The interaction domains of transient receptor potential canonical (TRPC)1/4 and TRPC1/5 heteromultimeric channels.

abstract：:Calcitonin gene-related peptide (CGRP) in porcine ventricular muscles. positive inotropic effects in the isolated, electrically driven false tendon of the porcine heart. Specific CGRP-binding sites were present in solubilized membrane fractions; the dissociation constant (Kd) and the maximum binding (Bmax) were 50.4 p...

journal_title：Biochemical and biophysical research communications

authors： Miyauchi T,Sano Y,Hiroshima O,Yuzuriha T,Sugishita Y,Ishikawa T,Saito A,Goto K

更新日期：1988-08-30 00:00:00

abstract：:Two forms of human placental alkaline phosphatase have been purified to homogeneity utilizing high performance liquid chromatography. Both have the same amino acid composition but they differ in their carbohydrate substituents. Sequence data indicate that the two forms are identical for the first forty two residues fr...

journal_title：Biochemical and biophysical research communications

authors： Ezra E,Blacher R,Udenfriend S

更新日期：1983-11-15 00:00:00

abstract：:Mutations in mitochondrial DNA is one of the important causes of hearing loss. Here, we performed a mutational screening of tRNA(Ser(UCN)) gene in 1542 Chinese subjects with hearing loss. One subject and five subjects carried tRNA(Ser(UCN)) A7445C and G7444A mutations, respectively, while two subjects harbored both G7...

journal_title：Biochemical and biophysical research communications

authors： Jin L,Yang A,Zhu Y,Zhao J,Wang X,Yang L,Sun D,Tao Z,Tsushima A,Wu G,Xu L,Chen C,Yi B,Cai J,Tang X,Wang J,Li D,Yuan Q,Liao Z,Chen J,Li Z,Lu J,Guan MX

更新日期：2007-09-14 00:00:00

abstract：:Superoxide production by neutrophils induced by 1-oleoyl-2-acetyl-sn-glycerol at concentrations below 100 microM was enhanced by extracellular calcium ions, while that of phorbol myristate acetate was unaffected. Verapamil, a calcium-channel blocker, more effectively inhibited the superoxide production induced by 1-ol...

journal_title：Biochemical and biophysical research communications

authors： Ozaki Y,Kume S,Ohashi T,Niwa Y

更新日期：1986-01-29 00:00:00

abstract：:A simple procedure based upon the principle discovered by Dürr et al. (Arch. Microbiol. (1975) 105, 319-327) was used to measure the intracellular distribution of enzymes in S. cerevisiae grown under both glucose repression and derepression. No substantial hexokinase activity was found to be associated with cellular o...

journal_title：Biochemical and biophysical research communications

authors： Kovác L,Nelson BD,Ernster L

更新日期：1986-01-14 00:00:00

abstract：:The hydrolysis of p-nitrophenylphosphorylcholine, p-nitrophenylphosphate, and phosphorylcholine was quantitated in homogenates prepared from the occipital and temporal regions of control and Alzheimer's Disease patient brain. There were no significant differences detected in activities of enzymes cleaving the first 2 ...

journal_title：Biochemical and biophysical research communications

authors： Kanfer JN,McCartney DG

更新日期：1986-08-29 00:00:00

abstract：:A voltage-dependent K+ current has been revealed in whole-cell recordings carried out on immortalized cells obtained from the human tracheal epithelium. At positive membrane potentials the current shows a time dependent inactivation which is accelerated by increasing the depolarizing step. Forskolin, a direct activato...

journal_title：Biochemical and biophysical research communications

authors： Galietta LJ,Rasola A,Barone V,Gruenert DC,Romeo G

更新日期：1991-09-30 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is known to bind to the F-actin cytoskeleton in intact cells, and this interaction has been suggested to sequester the EGFR signal transduction system to specific loci within the cell. In this study, the interaction of the EGFR with actin is examined in a reconstituted cell ...

journal_title：Biochemical and biophysical research communications

authors： Tang J,Gross DJ

更新日期：2003-12-26 00:00:00

abstract：:Butein, a plant polyphenol, was shown to be a specific protein tyrosine kinase inhibitor. This compound inhibited not only the epidermal growth factor (EGF)-stimulated auto-phosphotyrosine level of EGF receptor in HepG2 cells but also tyrosine-specific protein kinase activities of EGF receptor (IC50 = 65 microM) and p...

journal_title：Biochemical and biophysical research communications

authors： Yang EB,Zhang K,Cheng LY,Mack P

更新日期：1998-04-17 00:00:00

abstract：:A synthetic inhibitor of calpain protects rat erythrocyte membrane-associated cytoskeletal proteins from proteolytic degradation (IC50 = 1 microM) which occurs when the cells are rendered permeable to Ca++. Leupeptin, a naturally occurring inhibitor of the enzyme, does not afford any protection at concentrations up to...

journal_title：Biochemical and biophysical research communications

authors： Mehdi S,Angelastro MR,Wiseman JS,Bey P

更新日期：1988-12-30 00:00:00

abstract：:Plasma membrane receptors can undergo translocation in the plane of plasma membrane after binding of polyvalent ligands. Ligand/receptor clusters, named patches, can collect into a polar cap, presumably due to their association with the submembrane actin-based cytoskeleton. We found that the assembly of Fcgamma recept...

journal_title：Biochemical and biophysical research communications

authors： Kwiatkowska K,Sobota A

更新日期：1999-06-07 00:00:00

abstract：:Radiation induced fibrosis occurs following a therapeutic or accidental radiation exposure in normal tissues. Tissue fibrosis is the excessive accumulation of collagen and other extracellular matrix components. This study investigated how ionizing radiation affects the expression level and signal pathway of type I col...

journal_title：Biochemical and biophysical research communications

authors： Yano H,Hamanaka R,Nakamura M,Sumiyoshi H,Matsuo N,Yoshioka H

更新日期：2012-02-17 00:00:00

abstract：:Mutations in the myocilin (MYOC), also known as Trabecular meshwork-Inducible Glucocorticoid Response (TIGR) gene can lead to juvenile open-angle glaucoma in human and may be responsible for at least 3% of primary open-angle glaucoma. To develop a mouse model of primary open angle glaucoma, and to get deeper insight i...

journal_title：Biochemical and biophysical research communications

authors： Tomarev SI,Tamm ER,Chang B

更新日期：1998-04-28 00:00:00

abstract：:Dexamethasone (Dex) causes osteoblast cell injuries. In the present research, we tested the potential effect of SC79, a novel and specific Akt activator, against Dex in osteoblasts. In primary murine osteoblasts and osteoblastic MC3T3-E1 cells, pretreatment with SC79 significantly attenuated Dex-induced cell death. Fu...

journal_title：Biochemical and biophysical research communications

authors： Li ST,Chen NN,Qiao YB,Zhu WL,Ruan JW,Zhou XZ

更新日期：2016-10-07 00:00:00

abstract：:We have identified a genomic DNA fragment, using the PCR method with degenerate oligonucleotide primers which contain the conserved sequence of the RING finger domain. Using the DNA fragment as a probe, a novel cDNA was cloned from human and mouse testis. The cDNA had a domain structure of the typical RING-B box-coile...

journal_title：Biochemical and biophysical research communications

authors： Orimo A,Yamagishi T,Tominaga N,Yamauchi Y,Hishinuma T,Okada K,Suzuki M,Sato M,Nogi Y,Suzuki H,Inoue S,Yoshimura K,Shimizu Y,Muramatsu M

更新日期：2000-09-16 00:00:00

abstract：:DNA topoisomerase II (Topo II) is an essential enzyme that catalyzes topological changes of DNA and consists of a major member of mitotic chromosomes. To investigate the dynamic localization of Topo II in nuclei, we engineered the strain of Aspergillus nidulans expressing Topo II fused with green fluorescent protein (...

journal_title：Biochemical and biophysical research communications

authors： Kawagishi M,Akashi T,Kikuchi A

更新日期：2005-08-26 00:00:00

abstract：:RNAs isolated from normal and spontaneously hypertensive (SHR) rat heart tissues were examined by direct hybridization assay using cloned DNA probes containing chicken cardiac myosin light chain and heavy chain specific cDNA sequences. In 7 weeks old SHR heart, the level of mRNA hybridizable to these probes is the sam...

journal_title：Biochemical and biophysical research communications

authors： Delaney P,Vitek S,Cribbs L,Siddiqui MA

更新日期：1985-10-30 00:00:00

abstract：:Cot/Tpl2, a member of MAP kinase kinase kinase (MAPKKK), is indispensable for the ERK activation, as well as the production of TNF-α, IL-1β, IL-23, and PGE(2) in lipopolysaccharide (LPS)-stimulated macrophages. However, the expression and the functional roles of Cot/Tpl2 in mast cells have not been elucidated. The adm...

journal_title：Biochemical and biophysical research communications

authors： Chiba N,Kakimoto K,Masuda A,Matsuguchi T

更新日期：2010-11-05 00:00:00

abstract：:The cDNA encoding Mn peroxidase isozyme H4 from Phanerochaete chrysosporium was recombined into a baculovirus and heterologously expressed in Sf9 cells. The recombinant Mn peroxidase has the same molecular weight as the native enzyme as determined by SDS-PAGE and cross-reacts with a Mn peroxidase-specific antibody. Th...

journal_title：Biochemical and biophysical research communications

authors： Pease EA,Aust SD,Tien M

更新日期：1991-09-16 00:00:00

abstract：:Hepatocyte expression of inducible nitric oxide synthase (iNOS) is initiated by the presence of pro-inflammatory cytokines, such as interleukin-1beta (IL-1). In the presence of oxidative stress, IL-1beta mediated hepatocyte iNOS expression and NO synthesis are significantly increased. To determine the underlying molec...

journal_title：Biochemical and biophysical research communications

authors： Kuo PC,Abe KY,Schroeder RA

更新日期：1997-05-19 00:00:00

abstract：:Co-transcriptional RNA proofreading by RNA polymerase (RNAP) is essential for accurate mRNA synthesis and reactivation of stalled transcription complexes, which can otherwise compromise genome integrity. RNAP from the stress-resistant bacterium Deinococcus radiodurans exhibits high levels of RNA cleavage in comparison...

journal_title：Biochemical and biophysical research communications

authors： Esyunina D,Kulbachinskiy A

更新日期：2019-01-29 00:00:00

abstract：:Familial exudative vitreoretinopathy (FEVR) is a congenital hereditary, bilateral eye disorder which affects both retina and the vitreous body. As a first step toward the identification of the gene responsible for the X-linked disorder, we report here the results of DNA analyses from the patients and their parents of ...

journal_title：Biochemical and biophysical research communications

authors： Shastry BS,Trese MT

更新日期：1993-06-15 00:00:00

abstract：:Fanconi anemia (FA) is a genetic disorder characterized by congenital malfunction, bone marrow failure and hypersensitivity to DNA damage. FANCD2 protein play the central role in FA pathway. To study the in vivo role of FANCD2, we generated and characterized a new Fancd2 knockout mouse strain with 7bp deletion in Fanc...

journal_title：Biochemical and biophysical research communications

authors： Yang Q,Xie H,Zhong Y,Li D,Ke X,Ying H,Yu B,Zhang T

更新日期：2019-06-30 00:00:00

abstract：:The most recently described region of alternate splicing of fibronectin mRNA results in expression of an isoform which includes the type III repeat termed ED B (EIII B). To date this isoform has been detected in transformed cells in culture, in the synovial membrane and ovary but in no other adult tissue, and in embry...

journal_title：Biochemical and biophysical research communications

authors： Burton-Wurster N,Lust G,Wert R

更新日期：1989-12-15 00:00:00

abstract：:Long-term glucocorticoid (GC) usage may cause non-traumatic femoral head osteonecrosis. Dexamethasone (Dex) is shown to exert potent cytotoxic effect to osteoblasts. Here, we investigated the potential activity of α-melanocyte stimulating hormone (α-MSH) against the process. Our data revealed that pretreatment of α-MS...

journal_title：Biochemical and biophysical research communications

authors： Guo S,Xie Y,Fan JB,Ji F,Wang S,Fei H

更新日期：2016-01-08 00:00:00

abstract：:Hepatitis C virus (HCV) is dependent on NS2-3 autocleavage and NS5A phosphorylation for its life cycle. We demonstrate that NS5A, when released from the NS2-5 polyprotein of the BK virus strain, is phosphorylated as two distinct forms, pp56 and pp58. Deletion analysis indicates that the appearance of pp58 requires NS2...

journal_title：Biochemical and biophysical research communications

authors： Liu Q,Bhat RA,Prince AM,Zhang P

更新日期：1999-01-27 00:00:00

abstract：:Posttranslational histone modifications play an important role in modulating gene expression and chromatin structure. Here we report the identification of histone H3K79 dimethylation in the simple eukaryote Dictyostelium discoideum. We have deleted the D. discoideum Dot1/KMT4 homologue and demonstrate that it is the s...

journal_title：Biochemical and biophysical research communications

authors： Müller-Taubenberger A,Bönisch C,Fürbringer M,Wittek F,Hake SB

更新日期：2011-01-28 00:00:00

abstract：:Antigen-specific regulatory CD4(+) T cells have been described but there are few reports on regulatory CD8(+) T cells. We generated islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP)-specific regulatory CD8(+) T cells from 8.3-NOD transgenic mice. CD8(+) T cells from 8.3-NOD splenocytes were...

journal_title：Biochemical and biophysical research communications

authors： Kishi M,Yasuda H,Abe Y,Sasaki H,Shimizu M,Arai T,Okumachi Y,Moriyama H,Hara K,Yokono K,Nagata M

更新日期：2010-03-26 00:00:00

abstract：:SAMHD1 (Sterile alpha motif and histidine-aspartic acid (HD) domain containing protein 1) is a deoxyribonucleoside triphosphate (dNTP) triphosphohydrolase (dNTPase) that restricts viral replication in infected cells. This protein is also involved in DNA repair by assisting in DNA end resection by homologous recombinat...

journal_title：Biochemical and biophysical research communications

authors： Medeiros AC,Soares CS,Coelho PO,Vieira NA,Baqui MMA,Teixeira FR,Gomes MD

更新日期：2018-05-23 00:00:00

abstract：:The 3.6 kb HindIII DNA fragment of B. sphaericus 1593M chromosomal DNA bears two genes encoding two polypeptides of 41.9 kDa (protein "42") and 51.4 kDa (protein "51"). DNA fragments carrying only one of these two genes when expressed in E. coli yield products that are inactive towards Culex larvae. The larvicidal act...

journal_title：Biochemical and biophysical research communications

authors： de la Torre F,Bennardo T,Sebo P,Szulmajster J

更新日期：1989-11-15 00:00:00