In vitro heat effect on functional and conformational changes of cyclodextrin glucanotransferase from hyperthermophilic archaea.

abstract：:PRA1 (prenylated Rab acceptor) is a general regulator of Rab proteins, while RILP (Rab interacting lysosomal protein) is a specific effector for Rab7. It has been shown that PRA1 interacts with Rab proteins and with VAMP2. Therefore PRA1 is probably an important factor for membrane traffic, linking together the functi...

journal_title：Biochemical and biophysical research communications

authors： Bucci C,De Gregorio L,Bruni CB

更新日期：2001-08-31 00:00:00

abstract：:Drug-induced hepatotoxicity causes significant morbidity and mortality and is a major concern in drug development. This is due, in large part, to insufficient knowledge of the mechanism(s) of drug-induced liver injury. In order to address this problem, we have evaluated the modulation of gene expression within the liv...

journal_title：Biochemical and biophysical research communications

authors： Reilly TP,Bourdi M,Brady JN,Pise-Masison CA,Radonovich MF,George JW,Pohl LR

更新日期：2001-03-23 00:00:00

abstract：:The metallo-disintegrins (ADAMs) are a family of mammalian proteins with significant amino acid sequence identity and a domain organisation similar to the snake venom metalloproteinases (reprolysins). They have been implicated in a wide variety of processes such as cell-cell and cell matrix adhesion and proteolysis of...

journal_title：Biochemical and biophysical research communications

authors： Millichip MI,Dallas DJ,Wu E,Dale S,McKie N

更新日期：1998-04-17 00:00:00

abstract：:Hyaluronan synthase 2 (HAS2) is an integral membrane protein composed of multi-membrane-spanning regions and a large intracellular loop (HAS2-loop). We previously examined the effect of phorbol 12-myristate 13-acetate (PMA) and/or 4-methylumbelliferone (4-MU) on the synthesis of hyaluronan (HA) in human skin fibroblas...

journal_title：Biochemical and biophysical research communications

authors： Kasai K,Kuroda Y,Takabuchi Y,Nitta A,Kobayashi T,Nozaka H,Miura T,Nakamura T

更新日期：2020-12-17 00:00:00

abstract：:With the completion of the genome sequence of Drosophila melanogaster the importance of constructing a proteome map is to be considered. Therefore, with the application of recent advances in proteomic analysis approaches, a protein map of D. melanogaster larvae hemolymph proteins was obtained using 2-DE in the range o...

journal_title：Biochemical and biophysical research communications

authors： Guedes Sde M,Vitorino R,Tomer K,Domingues MR,Correia AJ,Amado F,Domingues P

更新日期：2003-12-19 00:00:00

abstract：:Osteoclasts which derive from hemopoietic cells are multinucleated cells responsible for bone resorption. We found that cyclopiazonic acid (CPA), thapsigargin (TG), and 2,5-di-(t-butyl)-1,4-hydroquinone (BHQ) induced osteoclast-like cell (OCL) formation in cocultures of mouse calvaria-derived stromal cells and hemopoi...

journal_title：Biochemical and biophysical research communications

authors： Takami M,Woo JT,Takahashi N,Suda T,Nagai K

更新日期：1997-08-08 00:00:00

abstract：:Translational control by specific eIF2alpha phosphorylation on serine 51 has been characterized in all eukaryotes with the significant exception of plants. In order to evaluate the capability of plant eIF2alpha to functionally control translation, the wild type (51S) and a nonphosphorylatable mutant (51A) of wheat eIF...

journal_title：Biochemical and biophysical research communications

authors： Chang LY,Yang WY,Roth D

更新日期：2000-12-20 00:00:00

abstract：:Recent studies from our laboratory demonstrated that Egr-1 is upregulated in the rat duodenal mucosa during cysteamine-induced duodenal ulceration and that antisense egr-1 oligonucleotide aggravates the duodenal ulcers. This study was aimed to determine the effects of cysteamine on redox-sensitive Egr-1 transcriptiona...

journal_title：Biochemical and biophysical research communications

authors： Khomenko T,Deng X,Jadus MR,Szabo S

更新日期：2003-10-03 00:00:00

abstract：:It has been proposed that the members of the hsp70 major heat shock protein family in non-stressed cells participate in the intracellular transport of newly synthesized proteins and in maintaining such proteins in an unfolded state. Specific binding of other cellular proteins to hsp70 may play a role in hsp70 function...

journal_title：Biochemical and biophysical research communications

authors： Margulis BA,Welsh M

更新日期：1991-07-15 00:00:00

abstract：:1,25-Dihydroxyvitamin D(3) [1,25(OH)(2) D(3)] exerts its biological effects by binding to the vitamin D receptor (VDR), which binds in turn to the vitamin D response elements located in the target gene's promoter. We have previously demonstrated that VDR binds in vitro with high affinity to the 5'-flanking sequence of...

journal_title：Biochemical and biophysical research communications

authors： Seoane S,Alonso M,Segura C,Pérez-Fernández R

更新日期：2002-03-22 00:00:00

abstract：:The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic...

journal_title：Biochemical and biophysical research communications

authors： Chao CC,Yam WC,Lin-Chao S

更新日期：1990-08-31 00:00:00

abstract：:A chemical inhibitor library of 84 compounds was screened to investigate the signaling pathway(s) leading to activation of Nrf2 in response to nitric oxide (NO). We identified the protein kinase C delta (PKCdelta) inhibitor rottlerin as the only compound that reduced NO-induced ARE-luciferase reporter activity and dim...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Hung AC,Ng PY,Nakayama K,Hu Y,Li B,Porter AG,Dhakshinamoorthy S

更新日期：2009-09-04 00:00:00

abstract：:Chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was expressed in E. coli by using a pET3a T7 RNA polymerase-based expression system and was purified to homogeneity. The kinase and bisphosphatase of the expressed bifunctional enzyme had kinetic properties identical to those of the native chicken live...

journal_title：Biochemical and biophysical research communications

authors： Li L,Yao WZ,Lange AJ,Pilkis SJ,Dong MQ,Yin Y,Xu GJ

更新日期：1995-04-26 00:00:00

abstract：:The family of G-protein-activated inward-rectifiers K+ channels presently comprise at least 3 cloned members called GIRK1, GIRK2 and GIRK3. A close structural parent of GIRK channels has recently been described as being an ATP-sensitive K+ channel. This paper shows that Xenopus expression of this new channel that we c...

journal_title：Biochemical and biophysical research communications

authors： Duprat F,Lesage F,Guillemare E,Fink M,Hugnot JP,Bigay J,Lazdunski M,Romey G,Barhanin J

更新日期：1995-07-17 00:00:00

abstract：:In this research, hemagglutinating virus of Japan envelope (HVJ-E) was used to reprogram somatic cells by fusion with mouse embryonic stem (ES) cells. Neomycin-resistant mouse embryonic fibroblasts (MEFs) were used as somatic cells. Nanog-overexpressing puromycin-resistant EB3 cells were used as mouse ES cells. These ...

journal_title：Biochemical and biophysical research communications

authors： Yue XS,Fujishiro M,Toyoda M,Akaike T,Ito Y

更新日期：2010-04-16 00:00:00

abstract：:To elucidate the role of ornithine decarboxylase (ODC) in cancer cell invasion, we have compared the invasiveness of mouse mammary carcinoma, FM3A and its variant cell line, EXOD, which overproduces ODC. We have found that EXOD cells showed about 5.6-fold more invasiveness through a reconstituted basement membrane (Ma...

journal_title：Biochemical and biophysical research communications

authors： Kubota S,Yamada T,Kamei S,Seyama Y

更新日期：1995-03-28 00:00:00

abstract：:α-Dystroglycan (α-DG) plays crucial roles in maintaining the stability of cells. We demonstrated previously that the N-terminal domain of α-DG (α-DG-N) is secreted by cultured cells into the culture medium. In the present study, to clarify its function in vivo, we generated a monoclonal antibody against α-DG-N and inv...

journal_title：Biochemical and biophysical research communications

authors： Saito F,Saito-Arai Y,Nakamura-Okuma A,Ikeda M,Hagiwara H,Masaki T,Shimizu T,Matsumura K

更新日期：2011-07-29 00:00:00

abstract：:Voltage-gated sodium channels (Na(v)) consist of a pore-forming alpha subunit (Na(v)alpha) associated with beta regulatory subunits (Na(v)beta). Adult skeletal myocytes primarily express Na(v)1.4 channels. We found, however, using neonatal L6E9 myocytes, that myofibers acquire a Na(v)1.5-cardiac-like phenotype efficie...

journal_title：Biochemical and biophysical research communications

authors： David M,Martínez-Mármol R,Gonzalez T,Felipe A,Valenzuela C

更新日期：2008-04-11 00:00:00

abstract：:Reversible protein tyrosine phosphorylation is an essential signal transduction mechanism that regulates cell growth, differentiation, mobility, metabolism, and survival. Two genes coding for protein tyrosine phophatases, designed EhPTPA and EhPTPB, were cloned from Entamoeba histolytica. EhPTPA and EhPTPB proteins sh...

journal_title：Biochemical and biophysical research communications

authors： Herrera-Rodríguez SE,Baylón-Pacheco L,Talamás-Rohana P,Rosales-Encina JL

更新日期：2006-04-21 00:00:00

abstract：:Hydrophobic modifications of transducin (T) gamma, such as farnesyl-and carboxyl-methylation, are essential for the association of T beta gamma with the photoreceptor disc membrane, and MEKA/phosducin is known to inhibit the association. In this study, we examined the effect of MEKA on the hydrophobicity of T beta gam...

journal_title：Biochemical and biophysical research communications

authors： Tanaka H,Kuo CH,Matsuda T,Fukada Y,Hayashi F,Ding Y,Irie Y,Miki N

更新日期：1996-06-25 00:00:00

abstract：:The conformations of the neuropeptide galanin in water and trifluoroethanol solutions have been examined by 1H NMR spectroscopy. Analysis of two-dimensional NMR experiments enabled the assignment of virtually all the 1H resonances of galanin in trifluoroethanol solution and many of the 1H resonances in aqueous solutio...

journal_title：Biochemical and biophysical research communications

authors： Wennerberg AB,Cooke RM,Carlquist M,Rigler R,Campbell ID

更新日期：1990-02-14 00:00:00

abstract：:Since urease of Helicobacter pylori is essential for its colonization, we focused attention on foodstuffs which inhibit the activity of this enzyme. Among plant-derived 77 foodstuff samples tested, some tea and rosemary extracts were found to clearly inhibit H. pylori urease in vitro. In particular, green tea extract ...

journal_title：Biochemical and biophysical research communications

authors： Matsubara S,Shibata H,Ishikawa F,Yokokura T,Takahashi M,Sugimura T,Wakabayashi K

更新日期：2003-10-24 00:00:00

abstract：:Pancreatic ductal carcinoma (PDC) remains one of the most intractable human malignancies. To obtain insight into the molecular pathogenesis of PDC, we constructed a retroviral cDNA expression library with total RNA isolated from the PDC cell line MiaPaCa-2. Screening of this library with the use of a focus formation a...

journal_title：Biochemical and biophysical research communications

authors： Fujiwara S,Yamashita Y,Choi YL,Wada T,Kaneda R,Takada S,Maruyama Y,Ozawa K,Mano H

更新日期：2005-12-16 00:00:00

abstract：:Topical nuclear magnetic resonance spectroscopy (NMR) using a solenoid transmit/receiver coil has been used to monitor the kinetics of intragastric utilization of [1-13C] glucose in mice in vivo. Using a double-tuned solenoid coil, signal to noise response was excellent and the natural abundance in vivo 13C spectra ob...

journal_title：Biochemical and biophysical research communications

authors： Field J,Irving MG,Brooks WM,Doddrell DM

更新日期：1984-09-28 00:00:00

abstract：:Gout patients receiving a combination of allopurinol and furosemide require higher allopurinol doses to achieve the target serum urate (SU) of <6 mg/dl (Stamp et al., 2012) [1]. Our study aimed to identify the molecular basis for this observation. We used a fluorimetric assay to determine the impact of furosemide and ...

journal_title：Biochemical and biophysical research communications

authors： Knake C,Stamp L,Bahn A

更新日期：2014-09-12 00:00:00

abstract：:The proteins belonging to SWI2/SNF2 family of DNA dependent ATPases are important members of the chromatin remodeling complexes that are implicated in epigenetic control of gene expression. We have identified a human gene with a putative DNA binding domain, which belongs to the INO80 subfamily of SWI2/SNF2 proteins. H...

journal_title：Biochemical and biophysical research communications

authors： Bakshi R,Mehta AK,Sharma R,Maiti S,Pasha S,Brahmachari V

更新日期：2006-01-06 00:00:00

abstract：:The small GTPase Rheb binds and activates mTORC1, which plays a pivotal role in diverse cellular physiologies. To increase our understanding of how Rheb regulates mTORC1 signaling, we set out to identify Rheb binding proteins using shotgun proteomics approaches. In this study, we characterized HSP70, one of the identi...

journal_title：Biochemical and biophysical research communications

authors： Ryu HH,Ha SH

更新日期：2020-12-17 00:00:00

abstract：:Recent environmental sampling around a landfill site in the UK demonstrated that unidentified xenoestrogens were present at higher levels than control sites; that these xenoestrogens were capable of super-activating (resisting ligand-dependent antagonism) the murine variant 2 ERβ and that the ionic liquid 1-octyl-3-me...

journal_title：Biochemical and biophysical research communications

authors： Leitch AC,Lakey AF,Hotham WE,Agius L,Kass GEN,Blain PG,Wright MC

更新日期：2018-09-10 00:00:00

abstract：:The exocytosis of catecholamines by chromaffin cells following stimulation (e.g. by acetylcholine) is accompanied by a rise in the level of intracellular free Ca2+. Actually, secretion can be induced merely by making the cells leaky to Ca2+ from the external medium. We have recently demonstrated that secretion can be ...

journal_title：Biochemical and biophysical research communications

authors： Harish OE,Levy R,Rosenheck K,Oplatka A

更新日期：1984-03-15 00:00:00

abstract：:Binding sites for inositol trisphosphate (IP3) have been identified in bovine adrenal cortex, employing [32P]IP3 prepared from human erythrocytes radiolabeled with [32P]ATP. IP3 was bound to adrenal microsomes with high affinity (Kd = 5 nM) and low capacity (186 fmol/mg protein). During kinetic studies, half-maximal b...

journal_title：Biochemical and biophysical research communications

authors： Baukal AJ,Guillemette G,Rubin R,Spät A,Catt KJ

更新日期：1985-12-17 00:00:00