in ensuring the continuous release of vasoactive endothelial autacoids. Although the mechanism by which endothelial cells are able to detect and convert these physical stimuli into chemical signals is unclear, this...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000025568
更新日期：1998-03-01 00:00:00
abstract：:In the present study, we investigated the actions of calmodulin (CaM) and CaM-dependent protein kinase II (CaMK-II) on the L-type Ca2+ currents (ICa(L)) of cultured vascular smooth muscle (VSM) cells (A7r5 cell line), using the whole-cell voltage clamp method. The peak IBa (Ca2+ channel using 5 mM Ba2+ as charge carri...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025599
更新日期：1998-09-01 00:00:00
abstract：:Thrombospondin-1 (TSP1) is a potent natural inhibitor of angiogenesis. Although TSP1 has been reported to induce endothelial cell apoptosis in vitro and to downregulate neovascularization in vivo, the molecular mechanisms that link these two processes have yet to be established. Here we report that TSP1 mediates endot...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025733
更新日期：2000-05-01 00:00:00
abstract：:Studies on the possible causal relationship between the Ca2+ channel current density in the vascular muscle cell (VMC) and increases in blood pressure were extended by a comparison of stroke-prone spontaneously hypertensive rats (SP-SHR) with N/nih outbred normotensive rats. Maximal amplitudes of both L-type and T-typ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159064
更新日期：1994-11-01 00:00:00
abstract：BACKGROUND:Controversial reports on prolactin receptors (PRL-R), the long and short form, on endothelial cells (EC) may be explained by the choice of EC derived from the micro- and macrovascular bed of either endocrine and non-endocrine organs. METHODS:We studied here PRL-R expression in organs [bovine corpus luteum (...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000097892
更新日期：2007-01-01 00:00:00
abstract：:Ruptured atherosclerotic plaques, lined with activated platelets, constitute an attractive target for magnetic resonance imaging (MRI). This study evaluated whether microparticles of iron oxide (MPIO) targeting ligand-induced binding sites (LIBS) on the activated conformation of glycoprotein IIb/IIIa could be used to ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000135660
更新日期：2009-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000074550
更新日期：2003-09-01 00:00:00
abstract：:The purpose of this study was to determine the contribution of long-chain fatty acids relative to other mitochondrial substrates in active vascular smooth muscle (VSM). Hog carotid arteries were isometrically contracted in physiological saline solution containing 0.71 mM U-(13)C-oleic acid (bound to albumin at a ratio...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051056
更新日期：2001-05-01 00:00:00
abstract：:Actin cytoskeleton reorganization is regulated by various actin-binding proteins. Cofilin is the principal filament-depolymerizing protein, whose activity is reduced upon phosphorylation by LIMK. Thus, LIMK and cofilin comprise a signal transduction module regulating actin turnover and myogenic tone in healthy vascula...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000081247
更新日期：2004-09-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000258905
更新日期：2010-01-01 00:00:00
abstract：:The Notch4 signaling pathway of endothelial progenitor cells (EPCs) may play a crucial role in Kawasaki disease (KD). We investigated the proliferation, adhesion, migration, angiogenesis, and expression levels of Notch4, recombination signal-binding protein-Jκ (RBP-Jκ), P-selectin, and vascular cell adhesion molecule-...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449061
更新日期：2016-01-01 00:00:00
abstract：:Fibroblast growth factor-2 (FGF2) and vascular endothelial growth factor (VEGF) exert their angiogenic activity by interacting with endothelial cells in a distinct manner. In this study, we investigated the morphological features of endothelial cells of the chick embryo chorioallantoic membrane (CAM) microvasculature ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051070
更新日期：2001-07-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159205
更新日期：1997-03-01 00:00:00
abstract：:Previous studies have demonstrated endothelial and smooth muscle hyperplasia occur during arterial luminal expansion associated with elevation of arterial wall shear rates. The current study investigated whether remodeling induced by elevated wall shear would ultimately result in a vessel with intimal and medial cell ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025592
更新日期：1998-07-01 00:00:00
abstract：:Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159106
更新日期：1995-09-01 00:00:00
abstract：OBJECTIVES:Hyperuricemia provokes endothelial dysfunction (ECD). Decreased endothelial nitric oxide synthase (eNOS) activity is an important source of ECD. Cationic amino acid transporter-1 (CAT-1) is the specific arginine transporter for eNOS. We hypothesize that hyperuricemia inhibits arginine uptake. METHODS:Experi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000320356
更新日期：2011-01-01 00:00:00
abstract：:In coronary artery diseases, cigarette smoking is a risk factor and the endothelin system plays a key role in the pathogenesis. This study was to examine if dimethylsulfoxide-soluble smoke particles (DSP) upregulate endothelin type-B (ETB) receptors in the coronary artery and investigate the mechanism. The isolated ra...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000350866
更新日期：2013-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000447263
更新日期：2016-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000101777
更新日期：2007-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159088
更新日期：1995-03-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025580
更新日期：1998-05-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000071891
更新日期：2003-05-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025637
更新日期：1999-03-01 00:00:00
abstract：:A radioligand binding assay and autoradiographic localisation of P2X-purinoceptors were carried out in several different blood vessels from rat, guinea-pig and rabbit by using [3H]alpha,beta-methylene ATP ([3H]alpha,beta-MeATP) as the radioligand. The radioligand binding assay on rabbit ear artery showed that the bind...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158980
更新日期：1993-03-01 00:00:00
abstract：BACKGROUND:Transfer of molecular signals from veins to adjacent arteries is an established mechanism of vascular communication in the uterine circulation, which ultimately depends on venous permeability. This study tests the hypotheses that: (1) uterine veins are permeable to intravenous solutes, using a 3-kDa dextran ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000082976
更新日期：2005-01-01 00:00:00
abstract：:Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass g...
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pub_type： 杂志文章
doi：10.1159/000376548
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND/AIMS:The endothelium has been recognized as a key component in the regulation of blood vessels. We designed a simple procedure to separate endothelial and smooth muscle RNA from rat aorta and mesenteric artery and used this method to establish the distribution of Na(+)/K(+)-ATPase alpha-subunit isoforms (NaK...
journal_title：Journal of vascular research
pub_type： 杂志文章
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更新日期：2006-01-01 00:00:00
abstract：BACKGROUND/AIMS:Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress in resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of the membrane-bound enzyme to Golgi ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000355301
更新日期：2013-01-01 00:00:00
abstract：:Although the content of histamine in myocardial tissue is high, its contribution to the regulation of coronary blood flow has not been clearly defined. The aim of the present study was to investigate whether or not nitric oxide (NO), an important modulator of coronary vascular tone, is involved in histamine-induced co...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158987
更新日期：1993-05-01 00:00:00
