当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Gaussian FRET two-hybrid assays for determining the stoichiometry of hetero-oligomeric complexes in single living cells.

Gaussian FRET two-hybrid assays for determining the stoichiometry of hetero-oligomeric complexes in single living cells.

Abstract：

:Here we integrate multiple Gaussian-functions analysis into fluorescence resonance energy transfer (FRET) two-hybrid assays (Gaussian FRET two-hybrid assay) to determine the stoichiometric ratios of intracellular hetero-oligomers in single living cells. This method adopts in multiple Gaussian-functions to fit the E-count histograms of both donor- and acceptor-centric FRET efficiency (ED and EA) images of a single cell for obtaining the peak values (EDi and EAi), thus yielding the corresponding stoichiometric ratios (EDi/EAi) of intracellular hetero-oligomers. We performed Gaussian FRET two-hybrid assay for living Hela cells coexpressing different FRET tandem plasmids, and obtained consistent results with the expected values. Gaussian FRET two-hybrid assay for cells coexpressing Bad-CFP and Bcl-XL-YFP reveals that Bcl-XL binds with Bad to form a hetero-oligomeric complex with a stoichiometry of 2:1 on mitochondria.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Qu W,Du M,Yang F,Mai Z,Zhang C,Lin F,Ma Y,Chen T

doi

10.1016/j.bbrc.2019.03.089

subject

Has Abstract

pub_date

2019-05-07 00:00:00

pages

492-497

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(19)30478-4

journal_volume

512

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Long-lasting vasoconstriction and efficient regional extraction of endothelin-1 in human splanchnic and renal tissues.

    abstract：:Six healthy subjects were given endothelin-1, intravenously in a dose of 4 pmol.kg-1.min-1 for 20 min. Blood samples were drawn from arterial, hepatic and renal vein catheters for determinations of splanchnic and renal blood flows and the extraction of endothelin-1 in these vascular beds. Intravenous infusion of endot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81336-1

    authors： Weitzberg E,Ahlborg G,Lundberg JM

    更新日期：1991-11-14 00:00:00

  • A larval specific lipoprotein: purification and characterization of a blue chromoprotein from Heliothis zea.

    abstract：:The green color of the hemolymph of last instar larvae of the corn earworm, Heliothis zea (Boddie), is caused by the appearance of a blue chromoprotein. This protein (Mr approximately 560,000) is composed of 4 subunits (Mr approximately 150,000). It contains 8.4% lipid and has an equilibrium density of 1.26 g/ml. This...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80459-4

    authors： Haunerland NH,Bowers WS

    更新日期：1986-01-29 00:00:00

  • Stabilization of P/CAF, as a ubiquitin ligase toward MDM2, suppresses mitotic cell death through p53-p21 activation in HCT116 cells with SIRT2 suppression.

    abstract：:We previously reported that the suppression of SIRT2, an NAD + -dependent protein deacetylases, induces p53 accumulation via degradation of p300 and the subsequent MDM2 degradation, eventually leading to apoptosis in HeLa cells. The present study identified a novel pathway of p53 accumulation by SIRT2 suppression in H...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.11.136

    authors： Li Y,Kokura K,Inoue T

    更新日期：2019-01-01 00:00:00

  • D-galactosyl-beta1-1'-sphingosine and D-glucosyl-beta1-1'-sphingosine induce human natural killer cell apoptosis.

    abstract：:Natural killer (NK) cells perform multiple biological functions including tumor cell lysis and eradicating virally infected cells. Here, we report for the first time that D-galactosyl-beta1-1' sphingosine and D-glucosyl-beta1- 1' sphingosine damage human NK cells. We show that these cells express T-cell-associated gen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.027

    authors： Maghazachi AA,Knudsen E,Jin Y,Jenstad M,Chaudhry FA

    更新日期：2004-07-30 00:00:00

  • Metabolism of lipoxins A4 and B4 and of their all-trans isomers by human leukocytes and rat liver microsomes.

    abstract：:Incubation of lipoxin A4 (LXA4) either with human leukocytes or with rat liver microsomes in the presence of NADPH very selectively led to a more polar metabolite retaining the conjugated tetraenic structure of LXA4. Lipoxin B4 (LXB4) underwent a very similar metabolism into a more polar metabolite, whereas the all-tr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91958-f

    authors： Boucher JL,Delaforge M,Mansuy D

    更新日期：1991-05-31 00:00:00

  • Down-regulated expression of exocytotic proteins in pancreatic islets of diabetic GK rats.

    abstract：:Exocytosis is regulated by exocytotic proteins, which are present in insulin-secreting beta-cells and play regulatory roles in insulin secretion. Non-insulin dependent diabetes mellitus (type 2 diabetes) is a disease characterized by impaired insulin secretion and insulin resistance. Exocytotic protein immunoreactivit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6555

    authors： Zhang W,Khan A,Ostenson CG,Berggren PO,Efendic S,Meister B

    更新日期：2002-03-08 00:00:00

  • Thiol levels in CD134-defined subsets of rat T lymphocytes: possible implications for HgCl2-induced immune dysregulation.

    abstract：:CD134 (OX40), a member of the tumour necrosis factor receptor family, is expressed on activated T cells and mediates T and B cell costimulation. Its expression is increased after exposure to the thiol-binding compound HgCl2 in BN rats, but not in Lewis rats, in association with induction of a T cell-dependent systemic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7679

    authors： Roos A,Claessen N,Schilder-Tol EJ,Chand MA,Weening JJ,Aten J

    更新日期：1997-11-17 00:00:00

  • Diacylglycerol causes major structural transitions in phospholipid bilayer membranes.

    abstract：:We demonstrate for the first time that major structural changes are imposed on various phospholipid bilayers by diacylglycerol, a product of phosphatidylinositol metabolism. By 5 mole percent in phosphatidylethanolamine a lamellar to hexagonal transition starts that is complete at 10 mole percent. At 30 mole percent i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91580-8

    authors： Das S,Rand RP

    更新日期：1984-10-30 00:00:00

  • Jmjd2c histone demethylase enhances the expression of Mdm2 oncogene.

    abstract：:Jmjd2c is a candidate oncogene that encodes histone lysine demethylase. In this study, we discovered that over-expression of Jmjd2c increased the expression of Mdm2 oncogene dependent on its demethylase activity, which led to the reduction of p53 tumor suppressor gene product in the cells. A chromatin immunoprecipitat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.08.155

    authors： Ishimura A,Terashima M,Kimura H,Akagi K,Suzuki Y,Sugano S,Suzuki T

    更新日期：2009-11-13 00:00:00

  • Inhibition of platelet adhesion to collagen by cGMP-elevating agents.

    abstract：:In the present study, we examined the effects and action mechanisms of cGMP-elevating agents on platelet adhesion to collagen fiber. YC-1, a nitric oxide (NO)-independent activator of soluble guanylate cyclase, inhibited both initial and long-term platelet adhesion to collagen, and the inhibitory effect was potentiate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.5998

    authors： Wu CC,Ko FN,Teng CM

    更新日期：1997-02-13 00:00:00

  • S1P(2) receptors mediate inhibition of glioma cell migration through Rho signaling pathways independent of PTEN.

    abstract：:Sphingosine 1-phosphate (S1P) induced the inhibition of glioma cell migration. Here, we characterized the signaling mechanisms involved in the inhibitory action by S1P. In human GNS-3314 glioblastoma cells, the S1P-induced inhibition of cell migration was associated with activation of RhoA and suppression of Rac1. The...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.12.054

    authors： Malchinkhuu E,Sato K,Maehama T,Mogi C,Tomura H,Ishiuchi S,Yoshimoto Y,Kurose H,Okajima F

    更新日期：2008-02-22 00:00:00

  • Trophoblast lineage cells derived from human induced pluripotent stem cells.

    abstract：BACKGROUND:During implantation, the blastocyst trophectoderm attaches to the endometrial epithelium and continues to differentiate into all trophoblast subtypes, which are the major components of a placenta. Aberrant trophoblast proliferation and differentiation are associated with placental diseases. However, due to e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.06.016

    authors： Chen Y,Wang K,Chandramouli GV,Knott JG,Leach R

    更新日期：2013-07-12 00:00:00

  • Antitumor effect of the integrin α4 signaling inhibitor JK273 in non-small cell lung cancer NCI-H460 cells.

    abstract：:Lung cancer accounts for the highest death rate among cancers worldwide, with most patients being diagnosed with non-small cell lung cancer (NSCLC), urging more effective therapies. We report that JK273, a pyrrolo[2,3-d]pyrimidine analog, which inhibits α4 integrin signaling, showed a selective cytotoxic effect agains...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.07.096

    authors： Lu TN,Ganganna B,Pham TT,Vo AV,Lu TP,Nguyen HT,Nguyen MT,Huynh PN,Truong NT,Lee J

    更新日期：2017-09-16 00:00:00

  • Mdm2 is required for HDAC3 monoubiquitination and stability.

    abstract：:HDAC3, one of the class I histone deacetylase modulates epigenetic landscape through histone modification. HDAC3 also interacts with non-histone proteins including p53 for deacetylation. Moreover, HDAC3 serves as a transcriptional repressor, interacting with NCor1/SMRT complex. Although HDAC3 plays a critical role for...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.07.052

    authors： Choi YM,An S,Bae S,Jung JH

    更新日期：2019-09-17 00:00:00

  • Knockout of neutrophil elastase protects against western diet induced nonalcoholic steatohepatitis in mice by regulating hepatic ceramides metabolism.

    abstract：:Previous studies reported increased expression and activity of neutrophil elastase (NE) in NASH. However, the role of NE in nonalcoholic steatohepatitis (NASH) remained unclear. Wild type (WT) and NE knockout (NE KO) mice were fed with western diet (WD) for 24 weeks to establish NASH model. The severity of liver injur...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.08.111

    authors： Chen J,Liang B,Bian D,Luo Y,Yang J,Li Z,Zhuang Z,Zang S,Shi J

    更新日期：2019-10-22 00:00:00

  • Degradation of dynorphin-(1-13) and dynorphin-(1-17) by the neuroblastoma cell membrane. Evidence for the involvement of a cysteine protease.

    abstract：:The membrane of mouse neuroblastoma N-18 cells degraded dynorphin-(1-13), dynorphin-(1-17), and Leu-enkephalin. The degradation of the former two peptides was inhibited strongly by N-ethylmaleimide, moderately by diisopropylphosphorofluoridate and phosphoramidon, and slightly by bestatin. When Leu-enkephalin was the s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91095-8

    authors： Satoh M,Yokosawa H,Ishii S

    更新日期：1986-10-15 00:00:00

  • Bioactive synthetic peptide of NDF/heregulin.

    abstract：:A folded synthetic peptide analog of NDF (NDF5), which has the 52 amino acid EGF-like domain of NDF alpha 2, has been characterized. The folded peptide stimulates tyrosine phosphorylation of Her2, Her3 and Her4 in breast cancer cells and competes with low affinity with full-length NDF alpha 2 for binding to the cells,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1680

    authors： Kita Y,Mayer J,Zamborelli T,Hara S,Rohde M,Watson E,Koski R,Ratzkin B,Nicolson M

    更新日期：1995-05-16 00:00:00

  • Stoichiometry of ATP hydrolysis and chlorophyllide formation of dark-operative protochlorophyllide oxidoreductase from Rhodobacter capsulatus.

    abstract：:Dark-operative protochlorophyllide (Pchlide) oxidoreductase (DPOR) is a nitrogenase-like enzyme catalyzing a reduction of the C17 = C18 double bond of Pchlide to form chlorophyllide a (Chlide) in bacteriochlorophyll biosynthesis. DPOR consists of an ATP-dependent reductase component, L-protein (a BchL dimer), and a ca...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.01.070

    authors： Nomata J,Terauchi K,Fujita Y

    更新日期：2016-02-12 00:00:00

  • Palmitic acid increases invasiveness of pancreatic cancer cells AsPC-1 through TLR4/ROS/NF-κB/MMP-9 signaling pathway.

    abstract：:Pancreatic cancer (PC) is an aggressive malady with proclivity for early metastasis. Overexpression of toll-like receptor 4 (TLR4) in pancreatic ductal adenocarcinoma, the most common type of pancreatic malignancy, correlates to tumor size, lymph node involvement, venous invasion and pathological stage. Lipopolysaccha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.01.051

    authors： Binker-Cosen MJ,Richards D,Oliver B,Gaisano HY,Binker MG,Cosen-Binker LI

    更新日期：2017-02-26 00:00:00

  • A method for specific cloning and sequencing of human hprt cDNA for mutation analysis.

    abstract：:A method has been developed for the specific amplification and cloning of human hprt cDNA which can be used for mutant sequence analysis. Messenger RNA is isolated from TK6 lymphoblasts and is used to produce a first strand cDNA with reverse transcriptase primed with oligo dT. Second strand synthesis and subsequent am...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90619-5

    authors： Simpson D,Crosby RM,Skopek TR

    更新日期：1988-02-29 00:00:00

  • Local database and the search program for proteomic analysis of sperm proteins in the ascidian Ciona intestinalis.

    abstract：:Separation of proteins by two-dimensional electrophoresis and following mass spectrometry (MS) is now a conventional technique for proteomic analysis. For proteomic analysis of a certain tissue with a limited information of primary structures of proteins, we have developed an analytical system for peptide mass fingerp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.05.118

    authors： Hozumi A,Satouh Y,Ishibe D,Kaizu M,Konno A,Ushimaru Y,Toda T,Inaba K

    更新日期：2004-07-09 00:00:00

  • Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.

    abstract：:CRACR2A protein is described in T cells as an EF-hand-containing modulator of calcium-release-activated calcium (CRAC) channels. Here we sought relevance to calcium entry of endothelial cells. Unexpectedly, short interfering RNA designed to deplete CRACR2A had no effect on CRAC channels in endothelial cells but reduce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.11.095

    authors： Wilson LA,McKeown L,Tumova S,Li J,Beech DJ

    更新日期：2015-01-02 00:00:00

  • Overexpression of Arabidopsis NADPH-dependent thioredoxin reductase C (AtNTRC) confers freezing and cold shock tolerance to plants.

    abstract：:Overexpression of AtNTRC (AtNTRC(OE)) in Arabidopsis thaliana led to a freezing and cold stress tolerance, whereas a knockout mutant (atntrc) showed a stress-sensitive phenotype. Biochemical analyses showed that the recombinant AtNTRC proteins exhibited a cryoprotective activity for malate dehydrogenase and lactic deh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.06.089

    authors： Moon JC,Lee S,Shin SY,Chae HB,Jung YJ,Jung HS,Lee KO,Lee JR,Lee SY

    更新日期：2015-08-07 00:00:00

  • SDF-1/CXCR4 axis promotes the growth and sphere formation of hypoxic breast cancer SP cells by c-Jun/ABCG2 pathway.

    abstract：:ATP-binding cassette sub-family G member 2 (ABCG2) confers to the major phenotypes of side population (SP) cells, the cancer stem-like cells. In this study, the SP cells displayed a distinctly higher ABCG2 expression level, sphere formation efficiency (SFE) and growth rate even under hypoxia condition. CXCR4 overexpre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.130

    authors： He C,Zhang H,Wang B,He J,Ge G

    更新日期：2018-10-28 00:00:00

  • An ABC transporter complex encoded by Aluminum Sensitive 3 and NAP3 is required for phosphate deficiency responses in Arabidopsis.

    abstract：:Phosphate is essential for cell metabolism in all organisms. As it is often limiting in the soil, plants have evolved various mechanisms to cope with low-phosphate conditions. Here, we report that Aluminum Sensitive 3 and NAP3, two genes previously identified to function in aluminum tolerance, play a critical role in ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.05.009

    authors： Belal R,Tang R,Li Y,Mabrouk Y,Badr E,Luan S

    更新日期：2015-07-17 00:00:00

  • Isolation of ATMEKK1 (a MAP kinase kinase kinase)-interacting proteins and analysis of a MAP kinase cascade in Arabidopsis.

    abstract：:In plants, a number of MAP kinase (MAPK), MAPK kinase (MAPKK), and MAPKK kinase (MAPKKK) homologues have been reported. However, there have been no reports of protein-protein interactions between these kinases or molecular analysis of MAPK cascades in higher plants. To analyze a possible MAPK cascade in Arabidopsis th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9796

    authors： Ichimura K,Mizoguchi T,Irie K,Morris P,Giraudat J,Matsumoto K,Shinozaki K

    更新日期：1998-12-18 00:00:00

  • The linker region of Smad2 mediates TGF-beta-dependent ERK2-induced collagen synthesis.

    abstract：:Transforming growth factor (TGF)-beta1 can cause fibrosis diseases by enhancing production of collagen. However, the intracellular signaling mechanism for TGF-beta1 stimulation of this process has not been fully elucidated. The present study focused on this mechanism and the cross-talk between the MAPK and Smad pathwa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.05.084

    authors： Li F,Zeng B,Chai Y,Cai P,Fan C,Cheng T

    更新日期：2009-08-21 00:00:00

  • Gastrodin inhibits osteoclastogenesis via down-regulating the NFATc1 signaling pathway and stimulates osseointegration in vitro.

    abstract：:Bone is a rigid yet dynamic organ, and this dynamism is mediated by the delicate balance between osteoclastic bone resorption and osteoblastic bone formation. However, excessive activation of osteoclasts is responsible for many bone diseases such as osteoporosis, Paget disease, and tumor bone metastasis. Agents that c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.01.179

    authors： Zhou F,Shen Y,Liu B,Chen X,Wan L,Peng D

    更新日期：2017-03-18 00:00:00

  • Efficient gene transfection by bisguanylated diacetylene lipid formulations.

    abstract：:We have previously shown that cationic cholesterol derivatives bearing guanidinium groups were efficient vectors for gene transfer. To further evaluate the potentiality of this novel class of cationic lipids, we undertook to study the transfection efficiency of guanidinium-based lipids with other hydrophobic moieties....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4401

    authors： Patel M,Vivien E,Hauchecorne M,Oudrhiri N,Ramasawmy R,Vigneron JP,Lehn P,Lehn JM

    更新日期：2001-02-23 00:00:00

  • The fourth immunoglobulin-like loop in the extracellular domain of FLT-1, a VEGF receptor, includes a major heparin-binding site.

    abstract：:We found that heparin at low concentrations increases the vascular endothelial growth factor (VEGF)-induced proliferation of human umbilical vein endothelial cells (HUVECs) but at high concentrations decreases it. To examine whether FLT-1, a VEGF receptor, interacts with heparin and which domain of FLT-1 binds to hepa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1580

    authors： Park M,Lee ST

    更新日期：1999-11-02 00:00:00