Functional analysis of α1,3/4-fucosyltransferase VI in human hepatocellular carcinoma cells.

abstract：:High sensitivity detection of DNA is essential for genomics. The intrinsic fluorescence from DNA is very weak and almost all methods for detecting DNA rely on the use of extrinsic fluorescent probes. We show that the intrinsic emission from DNA can be enhanced many-fold by spatial proximity to silver island films. Sil...

journal_title：Biochemical and biophysical research communications

authors： Lakowicz JR,Shen B,Gryczynski Z,D'Auria S,Gryczynski I

更新日期：2001-09-07 00:00:00

abstract：:Carbohydrate response element binding protein (ChREBP) is responsible for conversion of dietary carbohydrate to storage fat in liver by coordinating expression of the enzymes that channel glycolytic pyruvate into lipogenesis. The activation of ChREBP in response to high glucose is nuclear localization and transcriptio...

journal_title：Biochemical and biophysical research communications

authors： Fukasawa M,Ge Q,Wynn RM,Ishii S,Uyeda K

更新日期：2010-01-08 00:00:00

abstract：:Tuberous sclerosis complex 2 (TSC2) is a tumor-suppressor protein that is partially regulated by insulin, energy, oxygen, and growth factors. Mutations in the TSC2 gene and loss of TSC2 promote cell growth by the mammalian target of rapamycin complex 1 (mTORC1) activation. Furthermore, S-adenosylmethionine (SAM) senso...

journal_title：Biochemical and biophysical research communications

authors： Gen S,Matsumoto Y,Suzuki T,Inoue J,Yamamoto Y

更新日期：2021-01-19 00:00:00

abstract：:Kurtoxin, a 63-amino acid peptide stabilized by four disulfide bonds, is the first reported peptide inhibitor of T-type voltage-gated calcium channels. Although T-type calcium channels have been implicated in a number of disease states, including epilepsy, chronic pain, hypertension and cancer, the lack of selective i...

journal_title：Biochemical and biophysical research communications

authors： Lee CW,Eu YJ,Min HJ,Cho EM,Lee JH,Kim HH,Nah SY,Swartz KJ,Kim JI

更新日期：2011-12-16 00:00:00

abstract：:The efp, a member of the RING finger family, was previously identified as an estrogen responsive gene. Here, we characterized basal promoter of the human efp gene. Transcription initiation site was found at position -60 G relative to the site for translation initiation, and TATA motif was absent. Deletion and mutation...

journal_title：Biochemical and biophysical research communications

authors： Ikeda K,Inoue S,Orimo A,Sano M,Watanabe T,Tsutsumi K,Muramatsu M

更新日期：1997-07-30 00:00:00

abstract：:Large tumor suppressor kinase 1 (LATS1) is a serine/threonine kinase of the AGC kinase family in mammals and involved in various biological processes, it is a key regulator of cell cycle progression. However, the role of LATS1 in central nervous system trauma is still unknown. In present study, we performed an acute s...

journal_title：Biochemical and biophysical research communications

authors： Wang Y,Chen M

更新日期：2018-10-20 00:00:00

abstract：:Application of landscape theory and the dehydron hypothesis to a crystal structure of a G85R mutant superoxide dismutase (SOD1) tetrameric complex allows for the description of a prion-like hypothesis that serves to explain propagated SOD1 misfolding. We have developed two conformational-change scenarios, one local to...

journal_title：Biochemical and biophysical research communications

authors： Healy EF

更新日期：2016-09-30 00:00:00

abstract：:GGAs (Golgi-localizing, gamma-adaptin ear domain homology, ADP-ribosylation factor (ARF)-binding proteins), constitute a family of monomeric adaptor proteins and are associated with protein trafficking from the trans-Golgi network to endosomes. Here, we show that GGA3 is monoubiquitylated by a RING-H2 type-ubiquitin l...

journal_title：Biochemical and biophysical research communications

authors： Yogosawa S,Kawasaki M,Wakatsuki S,Kominami E,Shiba Y,Nakayama K,Kohsaka S,Akazawa C

更新日期：2006-11-10 00:00:00

abstract：:Coxsackievirus A24 (CVA24) is responsible for acute hemorrhagic conjunctivitis, a highly contagious eye disease for which no prevention or treatment is currently available. We thus assessed the antiviral potential of a small interfering RNA (siRNA) targeting CVA24. HeLa cells with or without four different siRNAs comp...

journal_title：Biochemical and biophysical research communications

authors： Jun EJ,Nam YR,Ahn J,Tchah H,Joo CH,Jee Y,Kim YK,Lee H

更新日期：2008-11-14 00:00:00

abstract：:Peripheral blood mononuclear cells (PBMCs) from normal adults secreted small amounts of human growth hormone (GH; 0.2-0.6 pg/10(5) cells/7 days culture) as measured by a highly sensitive enzyme immunoassay. Stimulation of PBMCs with phytohemagglutinin (PHA) consistently showed a 4-6 fold increase in GH secretion. Tran...

journal_title：Biochemical and biophysical research communications

authors： Hattori N,Shimatsu A,Sugita M,Kumagai S,Imura H

更新日期：1990-04-30 00:00:00

abstract：:Wild type p53 protein requires posttranslational modification within a carboxy-terminal negative regulatory domain to activate DNA binding and transcription. Binding of monoclonal antibody PAb421 to the carboxy-terminal domain reproduces this activation. In the absence of PAb421, we found that wild type p53 bound acti...

journal_title：Biochemical and biophysical research communications

authors： Kaku S,Albor A,Kulesz-Martin M

更新日期：2001-01-12 00:00:00

abstract：:Phospholipase C (PLC) has been known to be a key effector protein in signal transduction pathway for cell proliferation and differentiation. Here, we report the transcriptional regulation of PLC-gamma 1 during myogenic differentiation. Both PLC-gamma 1 mRNA and protein levels were increased during myogenesis. The acti...

journal_title：Biochemical and biophysical research communications

authors： Lee SJ,Lee YH,Kim YS,Ryu SH,Suh PG

更新日期：1995-01-05 00:00:00

abstract：:We examined how growth hormone-releasing factor (GRF) regulated pituitary GRF receptor gene expression in the conscious rat. GRF receptor mRNA levels were significantly increased by immunoneutralization of endogenous GRF with its specific antiserum. This effect was dose dependent and the maximum level was 3.8-fold hig...

journal_title：Biochemical and biophysical research communications

authors： Miki N,Ono M,Murata Y,Ohsaki E,Tamitsu K,Yamada M,Demura H

更新日期：1996-07-16 00:00:00

abstract：:The coding sequence of type II phospholipase A2 from human placenta was cloned in a bovine papilloma virus-derived eukaryotic expression vector under the control of the metallothionein promoter. Stably transfected C127 mouse fibroblast lines were obtained with this vector. These transfected cells overexpressed a funct...

journal_title：Biochemical and biophysical research communications

authors： Pernas P,Masliah J,Olivier JL,Salvat C,Rybkine T,Bereziat G

更新日期：1991-08-15 00:00:00

abstract：:Expression of ALP in F9 teratocarcinoma cells is induced by all-trans retinoic acid (ATRA) (Gianni' et al., Biochem. J. 274: 673-678, 1991). The specific ligand for retinoic acid related receptors (RXRs), 9-cis retinoic acid (9-cis RA), and three synthetic analogs binding to the alpha, beta and gamma forms of the reti...

journal_title：Biochemical and biophysical research communications

authors： Gianni M,Zanotta S,Terao M,Garattini S,Garattini E

更新日期：1993-10-15 00:00:00

abstract：:Lipopolysaccharides (LPS) of Porphyromonas gingivalis have been implicated in the initiation and development of periodontal diseases. In a previous study, we investigated the signal transduction pathway of P. gingivalis and demonstrated that LPS stimulates the production of interleukin (IL)-6 in human gingival fibrobl...

journal_title：Biochemical and biophysical research communications

authors： Wang PL,Shirasu S,Shinohar M,Azuma Y,Daito M,Yasuda H,Ohura K

更新日期：1999-09-24 00:00:00

abstract：:Necrotic cells are known to develop characteristic membrane blebs. We measured protein concentration within necrotic blebs and found that it can be reduced by as much as twenty-fold compared to the main cell body (CB). These results raise two questions: 1. Why do proteins vacate the bleb? 2. How can osmotic equilibriu...

journal_title：Biochemical and biophysical research communications

authors： Rana PS,Mudrak NJ,Lopez R,Lynn M,Kershner L,Model MA

更新日期：2017-10-21 00:00:00

abstract：:Glutathione S-transferase Y-2 mRNA synthesis was induced in yeast Issatchenkia orientalis approximately 37-fold by cultivation with o-dinitrobenzene. A cDNA library complementary to poly (A)+RNA of I. orientalis grown with o-dinitrobenzene was screened by colony hybridization. Twenty positive clones were obtained from...

journal_title：Biochemical and biophysical research communications

authors： Tamaki H,Kumagai H,Tochikura T

更新日期：1990-10-30 00:00:00

abstract：:Sphingolipids are essential components of eukaryotic cell membranes. We recently showed that the function of the serotonin(1A) receptor is impaired upon metabolic depletion of sphingolipids using fumonisin B(1) (FB(1)), a specific inhibitor of ceramide synthase. Serotonin(1A) receptors belong to the family of G-protei...

journal_title：Biochemical and biophysical research communications

authors： Ganguly S,Paila YD,Chattopadhyay A

更新日期：2011-07-22 00:00:00

abstract：:Expression of facilitative glucose transporters (Glut 1, 2 and 3) was examined by Western blot analyses 10, 20 and 30 days following surgically induced unilateral abdominal cryptorchidism. The cryptorchid testes exhibited marked degenerative changes in the seminiferous tubules and spermatogonia, impaired and incomplet...

journal_title：Biochemical and biophysical research communications

authors： Farooqui SM,Al-Bagdadi F,O'Donnell JM,Stout R

更新日期：1997-07-18 00:00:00

abstract：:Spiroplasma eriocheiris, the cause of crab trembling disease, is a wall-less bacterium, related to Mycoplasmas, measuring 2.0-10.0 μm long. It features a helical cell shape and a unique swimming mechanism that does not use flagella; instead, it moves by switching the cell helicity at a kink traveling from the front to...

journal_title：Biochemical and biophysical research communications

authors： Terahara N,Tulum I,Miyata M

更新日期：2017-06-03 00:00:00

abstract：:The reactivity of human thioredoxin (HTR) was tested in several reactions. HTR was as efficient as E. coli or plant and algal thioredoxins when assayed with E. coli ribonucleotide reductase or for the reduction of insulin. On the other hand, HTR was poorly reduced by NADPH and the E. coli flavoenzyme NADPH thioredoxin...

journal_title：Biochemical and biophysical research communications

authors： Jacquot JP,de Lamotte F,Fontecave M,Schürmann P,Decottignies P,Miginiac-Maslow M,Wollman E

更新日期：1990-12-31 00:00:00

abstract：:By radiolabeling using NaIO4/[3H]KBH4, a new sialoglycoprotein with Mr of 19,000 was found in the secretion of rat seminal vesicle. It was shown to interact non-covalently with semen coagulum. It existed in three acidic forms with pI values of 4.1, 3.7 and 2.9 and possessed high contents of sialic acids and acidic ami...

journal_title：Biochemical and biophysical research communications

authors： Limpaseni T,Chulavatnatol M

更新日期：1986-04-29 00:00:00

abstract：:Transgenic mice expressing the rhodopsin mutant Pro347Ser (Serine 6) display retinal degeneration through apoptosis that is characteristic of the disease retinitis pigmentosa. By 5 weeks after birth, these mice have lost approximately 35% of their photoreceptor cells. Retinas from these mice showed higher levels of cA...

journal_title：Biochemical and biophysical research communications

authors： Weiss ER,Hao Y,Dickerson CD,Osawa S,Shi W,Zhang L,Wong F

更新日期：1995-11-22 00:00:00

abstract：:The biological function of hnRNP family proteins is widely diverse and involved in pre-mRNA processing, transcriptional regulation, recombination, and telomere maintenance. In the course of our study on the elucidation of biological functions of minisatellite DNA, we isolated several nuclear proteins that bind to the ...

journal_title：Biochemical and biophysical research communications

authors： Tanaka E,Fukuda H,Nakashima K,Tsuchiya N,Seimiya H,Nakagama H

更新日期：2007-06-29 00:00:00

abstract：:In this paper, based on the approach by combining the "functional domain composition" [K.C. Chou, Y. D. Cai, J. Biol. Chem. 277 (2002) 45765] and the pseudo-amino acid composition [K.C. Chou, Proteins Struct. Funct. Genet. 43 (2001) 246; Correction Proteins Struct. Funct. Genet. 2044 (2001) 2060], the Nearest Neighbou...

journal_title：Biochemical and biophysical research communications

authors： Cai YD,Chou KC

更新日期：2003-05-30 00:00:00

abstract：:We have demonstrated that oral charcoal therapy is as effective as therapeutic phlebotomy in reducing porphyrinemia in porphyria cutanea tarda. The effects of immediate and sustained reduction of porphyrinemia on the catalytic properties of partially purified (approximately 200-fold) preparations of red cell uroporphy...

journal_title：Biochemical and biophysical research communications

authors： Mukerji SK,Pimstone NR

更新日期：1988-07-15 00:00:00

abstract：:The recent discovery that mutation Asn21 --> Ile in the human cationic trypsinogen (Tg) is associated with hereditary pancreatitis has brought into focus the functional role of amino acid 21 in mammalian Tgs. In the present paper, the effect of mutations Thr21 --> Asn and Thr21 --> Ile on the Ca(2+) dependence of zymo...

journal_title：Biochemical and biophysical research communications

authors： Sahin-Tóth M,Tóth M

更新日期：2000-08-28 00:00:00

abstract：:Release by phosphatidylinositol-specific phospholipase C is frequently provided as evidence for membrane anchorage of a protein through phosphatidylinositol. Demonstration that the enzyme removes a lipophilic moiety from the protein is stronger evidence, and is presented here for members of the Ly-6 family of lymphocy...

journal_title：Biochemical and biophysical research communications

authors： Hammelburger JW,Palfree RG,Sirlin S,Hämmerling U

更新日期：1987-11-13 00:00:00

abstract：:ATP is the universal energy currency but mysteriously its cellular concentration is much higher than that needed for providing energy. Recently ATP was decoded to act as a hydrotrope to dissolve liquid-liquid phase separation (LLPS) of FUS whose aggregation leads to ALS/FTD. By DIC microscopy and NMR, here we characte...

journal_title：Biochemical and biophysical research communications

authors： Kang J,Lim L,Song J

更新日期：2018-10-02 00:00:00