Regulation of adiponectin receptor R1 and R2 gene expression in adipocytes of C57BL/6 mice.

abstract：:The effects of n-3 polyunsaturated fatty acids (n-3PUFA) on obesity and diabetes were examined using KK-Ay mice fed with perilla oil (P), soybean oil (S), or lard (L), and those containing 30% fish oil (PF, SF, or LF), containing eicosapentaenoic acid (EPA = 9.9%) and docosahexaenoic acid (DHA = 18.0%). Perilla oil co...

journal_title：Biochemical and biophysical research communications

authors： Hun CS,Hasegawa K,Kawabata T,Kato M,Shimokawa T,Kagawa Y

更新日期：1999-05-27 00:00:00

abstract：:The small GTPase Rheb binds and activates mTORC1, which plays a pivotal role in diverse cellular physiologies. To increase our understanding of how Rheb regulates mTORC1 signaling, we set out to identify Rheb binding proteins using shotgun proteomics approaches. In this study, we characterized HSP70, one of the identi...

journal_title：Biochemical and biophysical research communications

authors： Ryu HH,Ha SH

更新日期：2020-12-17 00:00:00

abstract：:In pulse-chase experiments ([35S]Met as radioactive label) 4 intracellular forms of the alpha-subunit (apparent molecular weights of 11, 16.5, 19.5, and 23.4 kDa) were observed whereas almost no label was incorporated into the beta-subunit. The 23.4 kDa form was secreted as free alpha-subunit, the others were precurso...

journal_title：Biochemical and biophysical research communications

authors： Hilf G,Merz WE

更新日期：1989-02-28 00:00:00

abstract：:Lp-A-I was isolated by immunoaffinity chromatography and then separated into two fractions of large and small Lp-A-I particles by conventional ultracentrifugation with a cut-off density of 1.125 g/ml. The large and small particle-rich fractions were then radiolabeled with [125I]-Na and [131I]-Na, respectively. Both of...

journal_title：Biochemical and biophysical research communications

authors： Saku K,Liu R,Ohta T,Jimi S,Matsuda I,Arakawa K

更新日期：1994-04-15 00:00:00

abstract：:MicroRNA (miRNA) plays a pivotal role in cancer biology. Therefore, tumor suppressor (TS) miRNAs are an attractive target for cancer therapy. However, clinical trials have failed due to the difficulties in miRNA delivery, warranting the development of a novel drug delivery system (DDS). Exosomes are stable in circulat...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi M,Sawada K,Miyamoto M,Shimizu A,Yamamoto M,Kinose Y,Nakamura K,Kawano M,Kodama M,Hashimoto K,Kimura T

更新日期：2020-06-18 00:00:00

abstract：:A critical aspect of sexual development in Dictyostelium is the selection of "self" as a targeted food source by the phagocytic zygotic giant cell (ZGC). It has previously been demonstrated that phagocytic giant cells preferentially take up amoebae of the same species suggesting that the process is mediated by a speci...

journal_title：Biochemical and biophysical research communications

authors： Lewis KE,Jamieson JC

更新日期：1997-01-23 00:00:00

abstract：:Pentamannose 6-phosphate/trilysine substituted aprotinin (PMP-lys-aprotinin) and insulin like growth factor II (IGF II) were used as affinity ligands for the mannose 6-phosphate (M6P) and IGF II binding sites of the M6P/IGF II receptor. Both ligands were cross linked to intact receptor and tryptic fragments of the rec...

journal_title：Biochemical and biophysical research communications

authors： Waheed A,Braulke T,Junghans U,von Figura K

更新日期：1988-05-16 00:00:00

abstract：:Yes-associated protein (YAP) is a key downstream effector of the highly conserved Hippo signaling pathway, which regulates organ size, regeneration and tumorigenesis. Known classically to function as a transcriptional co-activator, YAP interacts with TEA domain transcription factors (TEAD1-4) to induce expression of t...

journal_title：Biochemical and biophysical research communications

authors： Wen Z,Wang Y,Qi S,Ma M,Li J,Yu FX

更新日期：2020-10-15 00:00:00

abstract：:Traditional reconstitution of membrane cytochromes P450 monooxygenase system requires efficient solubilization of both P450 heme enzymes and redox partner NADPH dependent reductase, CPR, either in mixed micellar solution or by incorporation in liposomes. Here we describe a simple alternative approach to assembly of so...

journal_title：Biochemical and biophysical research communications

authors： Grinkova YV,Denisov IG,Sligar SG

更新日期：2010-07-23 00:00:00

abstract：:A sample of highly oriented Na-hyaluronate was prepared with a wet--spinning method and equilibrated to 75% relative humidity. Its proton NMR spectrum was recorded as a function of the angle theta between the hyaluronate helix axis and the external magnetic field. It appeared as a singlet with an angle-dependent line ...

journal_title：Biochemical and biophysical research communications

authors： Lahajnar G,Rupprecht A

更新日期：1986-11-26 00:00:00

abstract：:We investigated the role of tyrosine phosphorylation of FAK in the stretch-induced MAPKs (extracellular signal-regulated kinase (ERK), p38MAPK) activation in mutant FAK-transfected fibroblasts. In response to uniaxial cyclic stretch (1 Hz, 120% in length), the levels of tyrosine phosphorylation of the Tyr-397 and Tyr-...

journal_title：Biochemical and biophysical research communications

authors： Wang JG,Miyazu M,Matsushita E,Sokabe M,Naruse K

更新日期：2001-10-26 00:00:00

abstract：:Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted ...

journal_title：Biochemical and biophysical research communications

authors： Korc M,Matrisian LM,Planck SR,Magun BE

更新日期：1983-03-29 00:00:00

abstract：:Growing evidence indicates that circular RNA (circRNA) plays an important role in the regulation of tumor biological behaviors. In this study, we aimed to explore the role of a novel circRNA, circ_0034642, in glioma. qRT-PCR was conducted to evaluate the levels of circ_0034642 in glioma tissues and cells. In addition,...

journal_title：Biochemical and biophysical research communications

authors： Yang M,Li G,Fan L,Zhang G,Xu J,Zhang J

更新日期：2019-01-15 00:00:00

abstract：:Mitogen-activated protein kinases (MPKs) are involved in a number of signaling pathways that control plant development and stress tolerance via the phosphorylation of target molecules. However, so far only a limited number of target molecules have been identified. Here, we provide evidence that MYB41 represents a new ...

journal_title：Biochemical and biophysical research communications

authors： Hoang MH,Nguyen XC,Lee K,Kwon YS,Pham HT,Park HC,Yun DJ,Lim CO,Chung WS

更新日期：2012-05-25 00:00:00

abstract：:Using a PCR-based cloning strategy we have isolated a cDNA from mouse brain and named it fex, because it codes for a novel putative G protein-coupled receptor expressed in follicles. The deduced amino acid sequence shows a higher degree of homology to the family of glycoprotein receptors, namely those for FSH, LH, and...

journal_title：Biochemical and biophysical research communications

authors： Hermey G,Methner A,Schaller HC,Hermans-Borgmeyer I

更新日期：1999-01-08 00:00:00

abstract：:A new and simple method for the detection of antigenic proteins blotted onto nitrocellulose was developed. After transfer of spinach stromal proteins and purified phosphoribulokinase immunolabeling was performed with phosphoribulokinase antiserum, followed by a) Protein A-labeled colloidal gold particles, and b) by ho...

journal_title：Biochemical and biophysical research communications

authors： Surek B,Latzko E

更新日期：1984-05-31 00:00:00

abstract：:Transient receptor potential (TRP) proteins are cation channels that comprise a superfamily of molecular sensors that enable animals to detect a wide variety of environmental stimuli. This versatility enables vertebrate and invertebrate TRP channels to function in a diversity of senses, ranging from vision to taste, s...

journal_title：Biochemical and biophysical research communications

authors： Liu C,Montell C

更新日期：2015-04-24 00:00:00

abstract：:Although it's well known that protein carbonyl (PCO) and advanced glycation end-products (AGEs) levels are elevated in plasma from patients with renal dysfunction, we recently identified patients who had no renal dysfunction but possessed high levels of plasma pentosidine (PEN), which is an AGEs, and low vitamin B6 le...

journal_title：Biochemical and biophysical research communications

authors： Koike S,Kayama T,Arai M,Horiuchi Y,Kobori A,Miyashita M,Itokawa M,Ogasawara Y

更新日期：2015-11-13 00:00:00

abstract：:Stimulation of cultured hypothalamic slices with PRL causes a rapid translocation of a Ca2+/phospholipid dependent protein kinase from the cytosol to the membrane fraction. The translocation of PKC from the cytosol to the membrane occurred at physiological concentrations of PRL with a maximal response occurring at 10(...

journal_title：Biochemical and biophysical research communications

authors： DeVito WJ,Stone S,Avakian C

更新日期：1991-04-30 00:00:00

abstract：:Using the primary culture of neonatal rat ventricular myocytes, synthesis and secretion of rat atrial natriuretic peptide (rANP) were studied. Ventricular myocytes in culture, although contained less amounts of cellular immunoreactive (IR)-rANP, secreted substantial amounts of IR-rANP at a rate comparable to that of a...

journal_title：Biochemical and biophysical research communications

authors： Matsubara H,Hirata Y,Yoshimi H,Takata S,Takagi Y,Yamane Y,Umeda Y,Nishikawa M,Inada M

更新日期：1987-11-13 00:00:00

abstract：:S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTases) methylate diverse biological molecules using a SAM cofactor. The ytqB gene of Bacillus subtilis encodes a putative MTase and its biological function has never been characterized. To reveal the structural features and the cofactor binding mode of YtqB,...

journal_title：Biochemical and biophysical research communications

authors： Park SC,Song WS,Yoon SI

更新日期：2014-04-18 00:00:00

abstract：:Glycogen phosphorylase (a or b) binds 125I-calmodulin in a Ca2+-dependent manner, in the 125I-calmodulin overlay technique. This binding is quantitatively identical to 125I-calmodulin binding to myosin light chain kinase. In an in vitro assay, calmodulin stimulates phosphorylase activity at limiting concentrations of ...

journal_title：Biochemical and biophysical research communications

authors： Slaughter GR,Means AR

更新日期：1985-01-16 00:00:00

abstract：:The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)(24) and 5'-(dGdC)(18), was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right...

journal_title：Biochemical and biophysical research communications

authors： D'Urso A,Choi JK,Shabbir-Hussain M,Ngwa FN,Lambousis MI,Purrello R,Balaz M

更新日期：2010-06-25 00:00:00

abstract：:To characterize mesenchymal stem cells (MSC), we compared gene expression profiles in human bone marrow MSC (11 lines) and human fibroblasts (4 lines) by RT-PCR and real time PCR. Messenger RNA levels of MHC-DR-alpha, MHC-DR-beta, MHC-DR-associated protein CD74, tissue factor pathway inhibitor-2, and neuroserpin were ...

journal_title：Biochemical and biophysical research communications

authors： Ishii M,Koike C,Igarashi A,Yamanaka K,Pan H,Higashi Y,Kawaguchi H,Sugiyama M,Kamata N,Iwata T,Matsubara T,Nakamura K,Kurihara H,Tsuji K,Kato Y

更新日期：2005-06-24 00:00:00

abstract：:Epithelial cells form a globular organ-like multi-cellular structure called cyst when cultured in extracellular matrix. The cyst generates extension followed by cell chains and tubules in response to hepatocyte growth factor (HGF). The Rho family small GTPases play essential roles for tubulogenesis. FilGAP, a Rac spec...

journal_title：Biochemical and biophysical research communications

authors： Zuinen T,Tsutsumi K,Ohta Y

更新日期：2019-06-30 00:00:00

abstract：:RNA was isolated from bean leaves with various iron and ferritin contents. The amount of translatable ferritin mRNA in these RNAs was determined by wheat-germ cell-free translation and immunoprecipitation with anti-bean seed ferritin serum. The levels of ferritin mRNA present in total RNA of the leaves are reflected b...

journal_title：Biochemical and biophysical research communications

authors： van der Mark F,Bienfait F,van den Ende H

更新日期：1983-09-15 00:00:00

abstract：:We report the initial results of our study into the use of a potential transition state (TS) of the reaction catalyzed by the enzyme estrone sulfatase (ES) in the design of a series of simple 4'-O-sulfamoyl-4-biphenyl-based compounds as novel inhibitors of ES. The results of the study show that these compounds are: po...

journal_title：Biochemical and biophysical research communications

authors： Ahmed S,James K,Owen CP

更新日期：2002-05-31 00:00:00

abstract：:We describe a new method for multi-wavelength immunoassays using surface plasmon-coupled emission (SPCE). This phenomenon is coupling of excited fluorophores with a nearby thin metal film, in our case silver, resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiati...

journal_title：Biochemical and biophysical research communications

authors： Matveeva E,Malicka J,Gryczynski I,Gryczynski Z,Lakowicz JR

更新日期：2004-01-16 00:00:00

abstract：:In peplomycin-supersensitive Chinese hamster lung cells, the increase in poly(ADP-ribose) synthesizing activity following peplomycin treatment was significantly reduced as compared with the parental lung cells, suggesting that peplomycin-supersensitive lung cells may have some deficiency in DNA repair. On the contrary...

journal_title：Biochemical and biophysical research communications

authors： Ozawa S,Suzuki H,Yamaki H,Nishimura T,Tanaka N

更新日期：1988-03-30 00:00:00

abstract：:Soluble guanylyl cyclase (sGC) is activated by nitric oxide (NO) and generates the second messenger cyclic GMP (cGMP). Recently, purified sGC α1β1 has been shown to additionally generate the cyclic pyrimidine nucleotides cCMP and cUMP. However, since cyclic pyrimidine nucleotide formation occurred only the presence of...

journal_title：Biochemical and biophysical research communications

authors： Bähre H,Danker KY,Stasch JP,Kaever V,Seifert R

更新日期：2014-01-24 00:00:00