当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Interactions in the active site of Deinococcus radiodurans RNA polymerase during RNA proofreading.

Interactions in the active site of Deinococcus radiodurans RNA polymerase during RNA proofreading.

Abstract：

:Co-transcriptional RNA proofreading by RNA polymerase (RNAP) is essential for accurate mRNA synthesis and reactivation of stalled transcription complexes, which can otherwise compromise genome integrity. RNAP from the stress-resistant bacterium Deinococcus radiodurans exhibits high levels of RNA cleavage in comparison with RNAP from Escherichia coli, which allows it to remove misincorporated nucleotides with high efficiency. Here, we show that the rate of RNA cleavage by D. radiodurans RNAP depends on the structure of the (mis)matched RNA 3'-nucleotide and its contacts with the active site. These interactions likely position the reactive phosphodiester bond in the cleavage-competent conformation, thus facilitating its hydrolysis catalyzed by metal ions in the active center. The universal RNA cleavage factor GreA largely alleviates defects in RNA cleavage caused by modifications in the RNA 3'-nucleotide or in its binding pocket in RNAP, suggesting that GreA functionally substitutes for these contacts. The results demonstrate that various RNAPs rely on a conserved mechanism for RNA proofreading, which can be modulated by changes in accessory parts of the active center.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Esyunina D,Kulbachinskiy A

doi

10.1016/j.bbrc.2018.12.095

subject

Has Abstract

pub_date

2019-01-29 00:00:00

pages

161-166

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(18)32744-X

journal_volume

509

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Characterization of modified proteins in plasma from a subtype of schizophrenia based on carbonyl stress: Protein carbonyl is a possible biomarker of psychiatric disorders.

    abstract：:Although it's well known that protein carbonyl (PCO) and advanced glycation end-products (AGEs) levels are elevated in plasma from patients with renal dysfunction, we recently identified patients who had no renal dysfunction but possessed high levels of plasma pentosidine (PEN), which is an AGEs, and low vitamin B6 le...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.152

    authors： Koike S,Kayama T,Arai M,Horiuchi Y,Kobori A,Miyashita M,Itokawa M,Ogasawara Y

    更新日期：2015-11-13 00:00:00

  • The N-terminal domain of α-dystroglycan, released as a 38 kDa protein, is increased in cerebrospinal fluid in patients with Lyme neuroborreliosis.

    abstract：:α-Dystroglycan is an extracellular adhesion protein that is known to interact with different ligands. The interaction is thought to stabilize the integrity of the plasma membrane. The N-terminal part of α-dystroglycan may be proteolytically processed to generate a small 38 kDa protein (α-DG-N). The physiological signi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.07.129

    authors： Hesse C,Johansson I,Mattsson N,Bremell D,Andreasson U,Halim A,Anckarsäter R,Blennow K,Anckarsäter H,Zetterberg H,Larson G,Hagberg L,Grahn A

    更新日期：2011-09-02 00:00:00

  • Microarray evaluation of EP4 receptor-mediated prostaglandin E2 suppression of 3T3-L1 adipocyte differentiation.

    abstract：:Prostaglandin E(2) (PGE(2)) has been shown to negatively regulate adipogenesis. To explore to what extent PGE(2) inhibits the differentiation of cells to adipocytes and to examine whether its effect could be due to EP4 receptor signaling, we used microarrays to analyze the gene expression profiles of 3T3-L1 cells expo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.07.194

    authors： Sugimoto Y,Tsuboi H,Okuno Y,Tamba S,Tsuchiya S,Tsujimoto G,Ichikawa A

    更新日期：2004-09-24 00:00:00

  • Dexamethasone prevents the induction by endotoxin of a nitric oxide synthase and the associated effects on vascular tone: an insight into endotoxin shock.

    abstract：:The relationship between vascular tone and the induction by endotoxin of a nitric oxide (NO) synthase was studied in vitro in rings of rat thoracic aorta. In rings with and without endothelium there was a time-dependent induction of NO synthase accompanied by both spontaneous and L-arginine-induced relaxation and by r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80068-3

    authors： Rees DD,Cellek S,Palmer RM,Moncada S

    更新日期：1990-12-14 00:00:00

  • An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+.

    abstract：:Mg2+ is essential for guanosine triphosphatase activity and plays key roles in guanine nucleotide binding and preserving the structural integrity of GTP-binding proteins. To understand the structural basis for Mg2+ function during the GDP/GTP exchange process, we determined the crystal structure of Delta9-Sar1-GDP at ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.07.148

    authors： Rao Y,Bian C,Yuan C,Li Y,Chen L,Ye X,Huang Z,Huang M

    更新日期：2006-09-29 00:00:00

  • Interpretation of field potentials measured on a multi electrode array in pharmacological toxicity screening on primary and human pluripotent stem cell-derived cardiomyocytes.

    abstract：:Multi electrode arrays (MEAs) are increasingly used to detect external field potentials in electrically active cells. Recently, in combination with cardiomyocytes derived from human (induced) pluripotent stem cells they have started to become a preferred tool to examine newly developed drugs for potential cardiac toxi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.01.151

    authors： Tertoolen LGJ,Braam SR,van Meer BJ,Passier R,Mummery CL

    更新日期：2018-03-18 00:00:00

  • Molecular cloning and functional characterization of a human scavenger receptor with C-type lectin (SRCL), a novel member of a scavenger receptor family.

    abstract：:Using a human placenta cDNA library, we cloned a novel member belonging to the scavenger receptor family. Complementary DNA of this clone encodes a type II transmembranous glycoprotein containing a collagen-like domain, which are typical structural characteristics of scavenger receptor class A. This protein also conta...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4210

    authors： Nakamura K,Funakoshi H,Miyamoto K,Tokunaga F,Nakamura T

    更新日期：2001-02-02 00:00:00

  • DNA crosslinking by 3'-(3-cyano-4-morpholinyl)-3'-deaminoadriamycin in HT-29 human colon carcinoma cells in vitro.

    abstract：:The Adriamycin analog, 3'-(3-cyano-4-morpholinyl)-3'-deaminoadriamycin, is 100- to 1000-fold more active than Adriamycin as an antitumor agent in vivo and in vitro. The interaction of 3'-(3-cyano-4-morpholinyl)-3'deaminoadriamycin and Adriamycin with DNA has been studied in HT-29 human colon carcinoma cells in vitro. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91807-8

    authors： Begleiter A,Johnston JB

    更新日期：1985-08-30 00:00:00

  • Significant correlation of the SCN1A mutations and severe myoclonic epilepsy in infancy.

    abstract：:To investigate the possible correlation between genotype and phenotype of epilepsy, we analyzed the voltage-gated sodium channel alpha1-subunit (SCN1A) gene, beta1-subunit (SCN1B) gene, and gamma-aminobutyric acid(A) receptor gamma2-subunit (GABRG2) gene in DNAs from peripheral blood cells of 29 patients with severe m...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)00617-4

    authors： Ohmori I,Ouchida M,Ohtsuka Y,Oka E,Shimizu K

    更新日期：2002-07-05 00:00:00

  • Synergistic inhibition of carboxypeptidase A by zinc ion and imidazole.

    abstract：:Zinc ion in solution yields a 560-fold enhancement in the kinetic inhibition of carboxypeptidase A by the simple heterocycle imidazole, behavior attributed to formation of a ternary complex of the three species. However, the effect is partially negated by formation of less-inhibitory Zn2+(C3H4N2)2-4 coordination compl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0440

    authors： Mock WL,Wang L

    更新日期：1999-04-02 00:00:00

  • Possible involvement of a L-delta 1-pyrroline-5-carboxylate (P5C) reductase in the synthesis of proline in Desulfovibrio desulfuricans Norway.

    abstract：:A L-delta 1-pyrroline-5-carboxylate reductase activity has been detected in crude extracts of Desulfovibrio desulfuricans Norway. This P5C reductase activity is also found when a 2.5 kb D. desulfuricans DNA fragment is introduced into an Escherichia coli proC mutant. Although it restores growth of the proC mutant, the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91931-2

    authors： Fons M,Cami B,Chippaux M

    更新日期：1991-09-16 00:00:00

  • Complex role of protein kinase C in mediating the supramaximal inhibition of pancreatic secretion observed with cholecystokinin.

    abstract：:Protein kinase C appears to play an important, yet complex role in the supramaximal inhibition of pancreatic acinar cell secretion observed in response to cholecystokinin (CCK). The addition of protein kinase C activation to the concentration-response curve of a partial agonist acting at the CCK receptor (a phenethyl ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81522-0

    authors： Gaisano HY,Miller LJ

    更新日期：1992-08-31 00:00:00

  • Modified sympathetic regulation in N-type calcium channel null-mouse.

    abstract：:To elucidate the physiological importance of neuronal (N)-type calcium channels in sympathetic controls, we analyzed N-type channel-deficient (NKO) mice. Immunoprecipitation analysis revealed increased interaction between beta3 (a major accessory subunit of N-type channels) and R-type channel-forming CaV2.3 in NKO mic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.01.087

    authors： Murakami M,Ohba T,Wu TW,Fujisawa S,Suzuki T,Takahashi Y,Takahashi E,Watanabe H,Miyoshi I,Ono K,Sasano H,Ito H,Iijima T

    更新日期：2007-03-23 00:00:00

  • Identification of genetic variation in the human serotonin 1D beta receptor gene.

    abstract：:Disturbances of serotonergic pathways have been implicated in a wide variety of neuropsychiatric disorders such as depression, anxiety, migraine, and substance abuse. Genetic variation in genes coding for serotonin receptor proteins might well be involved in the genetic predisposition to these diseases and/or of pharm...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2792

    authors： Nöthen MM,Erdmann J,Shimron-Abarbanell D,Propping P

    更新日期：1994-12-15 00:00:00

  • Vascular actions of C-type natriuretic peptide in isolated porcine coronary arteries and coronary vascular smooth muscle cells.

    abstract：:C-type natriuretic (CNP) caused concentration-dependent relaxations in porcine coronary arteries with a maximal relaxation (10(-6)M) of 46%. Relaxations to CNP in isolated coronary arteries were significantly attenuated with potassium channel antagonists charybdotoxin (10(-7)M) and glibenclamide (10(-7)M). Membrane po...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2731

    authors： Wei CM,Hu S,Miller VM,Burnett JC Jr

    更新日期：1994-11-30 00:00:00

  • Silencing Nrf2 impairs glioma cell proliferation via AMPK-activated mTOR inhibition.

    abstract：:Gliomas are the leading cause of death among adults with primary brain malignancies. Treatment for malignant gliomas remains limited, and targeted therapies have been incompletely explored. Nuclear factor erythroid 2-related factor 2 (Nrf2), a key transcription regulator for antioxidant and detoxification enzymes, is ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.12.034

    authors： Jia Y,Wang H,Wang Q,Ding H,Wu H,Pan H

    更新日期：2016-01-15 00:00:00

  • Angiotensin II inhibitory peptide found in the receptor sequence using peptide array.

    abstract：:Peptide array consisting of hundreds of peptides spatially addressed and synthesized on a cellulose membrane support was used to screen ligand-inhibitory peptides. As a model, angiotensin II (Ang II), a significant peptide related to the treatment of cardiovascular diseases, was chosen as the target ligand. Peptide ar...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.01.016

    authors： Kato R,Kunimatsu M,Fujimoto S,Kobayashi T,Honda H

    更新日期：2004-02-27 00:00:00

  • LncRNA LINC00319 accelerates ovarian cancer progression through miR-423-5p/NACC1 pathway.

    abstract：:Long noncoding RNA (lncRNA) LINC00319 has been reported to promote carcinogenesis of lung cancer and cutaneous squamous cell carcinoma. However, the role and mechanism of LINC00319 in ovarian cancer progression is unclear. In this study, LINC00319 expression was found to be upregulated in ovarian cancer tissues and ce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.11.006

    authors： Du W,Feng Z,Sun Q

    更新日期：2018-12-09 00:00:00

  • Heteroplasmy of mitochondrial genomes in clonal cultures from patients with Kearns-Sayre syndrome.

    abstract：:We have analyzed heteroplasmy of mitochondrial DNA in clonal cultures from two patients with Kearns-Sayre syndrome, and have found that individual muscle or fibroblast clones contained either a mixed (i.e. heteroplasmic) population of normal and deleted mitochondrial DNAs, or only normal mitochondrial DNAs (i.e. homop...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92499-6

    authors： Moraes CT,Schon EA,DiMauro S,Miranda AF

    更新日期：1989-04-28 00:00:00

  • Isolation of fatty acids covalently bound to the gastric mucus glycoprotein of normal and cystic fibrosis patients.

    abstract：:Covalently bound fatty acids were found in strictly purified and delipidated gastric mucus glycoprotein of normal and cystic fibrosis individuals. The susceptibility of this linkage to methanolic KOH and hydroxylamine treatment indicated the ester bond between fatty acids and glycoprotein. On the average, 2.9 nmol fat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90464-3

    authors： Slomiany A,Slomiany BL,Witas H,Aono M,Newman LJ

    更新日期：1983-05-31 00:00:00

  • Purification and molecular properties of ascorbate peroxidase from bovine eye.

    abstract：:Ascorbate peroxidase (APX) is a hydrogen peroxide-scavenging peroxidase which uses ascorbate (AsA) as the specific electron donor. APX has not been isolated in mammals. Ocular tissue contains AsA at high concentrations, and we detected APX activity in bovine retinal pigment epithelium (RPE) and choroid. We purified AP...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7946

    authors： Wada N,Kinoshita S,Matsuo M,Amako K,Miyake C,Asada K

    更新日期：1998-01-14 00:00:00

  • Cleavage-induced termination in U2 snRNA gene expression.

    abstract：:The maturation of many small nuclear RNAs is dependent on RNase III-like endonuclease mediated cleavage, which generates a loading site for the exosome complex that trims the precursor at its 3' end. Using a temperature sensitive Pac1 nuclease, here we show that the endonuclease cleavage is equally important in termin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.02.023

    authors： Nabavi S,Nazar RN

    更新日期：2010-03-12 00:00:00

  • The binding of N-(phosphonacetyl)-L-aspartate to aspartate carbamoyltransferase of Escherichia coli.

    abstract：:A more precise description of the binding of N-(phosphonacetyl)-L- aspartate to the catalytic chains of aspartate carbamoyltransferase clarifies aspects of the specificity of this enzyme toward its substrates, carbamoylphosphate and L-aspartate, and suggests a catalytic role for His-134. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90514-0

    authors： Volź KW,Krause KL,Lipscomb WN

    更新日期：1986-04-29 00:00:00

  • Amino acid sequence requirements of peptides that inhibit polyglutamine-protein aggregation and cell death.

    abstract：:Proteins with expanded polyglutamine domains cause eight inherited neurodegenerative diseases including Huntington's disease. In a previous paper, we identified peptides that inhibit polyglutamine protein aggregation and cell death and now describe the amino acid sequence requirements necessary for these activities. T...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5783

    authors： Ren H,Nagai Y,Tucker T,Strittmatter WJ,Burke JR

    更新日期：2001-11-02 00:00:00

  • Regulation of lipogenesis via BHLHB2/DEC1 and ChREBP feedback looping.

    abstract：:BHLHB2/DEC1 is a transcription factor implicated in cell proliferation, apoptosis, and metabolism, and is also known to play an important role in the regulation of the mammalian circadian rhythm. However, its precise role in metabolism remains unclear. We investigated the link between BHLHB2 and ChREBP, a glucose-acti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.06.101

    authors： Iizuka K,Horikawa Y

    更新日期：2008-09-12 00:00:00

  • p21-activated kinase PAK phosphorylates desmin at sites different from those for Rho-associated kinase.

    abstract：:p21-activated kinase (PAK) and Rho-associated kinase (Rho-kinase) have been shown to induce Ca(2+)-independent contraction of smooth muscle. PAK-induced contraction of Triton-skinned smooth muscle correlates with increased phosphorylation of caldesmon and desmin, although the role of desmin phosphorylation has remaine...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2854

    authors： Ohtakara K,Inada H,Goto H,Taki W,Manser E,Lim L,Izawa I,Inagaki M

    更新日期：2000-06-16 00:00:00

  • Expression of dopamine-associated genes on conjunctiva stromal-derived human mesenchymal stem cells.

    abstract：:Parkinson's disease (PD) is neurodegenerative diseases caused by the loss of dopaminergic neurons in the substantia nigra pars compacta. Stem cell therapy is one of the promising strategies in helping to cure PD. In the present study, human mesenchymal stem cells (MSCs) from eye conjunctiva stromal cells were differen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.09.148

    authors： Nadri S,Soleimani M,Mobarra Z,Amini S

    更新日期：2008-12-12 00:00:00

  • Polymorphisms of the D4 dopamine receptor alleles in chronic alcoholism.

    abstract：:We have screened genomic DNA for the identification of D4 dopamine receptor polymorphisms. We show that the D4 dopamine receptor genotype in 72 severely affected chronic alcoholics is heterogeneous, with individuals homozygous and heterozygous for the various D4 receptor alleles. Alcoholics demonstrated a greater prev...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2222

    authors： George SR,Cheng R,Nguyen T,Israel Y,O'Dowd BF

    更新日期：1993-10-15 00:00:00

  • Long noncoding RNA expression in dermal papilla cells contributes to hairy gene regulation.

    abstract：:Dermal papilla (DP) cells may be the source of dermal-derived signaling molecules involved in hair-follicle development and postnatal hair cycling. Early-passage DP cells can induce hair growth in vivo, but, on further culture, this ability is lost. The cellular mechanisms underlying the hair-follicle induction proper...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.09.119

    authors： Lin CM,Liu Y,Huang K,Chen XC,Cai BZ,Li HH,Yuan YP,Zhang H,Li Y

    更新日期：2014-10-24 00:00:00

  • Human kallikrein 13 involvement in extracellular matrix degradation.

    abstract：:The human kallikrein family is a group of 15 serine protease genes clustered on chromosome 19q13.4 and shares a high degree of homology. These proteolytic enzymes have diverse physiological functions in many different tissues. Growing evidence suggests that many kallikreins are differentially expressed in cancer and m...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.08.206

    authors： Kapadia C,Ghosh MC,Grass L,Diamandis EP

    更新日期：2004-10-22 00:00:00