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Expression of functional thrombin receptors in xenopus oocytes injected with human endothelial cell mRNA.

Abstract：

:Human endothelial cell thrombin receptors were functionally expressed in Xenopus laevis oocytes by injection of RNA extracted from human umbilical vein endothelial cells. Oocytes injected with endothelial cell RNA responded to thrombin with a Ca2(+)-dependent depolarizing current whose size depended on the amount of RNA injected. In oocytes expressing thrombin receptors, thrombin caused homologous but not heterologous desensitization. Both the catalytic and anion-binding exosites of thrombin were necessary to elicit depolarizing currents. Thus, Xenopus laevis oocytes injected with mRNA from human endothelial cells express Ca2(+)-dependent thrombin receptors which share many common features with thrombin receptors on intact endothelial cells. Xenopus oocytes may, therefore, be used as a screening system in the expression cloning of the endothelial cell thrombin receptor.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Pipili-Synetos E,Gershengorn MC,Jaffe EA

doi

10.1016/0006-291x(90)90770-n

subject

Has Abstract

pub_date

1990-09-28 00:00:00

pages

913-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(90)90770-N

journal_volume

171

pub_type

杂志文章

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