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Attenuation of TGF-beta-induced apoptosis in primary cultures of hepatocytes by calpain inhibitors.

Abstract：

:Apoptosis induced in primary cultures of rat hepatocytes by transforming growth factor beta 1 (TGF-beta 1) was greatly attenuated by inhibitors (5 microM) of calpain I and calpain II, respectively. Both inhibitors prevented the TGF-beta-elicited increase of nucleosomal DNA fragments and the occurrence of DNA-breaks in the TUNEL reaction. The detrimental effect of TGF-beta on cell viability measured by the WST-1 test was strongly reduced by calpain inhibitors. Calpain II > I inhibitors suppressed spontaneous DNA cleavage in hepatocytes during culture and prevented the appearance of immunocytochemically visible TGF-beta (APAAP staining), which occurs in untreated parenchymal cell cultures. The data show that inactivation of calpains attenuates both the TGF-beta-elicited and the spontaneous apoptosis of cultured hepatocytes; the latter effect is likely due to the suppression of endogenous TGF-beta activation. It is suggested that calpains participate in these processes.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Gressner AM,Lahme B,Roth S

doi

10.1006/bbrc.1996.5777

subject

Has Abstract

pub_date

1997-02-13 00:00:00

pages

457-62

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)95777-0

journal_volume

231

pub_type

杂志文章

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