Abstract:
:Insulin gene was induced into differentiated hepatic cell line. These cells released immunoreactive insulin (IRI) of which molecular weight was greater than mature insulin. The secretion of IRI was responsive to glucose concentration and inhibited by glucose metabolism antagonist, 2-deoxy glucose (2-DOG). The mRNA of GLUT2 and glucokinase (GK) were not detected in these cells by Northern blotting. The glucose metabolism process is supposed to play an important role in the glucose responsive IRI release. It is assumed that hepatic cells have metabolic "glucose sensor", and insulin gene transduction to hepatic cells can be a physiological way of gene therapy for IDDM.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Hayashi M,Taguchi M,Fujita Tdoi
10.1006/bbrc.1997.6445subject
Has Abstractpub_date
1997-04-17 00:00:00pages
470-5issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(97)96445-7journal_volume
233pub_type
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