Abstract:
:MitoNEET is the first identified iron sulfur protein that located in the mitochondrial outer membrane. We showed that knockdown of mitoNEET did not affect the iron sulfur protein expression in mitochondria and cytoplasm, but significantly reduced the cytosolic aconitase activity. The reduction of aconitase activity was rescued by transfection of wild type mitoNEET, but not by mitoNEET mutants H87C and H87S. Our results confirm the observation that mitoNEET is important in transferring the iron sulfur clusters to the cytosolic aconitase in living cells and the His-87 ligand in mitoNEET plays important role in this process.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Tan G,Liu D,Pan F,Zhao J,Li T,Ma Y,Shen B,Lyu Jdoi
10.1016/j.bbrc.2016.01.040subject
Has Abstractpub_date
2016-01-29 00:00:00pages
226-232issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(16)30040-7journal_volume
470pub_type
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