Abstract：
:A critical aspect of sexual development in Dictyostelium is the selection of "self" as a targeted food source by the phagocytic zygotic giant cell (ZGC). It has previously been demonstrated that phagocytic giant cells preferentially take up amoebae of the same species suggesting that the process is mediated by a specific receptor. By using tunicamycin, which inhibits N-linked glycosylation and mevastatin and mevinolin, which both inhibit HMG-CoA-reductase, we have been able to further characterize the glycoprotein(s) involved in the recognition process. We have utilized a "cell blotting" technique which lends itself well to identifying glycoproteins which are present on the ZGC at the phagocytic stage and interactive with the target amoebae. The cell blotting data, combined with pharmacological evidence, identifies these glycoproteins as the receptors for cannibalistic phagocytosis by the ZGC of D. discoideum.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Lewis KE,Jamieson JCdoi
10.1006/bbrc.1996.5990subject
Has Abstractpub_date
1997-01-23 00:00:00pages
505-8issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(96)95990-2journal_volume
230pub_type
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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