当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Isolation of hsp70-binding proteins from bovine muscle.

Isolation of hsp70-binding proteins from bovine muscle.

Abstract：

:It has been proposed that the members of the hsp70 major heat shock protein family in non-stressed cells participate in the intracellular transport of newly synthesized proteins and in maintaining such proteins in an unfolded state. Specific binding of other cellular proteins to hsp70 may play a role in hsp70 function. A simple method for isolation of hsp70 binding proteins using hsp70-sepharose column chromatography is described. The column binds hsp70 antibodies and thermodenatured a2-interferon in an ATP-dependent manner. Low-ionic strength extracts and actomyosin preparations from bovine muscle were passed through the column and eluates in ATP and 2M NaCl were analyzed by electrophoresis. Two proteins that eluted with ATP were identified by immunoblotting as hsp70 and actin. The polypeptides that eluted with 2 M NaCl, among which 51 and 58 kDa proteins were prominent, are suggested to bind tightly by hydrophobic interaction to hsp70. An antiserum raised against total hsp70-binding protein recognized a polypeptide with a mass of 40 kDa as determined both by immunoblotting and immunoprecipitation. Hsp70 binding proteins may thus participate in the function of hsp70 with regards to hsp70s role in protein targeting, unfolding and transport.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Margulis BA,Welsh M

doi

10.1016/0006-291x(91)91771-4

subject

Has Abstract

pub_date

1991-07-15 00:00:00

pages

1-7

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)91771-4

journal_volume

178

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Cancer cell binding to E-selectin transfected human endothelia.

    abstract：:Human endothelial cells were transiently transfected with E-Selectin which enabled us to study tumor cell/endothelial interactions following engagement of E-Selectin without the added complications of metabolic stimulation, morphological changes, and/or up regulation of other adhesion molecules due to cytokine inducti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91560-d

    authors： Merwin JR,Madri JA,Lynch M

    更新日期：1992-11-30 00:00:00

  • Copper-induced oxidation of epinephrine: protective effect of D-DAHK, a synthetic analogue of the high affinity copper binding site of human albumin.

    abstract：:Epinephrine is known to be rapidly oxidized during sepsis. Ischemia and acidosis, which often accompany sepsis, are associated with the release of weakly bound cupric ions from plasma proteins. We investigated whether copper promotes oxidation of epinephrine at both physiological and acidic pH and whether D-Asp-D-Ala-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00667-3

    authors： Roberts A,Bar-Or D,Winkler JV,Rael LT

    更新日期：2003-05-16 00:00:00

  • Inhibition of the induction of nitric oxide synthase by spermine is modulated by aldehyde dehydrogenase.

    abstract：:The observation that spermine inhibits the endotoxin (lipopolysaccharide; LPS) induced production of nitric oxide (NO) in macrophages has been ascribed to the conversion of SP to active metabolites by the action of enzymes, such as diamine oxidases, found, for example, in bovine sera. Inhibitory effect is also observe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2374

    authors： Southan GJ,Szabó C,Thiemermann C

    更新日期：1994-09-30 00:00:00

  • Cholesterol secoaldehyde induces apoptosis in J774 macrophages via mitochondrial pathway but not involving reactive oxygen species as mediators.

    abstract：:Cholesterol secoaldehyde (3beta-hydroxy-5-oxo-5,6-secocholestan-6-al or ChSeco) is an oxysterol known to be formed in reactions of ozone with cholesterol and also when cholesterol-5alpha-hydroperoxide undergoes Hock cleavage. In view of its widespread occurrence and atherogenic potential, we examined the effects of Ch...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.09.005

    authors： Gao X,Raghavamenon AC,D'Auvergne O,Uppu RM

    更新日期：2009-11-13 00:00:00

  • Cyclic AMP-specific phosphodiesterase 4 inhibitors promote ABCA1 expression and cholesterol efflux.

    abstract：:ATP cassette binding protein 1 (ABCA1) controls the apolipoprotein-mediated cholesterol efflux pathway and determines plasma HDL levels. Although cAMP is known to promote ABCA1 expression and cholesterol efflux from cells, it has not been determined whether cyclic nucleotide phosphodiesterase (PDE) isoforms regulate t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6259

    authors： Lin G,Bornfeldt KE

    更新日期：2002-01-18 00:00:00

  • Adiponectin increases bone mass by suppressing osteoclast and activating osteoblast.

    abstract：:Adiponectin, an adipose-derived hormone, exhibits various biological functions, such as increasing insulin sensitivity, protecting hypertension, and suppression of atherosclerosis, liver fibrosis, and tumor growth. Here, we report the role of adiponectin on bone metabolism. C57BL/6J mice were treated with adenovirus e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.210

    authors： Oshima K,Nampei A,Matsuda M,Iwaki M,Fukuhara A,Hashimoto J,Yoshikawa H,Shimomura I

    更新日期：2005-06-03 00:00:00

  • Flow-flash kinetics of O2 binding to cytochrome c oxidase at elevated [O2]: observations using high pressure stopped flow for gaseous reactants.

    abstract：:A high-pressure stopped-flow apparatus developed in our laboratories provides the capability to use dissolved gaseous reactants at elevated concentrations in solution (in equilibrium with gas pressures up to ca. 30 atm) for measurement of reaction kinetics. We have used this apparatus to follow the reaction of dioxyge...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0531

    authors： Bailey JA,James CA,Woodruff WH

    更新日期：1996-03-27 00:00:00

  • A plasminogen-related gene is expressed in cancer cells.

    abstract：:The breakdown of blood clots requires the fibrinolytic action of the serine proteinase plasmin, a two-chain polypeptide derived posttranslationally from its precursor zymogen, plasminogen. While investigating plasminogen gene expression in human extrahepatic tissues, a cDNA sequence was obtained which closely resemble...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)90861-e

    authors： Weissbach L,Treadwell BV

    更新日期：1992-07-31 00:00:00

  • Elevated levels of beta defensin-1 mRNA in diabetic kidneys of GK rats.

    abstract：:We previously described the isolation of CDK4, representing the 3(')end of an unknown mRNA, using differential display [Biophys. Res. Commun. 232 (1997) 49]. To determine the identity of the CDK4 gene, full-length CDK4 cDNA clones were isolated from a diabetic kidney cDNA library. DNA sequencing revealed that CDK4 rep...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.034

    authors： Page RA,Malik AN

    更新日期：2003-10-17 00:00:00

  • Effective treatment of glioblastoma requires crossing the blood-brain barrier and targeting tumors including cancer stem cells: The promise of nanomedicine.

    abstract：:Glioblastoma multiforme (GBM) is the most aggressive and lethal type of brain tumor. Both therapeutic resistance and restricted permeation of drugs across the blood-brain barrier (BBB) play a major role in the poor prognosis of GBM patients. Accumulated evidence suggests that in many human cancers, including GBM, ther...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2015.06.137

    authors： Kim SS,Harford JB,Pirollo KF,Chang EH

    更新日期：2015-12-18 00:00:00

  • Adherens junction-dependent PI3K/Akt activation induces resistance to genotoxin-induced cell death in differentiated intestinal epithelial cells.

    abstract：:The crypt-villi axis of intestinal mucosa maintains homeostasis by renewal of epithelia, and also exhibits different properties from undifferentiated to terminally differentiated cells. We investigated differential susceptibility to genotoxin-induced cell death, based on the degree of differentiation of epithelial cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.11.120

    authors： Chae B,Yang KM,Kim TI,Kim WH

    更新日期：2009-01-23 00:00:00

  • Overexpression of adenine nucleotide translocase reduces Ca2+ signal transmission between the ER and mitochondria.

    abstract：:The adenine nucleotide translocase (ANT), besides transferring ATP from the mitochondrial matrix to the rest of the cell, has also been proposed to be involved in mitochondrial permeability transition (MPT), and accordingly in mitochondrial Ca2+ homeostasis. In order to assess the role of ANT in Ca2+ signal transmissi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.07.072

    authors： Wieckowski MR,Szabadkai G,Wasilewski M,Pinton P,Duszyński J,Rizzuto R

    更新日期：2006-09-22 00:00:00

  • Implication of pH in the catalytic properties of anthrax lethal factor.

    abstract：:The anthrax lethal factor (LF) is a Zn(2+)-endopeptidase specific for mitogen-activated protein kinase kinases (MAPKKs), which are cleaved within their N-terminal region. Much line of effort was carried out to elucidate the catalytic activity of LF for designing the inhibitor and to understand the cellular mechanism o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.11.110

    authors： Kim J,Chai YG,Yoon MY

    更新日期：2004-01-02 00:00:00

  • p53 independent G(1) arrest induced by DL-alpha-difluoromethylornithine.

    abstract：:Ornithine decarboxylase (ODC), which catalyzes polyamine biosynthesis, plays an essential role in cell growth. DL-alpha-Difluoromethylornithine (DFMO), a synthetic inhibitor of ODC, inhibits cell growth. However, the exact mechanism by which polyamine depletion by DFMO results in growth inhibition remains to be elucid...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4227

    authors： Nemoto T,Kamei S,Seyama Y,Kubota S

    更新日期：2001-01-26 00:00:00

  • Expression of functional thrombin receptors in xenopus oocytes injected with human endothelial cell mRNA.

    abstract：:Human endothelial cell thrombin receptors were functionally expressed in Xenopus laevis oocytes by injection of RNA extracted from human umbilical vein endothelial cells. Oocytes injected with endothelial cell RNA responded to thrombin with a Ca2(+)-dependent depolarizing current whose size depended on the amount of R...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90770-n

    authors： Pipili-Synetos E,Gershengorn MC,Jaffe EA

    更新日期：1990-09-28 00:00:00

  • Identification of a novel murine organic anion transporter family member, OAT6, expressed in olfactory mucosa.

    abstract：:The organic anion and cation transporters (OATs and OCTs) are a large family (SLC22) of transmembrane proteins that are able to transport a variety of compounds including drugs, environmental toxins, and endogenous metabolites. OATs are expressed in various tissues, primarily kidney and liver, but also in placenta, sm...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.08.112

    authors： Monte JC,Nagle MA,Eraly SA,Nigam SK

    更新日期：2004-10-15 00:00:00

  • Reversible red-ox reactions of the diiron site in the mouse ribonucleotide reductase R2 protein.

    abstract：:The red-ox reactions of the dinuclear iron center of mouse R2 protein upon interaction with different reductants (dithionite alone and with mediators) and oxidants (PES, methylene blue, hydrogen peroxide and para-benzoquinone) have been studied by EPR and optical spectroscopy. The obtained results indicate that the tr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0207

    authors： Davydov A,Schmidt PP,Gräslund A

    更新日期：1996-02-06 00:00:00

  • Endogenous nitric oxide modulates endothelin-1 induced contraction of bovine oviduct.

    abstract：:We recently reported that oviduct epithelial cells in culture produce endothelin (ET) and postulated that ET could play an important role in the contraction of the oviduct. In the present study using an organ chamber myograph system, we evaluated the contractile effects of ET-1 on bovine oviduct ampullary-isthmus segm...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1680

    authors： Rosselli M,Imthurn B,Macas E,Keller PJ,Dubey RK

    更新日期：1994-05-30 00:00:00

  • Human ribosomal protein L7 carries two nucleic acid-binding domains with distinct specificities.

    abstract：:Protein L7 is associated with the large subunit of eukaryotic ribosomes that can act as a co-regulator of nuclear receptor-mediated transcription. In this study we show that L7 carries in addition to the known N-terminal nucleic acid-binding domain (NBD 1) a second one (NBD 2) which maps to the 50 C-terminal amino aci...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0682

    authors： von Mikecz A,Neu E,Krawinkel U,Hemmerich P

    更新日期：1999-05-19 00:00:00

  • Binding of two zinc finger nuclease monomers to two specific sites is required for effective double-strand DNA cleavage.

    abstract：:Custom-designed zinc finger nucleases (ZFNs) are becoming powerful tools in gene targeting-the process of replacing a gene within a genome by homologous recombination. Here, we have studied the DNA cleavage by one such ZFN, DeltaQNK-FN, in order to gain insight into how ZFNs cleave DNA and how two inverted sites promo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.07.021

    authors： Mani M,Smith J,Kandavelou K,Berg JM,Chandrasegaran S

    更新日期：2005-09-09 00:00:00

  • Integrative protocols for an in vitro generation of pancreatic progenitors from human dental pulp stem cells.

    abstract：:Efficiency of the induction protocol is crucial for the generation of insulin-producing cells (IPCs) from human dental pulp stem cells (hDPSCs). Here, we established the integrative induction protocol by merging genetic manipulation technique with our previous published 3-step induction protocol aiming to enhance the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.06.145

    authors： Sawangmake C,Rodprasert W,Osathanon T,Pavasant P

    更新日期：2020-09-10 00:00:00

  • Suppression of NF-κB activation by PDLIM2 restrains hepatic lipogenesis and inflammation in high fat diet induced mice.

    abstract：:Non-alcoholic fatty liver disease (NAFLD) is characterized by hepatic steatosis, insulin resistance, dyslipidemia and a systemic pro-inflammatory response, a leading cause of cirrhosis and hepatocellular carcinoma. Here, we showed that PDZ-LIM domain-containing protein 2 (PDLIM2) was an effective suppressor of steatoh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.05.187

    authors： Hao YR,Tang FJ,Zhang X,Wang H

    更新日期：2018-09-05 00:00:00

  • Sequence analysis of mouse tyrosinase cDNA and the effect of melanotropin on its gene expression.

    abstract：:Using human tyrosinase cDNA as a probe, a mouse tyrosinase cDNA clone representing approximately 75% of the tyrosinase coding region and a mouse genomic clone which includes the tyrosinase 5' coding sequences were isolated: nucleotide and deduced amino acid sequence of the mouse tyrosinase gene were determined from th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)81370-6

    authors： Kwon BS,Wakulchik M,Haq AK,Halaban R,Kestler D

    更新日期：1988-06-30 00:00:00

  • On the correlation between genomic G+C content and optimal growth temperature in prokaryotes: data quality and confounding factors.

    abstract：:The correlation between genomic G+C content and optimal growth temperature in prokaryotes has gained renewed interest after Musto et al. [H. Musto, H. Naya, A. Zavala, H. Romero, F. Alvarex-Valin, G. Bernardi, Correlations between genomic GC levels and optimal growth temperatures in prokaryotes, FEBS Lett. 573 (2004) ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2006.02.037

    authors： Wang HC,Susko E,Roger AJ

    更新日期：2006-04-14 00:00:00

  • Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase.

    abstract：:The vacuolar proton ATPase (V-ATPase) translocates protons into intracellular organelles or across the plasma membrane of specialised cells such as osteoclast and renal intercalated cells. The catalytic site of the V-ATPase consists of a hexamer of three A subunits and three B subunits which bind and hydrolyse ATP and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2434

    authors： van Hille B,Vanek M,Richener H,Green JR,Bilbe G

    更新日期：1993-11-30 00:00:00

  • Molecular tilting in dried elastoidin and its implications for the structures of other collagen fibrils.

    abstract：:Wet elastoidin spicules (fish fin rays) yield low-angle meridional X-ray diffraction patterns which resemble those from tendons. However, when the spicule dries the meridian splits into the arms of a diagonal cross (sometimes only one arm appears). Of the possible explanations we reject shearing of the axial arrangeme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(76)90229-1

    authors： Hukins DW,Woodhead-Galloway J,Knight DP

    更新日期：1976-12-20 00:00:00

  • Differential PAX3 functions in normal skin melanocytes and melanoma cells.

    abstract：:The PAX3 transcription factor is the key regulator of melanocyte development during embryogenesis and is also frequently found in melanoma cells. While PAX3 is known to regulate melanocyte differentiation, survival, proliferation and migration during development, it is not clear if its function is maintained in adult ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.07.053

    authors： Medic S,Rizos H,Ziman M

    更新日期：2011-08-12 00:00:00

  • Osterix is regulated by Erk1/2 during osteoblast differentiation.

    abstract：:Osterix (Osx) is a novel zinc finger-containing transcription factor that is essential for osteoblast differentiation and bone formation in bone homeostasis. The mitogen-activated protein (MAP) kinases are a group of evolutionarily conserved proline-directed protein serine/threonine kinases that are activated in respo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.10.097

    authors： Choi YH,Gu YM,Oh JW,Lee KY

    更新日期：2011-11-25 00:00:00

  • Species dependence of mitochondrial calcium transients during excitation-contraction coupling in isolated cardiomyocytes.

    abstract：:Whether mitochondrial Ca(2+) transport is rapid enough to respond to changes in cytosolic [Ca(2+)] ([Ca(2+)](c)) which occur during excitation-contraction coupling in the heart is controversial; different results wereobtained with different techniques and different species. In this study mitochondrial [Ca(2+)] ([Ca(2+...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1311

    authors： Griffiths EJ

    更新日期：1999-09-24 00:00:00

  • MicroRNA-613 represses prostate cancer cell proliferation and invasion through targeting Frizzled7.

    abstract：:A growing number of studies have indicated that microRNAs (miRNAs) are critical regulators of carcinogenesis and cancer progression and may serve as potential therapeutic tools for cancer therapy. Frizzled7 (Fzd7), the most important receptor of the Wnt signaling pathway, is extensively involved in cancer development ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.12.054

    authors： Ren W,Li C,Duan W,Du S,Yang F,Zhou J,Xing J

    更新日期：2016-01-15 00:00:00