当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Interpretation of field potentials measured on a multi electrode array in pharmacological toxicity screening on primary and human pluripotent stem cell-derived cardiomyocytes.

Interpretation of field potentials measured on a multi electrode array in pharmacological toxicity screening on primary and human pluripotent stem cell-derived cardiomyocytes.

Abstract：

:Multi electrode arrays (MEAs) are increasingly used to detect external field potentials in electrically active cells. Recently, in combination with cardiomyocytes derived from human (induced) pluripotent stem cells they have started to become a preferred tool to examine newly developed drugs for potential cardiac toxicity in pre-clinical safety pharmacology. The most important risk parameter is proarrhythmic activity in cardiomyocytes which can cause sudden cardiac death. Whilst MEAs can provide medium- to high- throughput noninvasive assay platform, the translation of a field potential to cardiac action potential (normally measured by low-throughput patch clamp) is complex so that accurate assessment of drug risk to the heart is in practice still challenging. To address this, we used computational simulation to study the theoretical relationship between aspects of the field potential and the underlying cardiac action potential. We then validated the model in both primary mouse- and human pluripotent (embryonic) stem cell-derived cardiomyocytes showing that field potentials measured in MEAs could be converted to action potentials that were essentially identical to those determined directly by electrophysiological patch clamp. The method significantly increased the amount of information that could be extracted from MEA measurements and thus combined the advantages of medium/high throughput with more informative readouts. We believe that this will benefit the analysis of drug toxicity screening of cardiomyocytes using in time and accuracy.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Tertoolen LGJ,Braam SR,van Meer BJ,Passier R,Mummery CL

doi

10.1016/j.bbrc.2017.01.151

subject

Has Abstract

pub_date

2018-03-18 00:00:00

pages

1135-1141

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(17)30219-X

journal_volume

497

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Stabilization of C-terminal binding protein 2 by cellular inhibitor of apoptosis protein 1 via BIR domains without E3 ligase activity.

    abstract：:C-terminal binding protein 2 (CtBP2) is a transcriptional co-repressor that regulates many genes involved in normal cellular events. Because CtBP2 overexpression has been implicated in various human cancers, its protein levels must be precisely regulated. Previously, we reported that CtBP1 and CtBP1-mediated transcrip...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.05.098

    authors： Seo TW,Lee YT,Lee JS,Yoo SJ

    更新日期：2020-09-17 00:00:00

  • Swings in blood glucose levels accelerate atherogenesis in apolipoprotein E-deficient mice.

    abstract：:The aim of this study was to investigate the effect of fluctuations in blood glucose levels on atherogenesis. Apolipoprotein (apo) E-deficient mice fed maltose twice daily were used as a model of repetitive postprandial glucose spikes. We investigated the number of macrophages adherent to the endothelium and the area ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.118

    authors： Mita T,Otsuka A,Azuma K,Uchida T,Ogihara T,Fujitani Y,Hirose T,Mitsumata M,Kawamori R,Watada H

    更新日期：2007-07-06 00:00:00

  • Polyunsaturated fatty acids do not activate AMP-activated protein kinase in mouse tissues.

    abstract：:Stearoyl-CoA desaturase 1 (SCD1) deficiency partitions fatty acids away from lipid synthesis towards fatty acid oxidation in liver and skeletal muscle in part due to activation of AMP-activated protein kinase (AMPK) pathway. The mechanism of AMPK activation by SCD1 mutation is unknown, however since SCD1-/- animals ha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.05.026

    authors： Dobrzyn A,Dobrzyn P,Miyazaki M,Ntambi JM

    更新日期：2005-07-08 00:00:00

  • The effect of interferon on cells deficient in nucleoside transport or lacking thymidine kinase activity.

    abstract：:Mutants of mouse T-lymphoma S49 cells lacking thymidine kinase activity or deficient in nucleoside transport were selected by growth in the presence of 5'-fluorodeoxyuridine and their sensitivity to interferon tested. All five thymidine kinase and both transport deficient mutants were sensitive to the antiproliferativ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91076-3

    authors： Gewert DR,Cohen A,Williams BR

    更新日期：1984-01-13 00:00:00

  • MicroRNA involvement in glioblastoma pathogenesis.

    abstract：:MicroRNAs are endogenously expressed regulatory noncoding RNAs. Altered expression levels of several microRNAs have been observed in glioblastomas. Functions and direct mRNA targets for these microRNAs have been relatively well studied over the last years. According to these data, it is now evident, that impairment of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2009.06.034

    authors： Novakova J,Slaby O,Vyzula R,Michalek J

    更新日期：2009-08-14 00:00:00

  • Reduced expression of INK4a/ARF genes in stem-like sphere cells from rat sarcomas.

    abstract：:The presence of cancer stem cells, in both hematopoietic and solid malignancies, has been recently linked to their pathogenesis. We aimed to identify the characteristics and stem-like properties of sphere-colony forming cells in rat osteosarcoma and malignant fibrous histiocytoma cell lines. The results showed that bo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.08.067

    authors： Fujii H,Honoki K,Tsujiuchi T,Kido A,Yoshitani K,Mori T,Takakura Y

    更新日期：2007-10-26 00:00:00

  • Involvement of a common 10-amino-acid segment in the cytoplasmic region of CD40 but different MAP kinases in different CD40-mediated responses.

    abstract：:CD40-mediated signals can induce cell aggregation, proliferation and rescue from apoptosis in WEHI231. To define which segment of cytoplasmic domain of CD40 and how signals are involved in those events, we generated mutant CD40 transfectants. We demonstrated the same 10 amino acid segment that could bind to tumor necr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6343

    authors： Hara T,Murakami M,Maeda H,Hibi M,Uede T

    更新日期：1997-04-07 00:00:00

  • Opposing roles of STAT-1 and STAT-3 in regulating vascular endothelial growth factor expression in vascular smooth muscle cells.

    abstract：:Increased microvessel density in atherosclerotic plaques plays a major role in promoting plaque destabilization resulting in increased risk of stroke and myocardial infarction. Previously we have shown that expression of the inflammatory cytokine, Oncostatin-M (OSM), in human atherosclerotic plaques correlated with in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.10.037

    authors： Albasanz-Puig A,Murray J,Namekata M,Wijelath ES

    更新日期：2012-11-09 00:00:00

  • Magnetic characterization of Daphnia resting eggs.

    abstract：:This study characterized the magnetic materials found within Daphnia resting eggs by measuring static magnetization with a superconducting quantum interference device (SQUID) magnetometer, after forming two types of conditions, each of which consists of zero-field cooling (ZFC) and field cooling (FC). Magnetic ions, s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.078

    authors： Sakata M,Kawasaki T,Shibue T,Takada A,Yoshimura H,Namiki H

    更新日期：2006-12-15 00:00:00

  • Metyrapone modulation of tyrosine aminotransferase induction by dexamethasone in cultured hepatocytes.

    abstract：:Metyrapone, an inhibitor of cytochrome P-450-dependent monooxygenases, enhanced the induction of tyrosine aminotransferase by dexamethasone in primary cultures of hepatocytes, while it had no effect on the basal level of the enzyme activity in the absence of the hormone. The amplification of the hormonal induction of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90880-v

    authors： Tongiani R,Piazzolla S,Paolicchi A

    更新日期：1990-01-30 00:00:00

  • Histone deacetylase inhibition results in decreased macrophage CD9 expression.

    abstract：:Histone deacetylase (HDAC) inhibitors have been demonstrated to regulate myeloid cell differentiation. In the present study the effects of the HDAC inhibitor trichostatin A (TSA) on the tetraspanin cell surface antigen CD9 were determined in primary murine macrophages. TSA inhibited CD9 protein and message expression ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00523-5

    authors： Wang XQ,Alfaro ML,Evans GF,Zuckerman SH

    更新日期：2002-06-14 00:00:00

  • Polyethylenimine-mediated suicide gene transfer induces a therapeutic effect for hepatocellular carcinoma in vivo by using an Epstein-Barr virus-based plasmid vector.

    abstract：:The present study aimed to establish a novel efficient nonviral strategy for suicide gene transfer in hepatocellular carcinoma (HCC) in vivo. We employed branched polyethylenimine (PEI) and combined it with Epstein-Barr virus (EBV)-based plasmid vectors. The HCC cells transfected with an EBV-based plasmid carrying the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6383

    authors： Iwai M,Harada Y,Tanaka S,Muramatsu A,Mori T,Kashima K,Imanishi J,Mazda O

    更新日期：2002-02-15 00:00:00

  • In vitro heat effect on functional and conformational changes of cyclodextrin glucanotransferase from hyperthermophilic archaea.

    abstract：:The in vitro heat effect on protein characteristics of thermostable enzyme was examined using a cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) from the hyperthermophilic archaeon Thermococcus sp. B1001 as a model protein. The recombinant form of CGTase was obtained as an inclusion body from Escherichia coli cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1629

    authors： Yamamoto T,Shiraki K,Fujiwara S,Takagi M,Fukui K,Imanaka T

    更新日期：1999-11-01 00:00:00

  • A novel marker for terminal Schwann cells, homocysteine-responsive ER-resident protein, as isolated by a single cell PCR-differential display.

    abstract：:Terminal Schwann cells (TSCs) that cover motor neuron terminals are known to play important roles in maintaining neuromuscular junctions, as well as in the repair process after nerve injury. However, molecular characteristics of TSCs remain unknown, because of the difficulties in analyzing them due to their paucity. W...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01499-2

    authors： Oda R,Yaoi T,Okajima S,Kobashi H,Kubo T,Fushiki S

    更新日期：2003-09-05 00:00:00

  • The mammalian heat shock protein binding immunophilin (p59/HBI) is an ATP and GTP binding protein.

    abstract：:A protein of M(r) 59,000 (p59) was recently cloned and identified as a Heat shock protein Binding Immunophilin (p59/HBI). It participates to the heterooligomeric, non-DNA binding form of steroid receptors, in association with the heat shock protein of M(r) 90,000 (hsp90). It binds the immunosuppressants FK506 and rapa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2088

    authors： Le Bihan S,Renoir JM,Radanyi C,Chambraud B,Joulin V,Catelli MG,Baulieu EE

    更新日期：1993-09-15 00:00:00

  • On the specificity of a bacteriophage-borne endoglycanase for the native capsular polysaccharide produced by Klebsiella pneumoniae SK1 and its derived polymers.

    abstract：:The specificity of the endoglycanase associated with the bacteriophage phi SK1 particles was tested on the native capsular polysaccharide produced by Klebsiella pneumoniae serotype SK1 and on three chemically modified polymers derived from it. The primary structure of the SK1 capsular polysaccharide is: [formula: see ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1160

    authors： Cescutti P,Paoletti S

    更新日期：1994-02-15 00:00:00

  • N-glycosylation of forms of angiotensin converting enzyme from four mammalian species.

    abstract：:To help clarify bases for the molecular weight and surface charge heterogeneities of forms of somatic angiotensin converting enzyme (ACE), we examined for differences in N-glycosylation. ACE preparations purified from human, guinea pig, rat and rabbit tissues were found to be heterogeneous in terms of numbers of N-gly...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2278

    authors： Ripka JE,Ryan JW,Valido FA,Chung AY,Peterson CM,Urry RL

    更新日期：1993-10-29 00:00:00

  • In vitro hydroxylation of bentazon by microsomes from naphthalic anhydride-treated corn shoots.

    abstract：:In vitro metabolism of the herbicide bentazon was studied in microsomal membranes isolated from 6-day-old etiolated corn shoots. Microsomes isolated from shoots of nontreated seeds did not metabolize bentazon when assayed with NADPH or peroxides. However, microsomes isolated from shoots of seeds pretreated with naphth...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91695-o

    authors： McFadden JJ,Gronwald JW,Eberlein CV

    更新日期：1990-04-16 00:00:00

  • Toosendanin induces caspase-dependent apoptosis through the p38 MAPK pathway in human gastric cancer cells.

    abstract：:Although many advances have been made in the treatment of gastric cancer (GC), numerous difficulties, such as the emergence of chemo-drug resistance, continue to lead to disappointing GC prognoses. Thus, novel alternative strategies are urgently needed. The use of natural products could be a viable option to treat GC....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.093

    authors： Zhou Q,Wu X,Wen C,Wang H,Wang H,Liu H,Peng J

    更新日期：2018-10-20 00:00:00

  • Identification and cloning of two novel allergens from the lipophilic yeast, Malassezia furfur.

    abstract：:Two novel allergens, designated Mal f 2 and Mal f 3 according to the WHO/IUIS Allergen Nomenclature Subcommittee recommendation, were isolated from the lipophilic yeast Malassezia furfur cell extracts and the genes coding for those were cloned. Mal f 2 and Mal f 3 had apparent molecular weights of 21 kDa and 20 kDa, r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8944

    authors： Yasueda H,Hashida-Okado T,Saito A,Uchida K,Kuroda M,Onishi Y,Takahashi K,Yamaguchi H,Takesako K,Akiyama K

    更新日期：1998-07-20 00:00:00

  • Involvement of calcium in the transient depolarization of E.coli cytoplasmic membrane induced by phage adsorption: a study with the fluorescent calcium indicator QUIN2.

    abstract：:Adsorption of phage T4 to its outer membrane receptor (the lipopolysaccharide) triggers a transient depolarization of the cytoplasmic membrane which is prevented upon addition of EGTA, suggesting that calcium is necessary for the transmission of the signal between the two membranes. Using the fluorescent indicator QUI...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91318-x

    authors： Boulanger P,Labedan B,Letellier L

    更新日期：1985-09-16 00:00:00

  • General order parameter based correlation analysis of protein backbone motions between experimental NMR relaxation measurements and molecular dynamics simulations.

    abstract：:Internal backbone dynamic motions are essential for different protein functions and occur on a wide range of time scales, from femtoseconds to seconds. Molecular dynamic (MD) simulations and nuclear magnetic resonance (NMR) spin relaxation measurements are valuable tools to gain access to fast (nanosecond) internal mo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.01.018

    authors： Liu Q,Shi C,Yu L,Zhang L,Xiong Y,Tian C

    更新日期：2015-02-13 00:00:00

  • BDNF induction with mild exercise in the rat hippocampus.

    abstract：:Although chronic voluntary physical activity has been shown to enhance hippocampal brain-derived neurotrophic factor (BDNF) expression in animals, the effects of forced exercise on a treadmill have not been fully investigated. We assessed induction of c-fos and BDNF expression with acute exercise at different running ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.173

    authors： Soya H,Nakamura T,Deocaris CC,Kimpara A,Iimura M,Fujikawa T,Chang H,McEwen BS,Nishijima T

    更新日期：2007-07-13 00:00:00

  • Structural studies of the SET domain from RIZ1 tumor suppressor.

    abstract：:RIZ1 is a transcriptional regulator and tumor suppressor that catalyzes methylation of lysine 9 of histone H3. It contains a distinct SET domain, sometimes referred to as PR (PRDI-BF1 and RIZ1 homology) domain, that is responsible for its catalytic activity. We determined the solution structure of the PR domain from R...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.12.034

    authors： Briknarová K,Zhou X,Satterthwait A,Hoyt DW,Ely KR,Huang S

    更新日期：2008-02-15 00:00:00

  • Cytochrome P450 and steroid hydroxylase activity in mouse olfactory and vomeronasal mucosa.

    abstract：:The aims of this study are to identify the sex steroid-metabolizing cytochrome P450 enzymes of the vomeronasal organ (VNO) and to determine the activities of VNO microsomes to metabolize estradiol, progesterone, and testosterone. Several P450 isoforms, including CYP1A2, CYP2A, CYP2B, CYP2C, CYP2G1, and CYP3A, NADPH P4...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1807

    authors： Gu J,Dudley C,Su T,Spink DC,Zhang QY,Moss RL,Ding X

    更新日期：1999-12-09 00:00:00

  • Repair of potentially lethal and sublethal damage induced by neocarzinostatin in normal and ataxia-telangiectasia skin fibroblasts.

    abstract：:Neocarzinostatin is a radiomimetic antibiotic with a potent cytotoxic effect which elicits a hypersensitive response in human cells homozygous or heterozygous for the gene for ataxia-telangiectasia. The extent and the time course of potentially lethal damage repair and sublethal damage repair following neocarzinostati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91175-0

    authors： Shiloh Y,Tabor E,Becker Y

    更新日期：1983-01-27 00:00:00

  • The IRI release from insulin gene-transduced hepatic cells responds to ambient glucose concentration.

    abstract：:Insulin gene was induced into differentiated hepatic cell line. These cells released immunoreactive insulin (IRI) of which molecular weight was greater than mature insulin. The secretion of IRI was responsive to glucose concentration and inhibited by glucose metabolism antagonist, 2-deoxy glucose (2-DOG). The mRNA of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6445

    authors： Hayashi M,Taguchi M,Fujita T

    更新日期：1997-04-17 00:00:00

  • Transcriptional induction of proto-oncogene fos by HSV-2.

    abstract：:HSV-2 induces the transcription of c-fos gene in both human (diploid) cell strains and mouse (heteroploid) cell lines. HSV-1 is incapable of such induction. The induction of transcription is rapid and transient in both cell types. The product of transcriptional induction of c-fos in mouse cells is mainly a 4.5 kb fos ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90359-7

    authors： Goswami BB

    更新日期：1987-03-30 00:00:00

  • Calmodulin antagonists inhibit formation of platelet-activating factor in stimulated human neutrophils.

    abstract：:Human polymorphonuclear leukocytes in the presence of the Ca2+ ionophore A23187 plus Ca2+ incorporated exogenously-added [3H]acetate or 1-0-[3H]-alkyl-sn-glycero-3-phosphocholine (lysoplatelet-activating factor) into platelet-activating factor (PAF,1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine). The incorporation of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91349-4

    authors： Billah MM,Siegel MI

    更新日期：1984-01-30 00:00:00

  • Epidermal growth factor inhibits both cholecystokinin octapeptide-induced inositol 1,4,5-trisphosphate production and [CA2+]i increase in rat pancreatic acinar cells.

    abstract：:We have studied the effects of epidermal growth factor (EGF) on both cholecystokinin octapeptide (CCK-OP)-induced inositol-1,4,5 trisphosphate (IP3) production and on cytosolic free calcium concentrations [Ca2+]i by fluorescence measurements in fura-2-loaded pancreatic acini. Our data show that EGF inhibits CCK-OP ind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81150-7

    authors： Pröfrock A,Piiper A,Eckhardt L,Schulz I

    更新日期：1991-10-31 00:00:00