当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Purification of a novel eIF-2 alpha protein kinase from calf brain.

Purification of a novel eIF-2 alpha protein kinase from calf brain.

Abstract：

:A new eukaryotic initiation factor 2 kinase has been purified for the first time from calf brain cytosol. The purification of a nonabundant novel protein kinase activity, designated as PKI, that phosphorylates the alpha subunit of eukaryotic initiation factor 2 is described. The protein kinase activity was assayed using purified initiation factor 2 as a substrate and was purified by ammonium sulphate precipitation, conventional chromatography in heparin-Sepharose and phosphocellulose and by high performance size exclusion and anion exchange chromatographies. The protein kinase activity elutes in the region of 140,000 in the size exclusion chromatography and is associated with two different polypeptides a and b, with relative molecular masses of 38,000 and 20,000 and an approximate ratio of 2.5-3.0:1. The protein kinase does not phosphorylate casein or histones and it is independent of cyclic nucleotides. It can be classified as a serine kinase since the phosphorylation of the alpha subunit of eIF-2 is produced in serine residues. Under these conditions none of the kinase subunits are phosphorylated.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Alcázar A,Méndez E,Martín ME,Salinas M

doi

10.1016/0006-291x(90)90998-3

subject

Has Abstract

pub_date

1990-02-14 00:00:00

pages

1237-44

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(90)90998-3

journal_volume

166

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Nucleotides maintain the activity of Cav1.2 channels in guinea-pig ventricular myocytes.

    abstract：:The activity of Cav1.2 Ca(2+) channels is maintained in the presence of calmodulin and ATP, even in cell-free patches, and thus a channel ATP-binding site has been suggested. In this study, we examined whether other nucleotides, such as GTP, UTP, CTP, ADP and AMP, could be substituted for ATP in guinea-pig ventricular...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.03.111

    authors： Liu SY,Xu JJ,Minobe E,Gao QH,Feng R,Zhao MM,Guo F,Yang L,Hao LY,Kameyama M

    更新日期：2015-05-08 00:00:00

  • Cyclic AMP-specific phosphodiesterase 4 inhibitors promote ABCA1 expression and cholesterol efflux.

    abstract：:ATP cassette binding protein 1 (ABCA1) controls the apolipoprotein-mediated cholesterol efflux pathway and determines plasma HDL levels. Although cAMP is known to promote ABCA1 expression and cholesterol efflux from cells, it has not been determined whether cyclic nucleotide phosphodiesterase (PDE) isoforms regulate t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6259

    authors： Lin G,Bornfeldt KE

    更新日期：2002-01-18 00:00:00

  • The role of ELK3 to regulate peritumoral lymphangiogenesis and VEGF-C production in triple negative breast cancer cells.

    abstract：:Tumor-induced lymphangiogenesis, a major conduit for cancer cell dissemination from the primary tumor site to lymph nodes and beyond, eventually leads to metastasis in cancer patients. Given the recent evidence revealing that the suppression of ELK3 inhibits the metastasis of triple-negative breast cancer cells, we ai...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.02.030

    authors： Oh N,Park JI,Park JH,Kim KS,Lee DR,Park KS

    更新日期：2017-03-18 00:00:00

  • Cyclic AMP decreases LDL catabolism and cholesterol synthesis in the human hepatoma cell line HepG2.

    abstract：:A 24h pretreatment of the human hepatoma cell line HepG2 with dibutyryl cyclic AMP in the presence of theophylline induced a dose dependent decrease in low density lipoprotein binding, uptake and degradation. This effect is most likely due to a reduction of the LDL receptor number. Sterol synthesis from sodium acetate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80858-1

    authors： Maziere C,Maziere JC,Salmon S,Auclair M,Mora L,Moreau M,Polonovski J

    更新日期：1988-10-14 00:00:00

  • In situ hybridization patterns of zebrafish homeobox genes homologous to Hox-2.1 and En-2 of mouse.

    abstract：:Vertebrate homeobox genes have been identified on the basis of sequence homology to Drosophila segmentation and homeotic genes. We have analyzed the spatial distribution patterns in hatching larvae of transcripts from two different types of zebrafish (Brachydanio rerio) homeobox genes. Tritiated DNA fragments from one...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80266-3

    authors： Njølstad PR,Fjose A

    更新日期：1988-12-15 00:00:00

  • A novel protease inhibitor causes inclusion vacuole reduction and disrupts the intracellular growth of Chlamydia trachomatis.

    abstract：:Chlamydia (C.) trachomatis, characterized by a unique biphasic life cycle, is an obligate intracellular bacterial pathogen which is responsible for the highest number of sexually transmitted bacterial infections globally. However, its pathogenic mechanisms have not been fully elucidated because of its unique developme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.05.184

    authors： Zhou Y,Lu X,Huang D,Lu Y,Zhang H,Zhang L,Yu P,Wang F,Wang Y

    更新日期：2019-08-13 00:00:00

  • Long non-coding RNA-422 acts as a tumor suppressor in colorectal cancer.

    abstract：:Colorectal cancer (CRC) is one of the most frequent cancer in numerous of countries worldwidely. The initiation and progression of CRC is an extremely complex process, and have been suggested a correlation with Long non-coding RNAs (lncRNAs). Our results showed that lncRNA-422(ENST00000415820) significantly downregula...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.10.076

    authors： Shao Q,Xu J,Deng R,Wei W,Zhou B,Yue C,Zhu M,Huang X,Zhu H

    更新日期：2018-01-01 00:00:00

  • Eotaxin is a potent chemotaxin for human basophils.

    abstract：:We studied the effect of eotaxin, a novel eosinophil-active CC chemokine with high target cell specificity, on human basophils. Eotaxin induced higher levels of chemotactic response with a lower ED50 compared with RANTES in basophils; half-maximal migration occurred at a concentration of approximately 3 nM. On the oth...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6100

    authors： Yamada H,Hirai K,Miyamasu M,Iikura M,Misaki Y,Shoji S,Takaishi T,Kasahara T,Morita Y,Ito K

    更新日期：1997-02-13 00:00:00

  • Stress-induced p53 runs a transcription-independent death program.

    abstract：:The transcription-independent p53-mediated apoptotic response has obtained a solid mechanistic basis in recent years. A fraction of stress-induced wild type p53 protein rapidly translocates to mitochondria in response to genotoxic, hypoxic, and oxidative stresses in established cell lines and primary cells, as well as...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2005.03.187

    authors： Erster S,Moll UM

    更新日期：2005-06-10 00:00:00

  • Synergistic potentiation of thiazolidinedione-induced ST 13 preadipocyte differentiation by RAR synergists.

    abstract：:Peroxisome proliferator-activated receptor gamma (PPAR gamma) belongs to a nuclear receptor super family that functions as a master regulator of adipocyte differentiation. PPAR gamma binds its DNA response element together with a partner, retinoid X receptor (RXR), in fat cells. Five RXR ligands (HX600, HX630, DA022, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4172

    authors： Sato M,Yajima Y,Kawashima S,Tanaka K,Kagechika H

    更新日期：2001-01-26 00:00:00

  • Drosophila melanogaster larval hemolymph protein mapping.

    abstract：:With the completion of the genome sequence of Drosophila melanogaster the importance of constructing a proteome map is to be considered. Therefore, with the application of recent advances in proteomic analysis approaches, a protein map of D. melanogaster larvae hemolymph proteins was obtained using 2-DE in the range o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.10.156

    authors： Guedes Sde M,Vitorino R,Tomer K,Domingues MR,Correia AJ,Amado F,Domingues P

    更新日期：2003-12-19 00:00:00

  • Membrane topology of human AGPAT3 (LPAAT3).

    abstract：:Integral membrane lysophospholipid acyltransferases (AT) are involved in many reactions that produce phospholipids and triglycerides. Enzymes that utilize lysophosphatidic acid (LPA) as an acceptor substrate have been termed LPAATs, and several are members of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) ge...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.05.149

    authors： Schmidt JA,Yvone GM,Brown WJ

    更新日期：2010-07-09 00:00:00

  • Selective destabilization of soluble amyloid beta oligomers by divalent metal ions.

    abstract：:Aggregation of the amyloid beta (Abeta) peptide yields both fibrillar precipitates and soluble oligomers, and is associated with Alzheimer's disease (AD). In vitro, Cu(2+) and Zn(2+) strongly bind Abeta and promote its precipitation. However, less is known about their interactions with the soluble oligomers, which are...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.04.056

    authors： Garai K,Sengupta P,Sahoo B,Maiti S

    更新日期：2006-06-23 00:00:00

  • ATP antagonizes the crowding-induced destabilization of the human eye-lens protein γS-crystallin.

    abstract：:In lens, αβγ-crystallins accounting for ∼90% of ocular proteins with concentrations >400 mg/ml need to remain soluble for the whole life-span and their aggregation can lead to cataract. Mysteriously, despite being a metabolically-quiescent organ, lens maintains ATP concentrations of 3-7 mM. Very recently, ATP was prop...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.04.014

    authors： He Y,Kang J,Song J

    更新日期：2020-06-11 00:00:00

  • Alcohol consumption during adolescence alters the hippocampal response to traumatic brain injury.

    abstract：:Binge drinking is the consumption of large volumes of alcohol in short periods and exerts its effects on the central nervous system, including the hippocampus. We have previously shown that binge drinking alters mitochondrial dynamics and induces neuroinflammation in the hippocampus of adolescent rats. Mild traumatic ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.05.160

    authors： Mira RG,Lira M,Quintanilla RA,Cerpa W

    更新日期：2020-07-30 00:00:00

  • Angiogenin is involved in morphological changes and angiogenesis in the ovary.

    abstract：:Angiogenin is a potent angiogenic factor secreted by cultured tumor cells and is found in various normal organs and tissues. The ovary is one of the adult organs in which angiogenesis normally occurs during the female reproductive cycle. In this study, we examined whether angiogenin protein is localized and if angioge...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0359

    authors： Lee HS,Lee IS,Kang TC,Jeong GB,Chang SI

    更新日期：1999-04-02 00:00:00

  • Molecular cloning and 3D model of first cytochrome P450 from CYP3A subfamily in saltwater crocodile (Crocodylus porosus).

    abstract：:Cytochrome P450s (CYPs) play critical role in oxidative metabolism of numerous xenobiotics and endogenous compounds. The first CYP3A subfamily member in saltwater crocodile has been cloned and modelled for three-dimensional (3D) structure. The full-length cDNA was obtained employing reverse transcription polymerase ch...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.10.079

    authors： Tabassum R

    更新日期：2019-08-27 00:00:00

  • Toosendanin induces caspase-dependent apoptosis through the p38 MAPK pathway in human gastric cancer cells.

    abstract：:Although many advances have been made in the treatment of gastric cancer (GC), numerous difficulties, such as the emergence of chemo-drug resistance, continue to lead to disappointing GC prognoses. Thus, novel alternative strategies are urgently needed. The use of natural products could be a viable option to treat GC....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.093

    authors： Zhou Q,Wu X,Wen C,Wang H,Wang H,Liu H,Peng J

    更新日期：2018-10-20 00:00:00

  • Expression of the C-terminal domain of novel human SR-A1 protein: interaction with the CTD domain of RNA polymerase II.

    abstract：:We have recently cloned a new member of the human Ser/Arg-rich superfamily (SR) of pre-mRNA splicing factors, SR-A1. Members of the SR family of proteins have been shown to interact with the C-terminal domain (CTD) of the large subunit of RNA polymerase II, and participate in pre-mRNA splicing. The largest subunit of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.06.053

    authors： Katsarou ME,Papakyriakou A,Katsaros N,Scorilas A

    更新日期：2005-08-19 00:00:00

  • The biogenesis of SARS-CoV-2 spike glycoprotein: multiple targets for host-directed antiviral therapy.

    abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19 (coronavirus disease-19), represents a far more serious threat to public health than SARS and MERS coronaviruses, due to its ability to spread more efficiently than its predecessors. Currently, there is no worldwide-approved ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.10.080

    authors： Santopolo S,Riccio A,Santoro MG

    更新日期：2020-11-28 00:00:00

  • Differential regulation of multiple neuroreceptors in a somatic cell hybrid by inhibitors of glycoprotein processing.

    abstract：:Specific binding of [3H][D-Ala2,D-Leu5]enkephalin, [3H]ethylketocyclazocine, 5-[3H]hydroxytryptamine, and [3H]spiperone was examined in neuroblastoma-brain hybrid cell line NCB-20 following exposure to inhibitors of N-linked protein glycosylation (tunicamycin, TM) and oligosaccharide processing (swainsonine, SW). TM t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90403-1

    authors： McLawhon RW,Berry-Kravis E,Dawson G

    更新日期：1986-02-13 00:00:00

  • Tri-iodothyronine preconditioning protects against liver ischemia reperfusion injury through the regulation of autophagy by the MEK/ERK/mTORC1 axis.

    abstract：BACKGROUND AND OBJECTIVES:The autophagy pathway has previously been suggested as an important protective factor in liver injury. The purpose of this study is to demonstrate the protective, autophagy-modulating effect of tri-iodothyronine (T3) on liver ischemia reperfusion injury. METHODS:Liver ischemia reperfusion was...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.10.080

    authors： Yang J,Wang Y,Sui M,Liu F,Fu Z,Wang QX

    更新日期：2015-11-27 00:00:00

  • Purification and characterization of a new form of cytochrome P-450 (LM2b) from untreated male rabbit liver microsomes.

    abstract：:A new form of cytochrome P-450 has been purified from untreated male rabbit liver microsomes. This form was designated P-450 LM2b on the basis of its electrophoretic mobility on SDS polyacrylamide gel, where it migrates as a polypeptide of apparent molecular weight of 50,250. This hemoprotein exhibits a maximum at 448...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90364-5

    authors： Bonfils C,Larroque C,Maurel P

    更新日期：1986-02-13 00:00:00

  • Inactivation of lipoprotein lipase in 3T3-L1 adipocytes by angiopoietin-like protein 4 requires that both proteins have reached the cell surface.

    abstract：:Lipoprotein lipase (LPL) and angiopoietin-like protein 4 (Angptl4) were studied in 3T3-L1 adipocytes. Transfections of the adipocytes with Angptl4 esiRNA caused reduction of the expression of Angptl4 to about one fourth of that in cells treated with vehicle only. This resulted in higher levels of LPL activity both on ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.11.013

    authors： Makoveichuk E,Vorrsjö E,Olivecrona T,Olivecrona G

    更新日期：2013-11-29 00:00:00

  • Metallothionein rescues hypoxia-inducible factor-1 transcriptional activity in cardiomyocytes under diabetic conditions.

    abstract：:Metallothionein (MT) is effective in the prevention of diabetic cardiomyopathy, and hypoxia-inducible factor-1 (HIF-1) is known to control vascular endothelial growth factor (VEGF) gene expression and regulate angiogenesis in diabetic hearts. We examined whether or not MT affects HIF-1 activity in the heart of diabeti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.06.057

    authors： Feng W,Wang Y,Cai L,Kang YJ

    更新日期：2007-08-17 00:00:00

  • Hepatocyte growth factor increases mitochondrial mass in glioblastoma cells.

    abstract：:Hepatocyte growth factor/scatter factor (HGF) is a multifunctional growth factor that is linked to the initiation and/or progression of numerous malignancies. HGF also alters cancer cell responses to DNA damaging cytotoxic agents. Many cell responses to Met activation require alterations in metabolic activity but how ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.05.064

    authors： Xia S,Laterra J

    更新日期：2006-07-14 00:00:00

  • Enhancement of phage-mediated gene transfer by nuclear localization signal.

    abstract：:The cell membrane and the nuclear membrane are two major barriers hindering the free movement of various macromolecules through animal cells. Nevertheless, some proteins can actively bypass these barriers by dint of intrinsic peptidic signals, so incorporation of these signals might improve the efficacy of artificial ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02282-9

    authors： Akuta T,Eguchi A,Okuyama H,Senda T,Inokuchi H,Suzuki Y,Nagoshi E,Mizuguchi H,Hayakawa T,Takeda K,Hasegawa M,Nakanishi M

    更新日期：2002-10-04 00:00:00

  • Proteinase activity in the white shrimp (Penaeus vannamei) clotting protein.

    abstract：:The clottable protein (CP) was isolated from white shrimp, Penaeus vannamei plasma as a 400-kDa protein that splits to two identical 200-kDa subunits when it is reduced with 2-ME. However, using DTT as reducing agent, four main bands were observed; two of them (179 and 125 kDa) had the same N-terminus sequence of the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5595

    authors： Reyes-Izquierdo T,Vargas-Albores F

    更新日期：2001-09-21 00:00:00

  • Catenated dimers and knotted DNA structures: putative intermediates in the replication of T. cruzi kinetoplast minicircle DNA.

    abstract：:Upon centrifugation of gently lysed T. cruzi cells through a sucrose gradient, a free DNA fraction was shown to contain catenated dimers and knotted DNA structures. Southern hybridization and electron microscopic studies indicated that both of these structures derived from minicircle DNA, the major component of T. cru...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91512-b

    authors： Valenzuela MS,Bardhan S,Krishnamani MR,Siddiqui KA

    更新日期：1991-01-31 00:00:00

  • Circadian variations of prostaglandins D2, E2, and F2 alpha in the cerebrospinal fluid of anesthetized rats.

    abstract：:Circadian variations in the levels of prostaglandins (PGs) D2, E2, and F2 alpha were studied in the cerebrospinal fluid (CSF) of rats. We collected CSF samples from the cisterna magna of anesthetized rats at different clock-hours (1000, 1400, 1800, 2200, 0200, and 0600 hr), and measured the concentrations of the three...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2177

    authors： Pandey HP,Ram A,Matsumura H,Satoh S,Hayaishi O

    更新日期：1995-08-15 00:00:00