2',3'-Dideoxynucleoside phosphorylation by deoxycytidine kinase from normal human thymus extracts: activation of potential drugs for AIDS therapy.

abstract：:Up to now, all reported Ca(2+)-regulated photoproteins, except for mnemiopsin, have been cloned and expressed in Escherichia coli. In this study, the cDNA for an isotype of mnemiopsin, from the ctenophore Mnemiopsis leidyi, has been cloned, sequenced, and functionally expressed. The full length cDNA encoding mnemiopsi...

journal_title：Biochemical and biophysical research communications

authors： Jafarian V,Sariri R,Hosseinkhani S,Aghamaali MR,Sajedi RH,Taghdir M,Hassannia S

更新日期：2011-09-23 00:00:00

abstract：:The present study aimed to establish a novel efficient nonviral strategy for suicide gene transfer in hepatocellular carcinoma (HCC) in vivo. We employed branched polyethylenimine (PEI) and combined it with Epstein-Barr virus (EBV)-based plasmid vectors. The HCC cells transfected with an EBV-based plasmid carrying the...

journal_title：Biochemical and biophysical research communications

authors： Iwai M,Harada Y,Tanaka S,Muramatsu A,Mori T,Kashima K,Imanishi J,Mazda O

更新日期：2002-02-15 00:00:00

abstract：:Alzheimer's disease is a growing concern in the context of the increasing lifespan of the populations. The work presented here is part of the fight against this threat. It supports a therapeutic approach to reduce the incidence of Alzheimer's disease, taking advantage of the specific binding of several domains of Netr...

journal_title：Biochemical and biophysical research communications

authors： Borel F,Marzocca F,Delcros JG,Rama N,Mehlen P,Ferrer JL

更新日期：2017-07-01 00:00:00

abstract：:C(2)-ceramide, a cell-permeable analog of ceramide, caused cell death in cultured rat cortical neuronal cells. C(2)-ceramide-induced neuronal loss was accompanied by upregulation of caspase-3 activity, measured by cleavage of its fluorogenic substrate Ac-DEVD-AMC. Similar results were obtained when cortical neuronal c...

journal_title：Biochemical and biophysical research communications

authors： Movsesyan VA,Yakovlev AG,Dabaghyan EA,Stoica BA,Faden AI

更新日期：2002-11-29 00:00:00

abstract：:MitoNEET is the first identified iron sulfur protein that located in the mitochondrial outer membrane. We showed that knockdown of mitoNEET did not affect the iron sulfur protein expression in mitochondria and cytoplasm, but significantly reduced the cytosolic aconitase activity. The reduction of aconitase activity wa...

journal_title：Biochemical and biophysical research communications

authors： Tan G,Liu D,Pan F,Zhao J,Li T,Ma Y,Shen B,Lyu J

更新日期：2016-01-29 00:00:00

abstract：:Three metallo forms of peptide deformylase (PDF, EC 3.5.1.31) of Escherichia coli were prepared and crystallized (space group C2, diffraction limit 1.9 A) for initiating the X-ray structure determination of the metal center in correlation with the catalytic functionality of this enzyme. The native Fe2+ containing enzy...

journal_title：Biochemical and biophysical research communications

authors： Groche D,Becker A,Schlichting I,Kabsch W,Schultz S,Wagner AF

更新日期：1998-05-19 00:00:00

abstract：:The human dopamine D4 receptor gene (DRD4) is an important candidate gene for schizophrenia. We identified a novel -521C>T polymorphism in the 5'-promoter region of DRD4. A transient expression method revealed that the T allele of this polymorphism reduces the transcriptional efficiency by 40% compared with the C alle...

journal_title：Biochemical and biophysical research communications

authors： Okuyama Y,Ishiguro H,Toru M,Arinami T

更新日期：1999-05-10 00:00:00

abstract：:Recombinant human leukocyte interferon (IFN-alpha A) inhibits growth of the human promyelocytic leukemic cell line HL-60 without inducing these cells to differentiate terminally. When IFN-alpha A is combined with agents capable of inducing differentiation in HL-60 cells, such as 12-O-tetradecanoyl-phorbol-13-acetate (...

journal_title：Biochemical and biophysical research communications

authors： Grant S,Bhalla K,Weinstein B,Pestka S,Mileno MD,Fisher PB

更新日期：1985-07-16 00:00:00

abstract：:Glutamate receptor delta2 (GluD2) is selectively expressed on the postsynaptic spines at parallel-fiber (PF)-Purkinje neuron (PN) synapses. GluD2 knockout mice show a reduced number of PF-PN synapses, suggesting that GluD2 is involved in synapse formation. Recent studies revealed that GluD2 induces presynaptic differe...

journal_title：Biochemical and biophysical research communications

authors： Kuroyanagi T,Hirano T

更新日期：2010-07-30 00:00:00

abstract：:Reactive oxygen species (ROS) are highly reactive and directly attack surrounding biomolecules to deteriorate cellular and tissue functions. Meanwhile, ROS also serve as signaling mediators to upregulate pro-inflammatory cytokine expression via activation of the nuclear factor kappa B signaling pathway, and the increa...

journal_title：Biochemical and biophysical research communications

authors： Hu D,Li D,Shigeta M,Ochi Y,Okauchi T,Neyama H,Kabayama S,Watanabe Y,Cui Y

更新日期：2021-01-08 00:00:00

abstract：:The acute effects of 1,25-Dihydroxy-vitamin D3 [1,25(OH)2D3] on the concentration of cytoplasmic ionized calcium [Ca2+] of cultured rat mesangial cells were studied at the single cell level by microspectrofluorometry of fura-2-loaded cells. Addition of 1,25(OH)2D3 produced an immediate increase of [Ca2]+. This rise in...

journal_title：Biochemical and biophysical research communications

authors： Boland RL,de Boland AR,Nobiling R,Weinreich T,Ritz E

更新日期：1991-10-31 00:00:00

abstract：:Casper/c-FLIP is a caspase-8-related molecule critically involved in regulation of death receptor-induced apoptosis. It has been shown that Casper can either promote or antagonize apoptosis and can activate the transcription factor NF-kappaB. The exact functions of Casper are controversial. To further understand how C...

journal_title：Biochemical and biophysical research communications

authors： Li Z,Zhang J,Chen D,Shu HB

更新日期：2003-10-03 00:00:00

abstract：:A complete understanding of bitterness perception requires identification of cognate bitter substances for all human bitter taste receptors (TAS2Rs). However, so far, no agonists have been identified for five of the 25 TAS2Rs, i.e., TAS2R41, TAS2R42, TAS2R45, TAS2R48 and TAS2R60. Due to substantial genetic variability...

journal_title：Biochemical and biophysical research communications

authors： Thalmann S,Behrens M,Meyerhof W

更新日期：2013-05-31 00:00:00

abstract：:Two genomic clones containing the 3'-end of CYP3A7, the whole 5'-flanking region plus the first exon of CYP3A5 were isolated from two different human genomic banks and sequenced. The 5'-flanking region of CYP3A5, from nucleotide -1 to -1434, was 60% and 59% similar with the corresponding regions of CYP3A4 and CYP3A7, ...

journal_title：Biochemical and biophysical research communications

authors： Jounaïdi Y,Guzelian PS,Maurel P,Vilarem MJ

更新日期：1994-12-30 00:00:00

abstract：:Osterix (Osx) has been shown to be an osteoblast-specific transcription factor for bone formation. Recently, it has been reported that Osx is significantly expressed in the mouse olfactory bulb, proving that Osx may play a role in olfactory bulb development, as well as bone development. Here, we studied morphological ...

journal_title：Biochemical and biophysical research communications

authors： Park JS,Park GI,Kim JE

更新日期：2016-09-09 00:00:00

abstract：:Using a human placenta cDNA library, we cloned a novel member belonging to the scavenger receptor family. Complementary DNA of this clone encodes a type II transmembranous glycoprotein containing a collagen-like domain, which are typical structural characteristics of scavenger receptor class A. This protein also conta...

journal_title：Biochemical and biophysical research communications

authors： Nakamura K,Funakoshi H,Miyamoto K,Tokunaga F,Nakamura T

更新日期：2001-02-02 00:00:00

abstract：:We investigated whether thrombin, the final activator of coagulation cascade, regulates expression of matrix metalloproteinases (MMP)-9 in human monocytes. We show that thrombin stimulation induced MMP-9 secretion of monocytes dose- and time-dependently as revealed by gelatin zymography. Real-time RT-PCR and Western b...

journal_title：Biochemical and biophysical research communications

authors： Chang CJ,Hsu LA,Ko YH,Chen PL,Chuang YT,Lin CY,Liao CH,Pang JH

更新日期：2009-07-24 00:00:00

abstract：:The correlation between the increase of [Ca2+]i and the activation of hydrolysis of phosphoinositide-4,5-bisphosphate and formation of inositol(1,4,5)trisphosphate in neutrophils treated with Fc receptor-binding agonists is still under discussion. In this communication evidence is presented supporting the conclusion t...

journal_title：Biochemical and biophysical research communications

authors： Della Bianca V,Grzeskowiak M,Dusi S,Rossi F

更新日期：1993-11-15 00:00:00

abstract：:Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 microM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within ...

journal_title：Biochemical and biophysical research communications

authors： Grubb DR,Wolvetang EJ,Lawen A

更新日期：1995-10-24 00:00:00

abstract：:Murine interleukin 6 (mIL-6) has been synthesized as a fusion protein using a lac operon inducible plasmid in Escherichia coli. The first 8 amino acids are from the N-terminus of bacterial beta-galactosidase and the last 175 amino acids are from residue number 12 to the end of native mIL-6. This fusion protein is equi...

journal_title：Biochemical and biophysical research communications

authors： Simpson RJ,Moritz RL,Van Roost,Van Snick J

更新日期：1988-11-30 00:00:00

abstract：:Infrared spectra of E. coli strain JM103 and transformants which overproduced recombinant proinsulin have been measured as a function of pressure up to 38 kbar. It is the first time that high-pressure infrared spectra of live bacteria have been successfully measured. In ambient conditions, spectra of the host strain J...

journal_title：Biochemical and biophysical research communications

authors： Wong PT,Zahab DM,Narang SA,Sung WL

更新日期：1987-07-15 00:00:00

abstract：:Adhesive ascidians have caused serious biofouling problems and huge economic losses in marine ecosystems. However, adhesion mechanisms, particularly on functional proteins involved in ascidian adhesion, remain largely unexplored. Here, we identified 26 representative stolon proteins from the highly invasive fouling as...

journal_title：Biochemical and biophysical research communications

authors： Li S,Huang X,Chen Y,Li X,Zhan A

更新日期：2019-02-26 00:00:00

abstract：:The thromboxane receptor has two alternatively spliced isoforms, alpha and beta, which differ only in sequences within the cytoplasmic C-terminal domain. Oxidative stress induced by H(2)O(2) in a COS-7 cell model results in stabilization of the thromboxane receptor beta isoform by translocation from the endoplasmic re...

journal_title：Biochemical and biophysical research communications

authors： Valentin F,Tippins JR,Field MC

更新日期：2005-04-15 00:00:00

abstract：:Human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) plays a central role in the virus replication cycle. We found that HIV-1 RT was rapidly degraded when incubated with cell extracts obtained from human peripheral blood cells. The proteolytic activity responsible for the in vitro degradation of RT w...

journal_title：Biochemical and biophysical research communications

authors： Château MT,Robert-Hebmann V,Devaux C,Lazaro JB,Canard B,Coux O

更新日期：2001-07-27 00:00:00

abstract：:Increasing evidences suggest that, after neuregulin (NRG) stimulation, ErbB4 undergoes a series of proteolysis, including gamma-secretase cleavage. The released ErbB4 intracellular domain (EICD) is translocated into nucleus and has a transcriptional function. Although NRG-ErbB4 signaling mediates maturation of oligode...

journal_title：Biochemical and biophysical research communications

authors： Lai C,Feng L

更新日期：2004-02-06 00:00:00

abstract：:IL-1 alpha cDNA clone was isolated from a T cell line infected by the human T lymphotropic retrovirus type-I (HTLV-I/ATLV). We found significant amounts of mRNA hybridizing to IL-1 alpha cDNA not only in HTLV-I-transformed T cells but also in Epstein-Barr Virus-transformed B cells. A part of IL-2 receptor inducing act...

journal_title：Biochemical and biophysical research communications

authors： Noma T,Nakamura T,Maeda M,Okada M,Taniguchi Y,Tagaya Y,Yaoita Y,Yodoi J,Honjo T

更新日期：1986-08-29 00:00:00

abstract：:MicroRNAs (miRNAs) play crucial roles in the pancreatic carcinogenesis and progression. In the present study, we found that miR-9-5p was significantly downregulated in pancreatic cancer tissues and cell lines. The expression levels of miR-9-5p were negatively correlated with tumor stage and vessel invasion. Log-rank t...

journal_title：Biochemical and biophysical research communications

authors： Wang J,Wang B,Ren H,Chen W

更新日期：2019-01-29 00:00:00

abstract：:Astrocytes, a large population of glial cells, detect neurotransmitters and respond by increasing intracellular Ca2+ concentration ([Ca2+]i) and releasing chemical molecules called gliotransmitters. Recently discovered Ca2+ influx through transient receptor potential ankyrin 1 (TRPA1) channels is reported to cause spo...

journal_title：Biochemical and biophysical research communications

authors： Takizawa M,Harada K,Nakamura K,Tsuboi T

更新日期：2018-07-02 00:00:00

abstract：:We isolated mouse CPP32/apopain cDNA, a mammalian homologue most closely related to Ced-3 in C. elegans, and examined the involvement of CPP32 in the apoptosis of nervous system during development. CPP32 is specifically expressed in the trigeminal (V) ganglia, facio-acoustic (VII-VIII) ganglion complex, and dorsal roo...

journal_title：Biochemical and biophysical research communications

authors： Mukasa T,Urase K,Momoi MY,Kimura I,Momoi T

更新日期：1997-02-24 00:00:00

abstract：:The chloroethylnitrosoureas are useful antitumor agents which evidently exert a significant part of their cytotoxic action through the formation of a unique 1-(3-deoxycytidyl), 2-(1-deoxyguanosinyl)ethane cross-link in DNA. It has been suggested that this cross-link is formed from O6-(2-chloro-ethyl)guanine, an initia...

journal_title：Biochemical and biophysical research communications

authors： Parker S,Kirk MC,Ludlum DB

更新日期：1987-11-13 00:00:00