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Alternative splicing model for the synthesis and secretion of the 20 kilodalton form of rat growth hormone.

Abstract：

:The characterization of a 20 kilodalton (20 kD) variant of rat growth hormone is reported. The 20 kD variant from rat pituitary gland extracts was identified on Western immunoblots of polyacrylamide gels. It was also shown that pituitary tissue maintained in culture secretes the 20 kD form. A rat growth hormone cDNA fragment was used as a probe in S1 nuclease mapping experiments of rat pituitary poly (A) mRNA to detect the presence of two growth hormone mRNAs in the rat pituitary gland. The protected mRNAs correspond to the predicted sizes that would encode the 22 kD and 20 kD forms of growth hormone. The site of variation between the mRNAs maps to a potential alternative 3' splice site in the 5' end of exon 3 of the coding sequence. The results support the hypothesis that the 20 kD variant in rat is the product of an mRNA alternatively spliced in exon 3, as is the case for the human growth hormone.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Howland DS,Farrington MA,Taylor WD,Hymer WC

doi

10.1016/0006-291x(87)90980-6

subject

Has Abstract

pub_date

1987-09-15 00:00:00

pages

650-7

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(87)90980-6

journal_volume

147

pub_type

杂志文章

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