当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Purification and characterization of a new form of cytochrome P-450 (LM2b) from untreated male rabbit liver microsomes.

Purification and characterization of a new form of cytochrome P-450 (LM2b) from untreated male rabbit liver microsomes.

Abstract：

:A new form of cytochrome P-450 has been purified from untreated male rabbit liver microsomes. This form was designated P-450 LM2b on the basis of its electrophoretic mobility on SDS polyacrylamide gel, where it migrates as a polypeptide of apparent molecular weight of 50,250. This hemoprotein exhibits a maximum at 448.5 nm in the Soret band of the CO-Ferrous state spectrum. On the basis of its molecular, spectral, enzymologic and immunologic data, P-450 LM2b was shown to be distinct from the other P-450 forms, already characterized in rabbit liver microsomes. However P-450 LM2b and P-450 LM3b appear to be immunologically related proteins.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Bonfils C,Larroque C,Maurel P

doi

10.1016/0006-291x(86)90364-5

subject

Has Abstract

pub_date

1986-02-13 00:00:00

pages

1101-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(86)90364-5

journal_volume

134

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Molecular cloning of Ebitein1: a novel extracellular signal-regulated kinase 2-binding protein in testis.

    abstract：:We cloned a cDNA encoding a novel extracellular signal-regulated kinase (ERK) 2-binding protein, EBITEIN1, by yeast two-hybrid screening. Northern and Western blotting experiments showed that the transcript and protein were expressed in the testes. Furthermore, immunohistochemical experiments showed that EBITEIN1 exis...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.01.078

    authors： Miura K,Imaki J

    更新日期：2008-04-04 00:00:00

  • Cadmium binding studies to the earthworm Lumbricus rubellus metallothionein by electrospray mass spectrometry and circular dichroism spectroscopy.

    abstract：:The earthworm Lumbricus rubellus has been found to inhabit cadmium-rich soils and accumulate cadmium within its tissues. Two metallothionein (MT) isoforms (1 and 2) have been identified and cloned from L. rubellus. In this study, we address the metalation status, metal coordination, and structure of recombinant MT-2 f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.023

    authors： Ngu TT,Sturzenbaum SR,Stillman MJ

    更新日期：2006-12-08 00:00:00

  • Degrading activity for human parathyroid hormone [PTH-(1-84)] in rat osteoblast-like osteosarcoma cell line UMR106.

    abstract：:The degrading activity for human parathyroid hormone [hPTH-(1-84)] was studied in a rat osteoblast-like osteosarcoma cell line UMR106. At 37 C,UMR106 cells degraded hPTH-(1-84) into fragments in a time-dependent manner, which was shown by a radioimmunoassay with the use of antibody recognizing the C-terminal and middl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80238-8

    authors： Yamaguchi T,Baba H,Fukase M,Kinoshita Y,Fujimi T,Fujita T

    更新日期：1986-12-15 00:00:00

  • Involvement of lysosomal integral membrane protein-2 in the activation of autophagy.

    abstract：:Lysosomal integral membrane protein-2 (LIMP-2) is a type III transmembrane protein that is highly glycosylated and mainly localized to the lysosomal membrane. The diverse functions of LIMP-2 are currently being uncovered; however, its participation in macroautophagy, usually described as autophagy, has not yet been we...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.09.114

    authors： Sakane H,Urabe J,Nakahira S,Hino K,Miyata N,Akasaki K

    更新日期：2020-12-17 00:00:00

  • Involvement of proton-sensing receptor TDAG8 in the anti-inflammatory actions of dexamethasone in peritoneal macrophages.

    abstract：:Dexamethasone (DEX), a potent glucocorticoid, increased the expression of T-cell death associated gene 8 (TDAG8), a proton-sensing G protein-coupled receptor, which is associated with the enhancement of acidic pH-induced cAMP accumulation, in peritoneal macrophages. We explored the role of increased TDAG8 expression i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.10.122

    authors： He XD,Tobo M,Mogi C,Nakakura T,Komachi M,Murata N,Takano M,Tomura H,Sato K,Okajima F

    更新日期：2011-12-02 00:00:00

  • Characterization of the enzymatic mechanism of gamma-momorcharin, a novel ribosome-inactivating protein with lower molecular weight of 11,500 purified from the seeds of bitter gourd (Momordica charantia).

    abstract：:The enzymatic mechanism of a small ribosome-inactivating protein, gamma-momorcharin, purified from the seeds of Momordica charantia, has been characterized. By SDS-polyacrylamide and electrospray ionization mass spectrometry, its molecular weight was measured to be 11,500 daltons which is much lower than other RIPs kn...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1794

    authors： Pu Z,Lu BY,Liu WY,Jin SW

    更新日期：1996-12-04 00:00:00

  • Microtubule-associated proteins bind to 30 kDa and 60 kDa proteins of rat brain mitochondria: visualization by ligand blotting.

    abstract：:Axonal transport of mitochondria is a microtubule-associated movement. Microtubule-mitochondria interactions were studied in vitro using organelles isolated from rat brain. Thanks to the ligand blotting method we were able to show two mitochondrial membrane proteins with apparent molecular masses of 30 kDa and 60 kDa ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90435-9

    authors： Rendon A,Filliol D,Jancsik V

    更新日期：1987-12-16 00:00:00

  • Heterogeneity of insulin-like growth factor-I affinity for the insulin-like growth factor-II receptor: comparison of natural, synthetic and recombinant DNA-derived insulin-like growth factor-I.

    abstract：:Although insulin-like growth factors (IGF) I and II bind with high affinity to structurally discrete receptors, they bind with a lesser affinity to each other's receptor. We have evaluated the affinity of five different IGF-I preparations (three natural IGF-I preparations, one synthetic preparation, and one recombinan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90650-4

    authors： Rosenfeld RG,Conover CA,Hodges D,Lee PD,Misra P,Hintz RL,Li CH

    更新日期：1987-02-27 00:00:00

  • Surface modification of amorphous nanosilica particles suppresses nanosilica-induced cytotoxicity, ROS generation, and DNA damage in various mammalian cells.

    abstract：:Recently, nanomaterials have been utilized in various fields. In particular, amorphous nanosilica particles are increasingly being used in a range of applications, including cosmetics, food technology, and medical diagnostics. However, there is concern that the unique characteristics of nanomaterials might induce unde...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.09.132

    authors： Yoshida T,Yoshioka Y,Matsuyama K,Nakazato Y,Tochigi S,Hirai T,Kondoh S,Nagano K,Abe Y,Kamada H,Tsunoda S,Nabeshi H,Yoshikawa T,Tsutsumi Y

    更新日期：2012-11-02 00:00:00

  • Neuroprotective effects of memantine via enhancement of autophagy.

    abstract：INTRODUCTION:Chemical intervention of autophagy has been investigated in clinical trials for various age-related conditions such as sarcopenia and neurodegeneration. However, at present, no autophagy inducer has been established as a disease-modifying agent against neurodegenerative diseases. METHODS:We screened a lib...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.08.025

    authors： Hirano K,Fujimaki M,Sasazawa Y,Yamaguchi A,Ishikawa KI,Miyamoto K,Souma S,Furuya N,Imamichi Y,Yamada D,Saya H,Akamatsu W,Saiki S,Hattori N

    更新日期：2019-10-08 00:00:00

  • A methylation-dependent DNA-binding activity recognising the methylated promoter region of the mouse Xist gene.

    abstract：:Differential methylation of CpG sites in the promoter region of the mouse Xist gene is correlated with Xist expression and X-chromosome inactivation in the female. Using oligonucleotides encompassing the differentially methylated sites as probes in band-shift assays, we have identified a nuclear protein which binds to...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6876

    authors： Huntriss J,Lorenzi R,Purewal A,Monk M

    更新日期：1997-06-27 00:00:00

  • Spatial and temporal expression of cellular retinoic acid binding protein (CRABP) along the anteroposterior axis in the central nervous system of mouse embryos.

    abstract：:In the central nervous system of 11.5-day mouse embryos, the expression of CRABP was spatially restricted to the anteroposterior axis. CRABP was most strongly expressed in the rhombencephalon and the anterior part of the neural tube. In 14-day mouse embryo, CRABP drastically decreased in the brain and the anterior par...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91992-2

    authors： Momoi T,Hanaoka K,Momoi M

    更新日期：1990-06-29 00:00:00

  • Curative and protective effects of L-arginine on carbon tetrachloride-induced hepatotoxicity in mice.

    abstract：:L-arginine may aid in the liver detoxification and may benefit in the treatment of liver disorders such as liver injury. The present study was to investigate the possible protective and curative effects of L-arginine on carbon tetrachloride (CCl(4)) induced hepatotoxicity. Mice received a single dose of CCl(4). L-argi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.05.102

    authors： Saad EA

    更新日期：2012-06-22 00:00:00

  • Acyl-trafficking in membrane phospholipid fatty acid turnover: the transfer of fatty acid from the acyl-L-carnitine pool to membrane phospholipids in intact human erythrocytes.

    abstract：:In this work we have investigated the transfer of radioactive palmitic acid between membrane phospholipids and acyl-L-carnitines in intact human erythrocytes. During the incubation period of labeled erythrocyte in non-defatted bovine serum albumin, radioactivity in phosphatidylcholine and phosphatidylethanolamine incr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81500-1

    authors： Arduini A,Tyurin V,Tyuruna Y,Arrigoni-Martelli E,Molajoni F,Dottori S,Federici G

    更新日期：1992-08-31 00:00:00

  • Targeting sphingosine kinase 2 by ABC294640 inhibits human skin squamous cell carcinoma cell growth.

    abstract：:The activity of ABC294640, a small-molecular sphingosine kinase 2 (SphK2) inhibitor, in human skin squamous cell carcinoma (SCC) cells was tested in this study. SphK2 mRNA and protein are expressed in established (A431 cheilocarcinoma cell line) and primary human skin SCC cells. ABC294640 dose-dependently inhibited su...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.02.075

    authors： Zhou J,Chen J,Yu H

    更新日期：2018-03-04 00:00:00

  • Jagged-1 juxtamembrane region: biochemical characterization and cleavage by ADAM17 (TACE) catalytic domain.

    abstract：:Ectodomain shedding of membrane receptors and ligands carried out by ADAMs (A disintegrin and metalloprotease) plays a major role in several signaling pathways, including Notch. The grounds of substrate recognition, however, are poorly understood. We demonstrate that a recombinant protein corresponding to the juxtamem...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.02.022

    authors： Coglievina M,Guarnaccia C,Zlatev V,Pongor S,Pintar A

    更新日期：2013-03-22 00:00:00

  • Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts.

    abstract：:The neuropeptide Y (NPY) system is known as one of the major neural signaling pathways. NPY, produced by peripheral tissues including osteoblasts, is known to bind to the Y1 receptor. Recently, osteoblast-specific Y1 receptor knockout mice were developed and were found to have a high bone mass phenotype, indicating a ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.09.014

    authors： Kurebayashi N,Sato M,Fujisawa T,Fukushima K,Tamura M

    更新日期：2013-10-04 00:00:00

  • Nuclear localization of tumor necrosis factor-alpha in human osteoblast-like cells.

    abstract：:We have studied the internalization and subsequent nuclear localization of [125I]-rTNF-alpha in a human osteoblast-like cell line, HOS TE85. TNF-alpha is rapidly internalized and optimum cellular levels are achieved in approximately 20 minutes at 37 degrees C. A portion of the internalized cytokine is translocated to ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1865

    authors： Panagakos FS,Kumar S

    更新日期：1994-06-30 00:00:00

  • Turpentine-induced decrease of alpha 1-foetoprotein in the serum of the developing rat: a novel parameter of the inflammatory response.

    abstract：:The acute phase of an inflammation induced in young rats, at various ages between 1 and 21 days after birth, by a single subcutaneous turpentine injection, is accompanied by a 35%-45% decrease of the alpha 1-foetoprotein serum concentrations. This fall is demonstrated with equilibrium dialysis binding methods, evidenc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91032-x

    authors： Savu L,Zouaghi H,Nunez EA

    更新日期：1983-02-10 00:00:00

  • The integrated stress response and proteotoxicity in cancer therapy.

    abstract：:A variety of different forms of cellular stress can cause protein misfolding and aggregation and proteotoxicity. The cytoprotective response to proteotoxicity is termed the integrated stress response and involves 4 distinct serine/threonine protein kinases that converge on the translation initiation factor eIF2α, resu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2016.11.047

    authors： McConkey DJ

    更新日期：2017-01-15 00:00:00

  • Delivery of anti-GAP-43 antibodies into neuroblastoma cells reduces growth cone size.

    abstract：:We have previously demonstrated that antibodies to the growth-associated protein, GAP-43, introduced intracellularly using a lipid carrier inhibited neurite outgrowth in NB2a/d1 neuroblastoma cells, and that culturing of these cells on adhesive substrates such as laminin or poly-L-lysine overcame this restriction. The...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2204

    authors： Shea TB

    更新日期：1994-08-30 00:00:00

  • The NADase CD38 is induced by factors secreted from senescent cells providing a potential link between senescence and age-related cellular NAD+ decline.

    abstract：:Tissue nicotinamide adenine dinucleotide (NAD+) decline has been implicated in aging. We have recently identified CD38 as a central regulator involved in tissue NAD+ decline during the aging process. CD38 is an ecto-enzyme highly expressed in endothelial and inflammatory cells. To date, the mechanisms that regulate CD...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.199

    authors： Chini C,Hogan KA,Warner GM,Tarragó MG,Peclat TR,Tchkonia T,Kirkland JL,Chini E

    更新日期：2019-05-28 00:00:00

  • Polyprenyl lipid synthesis in mammalian cells expressing human cis-prenyl transferase.

    abstract：:The level of cis-prenyl transferase activity has been implicated in controlling the level of biosynthesis of dolichol and dolichol intermediates. In this study, we isolated a cDNA encoding a human CPT (GenBank Accession No. ), which had substantial homology to other CPT isolated from human brain, bacteria, Arabidopsis...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.181

    authors： Jones J,Viswanathan K,Krag SS,Betenbaugh MJ

    更新日期：2005-06-03 00:00:00

  • The octamer motif present in the Rex-1 promoter binds Oct-1 and Oct-3 expressed by EC cells and ES cells.

    abstract：:Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer fa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2395

    authors： Rosfjord E,Rizzino A

    更新日期：1994-09-30 00:00:00

  • Intronic sequences are involved in neural targeting of human dopamine transporter gene expression.

    abstract：:Dopamine transporter (DAT) plays a key role in terminating synaptic dopaminergic transmission. DAT acts exclusively on the plasma membrane of presynaptic dopaminergic neurons and DAT gene is an appropriate model for the study of dopaminergic neuron-specific regulation of gene activity. DAT represents an important targ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7754

    authors： Kouzmenko AP,Pereira AM,Singh BS

    更新日期：1997-11-26 00:00:00

  • Impaired IL-2 expression in latent HIV-1 infection.

    abstract：:Regarding the T cell function in HIV-1 infection, activation of T cells is enhanced in acutely HIV-1-infected T cells upon stimuli. However, T cell immune responses underlying the activation of T cell receptor (TCR) signaling molecules and interleukin (IL)-2 production in latently HIV-1-infected cells are poorly under...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.06.091

    authors： Shin Y,Yoon CH,Lim H,Park J,Roh TY,Kang C,Choi BS

    更新日期：2015-08-07 00:00:00

  • Angiotensin II induces focal adhesion kinase/paxillin phosphorylation and cell migration in human umbilical vein endothelial cells.

    abstract：:In the present study, we demonstrated that Ang II provokes a transitory enhancement of focal adhesion kinase (FAK) and paxillin phosphorylation in human umbilical endothelial cells (HUVEC). Moreover, Ang II induces a time- and dose-dependent augmentation in cell migration, but does not affect HUVEC proliferation. The ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.110

    authors： Montiel M,de la Blanca EP,Jiménez E

    更新日期：2005-02-25 00:00:00

  • Proteolytic cleavages in alpha 1-antitrypsin and microheterogeneity.

    abstract：:Antitrypsin was resolved into two pools by ion-exchange chromatography. Pool 2 contained three anodal isoinhibitors and an N-terminal sequence identical with the one found by others. Pool 1 contained, in addition to the anodal ones two cathodal isoinhibitors as well. The sequencing data of Pool 1 indicate that the cat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91664-x

    authors： Hercz A

    更新日期：1985-04-16 00:00:00

  • ICOS promotes group 2 innate lymphoid cell activation in lungs.

    abstract：:Group 2 innate lymphoid cells (ILC2s) are newly identified, potent producers of type 2 cytokines, such as IL-5 and IL-13, and contribute to the development of allergic lung inflammation induced by cysteine proteases. Although it has been shown that inducible costimulator (ICOS), a costimulatory molecule, is expressed ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.06.005

    authors： Kamachi F,Isshiki T,Harada N,Akiba H,Miyake S

    更新日期：2015-08-07 00:00:00

  • Structural characterization of the novel aminoglycoside phosphotransferase AphVIII from Streptomyces rimosus with enzymatic activity modulated by phosphorylation.

    abstract：:Aminoglycoside phosphotransferases represent a broad class of enzymes that promote bacterial resistance to aminoglycoside antibiotics via the phosphorylation of hydroxyl groups in the latter. Here we report the spatial structure of the 3'-aminoglycoside phosphotransferase of novel VIII class (AphVIII) solved by X-ray ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.06.097

    authors： Boyko KM,Gorbacheva MA,Korzhenevskiy DA,Alekseeva MG,Mavletova DA,Zakharevich NV,Elizarov SM,Rudakova NN,Danilenko VN,Popov VO

    更新日期：2016-09-02 00:00:00