Protective Function of Ahnak1 in Vascular Healing after Wire Injury.


AIM:Vascular remodeling following injury substantially accounts for restenosis and adverse clinical outcomes. In this study, we investigated the role of the giant scaffold protein Ahnak1 in vascular healing after endothelial denudation of the murine femoral artery. METHODS:The spatiotemporal expression pattern of Ahnak1 and Ahnak2 was examined using specific antibodies and real-time quantitative PCR. Following wire-mediated endothelial injury of Ahnak1-deficient mice and wild-type (WT) littermates, the processes of vascular healing were analyzed. RESULTS:Ahnak1 and Ahnak2 showed a mutually exclusive vascular expression pattern, with Ahnak1 being expressed in the endothelium and Ahnak2 in the medial cells in naïve WT arteries. After injury, a marked increase of Ahnak1- and Ahnak2-positive cells at the lesion site became evident. Both proteins showed a strong upregulation in neointimal cells 14 days after injury. Ahnak1-deficient mice showed delayed vascular healing and dramatically impaired re-endothelialization that resulted in prolonged adverse vascular remodeling, when compared to the WT littermates. CONCLUSION:The large scaffold and adaptor proteins Ahnak1 and Ahnak2 exhibit differential expression patterns and functions in naïve and injured arteries. Ahnak1 plays a nonredundant protective role in vascular healing.


J Vasc Res


Haase N,Rüder C,Haase H,Kamann S,Kouno M,Morano I,Dechend R,Zohlnhöfer D,Haase T




Has Abstract


2017-01-01 00:00:00














  • Inhibition of vascular smooth muscle cell migration by intact endothelium is nitric oxide-mediated: interference by oxidised low density lipoproteins.

    abstract::Migration of vascular smooth muscle cells (SMCs) leading to neointimal hyperplasia is an early and cardinal feature of atherogenesis. Migration of rat aortic SMCs from an upper chamber towards a lower one has been studied in a microchemotaxis (Boyden) chamber. Spontaneous migration of SMCs was practically prevented by...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Gorog P,Kovacs IB

    更新日期:1998-05-01 00:00:00

  • Blood outgrowth endothelial cells reduce hypoxia-mediated fibroblast to myofibroblast conversion by decreasing proangiogenic cytokines.

    abstract:PURPOSE:Hypoxic conditions cause fibroblasts to differentiate into alpha smooth-muscle cell actin (α -SMA)-positive cells, i.e. myofibroblasts. This process is a hallmark of venous neointimal hyperplasia (VNH) associated with hemodialysis vascular access. The purpose of this study was to determine if blood outgrowth en...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Nieves Torres EC,Yang B,Brahmbhatt A,Mukhopadhyay D,Misra S

    更新日期:2014-01-01 00:00:00

  • Long-term sensory denervation does not modify endothelial function or endothelial substance P and nitric oxide synthase in rat mesenteric arteries.

    abstract::Mesenteric endothelial cell function and immunoreactivity for substance P and nitric oxide synthase (NOS) were examined in control rats and rats treated with capsaicin as neonates to destroy primary afferent nerves. Endothelial vasodilator function was examined pharmacologically in the methoxamine raised-tone isolated...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Ralevic V,Dikranian K,Burnstock G

    更新日期:1995-09-01 00:00:00

  • Structural heterogeneity of the diffuse intimal thickening and correlation with distribution of TGF-beta 1.

    abstract::This study examines the ultrastructural features of diffuse intimal thickenings of human coronary arteries and correlates structural heterogeneity along the radial axis with the distribution and known actions of transforming growth factor beta 1 (TGF-beta 1). Morphometric and immunohistochemical data were collected fr...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Merrilees MJ,Beaumont B

    更新日期:1993-09-01 00:00:00

  • The Cytoscan Model E-II, a new reflectance microscope for intravital microscopy: comparison with the standard fluorescence method.

    abstract::The Cytoscan Model E-II (Cytometrics Inc., Philadelphia, Pa., USA) is a newly developed instrument which functions as an intravital microscope and is small and easily portable. Through the use of orthogonal polarization spectral (OPS) imaging, the Cytoscan Model E-II delivers images of the microcirculation which are c...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Harris AG,Sinitsina I,Messmer K

    更新日期:2000-11-01 00:00:00

  • Endothelial modulation of ouabain-induced contraction and sodium pump activity in aortas of normotensive Wistar-Kyoto and spontaneously hypertensive rats.

    abstract::The influence of vascular endothelium on ouabain-induced contractions and sodium pump activity in aortic segments of Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) was analyzed. De-endothelialization increased and reduced ouabain-induced contractions in WKY and SHR segments, respectively. The effects of d...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Ponte A,Marín J,Arribas S,González R,Barrús MT,Salaices M,Sánchez-Ferrer CF

    更新日期:1996-03-01 00:00:00

  • Distribution and peptide content of sympathetic axons innervating different regions of the cutaneous venous bed in the pinna of the guinea pig ear.

    abstract::This study utilised catecholamine fluorescence histochemistry, multiple-labelling immunohistochemistry and retrograde axonal transport to determine the distribution and neuropeptide content of sympathetic neurons innervating veins in the dorsal skin of the guinea pig pinna. There was a dense plexus of sympathetic axon...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Morris JL

    更新日期:1995-11-01 00:00:00

  • Oxygen-dependent vasomotor responses are conducted upstream in the mouse cremaster microcirculation.

    abstract:BACKGROUND:A new method was evaluated where local changes in oxygen tension were induced in a tissue while being studied under a microscope in vivo. We tested whether hypoxic vasodilation and hyperoxic vasoconstriction in arterioles in striated muscle are being propagated upstream, and whether the endothelium and smoot...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Riemann M,Rai A,Ngo AT,Dziegiel MH,Holstein-Rathlou NH,Torp-Pedersen C

    更新日期:2011-01-01 00:00:00

  • Pulse Waveform Analysis in Ocular Microcirculation by Laser Speckle Flowgraphy in Patients with Left Ventricular Systolic and Diastolic Dysfunction.

    abstract:PURPOSE:To determine: (1) whether variables of a pulse wave form analysis of ocular microcirculation shown by laser speckle flowgraphy (LSFG) correlate with left ventricular (LV) systolic dysfunction and (2) whether these variables correlate with LV diastolic dysfunction in subjects without LV systolic dysfunction as a...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Shiba T,Takahashi M,Matsumoto T,Hori Y

    更新日期:2018-01-01 00:00:00

  • Interaction between nitric oxide and cyclooxygenase pathways in endothelial cells.

    abstract:UNLABELLED:Nitric oxide (NO) and cyclooxygenase (COX) derived prostaglandins (PGs) are involved in vascular homeostasis. Contradictory results have been obtained in previous studies on the putative 'cross-talk' between these two pathways. Our aim was to evaluate the interaction between NO and PG release in human microv...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Vassalle C,Domenici C,Lubrano V,L'Abbate A

    更新日期:2003-09-01 00:00:00

  • Morphogenic effects of endothelin-1 on vascular smooth muscle cells.

    abstract::Endothelin-1 (ET-1), a vasoconstrictor peptide produced by endothelial and vascular smooth muscle cells (VSMC) might play a role in vascular remodelling. To investigate the proposed 'mitogenic' potential of ET-1, we examined the effects of chronic exposure of VSMC to ET-1 on cell cycle, growth/proliferation and differ...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Hahn AW,Regenass S,Resink TJ,Kern F,Bühler FR

    更新日期:1993-07-01 00:00:00

  • Focused in vivo delivery of plasmid DNA to the porcine vascular wall via intravascular ultrasound destruction of microbubbles.

    abstract:BACKGROUND:Safety concerns associated with drug-eluting stents have spurred interest in alternative vessel therapeutics following angioplasty. Microbubble contrast agents have been shown to increase gene transfection in vivo in the presence of ultrasound. OBJECTIVES/METHODS:The purpose of this study was to determine w...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Phillips LC,Klibanov AL,Bowles DK,Ragosta M,Hossack JA,Wamhoff BR

    更新日期:2010-01-01 00:00:00

  • Evaluation of Biodegradable Stent Graft Coatings in Pig and Rabbit Models.

    abstract:AIMS:Percutaneous coronary intervention is routinely performed to treat occlusive coronary artery disease. Coronary perforation is a potential complication and can be treated with a stent graft. Current stent grafts are associated with high restenosis rates. We tested the safety and feasibility of biodegradable stent g...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Taavitsainen J,Tarvainen S,Kuivanen A,Mangiardi EK,Guelcher M,Martin J,Mathur A,Hytönen JP,Ylä-Herttuala S

    更新日期:2020-01-01 00:00:00

  • Direct Evidence for P2Y2 Receptor Involvement in Vascular Response to Injury.

    abstract:OBJECTIVES:Extracellular nucleotide release at the site of arterial injury mediates the proliferation and migration of vascular smooth muscle cells. Our aim was to investigate the role of the P2Y2 nucleotide receptor (P2Y2R) in neointimal hyperplasia. Approach and Results: Vascular injury was induced by the implantatio...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Agca Y,Qian S,Agca C,Seye CI

    更新日期:2016-01-01 00:00:00

  • Differential Regulation of BMAL1, CLOCK, and Endothelial Signaling in the Aortic Arch and Ligated Common Carotid Artery.

    abstract::The circadian clock is rhythmically expressed in blood vessels, but the interaction between the circadian clock and disturbed blood flow remains unclear. We examined the relationships between BMAL1 and CLOCK and 2 regulators of endothelial function, AKT1 and endothelial nitric oxide synthase (eNOS), in vascular region...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Shang X,Pati P,Anea CB,Fulton DJ,Rudic RD

    更新日期:2016-01-01 00:00:00

  • Wall remodeling after wall shear rate normalization in rat mesenteric arterial collaterals.

    abstract::Previous studies have demonstrated endothelial and smooth muscle hyperplasia occur during arterial luminal expansion associated with elevation of arterial wall shear rates. The current study investigated whether remodeling induced by elevated wall shear would ultimately result in a vessel with intimal and medial cell ...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Fath SW,Burkhart HM,Miller SC,Dalsing MC,Unthank JL

    更新日期:1998-07-01 00:00:00

  • Cardiovascular protective effects of synthetic isoflavone derivatives in apolipoprotein e-deficient mice.

    abstract::Dietary flavonoids are thought to protect against cardiovascular disease. We have studied the effects of bioactive isoflavone metabolites on hyperlipidemia, endothelial dysfunction and the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE(0)) mice fed a Western high-fat diet. Supplementation w...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Jiang F,Jones GT,Husband AJ,Dusting GJ

    更新日期:2003-05-01 00:00:00

  • Smooth muscle cells influence monocyte response to LDL as well as their adhesion and transmigration in a coculture model of the arterial wall.

    abstract::We investigated the possible interference of smooth muscle cells with monocyte response to LDL as well as with their adhesion and transmigration in a coculture of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to e...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Kinard F,Jaworski K,Sergent-Engelen T,Goldstein D,Van Veldhoven PP,Holvoet P,Trouet A,Schneider YJ,Remacle C

    更新日期:2001-09-01 00:00:00

  • Changes in adrenoceptors and G-protein-coupled receptor kinase 2 in L-NAME-induced hypertension compared to spontaneous hypertension in rats.

    abstract::This work compares the expression of adrenoceptors (ARs) and G-protein-coupled receptor kinase (GRK) 2 (RT-PCR and immunoblotting) and functional responses in conductance (aorta) and resistance vessels (mesenteric resistance arteries; MRA) in two different models of rat hypertension: hypertension induced by chronic tr...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Oliver E,Flacco N,Arce C,Ivorra MD,D'Ocon MP,Noguera MA

    更新日期:2014-01-01 00:00:00

  • Lipopolysaccharide-induced cytokine and chemokine expression in human carotid lesions.

    abstract::The release of cytokines and chemokines from activated immune-competent cells plays a crucial role in determining the pathology of the atherogenic progress. We investigated the effect of bacterial lipopolysaccharide (LPS) on cytokine/chemokine expression in carotid lesions and normal renal arteries. The lesions or ren...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Jatta K,Wågsäter D,Norgren L,Stenberg B,Sirsjö A

    更新日期:2005-05-01 00:00:00

  • Anisodamine counteracts lipopolysaccharide-induced tissue factor and plasminogen activator inhibitor-1 expression in human endothelial cells: contribution of the NF-kappa b pathway.

    abstract::In this study we aimed to investigate whether the therapeutic efficacy of anisodamine in the treatment of bacteraemic shock could--at least in part--be brought about by its direct interference with the lipopolysaccharide (LPS)-induced activation of endothelial cells. Thus, we investigated the effect of anisodamine on ...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Ruan QR,Zhang WJ,Hufnagl P,Kaun C,Binder BR,Wojta J

    更新日期:2001-01-01 00:00:00

  • Tyrosine phosphorylation and regulation of swine carotid artery contraction.

    abstract::Regulation of [Ca2+]i and modulation of the [Ca2+]i sensitivity of myosin phosphorylation in smooth muscles may involve phosphorylation of one or more proteins on tyrosine residues. We tested this hypothesis by measuring tyrosine phosphorylation of proteins extracted from swine carotid artery and separated by SDS gel ...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Rembold CM,Weaver BA

    更新日期:1997-01-01 00:00:00

  • Human vascular reactivity and polymorphisms of the angiotensin-converting enzyme and the angiotensin type 1 receptor genes.

    abstract::The D allele of the insertion (I)/deletion (D) polymorphism in the angiotensin-converting enzyme (ACE) gene and the C allele of the A1166-C polymorphism in the angiotensin II type 1 receptor (AGT1R) gene have been associated with altered vascular structure and with an increased risk of myocardial infarction. The aim o...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Steeds RP,Toole LO,Channer KS,Morice AH

    更新日期:1999-11-01 00:00:00

  • PDGF and IL-1beta upregulate cofilin and LIMK2 in canine cultured pulmonary artery smooth muscle cells.

    abstract::Actin cytoskeleton reorganization is regulated by various actin-binding proteins. Cofilin is the principal filament-depolymerizing protein, whose activity is reduced upon phosphorylation by LIMK. Thus, LIMK and cofilin comprise a signal transduction module regulating actin turnover and myogenic tone in healthy vascula...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Bongalon S,Dai YP,Singer CA,Yamboliev IA

    更新日期:2004-09-01 00:00:00

  • Developmental biology of the vascular smooth muscle cell: building a multilayered vessel wall.

    abstract::The assembly of the vessel wall from its cellular and extracellular matrix components is a critical process in the development and maturation of the cardiovascular system. However, fundamental questions concerning the origin of vessel wall cells and the mechanisms that regulate their development and differentiation re...

    journal_title:Journal of vascular research

    pub_type: 杂志文章,评审


    authors: Hungerford JE,Little CD

    更新日期:1999-01-01 00:00:00

  • Transcriptional down-regulation of thromboxane A(2) receptor expression via activation of MAPK ERK1/2, p38/NF-kappaB pathways.

    abstract:BACKGROUND:We have developed an in vitro model by organ culture of rat mesenteric arteries to imitate vascular smooth muscle cell (VSMC) receptor changes in cardiovascular disease. By using this model, alteration of VSMC thromboxane A(2) (TP) receptors was studied. METHODS AND RESULTS:After organ culture of the arteri...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Zhang W,Zhang Y,Edvinsson L,Xu CB

    更新日期:2009-01-01 00:00:00

  • Calcium signalling and autacoid production in endothelial cells are modulated by changes in tyrosine kinase and phosphatase activity.

    abstract::The vascular endothelium is the source of a number of vasodilator and vasoconstrictor autacoids and is thus a key regulator of vascular homeostasis. We studied the effects of altering the balance between protein tyrosine kinase and phosphatase activity on Ca2+ signalling and phosphotyrosine levels in cultured human en...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Fleming I,Bara AT,Busse R

    更新日期:1996-05-01 00:00:00

  • Differential distribution of mechanosensitive nonselective cation channels in systemic and pulmonary arterial myocytes of rabbits.

    abstract::The mechanosensitive nonselective cation channel (NSC(MS)) is a key player in vascular myogenic contraction. The functional channel density and pressure sensitivity of NSC(MS) in vascular myocytes were compared between pulmonary and systemic arteries (coronary, mesenteric and cerebral arteries) in the rabbit. In cell-...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Park KS,Lee HA,Earm KH,Ko JH,Earm YE,Kim SJ

    更新日期:2006-01-01 00:00:00

  • Expression of IL-1beta, IL-1 receptor type I and IL-1 receptor antagonist in human aortic smooth muscle cells: effects of all-trans-retinoic acid.

    abstract::The proinflammatory cytokine interleukin (IL)-1beta and the IL-1 receptor antagonist are expressed by atherosclerotic plaques and may be linked to the development of atherosclerosis. Existing evidence shows that retinoids and their receptors are involved in inflammatory response and that they are found in atherosclero...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Wågsater D,Jatta K,Ocaya P,Dimberg J,Sirsjo A

    更新日期:2006-01-01 00:00:00

  • Diminished insulin-mediated forearm blood flow and muscle glucose uptake in young men with low birth weight.

    abstract:BACKGROUND:Low birth weight (LBW) is associated with increased risk of type 2 diabetes and cardiovascular disease. We studied endothelial function and insulin sensitivity in young men with LBW (n = 22) and controls (n = 22). METHODS:Insulin sensitivity and endothelial function was studied with venous occlusion plethys...

    journal_title:Journal of vascular research

    pub_type: 杂志文章


    authors: Sonne MP,Højbjerre L,Alibegovic AC,Vaag A,Stallknecht B,Dela F

    更新日期:2010-01-01 00:00:00