Angiotensin II induces focal adhesion kinase/paxillin phosphorylation and cell migration in human umbilical vein endothelial cells.

abstract：:The effect of reactive oxygen on cytosolic free calcium concentration [( Ca++]i) in pig aortic endothelial cells (ECs) was studied. Linoleate hydroperoxide (LHO) and superoxide radicals generated from xanthine with xanthine oxidase (X-XO) were used as sources of reactive oxygen. [Ca++]i in ECs was measured with quin 2...

journal_title：Biochemical and biophysical research communications

authors： Hirosumi J,Ouchi Y,Watanabe M,Kusunoki J,Nakamura T,Orimo H

更新日期：1988-04-15 00:00:00

abstract：:Genes are organized as chromatin domains and positioned in the nucleus through regions of nuclear matrix association, termed MARs. Although well-studied, the mechanisms regulating expression of the beta-globin locus remain an enigma. Here, we show that certain MAR sequences of the beta-globin locus are conserved and r...

journal_title：Biochemical and biophysical research communications

authors： Walter WR,Singh GB,Krawetz SA

更新日期：1998-01-14 00:00:00

abstract：:Bone is one of the essential target tissues of growth hormone (GH). In bone remodeling, cell-matrix attachment is important where focal adhesion kinase (FAK) is involved. FAK plays a central role in determining the shape and motility of cells in response to the extracellular matrix stimuli. In the present study, we ha...

journal_title：Biochemical and biophysical research communications

authors： Takahashi MO,Takahashi Y,Iida K,Okimura Y,Kaji H,Abe H,Chihara K

更新日期：1999-09-16 00:00:00

abstract：:The effects of various growth factors on endothelial cell survival in vitro were studied. Using rat heart endothelial cells, the cell survival curves were obtained; the cells were cultured until confluent, the medium was changed to serum-free medium with or without growth factors, and the cells were counted after 3, 6...

journal_title：Biochemical and biophysical research communications

authors： Etoh T,Takehara K,Igarashi A,Ishibashi Y

更新日期：1989-08-15 00:00:00

abstract：:1,25(OH)2D3 receptors were studied in whole testes, Sertoli cells, seminiferous tubules, Leydig cells and spermatogonia of adult NMRI mice and SD rats. Specific reversible high affinity binding (KD 1.4 x 10(-10)M; Nmax 72 fmol/mg protein) by a 3.5 S macromolecule was demonstrated in whole testes, Sertoli cells and sem...

journal_title：Biochemical and biophysical research communications

authors： Merke J,Hügel U,Ritz E

更新日期：1985-02-28 00:00:00

abstract：:In this study, we demonstrate that in addition to T lymphocytes, human naïve eosinophils and the differentiated eosinophil-like cell line, AML14.3D10 express CCR8 and respond to CCL1 through CCR8 engagement. The responsiveness of cells was dependent on maturation stage, since CCL1 induced pronounced chemotaxis only in...

journal_title：Biochemical and biophysical research communications

authors： Karlsson AK,Walles K,Bladh H,Connolly S,Skrinjar M,Rosendahl A

更新日期：2011-04-22 00:00:00

abstract：:miRNA is increasingly being recognized as a key regulator of metabolism in animals. A wealth of evidence has suggested that miRNA mainly binds 3' UTR of mRNA and modulates the cell activities via repressing the mRNA translation. However, as the translation initiates at 5' UTR, cis elements like upstream open reading f...

journal_title：Biochemical and biophysical research communications

authors： Hu W,Wang T,Yang Y,Zheng S

更新日期：2014-09-12 00:00:00

abstract：:With the global pandemic of hepatitis B and C infections, the incidence of Hepatocellular carcinoma (HCC) is rapidly increasing world wide. We identified glypican-3 (GPC3), a novel oncofetal gene over-expressed specifically in human HCC, as based on data of cDNA microarrays. As GPC3 is a GPI-anchored membrane protein ...

journal_title：Biochemical and biophysical research communications

authors： Nakatsura T,Yoshitake Y,Senju S,Monji M,Komori H,Motomura Y,Hosaka S,Beppu T,Ishiko T,Kamohara H,Ashihara H,Katagiri T,Furukawa Y,Fujiyama S,Ogawa M,Nakamura Y,Nishimura Y

更新日期：2003-06-20 00:00:00

abstract：:Topical nuclear magnetic resonance spectroscopy (NMR) using a solenoid transmit/receiver coil has been used to monitor the kinetics of intragastric utilization of [1-13C] glucose in mice in vivo. Using a double-tuned solenoid coil, signal to noise response was excellent and the natural abundance in vivo 13C spectra ob...

journal_title：Biochemical and biophysical research communications

authors： Field J,Irving MG,Brooks WM,Doddrell DM

更新日期：1984-09-28 00:00:00

abstract：:Era is an essential G-protein in Escherichia coli identified originally as a homologue protein to Ras (E. coli Ras-like protein). It binds to GTP/GDP and contains a low intrinsic GTPase activity. Its function remains elusive, although it has been suggested that Era is associated with the cytoplasmic membrane, cell div...

journal_title：Biochemical and biophysical research communications

authors： Sayed A,Matsuyama Si,Inouye M

更新日期：1999-10-14 00:00:00

abstract：:A new enzyme, tetrahydromethanopterin methyltransferase, which catalyzes the transfer of methyl groups from methyl-tetrahydromethanopterin to 2-mercaptoethane-sulfonate, has been found in the methane synthesizing complex of Methanobacterium thermoautotrophicum. The enzyme is oxygen sensitive and has a well defined pH ...

journal_title：Biochemical and biophysical research communications

authors： Sauer FD

更新日期：1986-04-29 00:00:00

abstract：:A better understanding of cell biology and cell-cell interactions requires three-dimensional (3-D) culture systems that more closely represent the natural structure and function of tissues in vivo. Here, we present a novel device that provides an environment for routine 3-D cell growth in vitro. We have developed a th...

journal_title：Biochemical and biophysical research communications

authors： Bokhari M,Carnachan RJ,Cameron NR,Przyborski SA

更新日期：2007-03-23 00:00:00

abstract：:The effect of vitamin A on the expression of apoAI and CIII genes in intact rats was studied. In vitamin A-deficient rats, the hepatic level of apoAI mRNA was increased and enhanced by an oral administration of excess retinoic acid(RA). A similar administration to normal rats caused an increase in the apoAI mRNA level...

journal_title：Biochemical and biophysical research communications

authors： Nagasaki A,Kikuchi T,Kurata K,Masushige S,Hasegawa T,Kato S

更新日期：1994-12-30 00:00:00

abstract：:TESK1 (testis-specific protein kinase 1) is a serine/threonine kinase, with a unique structure composed of an N-terminal protein kinase domain and a C-terminal proline-rich domain. Northern blot analysis revealed that TESK1 mRNA is predominantly expressed in testicular germ cells. We present here evidence that express...

journal_title：Biochemical and biophysical research communications

authors： Toshima J,Koji T,Mizuno K

更新日期：1998-08-10 00:00:00

abstract：:We purified the transcription antitermination complex of phi 80 (phi 80-pN complex). This complex had a molecular weight of approx. 42,000, and the subunits of this complex could be separated by SDS polyacrylamide gel electrophoresis. The complex was composed of two different polypeptides, one was the phi 80 N gene pr...

journal_title：Biochemical and biophysical research communications

authors： Kanemoto K,Miyashita T,Matsushiro A

更新日期：1990-05-16 00:00:00

abstract：:Hedgehog (Hh) signaling plays key roles in animal development and tissue homeostasis. Binding of the secreted ligand to its Ptch1 receptor triggers Hh signaling through distinct canonical or noncanonical signaling pathways. Canonical Hh signaling leads to the activation of Gli transcription factors to induce Hh target...

journal_title：Biochemical and biophysical research communications

authors： Lee H,Ko HW

更新日期：2016-11-25 00:00:00

abstract：:Two genomic DNA clones encoding part of the mouse epidermal growth factor (EGF) receptor were isolated: an 8.0 kb insert that hybridized with the human EGF receptor kinase domain; and a 2.4 kb insert that mapped between the kinase and 3' autophosphorylation domains of the human EGF receptor gene. The 2.4 kb insert was...

journal_title：Biochemical and biophysical research communications

authors： Hung MC,Thompson KL,Chiu IM,Rosner MR

更新日期：1986-12-30 00:00:00

abstract：:Nitric oxide synthase (EC 1.14.23) substrate analog inhibitors NG-monomethyl-L-Arg, NG-nitro-L-Arg, NG-nitro-L-Arg methyl ester, and aminoguanidine were examined as potential inhibitors of rat liver arginase (EC 3.5.3.1). NG-nitro-L-Arg was found to inhibit arginase catalyzed conversion of L-Arg to L-Orn at pH 7.5 wit...

journal_title：Biochemical and biophysical research communications

authors： Robertson CA,Green BG,Niedzwiecki L,Harrison RK,Grant SK

更新日期：1993-12-15 00:00:00

abstract：:Activin belongs to transforming growth factor (TGF)-β super family of growth and differentiation factors and activin pathway participated in broad range of cell process. Studies elaborated activin pathway maintain pluripotency in human stem cells and suggest that the function of activin/nodal signaling in self-renew w...

journal_title：Biochemical and biophysical research communications

authors： Liu R,Wang JH,Xu C,Sun B,Kang SO

更新日期：2016-10-28 00:00:00

abstract：:The receptors for insulin and insulin-like growth factor (IGF)-I carry intrinsic tyrosine-specific protein kinases (TPK) in their cytoplasmic domains that show 84% homology. Our previous studies using tyrosine-containing synthetic polymers (Arch. Biochem. Biophys. 260, 416, 1988) revealed subtle differences between th...

journal_title：Biochemical and biophysical research communications

authors： Fujita-Yamaguchi Y,Kathuria S

更新日期：1988-12-30 00:00:00

abstract：:Gangliosides are implicated in neuronal development processes. The regulation of ganglioside levels is closely related to the induction of neuronal cell differentiation. In this study, the relationship between ganglioside expression and neuronal cell development was investigated using an in vitro model of neural diffe...

journal_title：Biochemical and biophysical research communications

authors： Lee DH,Koo DB,Ko K,Ko K,Kim SM,Jung JU,Ryu JS,Jin JW,Yang HJ,Do SI,Jung KY,Choo YK

更新日期：2007-10-19 00:00:00

abstract：:We have developed a multi-target cell tracking program TADOR, which we applied to a series of fluorescence images. TADOR is based on an active contour model that is modified in order to be free of the problem of locally optimal solutions, and thus is resistant to signal fluctuation and morphological changes. Due to ad...

journal_title：Biochemical and biophysical research communications

authors： Kurokawa H,Noda H,Sugiyama M,Sakaue-Sawano A,Fukami K,Miyawaki A

更新日期：2012-01-20 00:00:00

abstract：:Rabbit brain synaptosomes bind the 1,4-dihydropyridine derivative (+)[3H]-PN 200-110 with an equilibrium dissociation constant of 0.04 nM and a maximal binding capacity of 400 fmol/mg of protein. Using polyclonal antibodies raised against the different components of the skeletal muscle 1,4-dihydropyridine receptor, we...

journal_title：Biochemical and biophysical research communications

authors： Schmid A,Barhanin J,Mourre C,Coppola T,Borsotto M,Lazdunski M

更新日期：1986-09-30 00:00:00

abstract：:Incubation of purified bovine photoreceptor rod outer segments with [gamma-32P]ATP resulted in the labeling of phosphatidylinositol 4-phosphate (PIP) and phosphatidic acid (PA) with little labeling of phosphatidylinositol 4,5-bisphosphate (PIP2). Propranolol inhibited in a dose-dependent manner the labeling of PA and ...

journal_title：Biochemical and biophysical research communications

authors： Van Rooijen LA,Bazan NG

更新日期：1986-01-14 00:00:00

abstract：:We report the usefulness of an impedance-based label-free whole cell assay to identify new ligands for G protein-coupled receptors (GPCRs) involved in microglial cell migration. Authentic GPCR ligands were subjected to the impedance-based cell assay in order to examine the responses of ligands for MG5 mouse microglial...

journal_title：Biochemical and biophysical research communications

authors： Fukano Y,Okino N,Furuya S,Ito M

更新日期：2016-09-16 00:00:00

abstract：:Considering the dynamic nature of CYPs, methods that reveal information about substrate and enzyme dynamics are necessary to generate predictive models. To compare substrate dynamics in CYP2E1 and CYP2A6, intramolecular isotope effect experiments were conducted, using deuterium labeled substrates: o-xylene, m-xylene, ...

journal_title：Biochemical and biophysical research communications

authors： Harrelson JP,Henne KR,Alonso DO,Nelson SD

更新日期：2007-01-26 00:00:00

abstract：:The influence of various porphyrins (deuteroporphyrin IX, mesoporphyrin IX, protoporphyrin IX, hematoporphyrin) and two related compounds (hemin, biliverdin) on the spontaneous proliferation of mouse spleen lymphocytes has been estimated in vitro by the 3H-thymidine uptake assay. It has been found that porphyrins (end...

journal_title：Biochemical and biophysical research communications

authors： Stepien H,Kunert-Radek J,Stanisz A,Zerek-Melen G,Pawlikowski M

更新日期：1991-01-15 00:00:00

abstract：:The objective of the present study is to demonstrate that a newly developed selective c-Fos/activator protein (AP)-1 inhibitor, T-5224, inhibits the expression of matrix metalloproteinases (MMPs) in human articular chondrocytes, and prevents cartilage destruction in an osteoarthritis (OA)-induced mouse model. First, w...

journal_title：Biochemical and biophysical research communications

authors： Motomura H,Seki S,Shiozawa S,Aikawa Y,Nogami M,Kimura T

更新日期：2018-03-04 00:00:00

abstract：:Ribosome-inactivating proteins (RIPs) are N-glycosidases (EC3.2.2.22) that universally inactivate the ribosome, thereby inhibiting protein biosynthesis. In this study, a novel type I RIPs named SEBIN was identified in Sapium sebiferum. Nuclear acid depurine experiment showed that SEBIN had rRNA N-Glycosidase activity....

journal_title：Biochemical and biophysical research communications

authors： Wu Y,Mao Y,Jin S,Hou J,Du H,Yang M,Wu L

更新日期：2015-08-07 00:00:00

abstract：:We report the initial results of our study into the use of a potential transition state (TS) of the reaction catalyzed by the enzyme estrone sulfatase (ES) in the design of a series of simple 4'-O-sulfamoyl-4-biphenyl-based compounds as novel inhibitors of ES. The results of the study show that these compounds are: po...

journal_title：Biochemical and biophysical research communications

authors： Ahmed S,James K,Owen CP

更新日期：2002-05-31 00:00:00