His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase.

Abstract:

:MitoNEET is the first identified iron sulfur protein that located in the mitochondrial outer membrane. We showed that knockdown of mitoNEET did not affect the iron sulfur protein expression in mitochondria and cytoplasm, but significantly reduced the cytosolic aconitase activity. The reduction of aconitase activity was rescued by transfection of wild type mitoNEET, but not by mitoNEET mutants H87C and H87S. Our results confirm the observation that mitoNEET is important in transferring the iron sulfur clusters to the cytosolic aconitase in living cells and the His-87 ligand in mitoNEET plays important role in this process.

authors

Tan G,Liu D,Pan F,Zhao J,Li T,Ma Y,Shen B,Lyu J

doi

10.1016/j.bbrc.2016.01.040

subject

Has Abstract

pub_date

2016-01-29 00:00:00

pages

226-232

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(16)30040-7

journal_volume

470

pub_type

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