Abstract:
:The effect of reactive oxygen on cytosolic free calcium concentration [( Ca++]i) in pig aortic endothelial cells (ECs) was studied. Linoleate hydroperoxide (LHO) and superoxide radicals generated from xanthine with xanthine oxidase (X-XO) were used as sources of reactive oxygen. [Ca++]i in ECs was measured with quin 2 and the value for quiescent ECs was 112 +/- 11 nM. Both LHO and X-XO increased [Ca++]i in a dose-dependent manner without accompanying the significant cellular damage. Nifedipine suppressed the increase in [Ca++]i provoked by LHO and X-XO. Thus, the biological effects of reactive oxygen might be mediated, at least in part, by the activation of voltage-dependent calcium channels in ECs.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Hirosumi J,Ouchi Y,Watanabe M,Kusunoki J,Nakamura T,Orimo Hdoi
10.1016/s0006-291x(88)80714-9subject
Has Abstractpub_date
1988-04-15 00:00:00pages
301-7issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(88)80714-9journal_volume
152pub_type
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