Selective inhibition of restriction endonuclease cleavage by DNA intercalators.

Abstract:

:The preferred dye binding sites and the microenvironment of known nucleotide sequences within mitochondrial and plasmid pBR322 DNA was probed in a gross fashion with restriction endonucleases. The intercalating dyes, ethidium bromide and propidium iodide, do not inhibit a given restriction endonuclease equally at all of the restriction sites within a DNA molecule. The selective inhibition may be explained, in part, by the potential B to Z conformation transition of DNA flanking the restriction site and by preferred dye binding sites. Propidium iodide was found to be a more potent inhibitor than ethidium bromide and the inhibition is independent of the type of cut made by the enzyme.

authors

Soslau G,Pirollo K

doi

10.1016/s0006-291x(83)80170-3

subject

Has Abstract

pub_date

1983-09-15 00:00:00

pages

484-91

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(83)80170-3

journal_volume

115

pub_type

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