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Characterization of a human SWI2/SNF2 like protein hINO80: demonstration of catalytic and DNA binding activity.

Abstract：

:The proteins belonging to SWI2/SNF2 family of DNA dependent ATPases are important members of the chromatin remodeling complexes that are implicated in epigenetic control of gene expression. We have identified a human gene with a putative DNA binding domain, which belongs to the INO80 subfamily of SWI2/SNF2 proteins. Here we report the cloning, expression, and functional activity of the domains from hINO80 gene both in terms of the DNA dependent ATPase as well as DNA binding activity. A differential expression of the various domains within this gene is detected in human tissues while a ubiquitous expression is detected in mice. The intranuclear localization is demonstrated using antibodies directed against the DBINO domain of hINO80.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Bakshi R,Mehta AK,Sharma R,Maiti S,Pasha S,Brahmachari V

doi

10.1016/j.bbrc.2005.10.206

subject

Has Abstract

pub_date

2006-01-06 00:00:00

pages

313-20

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)02466-6

journal_volume

339

pub_type

杂志文章

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