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Accumulation of deletions in MtDNA during tissue aging: analysis by long PCR.

Abstract：

:Multiple deletions of mtDNA have not only been implicated in aging, but also in a wide variety of pathological conditions. The enzyme system used in long-PCR makes it possible to synthesize the entire mitochondrial genome (16.5 kb), exposing the multiple deletions in mtDNAs implicated in and, at least partially, responsible for these pathologies. But it is not the number or type of anomalous mtDNA that is crucial, rather it is their frequency relative to the number of intact copies of the mitochondrial genome. Our work exposes the necessity of quantitating the number of normal mitochondrial DNAs. The accuracy of the technique and the small sample size required permit one to detect multiple deletions, located in a specific organ, and simultaneously measure the fraction of intact molecules. This fraction can then be correlated with mitochondrial dysfunction to serve both as an indicator of tissue aging and a monitor of an impending myopathy.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Reynier P,Malthiery Y

doi

10.1006/bbrc.1995.2745

subject

Has Abstract

pub_date

1995-12-05 00:00:00

pages

59-67

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(85)72745-3

journal_volume

217

pub_type

杂志文章

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