A method for determining the intracellular distribution of enzymes in yeast provides no evidence for the association of hexokinase with mitochondria.

abstract：:Parkinson's disease (PD) is neurodegenerative disease, featured by a loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc), characteristic motor symptoms and cognitive impairment. Development of effective therapeutic drugs for PD is necessary. In this study, we investigated the potential of Brucein...

journal_title：Biochemical and biophysical research communications

authors： Yang Y,Kong F,Ding Q,Cai Y,Hao Y,Tang B

更新日期：2020-06-11 00:00:00

abstract：:The cDNA encoding a swelling-induced chloride conductance regulatory protein, plcln, was cloned from rabbit ciliary epithelium by using a polymerase chain reaction (PCR)-based approach. The open reading frame encoding 236 amino acids possesses high amino acid identity (93/%) with the previously cloned plcln from human...

journal_title：Biochemical and biophysical research communications

authors： Wan XL,Chen S,Sears M

更新日期：1997-10-29 00:00:00

abstract：:The Monte Carlo method has been used to simulate the equilibrium properties of a planar array of 94 saturated lipid chains and one monomer of Gramicidin A. Chains are free to move laterally in the layer plane and to change conformation via gauche rotations and long axis rotations in a continuum. All non-hydrogen atoms...

journal_title：Biochemical and biophysical research communications

authors： Xing J,Scott HL

更新日期：1989-11-30 00:00:00

abstract：:We identified 13 transcript isoforms of a trinucleotide-repeat-containing gene, cag-8, that is expressed almost exclusively in the mouse brain. The polypeptide deduced from the cDNA consists of 137 AAs, of which 74 are glutamines. The 130-kb gene is composed of 16 exons, from which at least 13 isoforms with variable U...

journal_title：Biochemical and biophysical research communications

authors： Ji JW,Yang HL,Kim SJ

更新日期：2006-08-11 00:00:00

abstract：:Myeloperoxidase (MPO) is a glycosylated heme-containing enzyme present in the azurophilic granules of normal human polymorphonuclear neutrophils. This enzyme plays a major role in the microbicidal activity of the host defense system by catalyzing the formation of the potent oxidant, hypochlorous acid. Although the ami...

journal_title：Biochemical and biophysical research communications

authors： Taylor KL,Uhlinger DJ,Kinkade JM Jr

更新日期：1992-09-30 00:00:00

abstract：:Nitric oxide (NO) produced by macrophages plays an important role in host defense and inflammation. We found that two agrochemicals, alachlor and carbaryl, inhibit lipopolysaccharide (LPS)-induced NO production by macrophages. In the present study, we investigated this inhibitory mechanism in RAW 264 cells. Both chemi...

journal_title：Biochemical and biophysical research communications

authors： Shimomura-Shimizu M,Sugiyama K,Muroi M,Tanamoto K

更新日期：2005-07-08 00:00:00

abstract：:A melanin (melanosome) concentrating hormone, MCH, was synthesized and the methodology for its synthesis is detailed. This heptadecapeptide, H-Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val-OH , stimulated melanosome concentration (centripetal aggregation) within melanophores of all species of tel...

journal_title：Biochemical and biophysical research communications

authors： Wilkes BC,Hruby VJ,Sherbrooke WC,Castrucci AM,Hadley ME

更新日期：1984-07-31 00:00:00

abstract：:This study evaluated the effects of in vivo administration of human chorionic gonadotropin (hCG) on c-myc oncogene expression in Leydig cells. Sprague-Dawley rats (46-50 days old) were treated with hCG (10 units, i.p.), and purified Leydig cells were isolated 1-24 h later. HCG caused a transient elevation of c-myc mRN...

journal_title：Biochemical and biophysical research communications

authors： Lin T,Blaisdell J,Barbour KW,Thompson EA

更新日期：1988-11-30 00:00:00

abstract：:S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTases) methylate diverse biological molecules using a SAM cofactor. The ytqB gene of Bacillus subtilis encodes a putative MTase and its biological function has never been characterized. To reveal the structural features and the cofactor binding mode of YtqB,...

journal_title：Biochemical and biophysical research communications

authors： Park SC,Song WS,Yoon SI

更新日期：2014-04-18 00:00:00

abstract：:We have previously shown that the transcription factor C/EBP delta is involved in the intestinal inflammatory response. C/EBP delta regulates several inflammatory response genes, such as haptoglobin, in the rat intestinal epithelial cell line IEC-6 in response to IL-1. However, the different C/EBP delta domains involv...

journal_title：Biochemical and biophysical research communications

authors： Svotelis A,Doyon G,Bernatchez G,Désilets A,Rivard N,Asselin C

更新日期：2005-03-11 00:00:00

abstract：:We measured the amounts of total A beta, A beta 1-40 and A beta 1-42/43 in brain tissues using a newly developed ELISA assay and found that the amounts of insoluble A beta 1-42/43 and insoluble A beta 1-40 were linearly related to the amount of A beta deposits or total insoluble A beta at their lower and higher concen...

journal_title：Biochemical and biophysical research communications

authors： Tamaoka A,Kondo T,Odaka A,Sahara N,Sawamura N,Ozawa K,Suzuki N,Shoji S,Mori H

更新日期：1994-11-30 00:00:00

abstract：:Exosomes are 50-100-nm-diameter membrane vesicles released from various types of cells. Exosomes retain proteins, mRNAs and miRNAs, which can be transported to surrounding cells. CD109 is a glycosylphosphatidylinositol-anchored glycoprotein, and is released from the cell surface to the culture medium in vitro. Recentl...

journal_title：Biochemical and biophysical research communications

authors： Sakakura H,Mii S,Hagiwara S,Kato T,Yamamoto N,Hibi H,Takahashi M,Murakumo Y

更新日期：2016-01-22 00:00:00

abstract：:Mast cells play an important role in allergic inflammation by releasing inducible proinflammatory cytokines. While many inducible genes have been identified, we hypothesized that a significant number remain to be identified. We thus constructed an activation-specific mast cell subtraction library to establish a profil...

journal_title：Biochemical and biophysical research communications

authors： Cho JJ,Vliagoftis H,Rumsaeng V,Metcalfe DD,Oh CK

更新日期：1998-01-06 00:00:00

abstract：:To elucidate whether p21waf-1/cip-1/sdi-1 expression is associated with loss of growth potential of hepatocytes of old rats, we determined p21waf-1/cip-1/sdi-1 expression of hepatocytes from old (30 months) rats during the cell cycle in primary culture. A high level of expression of p21waf-1/cip-1/sdi-1 was detected a...

journal_title：Biochemical and biophysical research communications

authors： Sawada N,Kojima T,Obata H,Saitoh M,Isomura H,Kokai Y,Satoh M,Mori M

更新日期：1996-11-21 00:00:00

abstract：:Interleukin (IL)-19 belongs to the IL-10 family which includes IL-19, IL-20, IL-22, AK155, and MDA-7. IL-10 is a potent immunomodulatory cytokine with implications for pathogenesis in various autoimmune diseases. Polymorphism of the IL-10 promoter region correlates with disease outcome. To understand the gene regulati...

journal_title：Biochemical and biophysical research communications

authors： Chen PJ,Wei CC,Wang C,Chen FW,Hsu YH,Chang MS

更新日期：2006-06-09 00:00:00

abstract：:A novel N-terminally substituted Pro(3) analogue of glucose-dependent insulinotropic polypeptide (GIP) was synthesized and tested for plasma stability and biological activity both in vitro and in vivo. Native GIP was rapidly degraded by human plasma with only 39 +/- 6% remaining intact after 8 h, whereas (Pro(3))GIP w...

journal_title：Biochemical and biophysical research communications

authors： Gault VA,O'Harte FP,Harriott P,Flatt PR

更新日期：2002-02-08 00:00:00

abstract：:AtNPF7.3/AtNRT1.5, which is a nitrate transporter that drives root-to-shoot transport of NO3-, is also involved in modulating the response to K+ deprivation in Arabidopsis by affecting root development and K+ transport. However, whether NPF7.3/NRT1.5 functions in regulating plant responses to deficiencies of other nut...

journal_title：Biochemical and biophysical research communications

authors： Cui YN,Li XT,Yuan JZ,Wang FZ,Wang SM,Ma Q

更新日期：2019-01-01 00:00:00

abstract：:Screening for inhibitors of DNA-dependent protein kinase (DNA-PK) revealed 3-cyano-5-(4-pyridyl)-6-hydrazonomethyl-2-pyridone, designated OK-1035, to be a potent and selective inhibitor. When a synthetic peptide was used as a substrate, OK-1035 caused 50% inhibition of DNA-PK activity at 8 microM, a concentration more...

journal_title：Biochemical and biophysical research communications

authors： Take Y,Kumano M,Hamano Y,Fukatsu H,Teraoka H,Nishimura S,Okuyama A

更新日期：1995-10-04 00:00:00

abstract：:The effect of platelet release products on cytosolic calcium [( Ca++]i) was examined by monitoring the fluorescence of chick embryonic heart cells loaded with the fluorescent calcium indicator indo-1 AM. Cell free filtrate of platelet release products was obtained from rabbit platelets activated with thrombin or colla...

journal_title：Biochemical and biophysical research communications

authors： Chien WW,Mohabir R,Newman D,Leung LL,Clusin WT

更新日期：1990-08-16 00:00:00

abstract：:Sortase enzymes belong to a family of transpeptidases found in Gram-positive bacteria. Sortase is responsible for the reaction that anchors surface protein virulence factors to the peptidoglycan cell wall of the bacteria. The compound (Z)-3-(2,5-dimethoxyphenyl)-2-(4-methoxyphenyl) acrylonitrile (DMMA) has previously ...

journal_title：Biochemical and biophysical research communications

authors： Oh KB,Nam KW,Ahn H,Shin J,Kim S,Mar W

更新日期：2010-05-28 00:00:00

abstract：:The mRNA for the novel aldehyde dehydrogenase 5 (ALDH5) gene was detected in HuH7 hepatoma cells. The cells also expressed cytosolic aldehyde dehydrogenase (ALDH1) mRNA, but no mitochondrial aldehyde dehydrogenase (ALDH2) mRNA. Extracts of the hepatoma cells contained an enzymatic activity with an isoelectric point si...

journal_title：Biochemical and biophysical research communications

authors： Stewart MJ,Malek K,Xiao Q,Dipple KM,Crabb DW

更新日期：1995-06-06 00:00:00

abstract：:The Mr approximately equal to 90,000 heat shock protein, hsp90, readily interacts with the glucocorticoid receptor to form the 9 S, non-DNA-binding receptor complex. This receptor is stabilized in cytosolic preparations by sodium molybdate. In analogy, sodium molybdate stabilizes a 9 S form of the dioxin receptor. Pol...

journal_title：Biochemical and biophysical research communications

authors： Denis M,Cuthill S,Wikström AC,Poellinger L,Gustafsson JA

更新日期：1988-09-15 00:00:00

abstract：:Carnitine deficiency associated with impairment of muscle function has been described in infants and young adults as a result of inherited or acquired disease but there are no reports on carnitine levels in healthy aging people. In this paper we show a marked reduction of carnitine and its derivatives in muscle, and o...

journal_title：Biochemical and biophysical research communications

authors： Costell M,O'Connor JE,Grisolía S

更新日期：1989-06-30 00:00:00

abstract：:Tri-iodothyronine stimulates neurotensin secretion from rat hypothalami in vitro. Propylthiouracil treatment of rats is associated with a decrease in anterior pituitary neurotensin content, an increase in hypothalamic content and an impaired release of peptide from hypothalami in vitro. In addition neurotensin has a d...

journal_title：Biochemical and biophysical research communications

authors： Sheppard MC,Askew RD,Shennan KI,Franks S,Ramsden DB

更新日期：1983-05-31 00:00:00

abstract：:Alpha-crystallin high-molecular-weight (HMW) aggregates can be formed in vitro by many mechanisms, but the mechanism of in vivo aggregation has not been clearly established. HMW and LMW (low-molecular-weight) alpha-crystallins were isolated from human lenses 50-60 years of age and some spectroscopic measurements were ...

journal_title：Biochemical and biophysical research communications

authors： Liang JJ,Akhtar NJ

更新日期：2000-08-28 00:00:00

abstract：:This study investigates whether angiotensin converting enzyme (ACE) inhibitors can block high glucose-induced increase of angiotensin II (Ang II) levels in cultured rat mesangial cells. Incubation of cells with high glucose for 5 days increased Ang II in the cell media (extracellular) and cell lysates (intracellular) ...

journal_title：Biochemical and biophysical research communications

authors： Singh R,Leehey DJ

更新日期：2007-06-15 00:00:00

abstract：:Critical analysis of the data published so far concerning the TSG101 gene revealed some inconsistencies leading us to its re-evaluation in 80 breast, brain, colon, and neuroectodermal tumors and 37 normal tissue specimens. In this study, the occurrence of TSG101 cDNA aberrant transcripts was verified, but in addition ...

journal_title：Biochemical and biophysical research communications

authors： Hampl M,Hampl J,Plaschke J,Fitze G,Schackert G,Saeger HD,Schackert HK

更新日期：1998-07-30 00:00:00

abstract：:Three small serum proteins (SSP-1, -2, and -3), with molecular masses of 6.5-10kDa, were isolated from Habu (Trimeresurus flavoviridis) serum, and the amino acid sequences were determined by protein and cDNA analysis. Despite only limited sequence identity to any mammalian prostatic secretory protein of 94 amino acids...

journal_title：Biochemical and biophysical research communications

authors： Aoki N,Sakiyama A,Deshimaru M,Terada S

更新日期：2007-07-27 00:00:00

abstract：INTRODUCTION:SIRT2 is a NAD(+)-dependent deacetylases and associated with numerous processes such as infection, carcinogenesis, DNA damage and cell cycle regulation. However, the role of SIRT2 in inflammatory process in macrophage remains unclear. MATERIALS AND METHODS:In the present study, we have evaluated the regul...

journal_title：Biochemical and biophysical research communications

authors： Lee AS,Jung YJ,Kim D,Nguyen-Thanh T,Kang KP,Lee S,Park SK,Kim W

更新日期：2014-08-08 00:00:00

abstract：:The rfp/ret transforming gene was generated by DNA rearrangement which occurred during transfection of NIH 3T3 cells. The amino-terminal half of Rfp with a RING finger motif was fused to the truncated Ret receptor tyrosine kinase, leading to a transforming sequence, Rfp/Ret. In the present study, to elucidate the impo...

journal_title：Biochemical and biophysical research communications

authors： Hasegawa N,Iwashita T,Asai N,Murakami H,Iwata Y,Isomura T,Goto H,Hayakawa T,Takahashi M

更新日期：1996-08-14 00:00:00