Abstract:
:Using the primary culture of neonatal rat ventricular myocytes, synthesis and secretion of rat atrial natriuretic peptide (rANP) were studied. Ventricular myocytes in culture, although contained less amounts of cellular immunoreactive (IR)-rANP, secreted substantial amounts of IR-rANP at a rate comparable to that of atrial myocytes. Dexamethasone markedly stimulated synthesis and secretion of IR-rANP by cultured ventricular myocytes in a dose-dependent manner (10(-10)-10(-6) M), of which effect was far more potent than that in atrial myocytes. Testosterone and triiodothyronine also stimulated synthesis and secretion of ventricular IR-rANP to the extent comparable to that of atrial IR-rANP. The present study suggests that tissue-dependent difference in glucocorticoids sensitivity plays an important role in the regulation of developmental ANP gene expression in mammalian heart.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Matsubara H,Hirata Y,Yoshimi H,Takata S,Takagi Y,Yamane Y,Umeda Y,Nishikawa M,Inada Mdoi
10.1016/s0006-291x(87)80235-8subject
Has Abstractpub_date
1987-11-13 00:00:00pages
1030-8issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(87)80235-8journal_volume
148pub_type
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