An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+.

abstract：:Myeloid cell leukemia-1 (Mcl-1) plays an important role in survival, chemo- and radioresistance of head and neck squamous cell carcinoma (HNSCC). Cyclin-dependent kinase 9/cyclin T (CDK9) promotes excessive production of multiple pro-survival proteins including Mcl-1, leading to impaired apoptosis of cancer cells. As ...

journal_title：Biochemical and biophysical research communications

authors： Cao S,Yu Y,Chen S,Lei D,Wang S,Pan X,Peng J

更新日期：2017-01-22 00:00:00

abstract：:AMP-activated protein kinase (AMPK) is a key regulator of energy homeostasis and its activation during T cell receptor stimulation has recently been reported. In this study, we examined the role of AMPK in interleukin (IL)-2 production in T cells. Inhibition of AMPK by compound C, a specific inhibitor of AMPK or small...

journal_title：Biochemical and biophysical research communications

authors： Jhun BS,Lee JY,Oh YT,Lee JH,Choe W,Baik HH,Kim SS,Yoon KS,Ha J,Kang I

更新日期：2006-12-29 00:00:00

abstract：:The initiating factors in primary, idiopathic osteoarthritis are unknown, the characteristic bone and cartilage changes being late features of the disease. We have proposed that biochemical cruciate ligament alteration may be important in early osteoarthritis by mediating loading consequences on the bone and cartilage...

journal_title：Biochemical and biophysical research communications

authors： Anderson-MacKenzie JM,Billingham ME,Bailey AJ

更新日期：1999-05-19 00:00:00

abstract：:Since estrogens strongly suppress the expression of alpha 2u-globulin genes in the rat liver, we studied the binding of estrogen-receptor complexes to fragments derived from alpha 2u-globulin gene RAO 01 using a DNA-cellulose competition assay. Rat uterus cytosol labelled with [3H]estradiol was used as a source of the...

journal_title：Biochemical and biophysical research communications

authors： Van Dijck P,Verhoeven G

更新日期：1992-01-15 00:00:00

abstract：:The characterization of a 20 kilodalton (20 kD) variant of rat growth hormone is reported. The 20 kD variant from rat pituitary gland extracts was identified on Western immunoblots of polyacrylamide gels. It was also shown that pituitary tissue maintained in culture secretes the 20 kD form. A rat growth hormone cDNA f...

journal_title：Biochemical and biophysical research communications

authors： Howland DS,Farrington MA,Taylor WD,Hymer WC

更新日期：1987-09-15 00:00:00

abstract：:Hepatitis C virus (HCV) affects 2-3% of the global population. Approximately one-quarter of acute infections cause chronic hepatitis that leads to liver cirrhosis or hepatocellular carcinoma. The major obstacle of current research is the extremely narrow host tropism of HCV. A single HCV strain can replicate in the Hu...

journal_title：Biochemical and biophysical research communications

authors： Lee U,Nam YR,Ye JS,Lee KJ,Kim N,Joo CH

更新日期：2014-07-25 00:00:00

abstract：:Ultraweak luminescence (uwCL) was coupled with electron spin resonance to study the reactions of 3 heterocyclic compounds (tryptophan, serotonin and imidazole) with peroxynitrite at pH 8.7. Tryptophan and serotonin reacted with emission of a flash peak of light (5 s) followed by a long-living light emission of +/- 80 ...

journal_title：Biochemical and biophysical research communications

authors： Mouithys-Mickalad A,Kohnen S,Deby C,Noels AF,Lamy M,Deby-Dupont G

更新日期：1999-06-07 00:00:00

abstract：:Pulse labeling studies with Bacillus subtilis showed that DuP 721 inhibited protein synthesis. The IC50 of DuP 721 for protein synthesis was 0.25 micrograms/ml but it was greater than 32 micrograms/ml for RNA and DNA synthesis. In cell-free systems, DuP 721 concentrations up to 100 microM did not inhibit peptide chain...

journal_title：Biochemical and biophysical research communications

authors： Eustice DC,Feldman PA,Slee AM

更新日期：1988-02-15 00:00:00

abstract：:Genomic DNA of the phytopathogenic Erwinia chrysanthemi PY35 was partially digested with Sau3AI, ligated into the BamHI site of pBluescript II SK+, and introduced into E. coli. One clone that was able to hydrolyse carboxymethylcellulose and polygalacturonic acid was selected. A 2.9 kb fragment containing the pelL1 gen...

journal_title：Biochemical and biophysical research communications

authors： Park SR,Kim MK,Kim JO,Bae DW,Cho SJ,Cho YU,Yun HD

更新日期：2000-02-16 00:00:00

abstract：:Glycosylation mutants of chinese hamster ovary cells were used to analyse the role played by surface-exposed carbohydrates on the process of interaction of Trypanosoma cruzi with the host cell. Adhesion and invasion of the parasites were markedly reduced in cells which express very few sialic acid residues. Infection ...

journal_title：Biochemical and biophysical research communications

authors： Ciavaglia Mdo C,de Carvalho TU,de Souza W

更新日期：1993-06-15 00:00:00

abstract：:In epithelial cells progression through the G1 phase of the cell cycle and preparing the cell for the S phase is regulated by cyclin D1-cdk4. Cells that express the retinoblastoma protein (pRb) are dependent on cyclin D1-cdk4 activity for their proliferation while cells that do not express pRb are not. Overexpression ...

journal_title：Biochemical and biophysical research communications

authors： Kent LL,Hull-Campbell NE,Lau T,Wu JC,Thompson SA,Nori M

更新日期：1999-07-14 00:00:00

abstract：:We have obtained the optimized geometrical structure of 2-(1-carboxy-1-hydroxyethyl)-3,4-dimethylthiazolium dipolar ion and investigated its geometric and electric changes during decarboxylation process by the MNDO-PM3 method, a molecular orbital method. The salient features of the optimized structure are that the dih...

journal_title：Biochemical and biophysical research communications

authors： Nakajima Y,Sakagishi Y,Shiibashi M,Suzuki Y,Kato H

更新日期：1993-08-31 00:00:00

abstract：:Hepatocyte growth factor, which is now known to be the same protein as scatter factor, induced oligonucleosomal fragmentation of nuclear DNA of Sarcoma 180 cells and increased the activity of caspase-3, a key component in control of the apoptotic cell death pathway to about 2.6 times that in control cells on 48 hr inc...

journal_title：Biochemical and biophysical research communications

authors： Arakaki N,Kazi JA,Kazihara T,Ohnishi T,Daikuhara Y

更新日期：1998-04-07 00:00:00

abstract：:The splicing of 3.36kb mouse mitochondrial mRNA precursor(L-strand) to 69bp RNA component is shown to be mediated by an endoribonuclease activity associated with Lettre Ehrlich ascites tumor cell mitochondria. The enzymic activity was partially purified by column chromatography and the in vitro reconstituted RNA proce...

journal_title：Biochemical and biophysical research communications

authors： Bhattacharya P

更新日期：1993-10-15 00:00:00

abstract：:CDglyTK fusion suicide gene has been well characterized to effectively kill tumor cells. However, the exact mechanism and downstream target genes are not fully understood. In our study, we found that CDglyTK/prodrug treatment works more efficiently in p53 wild-type (HONE1) cells than in p53 mutant (CNE1) cells. We the...

journal_title：Biochemical and biophysical research communications

authors： Yu Z,Wang H,Zhang L,Tang A,Zhai Q,Wen J,Yao L,Li P

更新日期：2009-09-04 00:00:00

abstract：:Catalytic activities of cytochrome P450 2B4 lacking NH2-terminal amino acids 2-27 (wt Delta2B4) and that of truncated 2B4 containing a Pro to Ser mutation at position 221 were examined in a system supported by cumene hydroperoxide. Demethylation activities of either truncated 2B4 with N-methylaniline, N,N-dimethylanil...

journal_title：Biochemical and biophysical research communications

authors： Zhang Y,Pernecky SJ

更新日期：1999-04-29 00:00:00

abstract：:We investigated the influence of the relative abundance of insulin and IGF-1 receptors on cellular growth, by measuring the stimulation of c-fos expression and of H3-thymidine incorporation into DNA by insulin and IGF-1 in CHO cells overexpressing insulin Receptor (CHO-IR). We found that CHO-IR cells were resistant to...

journal_title：Biochemical and biophysical research communications

authors： Maggi D,Laurino C,Andraghetti G,Cordera R

更新日期：1994-11-30 00:00:00

abstract：:Oncogenic osteomalacia (OOM), X-linked hypophosphatemia (XLH), and autosomal dominant hypophosphatemic rickets (ADHR) are phenotypically similar disorders characterized by hypophosphatemia, decreased renal phosphate reabsorption, normal or low serum calcitriol concentrations, normal serum concentrations of calcium and...

journal_title：Biochemical and biophysical research communications

authors： Bowe AE,Finnegan R,Jan de Beur SM,Cho J,Levine MA,Kumar R,Schiavi SC

更新日期：2001-06-22 00:00:00

abstract：:l-Aspartyl (l-Asp) and l-asparaginyl residues in proteins isomerize or racemize to d,l-isoaspartyl (d,l-isoAsp) or d-aspartyl (d-Asp) residues during protein aging. These atypical aspartyl residues can interfere with the biological function of the protein and lead to cellular dysfunction. Protein l-isoaspartyl (d-aspa...

journal_title：Biochemical and biophysical research communications

authors： Kosugi S,Furuchi T,Katane M,Sekine M,Shirasawa T,Homma H

更新日期：2008-06-20 00:00:00

abstract：:We investigated the serial expression of c-myc oncogene during differentiation of normal human burst-forming unit, erythroid (BFU-E), in vitro, together with the replating efficiency and the expression of transferrin receptors. We found that the c-myc oncogene was expressed in the early progeny of BFU-E and that this ...

journal_title：Biochemical and biophysical research communications

authors： Umemura T,Umene K,Nishimura J,Fukumaki Y,Sakaki Y,Ibayashi H

更新日期：1986-03-13 00:00:00

abstract：:Mesenchymal Stem Cells are known to engraft and integrate into the architecture of colorectal tumours, with little known regarding their fate following engraftment. This study aimed to investigate mediators of Mesenchymal Stem Cell (MSC) and colon cancer cell (CCC) interactions. Mesenchymal Stem Cells and colon cancer...

journal_title：Biochemical and biophysical research communications

authors： Hogan NM,Joyce MR,Murphy JM,Barry FP,O'Brien T,Kerin MJ,Dwyer RM

更新日期：2013-06-14 00:00:00

abstract：:Pyruvate carboxylase (PC) catalyzes the first committed step in gluconeogenesis. Here we investigated the effect of various hormones including cAMP, dexamethasone and insulin on the abundance of PC mRNA in the human hepatocyte cell line, HepG2. Treatment of HepG2 cells with 1 microM of glucagon increased the expressio...

journal_title：Biochemical and biophysical research communications

authors： Thonpho A,Sereeruk C,Rojvirat P,Jitrapakdee S

更新日期：2010-03-19 00:00:00

abstract：:The novel synthetic retinoid, CD437, shows potent anti-tumor activity in a range of different cancer cell lines and now serves as a prototype for development of new retinoid related molecules (RRMs). The purpose of this study was to examine the effect and cellular targets of CD437 in the human metastatic melanoma cell...

journal_title：Biochemical and biophysical research communications

authors： Magnussen GI,Ree Rosnes AK,Shahzidi S,Dong HP,Emilsen E,Engesæter B,Flørenes VA

更新日期：2012-04-13 00:00:00

abstract：:CPI-17 is a protein phosphatase 1 (PP1) inhibitor that has been shown to act on the myosin light chain phosphatase. CPI-17 is phosphorylated on Thr-38 in vivo, thus enhancing its ability to inhibit PP1. Thr-38 has been shown to be the target of several protein kinases in vitro. Originally, the expression of CPI-17 was...

journal_title：Biochemical and biophysical research communications

authors： Dubois T,Howell S,Zemlickova E,Learmonth M,Cronshaw A,Aitken A

更新日期：2003-03-07 00:00:00

abstract：:Skeletal muscle undergoes complicated differentiation steps that include cell-cycle arrest, cell fusion, and maturation, which are controlled through sequential expression of transcription factors. During muscle differentiation, remodeling of the epigenetic landscape is also known to take place on a large scale, deter...

journal_title：Biochemical and biophysical research communications

authors： Watanabe TM,Higuchi S,Kawauchi K,Tsukasaki Y,Ichimura T,Fujita H

更新日期：2012-02-24 00:00:00

abstract：:Notch signaling is essential for cell-fate specification in metazoans, and dysregulation of the pathway leads to a variety of human diseases including heart and vascular defects as well as cancer. Glycosylation of the Notch extracellular domain has emerged as an elegant means for regulating Notch activity, especially ...

journal_title：Biochemical and biophysical research communications

authors： Takeuchi H,Haltiwanger RS

更新日期：2014-10-17 00:00:00

abstract：:We have identified a 35-amino acid antibiotic cecropin secreted by an established mosquito cell line. C7-10 cells from the vector mosquito, Aedes albopictus, were incubated with heat-killed Escherichia coli, and materials secreted into the cell culture supernatant were recovered by acid precipitation. Following batch ...

journal_title：Biochemical and biophysical research communications

authors： Sun D,Eccleston ED,Fallon AM

更新日期：1998-08-19 00:00:00

abstract：:The net content of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] was measured in bradykinin (BK)-stimulated NIH3T3 fibroblasts and neuroblastoma-glioma hybrid cells (NG108-15). BK-mediated production of Ins(1,4,5)P3 was not affected by replacing the medium with Ca2+-free medium, but addition of EGTA (1mM) to Ca2+-free m...

journal_title：Biochemical and biophysical research communications

authors： Fu T,Okano Y,Nozawa Y

更新日期：1988-12-30 00:00:00

abstract：:The ATP-sensitive K channel (K-ATP channel) in pancreatic beta cells is believed to play a crucial role in glucose-stimulated insulin release. We investigated whether defects in the recently cloned gene for a putative subunit of this channel (KATP-2) could be a cause of diabetes in Japanese patients. The coding region...

journal_title：Biochemical and biophysical research communications

authors： Yasuda K,Sakura H,Mori Y,Iwamoto K,Shimokawa K,Kadowaki H,Hagura R,Akanuma Y,Adelman JP,Yazaki Y

更新日期：1995-06-26 00:00:00

abstract：:Glycogen storage disease type Ia (GSD-Ia) is caused by a deficiency in glucose-6-phosphatase-α (G6Pase-α or G6PC), a key enzyme in endogenous glucose production. This autosomal recessive disorder is characterized by impaired glucose homeostasis and long-term complications of hepatocellular adenoma/carcinoma (HCA/HCC)....

journal_title：Biochemical and biophysical research communications

authors： Cho JH,Kim GY,Mansfield BC,Chou JY

更新日期：2018-04-15 00:00:00