Abstract:
:Ca2+-phospholipid dependent protein kinase activity and the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on H+ secretion was studied in guinea-pig parietal cells. In resting parietal cells PK-C activity was distributed almost equally in both the cytosolic and particulate cell fraction. Exposure of the cells to TPA resulted in a loss of cytosolic PK-C activity which was not accompanied by a concomitant increase of particulate activity. Furthermore TPA inhibits the K+/H+-ATPase and dissipates H+ gradient in intact gastric vesicles by a protonophoric action. The role of these biochemical events for the antisecretory action of TPA found in the intact parietal cell are discussed.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Beil W,Mannschedel W,Sewing KFdoi
10.1016/0006-291x(87)90427-xsubject
Has Abstractpub_date
1987-12-16 00:00:00pages
720-8issue
2eissn
0006-291Xissn
1090-2104pii
0006-291X(87)90427-Xjournal_volume
149pub_type
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