当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > A lentiviral vector with novel multiple cloning sites: stable transgene expression in vitro and in vivo.

A lentiviral vector with novel multiple cloning sites: stable transgene expression in vitro and in vivo.

Abstract：

:Gene delivery has become an important tool for biological research and gene therapy trials. Lentiviral vector (LV) mediated gene transfer is a preferred approach for stable, sustained transgenic expression. We report here step-wise development of an Indian human immunodeficiency virus type 2 (HIV-2) isolate derived third generation lentiviral vector with a novel, versatile multiple cloning site (MCS) that can also facilitate single step sub-cloning of a PCR amplified transgene cassette by T/A cloning strategy apart from useful cohesive/blunt end cloning. Efficiency of the vector systems was functionally demonstrated by development of a transgenic enhanced green fluorescence protein (GFP) expressing cell line. Further, a GFP down regulated cell line was derived from the said cell line through LV mediated shRNA expression by cloning the GFP-shRNA cassette using the T/A cloning strategy. Subsequently long term expression of GFP transgene in nude mouse spleen/liver was also documented till 30 days. This LV platform with the enhanced user friendly cloning options will be an important advancement in gene transfer technology.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Santhosh CV,Tamhane MC,Kamat RH,Patel VV,Mukhopadhyaya R

doi

10.1016/j.bbrc.2008.04.106

subject

Has Abstract

pub_date

2008-07-04 00:00:00

pages

546-50

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(08)00802-4

journal_volume

371

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • CD47/SIRPα blocking enhances CD19/CD3-bispecific T cell engager antibody-mediated lysis of B cell malignancies.

    abstract：:T cell immunotherapies are promising options in leukemia, among which the CD19/CD3-bispecific T cell engager antibody blinatumomab (MT103) has shown high response rates at very low doses in patients with lymphoma. However, the high CD47 expression in human lymphoma cells has limited the curative effects of blinatumoma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.12.175

    authors： Xu L,Wang S,Li J,Li B

    更新日期：2019-02-12 00:00:00

  • Role of cholesterol in ligand binding and G-protein coupling of serotonin1A receptors solubilized from bovine hippocampus.

    abstract：:The serotonin(1A) (5-HT(1A)) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors. We report here that solubilization of the hippocampal 5-HT(1A) receptor by the zwitterionic detergent CHAPS is accompanied by loss of membrane cholesterol which results in a reduct...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.102

    authors： Chattopadhyay A,Jafurulla M,Kalipatnapu S,Pucadyil TJ,Harikumar KG

    更新日期：2005-02-25 00:00:00

  • HIV-1 Vpr suppresses the cytomegalovirus promoter in a CRL4(DCAF1) E3 ligase independent manner.

    abstract：:Although the Vpr protein of human immunodeficiency virus type 1 (HIV-1) has been shown to act as a transcriptional activator of the HIV-1 LTR and certain host genes, the current study demonstrates that it can also function as a potent inhibitor of the cytomegalovirus (CMV) promoter. Previous studies have shown that th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.02.060

    authors： Liu X,Guo H,Wang H,Markham R,Wei W,Yu XF

    更新日期：2015-04-03 00:00:00

  • Presence of several cytokine-like molecules in molluscan hemocytes.

    abstract：:The presence of a variety of cytokine-like molecules (IL-1 alpha, IL-1 beta, IL-2, IL-6, TNF-alpha) in the hemocytes of two molluscs, i.e., Planorbarius corneus and Viviparus ater, is reported. These molecules are present only in hemocytes with phagocytic activity. These data, together with recent reports on the prese...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2141

    authors： Ottaviani E,Franchini A,Franceschi C

    更新日期：1993-09-15 00:00:00

  • Troglitazone increases expression of E-cadherin and claudin 4 in human pancreatic cancer cells.

    abstract：:We examined the effects of troglitazone on expression of E-cadherin and claudin 4 in human pancreatic cancer cells. Troglitazone dose-dependently increased expression of E-cadherin and claudin 4 mRNA and protein in PK-1 cells. Snail, Slug and ZEB1, mRNAs were not changed by troglitazone, indicating that these three tr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.01.134

    authors： Kumei S,Motomura W,Yoshizaki T,Takakusaki K,Okumura T

    更新日期：2009-03-13 00:00:00

  • Resveratrol attenuates skeletal muscle atrophy induced by chronic kidney disease via MuRF1 signaling pathway.

    abstract：:Skeletal muscle atrophy is an important clinical characteristic of chronic kidney disease (CKD); however, at present, the therapeutic approaches to muscle atrophy induced by CKD are still at an early stage of development. Resveratrol is used to attenuate muscle atrophy in other experimental models, but the effects on ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.04.022

    authors： Sun LJ,Sun YN,Chen SJ,Liu S,Jiang GR

    更新日期：2017-05-20 00:00:00

  • Mannoproteins from Saccharomyces cerevisiae stimulate angiogenesis by promoting the akt-eNOS signaling pathway in endothelial cells.

    abstract：:Mannoproteins (MPs) are a major component of yeast cell walls and consist of high levels of mannose in covalent complexes with proteins. MPs complexly enhance the immune system. We previously isolated a mutant yeast, K48L3, with a higher yield of MP from its cell wall than wild-type Saccharomyces cerevisiae, YPH499. W...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.09.069

    authors： Yoon BH,Lee SM,Chang HI,Ha CH

    更新日期：2019-11-19 00:00:00

  • Preliminary 1H-NMR studies of the interaction between cytochrome c3 and ferredoxin I from Desulfovibrio desulfuricans Norway.

    abstract：:The complex formation of two electron transfer proteins, cytochrome c3 and ferredoxin I from Desulfovibrio desulfuricans Norway, has been shown by 1H-NMR spectroscopy. Presence of ferredoxin I produces ferricytochrome c3 1H-NMR spectrum modifications. The chemical shift of perturbated heme methyl resonances has been u...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91729-2

    authors： Guerlesquin F,Noailly M,Bruschi M

    更新日期：1985-08-15 00:00:00

  • The enantioselective participation of (S)- and (R)-diaminovaleric acids in the formation of delta-aminolevulinic acid in cyanobacteria.

    abstract：:Although it is recognized that 4,5-diaminovaleric acid, formed from glutamate 1-semialdehyde, functions as the intermediate in the last step of delta-aminolevulinic acid formation from glutamate, the enantioselectivity of the participating glutamate 1-semialdehyde aminotransferase for 4,5-diaminovaleric acid has remai...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80955-6

    authors： Friedmann HC,Duban ME,Valasinas A,Frydman B

    更新日期：1992-05-29 00:00:00

  • Genetic manipulation of cardiac Na+/Ca2+ exchange expression.

    abstract：:The Na+/Ca2+ exchanger (NCX) is the primary Ca2+ extrusion mechanism in cardiomyocytes. To further investigate the role of NCX in excitation-contraction coupling and Ca2+ homeostasis, we created murine models with altered expression levels of NCX. Homozygous overexpression of NCX resulted in mild cardiac hypertrophy. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2004.08.038

    authors： Pott C,Goldhaber JI,Philipson KD

    更新日期：2004-10-01 00:00:00

  • Upstream stimulatory factors regulate the C/EBP alpha gene during differentiation of 3T3-L1 preadipocytes.

    abstract：:During adipocyte differentiation, CCAAT/enhancer-binding protein alpha (C/EBPalpha) functions as a pleiotropic transcriptional activator of numerous adipocyte genes. The promoter of the C/EBPalpha gene has an E-box upstream of C/EBP binding site. Deletion or mutation of the E-box decreases promoter activity, suggestin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.01.008

    authors： Kim JW,Monila H,Pandey A,Lane MD

    更新日期：2007-03-09 00:00:00

  • Punicalagin exerts protective effect against high glucose-induced cellular stress and neural tube defects.

    abstract：:Maternal diabetes-induced birth defects remain a significant health problem. Studying the effect of natural compounds with antioxidant properties and minimal toxicities on diabetic embryopathy may lead to the development of new and safe dietary supplements. Punicalagin is a primary polyphenol found in pomegranate juic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.10.024

    authors： Zhong J,Reece EA,Yang P

    更新日期：2015-11-13 00:00:00

  • HASH-1 and E2-2 are expressed in human neuroblastoma cells and form a functional complex.

    abstract：:The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homolog-1 (MASH-1 in mouse and HASH-1 in human) is essential for proper development of olfactory and most peripheral autonomic neurons, and for the formation of distinct neuronal circuits within the central nervous system. We have previousl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3090

    authors： Persson P,Jögi A,Grynfeld A,Påhlman S,Axelson H

    更新日期：2000-07-21 00:00:00

  • Active sites of two orthologous cytochromes P450 2E1: differences revealed by spectroscopic methods.

    abstract：:Cytochromes P450 2E1 of human and minipig origin were examined by absorption spectroscopy under high hydrostatic pressure and by resonance Raman spectroscopy. Human enzyme tends to denature to the P420 form more easily than the minipig form; moreover, the apparent compressibility of the heme active site (as judged fro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.063

    authors： Anzenbacherova E,Hudecek J,Murgida D,Hildebrandt P,Marchal S,Lange R,Anzenbacher P

    更新日期：2005-12-09 00:00:00

  • Modulation of an RGDS binding site on the platelet membrane glycoprotein IIb-IIIa complex.

    abstract：:Fibronectin, von Willebrand factor, and fibrinogen each bind to the glycoprotein IIb-IIIa complex on activated platelets via an arg-gly-asp-ser (RGDS) sequence present within the adhesive proteins. Both the IIb and IIIa polypeptides of the IIb-IIIa complex on thrombin activated platelets are specifically and extensive...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91639-2

    authors： Santoro SA,Lawing WJ Jr

    更新日期：1989-04-14 00:00:00

  • Neurotensin-mediated activation of MAPK pathways and AP-1 binding in the human pancreatic cancer cell line, MIA PaCa-2.

    abstract：:Neurotensin (NT), a gastrointestinal (GI) hormone, binds its receptor (NTR) to stimulate proliferation of normal and neoplastic GI tissues; the molecular mechanisms remain largely undefined. Mitogen-activated protein kinases (MAPKs) are a family of intracellular kinases that transmit mitogenic signals by translocating...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2335

    authors： Ehlers RA,Zhang Y,Hellmich MR,Evers BM

    更新日期：2000-03-24 00:00:00

  • Tropylium tetrafluoroborate, a novel substrate for aldehyde oxidase.

    abstract：:The stabilized carbonium ion salt, tropylium tetrafluoroborate, was oxidized to tropone (cycloheptatrienone) by rabbit liver aldehyde oxidase but not by the closely related molybdenum hydroxylase, xanthine oxidase. The tropylium cation is an aromatic hydrocarbon which lacks the aldehyde, imine, or iminium functional g...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90775-8

    authors： Banks RB,Barnett SD

    更新日期：1986-10-30 00:00:00

  • Elicitors triggering the simultaneous gene expression of antibacterial proteins of the silkworm, Bombyx mori.

    abstract：:Various elicitors were examined by Northern blot analysis to investigate the simultaneous induction of gene expression of antibacterial proteins such as cecropin B, attacin and lebocin from the silkworm, Bombyx mori. Lipopolysaccharide (LPS), lipid A, 2-keto-3-deoxyoctonate (KDO) and peptidoglycan (PG) triggered effic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1429

    authors： Taniai K,Furukawa S,Shono T,Yamakawa M

    更新日期：1996-09-24 00:00:00

  • Galanin increases cytoplasmic calcium in insulin-producing RINm5F cells by activating phospholipase C.

    abstract：:We showed previously that the neuropeptide, galanin, transiently and promptly increases the cytoplasmic concentration of Ca2+, [Ca2+]i, in insulin producing clonal RINm5F cells prior to a reduction in [Ca2+]i. We found that galanin (100 nM) transiently increased the [Ca2+]i in the presence of 15 mM glyceraldehyde and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0550

    authors： Ahrén B

    更新日期：1996-04-05 00:00:00

  • Chronic administration of ezetimibe increases active glucagon-like peptide-1 and improves glycemic control and pancreatic beta cell mass in a rat model of type 2 diabetes.

    abstract：:Ezetimibe is a cholesterol-lowering agent targeting Niemann-Pick C1-like 1, an intestinal cholesterol transporter. Inhibition of intestinal cholesterol absorption with ezetimibe may ameliorate several metabolic disorders including hepatic steatosis and insulin resistance. In this study, we investigated whether chronic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.02.129

    authors： Yang SJ,Choi JM,Kim L,Kim BJ,Sohn JH,Kim WJ,Park SE,Rhee EJ,Lee WY,Oh KW,Park SW,Kim SW,Park CY

    更新日期：2011-04-01 00:00:00

  • Roles of antibodies to influenza A virus hemagglutinin, neuraminidase, and M2e in conferring cross protection.

    abstract：:Although neuraminidase (NA) is the second major viral glycoprotein of influenza virus, its immune mechanism as a vaccine target has been less considered. Here we compared the properties of antibodies and the efficacy of cross protection by N1 and N2 NA proteins, inactivated split influenza vaccines (split), and tandem...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.09.011

    authors： Kim YJ,Ko EJ,Kim MC,Lee YN,Kim KH,Jung YJ,Kang SM

    更新日期：2017-11-04 00:00:00

  • In vitro transformation of mesenchymal stem cells by oncogenic H-rasVal12.

    abstract：:Tissue stem cells may serve as progenitors for malignant tumors derived from the same tissue. Here, we report the establishment of immortalized human mesenchymal stem cells (ihMSC) and tested the feasibility of using ihMSC as presarcomatous cells. Immortalization was achieved by introducing the genes for human telomer...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.137

    authors： Shima Y,Okamoto T,Aoyama T,Yasura K,Ishibe T,Nishijo K,Shibata KR,Kohno Y,Fukiage K,Otsuka S,Uejima D,Nakayama T,Nakamura T,Kiyono T,Toguchida J

    更新日期：2007-02-02 00:00:00

  • Identification of the hRDH-E2 gene, a novel member of the SDR family, and its increased expression in psoriatic lesion.

    abstract：:To identify novel psoriasis-associated genes, we focused on several ESTs (expressed sequence tags) whose expression was predominantly increased in the affected skin in patients with psoriasis vulgaris, as assessed by microarray assay. In this paper, a full-length cDNA corresponding to one of those ESTs (AI440266) was ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02344-6

    authors： Matsuzaka Y,Okamoto K,Tsuji H,Mabuchi T,Ozawa A,Tamiya G,Inoko H

    更新日期：2002-10-11 00:00:00

  • Purification of a novel eIF-2 alpha protein kinase from calf brain.

    abstract：:A new eukaryotic initiation factor 2 kinase has been purified for the first time from calf brain cytosol. The purification of a nonabundant novel protein kinase activity, designated as PKI, that phosphorylates the alpha subunit of eukaryotic initiation factor 2 is described. The protein kinase activity was assayed usi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90998-3

    authors： Alcázar A,Méndez E,Martín ME,Salinas M

    更新日期：1990-02-14 00:00:00

  • Inhibition by adiponectin of IL-8 production by human macrophages upon coculturing with late apoptotic cells.

    abstract：:Adiponectin, an adipocyte-derived hormone, reportedly suppresses the production of TNF-alpha and IL-6 by LPS-stimulated human or porcine macrophages, and the phagocytosis of microbeads by human macrophages. In this study, we used a high molecular weight form of adiponectin purified from human plasma to examine its eff...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.07.016

    authors： Saijo S,Nagata K,Nakano Y,Tobe T,Kobayashi Y

    更新日期：2005-09-09 00:00:00

  • Activation of bovine neutrophil oxidase in a cell free system. GTP-dependent formation of a complex between a cytosolic factor and a membrane protein.

    abstract：:The effect of guanine nucleotides on activation of the O2-. generating oxidase in a cell free system consisting of bovine neutrophils membranes, cytosol and arachidonic acid has been studied. In a complete system, GTP-gamma-S was stimulatory and GDP-beta-S inhibitory. When cytosol was omitted, both nucleotides acted a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80382-6

    authors： Doussiere J,Pilloud MC,Vignais PV

    更新日期：1988-05-16 00:00:00

  • Direct interaction of menin leads to ubiquitin-proteasomal degradation of β-catenin.

    abstract：:Menin, encoded by the multiple endocrine neoplasia type 1 (MEN1) gene, is a tumor suppressor and transcription regulator. Menin interacts with various proteins as a scaffold protein and is proposed to play important roles in multiple physiological and pathological processes by controlling gene expression, proliferatio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.08.011

    authors： Kim B,Song TY,Jung KY,Kim SG,Cho EJ

    更新日期：2017-10-07 00:00:00

  • Mitochondria and microsomal membranes have a free radical reductase activity that prevents chromanoxyl radical accumulation.

    abstract：:Enzyme-dependent mechanisms which prevent accumulation of chromanoxyl radicals derived from the vitamin E analogue, 2,2,5,7,8-pentamethyl-6-hydroxycromane (PMC), were characterized in rat liver microsomal and mitochondrial membranes. The free radical oxidation product of PMC (chromanoxyl radical) was generated in memb...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92427-3

    authors： Packer L,Maguire JJ,Mehlhorn RJ,Serbinova E,Kagan VE

    更新日期：1989-02-28 00:00:00

  • Rapamycin alleviates cisplatin-induced ototoxicity in vivo.

    abstract：BACKGROUND:Cisplatin-induced ototoxicity affects a high percentage of new cancer patients worldwide. The detailed mechanism of cisplatin-induced ototoxicity is not completely understood. We investigated whether rapamycin could protect rats from cisplatin-induced ototoxicity. METHODS:Forty-eight male Wistar rats were r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.04.123

    authors： Fang B,Xiao H

    更新日期：2014-06-13 00:00:00

  • ZNF322, a novel human C2H2 Kruppel-like zinc-finger protein, regulates transcriptional activation in MAPK signaling pathways.

    abstract：:Cardiac differentiation involves a cascade of coordinated gene expression that regulates cell proliferation and matrix protein formation in a defined temporal-spatial manner. The C(2)H(2) zinc finger-containing transcription factors have been implicated as critical regulators of multiple cardiac-expressed genes and ar...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.10.183

    authors： Li Y,Wang Y,Zhang C,Yuan W,Wang J,Zhu C,Chen L,Huang W,Zeng W,Wu X,Liu M

    更新日期：2004-12-24 00:00:00