Abstract：
BACKGROUND/AIMS:Endoplasmic reticulum (ER) stress has emerged as a potential mechanism contributing to diabetes and its comorbidities. However, the importance of ER stress in diabetic vascular dysfunction is unclear. The purpose of this study was to examine the effects of the ER stress inhibitor, tauroursodeoxycholic acid (TUDCA), on arterial stiffness and endothelial dysfunction in type 2 diabetic mice. METHODS:Carotid and mesenteric artery endothelial function were assessed via ex vivo pressure myography, and arterial stiffness was measured by aortic pulse wave velocity. The effects of TUDCA were examined both acutely (ex vivo) and chronically (250 mg/kg/day; i.p., 4 weeks). RESULTS:Compared to control C57BL/6J mice, db/db (DB) mice did not display carotid artery endothelial dysfunction; however, mesenteric artery endothelial function was markedly impaired. Acute incubation and chronic administration of TUDCA improved endothelium-dependent dilation in DB mesenteric arteries, without affecting endothelium-independent dilation. Chronic TUDCA administration also reduced arterial stiffness and was associated with reductions in ER stress markers in aortic and perivascular adipose tissue. CONCLUSIONS:These results suggest that ER stress may represent a novel cause of, and therapeutic target for, diabetic vascular dysfunction.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Battson ML,Lee DM,Jarrell DK,Hou S,Ecton KE,Phan AB,Gentile CLdoi
10.1159/000479967subject
Has Abstractpub_date
2017-01-01 00:00:00pages
280-287issue
5eissn
1018-1172issn
1423-0135pii
000479967journal_volume
54pub_type
杂志文章abstract：:Cx40-deficient mice (Cx40-/-) are hypertensive due to increased renin secretion. We evaluated the renal expression of neuronal nitric oxide synthase (nNOS) and cyclooxygenases COX-1 and COX-2, three macula densa enzymes. The levels of nNOS were increased in kidneys of Cx40-/- mice, as well as in those of wild-type (WT...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000156704
更新日期：2009-01-01 00:00:00
abstract：:The influence of vascular endothelium on ouabain-induced contractions and sodium pump activity in aortic segments of Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) was analyzed. De-endothelialization increased and reduced ouabain-induced contractions in WKY and SHR segments, respectively. The effects of d...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159145
更新日期：1996-03-01 00:00:00
abstract：:Proper development of the vascular system as one of the earliest and most critical steps during vertebrate embryogenesis is ensured by the exact spatial and temporal control of gene expression in cells forming the vessel network. Whereas the regulation of vascular system development is well elucidated on the level of ...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000353287
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND/AIMS:The present report examines a new pig model for progressive induction of high-grade stenosis, for the study of chronic myocardial ischemia and the dynamics of collateral vessel growth. METHODS:Thirty-nine Landrace pigs were instrumented with a novel experimental stent (GVD stent) in the left anterior d...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000181545
更新日期：2009-01-01 00:00:00
abstract：:The pathophysiology of Raynaud's phenomenon (RP) remains an enigma. Whatever theories proposed, the final event leading to the clinical symptoms is the occlusion of digital vessels. However, the possibility that the upstream large arterial vessels contribute to vasopasm has never been investigated. We used a high reso...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159237
更新日期：1997-07-01 00:00:00
abstract：:It has been well established that wall shear stress is an important determinant of endothelial cell function and gene expression as well as of its structure. There is increasing evidence that low wall shear stress, as present in artery bifurcations opposite to the flow divider where atherosclerotic lesions preferentia...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000091648
更新日期：2006-01-01 00:00:00
abstract：:Migration of vascular smooth muscle cells (SMCs) leading to neointimal hyperplasia is an early and cardinal feature of atherogenesis. Migration of rat aortic SMCs from an upper chamber towards a lower one has been studied in a microchemotaxis (Boyden) chamber. Spontaneous migration of SMCs was practically prevented by...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025580
更新日期：1998-05-01 00:00:00
abstract：:Dietary flavonoids are thought to protect against cardiovascular disease. We have studied the effects of bioactive isoflavone metabolites on hyperlipidemia, endothelial dysfunction and the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE(0)) mice fed a Western high-fat diet. Supplementation w...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000071891
更新日期：2003-05-01 00:00:00
abstract：:The effect of insulin on the vasoconstriction induced by norepinephrine is presently controversial. Therefore, the aims of our study were: (1) to evaluate the effect of low- and high-dose insulin on the concentration-response curve to norepinephrine in small resistance arteries of spontaneously hypertensive rats (SHR)...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025679
更新日期：1999-09-01 00:00:00
abstract：BACKGROUND:Hypoxia results in pulmonary hypertension and vascular remodeling due to induction of pulmonary artery cell proliferation. Besides pulmonary artery smooth muscle cells, pulmonary artery endothelial cells (PAECs) are also involved in the development of pulmonary hypertension, but the effect of hypoxia on PAEC...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000327005
更新日期：2011-01-01 00:00:00
abstract：:The vascular endothelium is the source of a number of vasodilator and vasoconstrictor autacoids and is thus a key regulator of vascular homeostasis. We studied the effects of altering the balance between protein tyrosine kinase and phosphatase activity on Ca2+ signalling and phosphotyrosine levels in cultured human en...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159150
更新日期：1996-05-01 00:00:00
abstract：:Atherosclerosis underlies late occlusion of human saphenous vein (HSV) coronary artery bypass grafts. Monocyte infiltration is implicated, but its mechanisms are unclear given that HSV normally expresses the ICAM-1 but not the VCAM-1 adhesion molecule. To define the mechanisms underlying monocyte adhesion, samples of ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000063687
更新日期：2002-05-01 00:00:00
abstract：:Increased vascular disease occurs with low albumin (human serum albumin, HSA), possibly reflecting specific inhibition of endothelial apoptosis reported for tissue culture. Despite the reported specificity for endothelial protection by HSA, the high but physiological concentrations needed appear more consistent with n...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000101777
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND:Elevated plasma homocysteine (Hcy) is a risk factor for coronary disease. The objective of this study was to investigate whether Hcy either alone or in high glucose conditions induces endothelin-1 (ET-1) synthesis via the production of reactive oxygen species (ROS). METHODS:Bovine aortic endothelial cells w...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000090947
更新日期：2006-01-01 00:00:00
abstract：:Regulation of [Ca2+]i and modulation of the [Ca2+]i sensitivity of myosin phosphorylation in smooth muscles may involve phosphorylation of one or more proteins on tyrosine residues. We tested this hypothesis by measuring tyrosine phosphorylation of proteins extracted from swine carotid artery and separated by SDS gel ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159196
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND/AIMS:Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress in resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of the membrane-bound enzyme to Golgi ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000355301
更新日期：2013-01-01 00:00:00
abstract：:Conduit arteries of hypertensive rats are thicker and stiffer than those of normotensive controls. The possible role played by collagen type I and II subtypes in the mechanism of arterial stiffness remains unknown. The carotid and aortic arterial wall of rats of Japanese (Wistar-Kyoto and spontaneously hypertensive ra...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025637
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND/AIMS:Endothelial colony-forming cells (ECFCs) have the potential to be used in regenerative medicine. Dysfunction of ECFCs is correlated with the onset of cardiovascular disorders, especially coronary artery disease (CAD). Binding of vascular endothelial growth factor A (VEGFA) to vascular endothelial growth...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449202
更新日期：2017-01-01 00:00:00
abstract：:Inflammation is a highly regulated process with common but also specific characteristics in each tissue affected. Recruitment of leukocytes from the blood to the injured tissue is an important early step in the inflammatory cascade. This review highlights the role of connexins (Cxs) in the regulation of both acute and...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000316942
更新日期：2011-01-01 00:00:00
abstract：:Pituitary adenylate cyclase-activating peptide (PACAP; 1-38 and 1-27) and vasoactive intestinal peptide (VIP) are related neuropeptides of the secretin/glucagon family. Overlapping signaling through G-protein-coupled receptors mediates their vasomotor activity. We previously showed that PACAP deficiency (PACAP-KO) shi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000481781
更新日期：2017-01-01 00:00:00
abstract：AIMS:Percutaneous coronary intervention is routinely performed to treat occlusive coronary artery disease. Coronary perforation is a potential complication and can be treated with a stent graft. Current stent grafts are associated with high restenosis rates. We tested the safety and feasibility of biodegradable stent g...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000505454
更新日期：2020-01-01 00:00:00
abstract：:We investigated the possible interference of smooth muscle cells with monocyte response to LDL as well as with their adhesion and transmigration in a coculture of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to e...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051081
更新日期：2001-09-01 00:00:00
abstract：:This study examines the ultrastructural features of diffuse intimal thickenings of human coronary arteries and correlates structural heterogeneity along the radial axis with the distribution and known actions of transforming growth factor beta 1 (TGF-beta 1). Morphometric and immunohistochemical data were collected fr...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159008
更新日期：1993-09-01 00:00:00
abstract：:Intima-media thickening (IMT) of the carotid artery, a form of vascular remodeling, correlates well with coronary artery disease risk in humans. Vascular remodeling in response to blood flow is a complex process that critically involves altered cell matrix interactions. To gain insight into these events, we performed ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000081804
更新日期：2004-11-01 00:00:00
abstract：:The contribution of ATP-sensitive potassium channel (KATP channel) blockade in the vasoconstriction produced by vasopressin was studied. All experiments were performed using rat thoracic aorta cut in 4-mm rings, denuded from their endothelium and mounted into 20-ml organ baths. Glibenclamide (0.01-10 microM), a KATP c...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159088
更新日期：1995-03-01 00:00:00
abstract：:The novel endothelial cell tyrosine kinase receptors, Tie1 and Tie2, are essential for vascular development and remodeling in the embryo but little is known regarding the regulation of their expression and their role in the maintenance and repair of the adult vascular system. We examined the expression of Tie1 and Tie...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025655
更新日期：1999-07-01 00:00:00
abstract：:Although the content of histamine in myocardial tissue is high, its contribution to the regulation of coronary blood flow has not been clearly defined. The aim of the present study was to investigate whether or not nitric oxide (NO), an important modulator of coronary vascular tone, is involved in histamine-induced co...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158987
更新日期：1993-05-01 00:00:00
abstract：:This study utilised catecholamine fluorescence histochemistry, multiple-labelling immunohistochemistry and retrograde axonal transport to determine the distribution and neuropeptide content of sympathetic neurons innervating veins in the dorsal skin of the guinea pig pinna. There was a dense plexus of sympathetic axon...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159113
更新日期：1995-11-01 00:00:00
abstract：BACKGROUND:Angiopoietin-like protein 3 (ANGPTL3) is a liver-derived plasma protein that modulates plasma triglyceride clearance, angiogenesis and atherosclerosis in experimental models. So far, no study has examined its role in atherosclerosis in human subjects. We evaluated the possible association between plasma ANGP...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000098153
更新日期：2007-01-01 00:00:00
abstract：:This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4 degrees C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2 alp...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159097
更新日期：1995-07-01 00:00:00
