Abstract：
:beta-Amyloid is the primary protein component of neuritic plaques, which are degenerative foci in brains of patients with Alzheimer's disease (AD). The effects of this naturally occurring beta-amyloid on the cells of the central nervous system have not been completely understood. beta-Amyloid increases the vulnerability of cultured neurons to glutamate-induced excitotoxic damage. Because astrocytes play a key role in uptake of extracellular glutamate and glutamate uptake is ATP-dependent, we studied the effect of beta25-35 on glutamate and glucose uptake in cultured hippocampal astrocytes following 7 days of exposure to beta25-35. Astrocytic glutamate uptake was studied at 1, 5, 10, 15, 20, and 60 min following the addition of [3H]glutamate (5 nM) to the culture media, and astrocytic glucose uptake was assessed at 60 min after the addition of [14C]glucose (600 and 640 nM) to the media. Glutamate uptake by control astrocytes was time-dependent. Astrocytes exposed to beta25-35, however, showed significantly lower glutamate uptake at all sampling times. Similarly, [14C]glucose uptake by astrocytes was inhibited by beta25-35. When glucose uptake was blocked by phloretin (10 mM), astrocytic [3H]glutamate uptake was also blocked, suggesting that the inhibitory effect of beta-amyloid on glutamate uptake is caused by diminished glucose uptake. Thus, our present study suggests a possible link between two proposed mechanisms of pathogenesis of the Alzheimer's disease: glutamate neurotoxicity and global defect in cerebral energy metabolism.
journal_name
Brain Resjournal_title
Brain researchauthors
Parpura-Gill A,Beitz D,Uemura Edoi
10.1016/s0006-8993(97)00043-7subject
Has Abstractpub_date
1997-04-18 00:00:00pages
65-71issue
1-2eissn
0006-8993issn
1872-6240pii
S0006-8993(97)00043-7journal_volume
754pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Antibodies binding to the 150 kDa neurofilament protein NF150 have been purified from a serum raised by immunizing a rabbit with alpha-MSH. The NF150-binding antibodies were purified by affinity chromatography on a column of cytoskeletal proteins coupled to CNBr-activated Sepharose-4B. Immunocytochemical application o...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91365-5
更新日期：1987-02-24 00:00:00
abstract：:The disturbance of microcirculation following cerebral ischemia leads to an enlargement of cerebral infarct volume. Endogenous thrombin may play a role in this disturbance of microcirculation following cerebral ischemia. Therefore, the inhibition of thrombin may improve neurodegeneration and the accumulation of cerebr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02354-x
更新日期：2001-06-01 00:00:00
abstract：:In pentobarbitone-anesthetized cats breathing spontaneously, we studied whether excision of one petrosal ganglion would modify the reflex efficacy of the remaining carotid and aortic chemoafferences in ventilatory control. Resting ventilation was not affected shortly after the ganglionectomy, but decreased sensitiviti...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91495-3
更新日期：1990-07-23 00:00:00
abstract：BACKGROUND:Sex-specific trajectories in white matter development during adolescence may help explain cognitive and behavioral divergences between males and females. Knowledge of sex differences in typically developing adolescents can provide a basis for interpreting sexual dimorphisms in abilities and actions. METHOD:...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.12.051
更新日期：2011-02-23 00:00:00
abstract：:The effect of the sulfonamide derivative sulthiame (Ospolot) on voltage-operated sodium channels was investigated in acutely isolated neurons from the guinea pig hippocampus using the whole-cell patch-clamp technique. Sulthiame in a concentration of 10 microg/ml reduced the inactivating sodium currents without affecti...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02262-4
更新日期：2001-05-04 00:00:00
abstract：:Aspartame (L-aspartyl-L-phenylalanine methyl ester), an artificial low-calorie sweetener, was shown to dose-dependently inhibit L-[3H]glutamate binding to its N-methyl-D-aspartate-specific receptors. L-Aspartic acid, a major endogenous metabolite of aspartame, inhibited the binding more stronger than aspartame, while ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91729-z
更新日期：1990-06-18 00:00:00
abstract：:Genetic variability in the behavioral responses of experimental subjects to psychostimulants such as amphetamine and cocaine have been reported. However, genetic differences in the locomotor responses of rat strains to methylphenidate (MPD), a commonly used psychostimulant in the treatment of attention deficit/hyperac...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)04240-3
更新日期：2003-05-09 00:00:00
abstract：:Large neurons in the neostriatum of patients with Alzheimer's disease (AD) and progressive supranuclear palsy (PSP) were investigated topographically, histologically and ultrastructurally. The number of large neurons whose nuclear area is greater than 101 microns2 was uniformly decreased in the neostriatum in PSP, but...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90057-3
更新日期：1991-03-29 00:00:00
abstract：:The morphological features and distribution of luteinizing hormone-releasing hormone (LHRH)-immunoreactive cell bodies and fibers of the hypothalamic and the neighboring mesencephalic regions were studied in the normal newborn infant by immunohistochemistry. Within the hypothalamus, numerous LHRH-immunoreactive like (...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90929-6
更新日期：1990-05-21 00:00:00
abstract：:Horseradish peroxidase (HRP) in slow-release gel was unilaterally implanted in the transected dorsolateral funiculus (DLF) of either cervical, midthoracic, lumbar or sacral spinal cord levels of adult male rats. Cell mappings were made of all brain areas projecting through the DLF. Following cervical implants, dense l...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)91139-2
更新日期：1981-11-02 00:00:00
abstract：:Projections to the rostral ventrolateral medulla (RVLM) and nucleus raphe magnus (NRM) appear to originate from neurones with overlapping distributions in the periaqueductal grey (PAG) as demonstrated by the retrograde transport of red and green fluorescent latex microspheres. Furthermore, double-labelling studies dem...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00784-6
更新日期：1996-09-09 00:00:00
abstract：:The localization of calcitonin gene-related peptide (CGRP)-like immunoreactive (CGRPI) structures in the cochlea was examined in the rat using immunocytochemistry. Numerous CGRPI fibers entered the organ of Corti in the intraganglionic spiral bundle and formed a dense fiber patch at the base of the inner hair cells. M...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90992-8
更新日期：1985-12-09 00:00:00
abstract：:Here we describe a sustained outward potassium current (IK) in retinal horizontal cells (HCs). IK is unusually small over the range of membrane potentials normally experienced by these cells, which are chronically depolarized. We hypothesize that this unique IK will reduce the amount of neurotransmitter required to sh...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90204-o
更新日期：1990-09-24 00:00:00
abstract：:Experimental and genetic hypertension in male rate is accompanied by a lower specific [3H]naloxone binding in the dorsal horn of the spinal cord, and in the hippocampus as compared to controls. Rats which are genetically resistant to hypertensive stimuli have a higher specific [3H]naloxone binding in the nucleus tract...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)91265-8
更新日期：1981-05-25 00:00:00
abstract：:We used amygdala-kindled seizures in rats to study the function of striatal gamma-aminobutyrate (GABA) in the regulation of the seizure threshold of the limbic forebrain. Microinjections of the GABAA antagonist, bicuculline methiodide, 1 pmol, into the caudate-putamen protected rats against amygdala-kindled seizures. ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91089-4
更新日期：1987-05-19 00:00:00
abstract：:By using a proteomic approach, we found increased levels of carbonic anhydrase II (CA II) in the brain of Ts65Dn mice, a mouse model for Down syndrome (DS). Further immunoblot analyses showed that the levels of CA II are increased not only in the brain of adult Ts65Dn mice but also in the brain of infants and young ch...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.11.023
更新日期：2008-01-23 00:00:00
abstract：:A brief noxious heat stimulus applied to half of the receptive field of each cutaneous nociceptor resulted in most cases in the enhanced responsiveness of that half to subsequent heat stimuli (sensitization). No change in responsiveness to heat was observed in the other half of the receptive field, indicating that the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90364-x
更新日期：1982-01-14 00:00:00
abstract：:Microinjection of the indirect GABAA antagonist, picrotoxin, or the mu opioid agonist, Tyr-D-Ala-Gly-NMe-Phe-Gly-ol (DAGO), into the ventral pallidum and substantia innominata (VP/SI) increases locomotor activity in rats. The VP/SI has direct and indirect projections to the region of the ventral mesencephalon containi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91005-l
更新日期：1991-02-22 00:00:00
abstract：:Although platelet-derived growth factor (PDGF)-BB activates PDGF receptor-beta (PDGFR-beta) and, in turn, inhibits the glutamate N-methyl-D-aspartate (NMDA) receptor function, whether PDGF-BB modulates the CNS function mediated by another glutamate receptors, alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (A...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.05.037
更新日期：2007-07-23 00:00:00
abstract：:Cerebral autoregulation was tested here in 3- and 18-month-old spontaneously hypertensive rats (SHR) and Wistar Kyoto controls (WKY). Cerebral blood flow (CBF) was measured in each rat using radioactive micropheres under unanesthetized control conditions and after hypotension induced by ganglionic blockade. Under cont...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90454-6
更新日期：1981-06-09 00:00:00
abstract：:5-Hydroxytryptamine (5-HT, serotonin), synthesized in midbrain raphe nuclei and released in various hypothalamic sites, decreases food intake but the specific 5-HT receptor subtypes involved are controversial. Here, we have studied changes in the regional density of binding to 5-HT receptors and transporters and the l...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)02055-7
更新日期：1999-11-13 00:00:00
abstract：:The central type benzodiazepine receptor (BDZr), an allosteric modulatory site of the GABAA receptor-anion channel, has been shown in vitro to respond to drugs with positive efficacy (agonists), zero efficacy (competitive antagonists) and drugs with negative efficacy (inverse agonists). However, this general concept o...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90131-e
更新日期：1991-08-23 00:00:00
abstract：:In an autopsy study on 29 male Aborigines and 57 male Caucasians, macroscopic and microscopic parameters of postnatal cerebellar development were determined and described by mathematic growth functions. For the whole cerebellum and its cortical layers volume measurements revealed delayed maturation for Aborigines comp...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90782-8
更新日期：1989-10-16 00:00:00
abstract：:Astrocytes cultured from the brains of neonatal rat pups acquire at least two specializations of intramembrane particle distribution: 'assemblies' and gap junctions. The number and appearance of assemblies in the cultured astrocytes is not markedly influenced by the presence or absence of a collagen substrate, and the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90545-0
更新日期：1983-10-03 00:00:00
abstract：:The possible interference of nitric oxide (NO) in glucose metabolism was studied in activated astrocytes. Lipopolysaccharide (LPS) treatment triggered a NO-mediated increase in glucose consumption and lactate production, suggesting an enhanced rate of glycolysis. Active glycogen synthesis was also observed after LPS t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02877-9
更新日期：2002-07-26 00:00:00
abstract：:Differential circadian rhythms in 5-HT levels were found in the hypothalamus and pineal (but not in the cortex, hippocampus or midbrain) in ferrets kept in either long (14 h light/10 h dark) or short (8 h light/16 h dark) photoperiods. 5-HT decreased during the first 6 h of illumination in all areas examined from anim...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90986-7
更新日期：1979-11-02 00:00:00
abstract：:Intracellular recordings from locus coeruleus neurons grown in explant cultures reveal age-related differences in the responses to iontophoretically applied noradrenaline. Cells grown in culture for more than 26 days exhibit a simple hyperpolarizing response while cells cultured for less than 26 days often have biphas...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(86)80238-4
更新日期：1986-03-01 00:00:00
abstract：:In our previous study, we have found that leucine-rich repeats and immunoglobulin-like domains 1(LRIG1) can improve the chemosensitivity in U251 cells whereas the role of LRIG1 in multidrug resistance (MDR) remains unknown. Here, we reported that LRIG1 can reverse MDR by inhibiting epidermal growth factor (EGF) recept...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.03.023
更新日期：2015-06-22 00:00:00
abstract：:Norepinephrine (NE) and 3-methoxy-4-hydroxyphenylglycol (MOPEG) were determined in medio-basal hypothalamus of adult male rats using high-performance liquid chromatography to study nutritional modulation of noradrenergic turnover. Acute starvation, as well as 3 weeks of semistarvation with a low-protein high-carbohydr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90471-8
更新日期：1985-06-03 00:00:00
abstract：:In recent years microvillar cells (MVC) have been identified in the olfactory epithelium of numerous species, including rodents, canines, and primates. However, there is no consensus on the morphologic or histochemical features of this cell, nor is the function of these cells currently known. Previous studies have exa...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)01144-7
更新日期：1995-01-09 00:00:00
