Abstract:
:Topical application of elastase to induce arterial aneurysm formation is an emerging murine model of vascular disease. In the context of aortic abdominal aneurysm (AAA), angiotensin II infusion and porcine pancreatic elastase perfusion models are commonly used today. This study, therefore, compares matrix remodeling, inflammation, and angiogenesis as distinct features of aneurysms in two models treated with intra-/extraluminal elastase. C57BL/6 mice underwent intra-/extraluminal elastase application via laparotomy and were followed up for 4 weeks. Basic histology and immunohistochemistry were performed at different time points along with transmission electron microscopy, PCR analysis, TUNEL assays, and blood analysis. Both models did not differ in aneurysm growth rate, but they showed distinct features and results depending on the way of elastase application. Extraluminal aneurysm induction preserved endothelial cell function and elastic fibers but showed ongoing acute inflammation, mainly in the adventitia. The destruction of elastic layers followed by chronic inflammation was a characteristic of intraluminal elastase perfusion, as well as medial angiogenesis, a key feature in human AAA. Different animal models harbor different features of human AAA and must, therefore, be chosen wisely. External elastase application mimics an acute inflammatory aneurysm, whereas intraluminal elastase perfusion shows chronic inflammation with angiogenesis and endothelial destruction, thus better mimicking human disease.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Busch A,Holm A,Wagner N,Ergün S,Rosenfeld M,Otto C,Baur J,Kellersmann R,Lorenz Udoi
10.1159/000447263subject
Has Abstractpub_date
2016-01-01 00:00:00pages
49-57issue
1-2eissn
1018-1172issn
1423-0135pii
000447263journal_volume
53pub_type
杂志文章abstract:BACKGROUND:A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smoot...
journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000318777
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:To determine: (1) whether variables of a pulse wave form analysis of ocular microcirculation shown by laser speckle flowgraphy (LSFG) correlate with left ventricular (LV) systolic dysfunction and (2) whether these variables correlate with LV diastolic dysfunction in subjects without LV systolic dysfunction as a...
journal_title:Journal of vascular research
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000158963
更新日期:1992-11-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159113
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pub_type: 杂志文章
doi:10.1159/000135660
更新日期:2009-01-01 00:00:00
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journal_title:Journal of vascular research
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doi:10.1159/000082976
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025733
更新日期:2000-05-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000124283
更新日期:2008-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025616
更新日期:1998-11-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000153247
更新日期:2009-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000481781
更新日期:2017-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159077
更新日期:1995-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025580
更新日期:1998-05-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000113600
更新日期:2008-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449059
更新日期:2016-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000449202
更新日期:2017-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000448713
更新日期:2016-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000025559
更新日期:1998-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
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更新日期:2016-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章,评审
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更新日期:2014-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000101450
更新日期:2007-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000085721
更新日期:2005-05-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000097892
更新日期:2007-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000156704
更新日期:2009-01-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000159237
更新日期:1997-07-01 00:00:00
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journal_title:Journal of vascular research
pub_type: 杂志文章
doi:10.1159/000109818
更新日期:2008-01-01 00:00:00