当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > ATP binding to two sites is necessary for dimerization of nucleotide-binding domains of ABC proteins.

ATP binding to two sites is necessary for dimerization of nucleotide-binding domains of ABC proteins.

Abstract：

:ATP binding cassette (ABC) transporters have a functional unit formed by two transmembrane domains and two nucleotide binding domains (NBDs). ATP-bound NBDs dimerize in a head-to-tail arrangement, with two nucleotides sandwiched at the dimer interface. Both NBDs contribute residues to each of the two nucleotide-binding sites (NBSs) in the dimer. In previous studies, we showed that the prototypical NBD MJ0796 from Methanocaldococcus jannaschii forms ATP-bound dimers that dissociate completely following hydrolysis of one of the two bound ATP molecules. Since hydrolysis of ATP at one NBS is sufficient to drive dimer dissociation, it is unclear why all ABC proteins contain two NBSs. Here, we used luminescence resonance energy transfer (LRET) to study ATP-induced formation of NBD homodimers containing two NBSs competent for ATP binding, and NBD heterodimers with one active NBS and one binding-defective NBS. The results showed that binding of two ATP molecules is necessary for NBD dimerization. We conclude that ATP hydrolysis at one nucleotide-binding site drives NBD dissociation, but two binding sites are required to form the ATP-sandwich NBD dimer necessary for hydrolysis.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Zoghbi ME,Altenberg GA

doi

10.1016/j.bbrc.2013.11.050

subject

Has Abstract

pub_date

2014-01-03 00:00:00

pages

97-102

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(13)01943-8

journal_volume

443

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Membrane-acting bacteriocin purified from a soil isolate Pediococcus pentosaceus LB44 shows broad host-range.

    abstract：:Bacteriocin LB44 was purified from cell-free supernatant (CFS) of Pediococcus pentosaceus LB44 using activity-guided chromatography techniques. It was stable up to 121 °C, pH 2.0-6.0, sensitive to proteinase K, papain and trypsin, and retained complete activity in the presence of organic solvents tested. The molecular...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.03.062

    authors： Kaur R,Tiwari SK

    更新日期：2018-04-15 00:00:00

  • Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta).

    abstract：:Intestinal N-acetylglucosamine 6-O-sulfotransferase (I-GlcNAc6ST, GST-4alpha) and corneal N-acetylglucosamine 6-O-sulfotransferases (C-GlcNAc6ST, GST-4beta) are two highly homologous GlcNAc 6-O-sulfotransferase isozymes encoded by two intronless open reading frames that reside approximately 50 kb apart on human chromo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4668

    authors： Bartes A,Bhakta S,Hemmerich S

    更新日期：2001-04-13 00:00:00

  • An ABC transporter complex encoded by Aluminum Sensitive 3 and NAP3 is required for phosphate deficiency responses in Arabidopsis.

    abstract：:Phosphate is essential for cell metabolism in all organisms. As it is often limiting in the soil, plants have evolved various mechanisms to cope with low-phosphate conditions. Here, we report that Aluminum Sensitive 3 and NAP3, two genes previously identified to function in aluminum tolerance, play a critical role in ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.05.009

    authors： Belal R,Tang R,Li Y,Mabrouk Y,Badr E,Luan S

    更新日期：2015-07-17 00:00:00

  • Inhibition of protein tyrosine phosphatases by low-molecular-weight S-nitrosothiols and S-nitrosylated human serum albumin.

    abstract：:The homogeneous recombinant mammalian protein tyrosine phosphatase 1B (PTP1B) and Yersinia protein tyrosine phosphatase (PTPase) are inactivated by a series of low-molecular-weight S-nitrosothiols. These compounds exhibited different inhibitory activities in a time- and concentration-dependent manner with second-order...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2117

    authors： Xian M,Wang K,Chen X,Hou Y,McGill A,Zhou B,Zhang ZY,Cheng JP,Wang PG

    更新日期：2000-02-16 00:00:00

  • The role of active site residues in the oxidant specificity of the Orp1 thiol peroxidase.

    abstract：:In this study we investigated the role of active site residues in the peroxidase activity of Orp1 (GPx3) using three different peroxide substrates. Using a structural homology model of the reduced form of Orp1, we identified Asn126 and Phe127 as evolutionarily conserved residues that line the back of the Orp1 active s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.10.109

    authors： Takanishi CL,Ma LH,Wood MJ

    更新日期：2010-12-03 00:00:00

  • Multiple mechanisms of iron-induced ferritin synthesis in HeLa cells.

    abstract：:Iron administration to HeLa cells stimulates the accumulation of H-subunit and L-subunit rich isoferritins at similar extent. The increase in both types of isoferritins is accompanied by an increase in the amount of messenger RNAs specific for H and L subunits. The increase in the amount of these messenger RNAs, which...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91877-7

    authors： Cairo G,Bardella L,Schiaffonati L,Arosio P,Levi S,Bernelli-Zazzera A

    更新日期：1985-11-27 00:00:00

  • UDP-glucuronosyltransferase 1A7 polymorphisms are associated with liver cirrhosis.

    abstract：:Variations in the UDP-glucuronosyltransferase (UGT) 1A7 gene have been found to be related to the development of hepatocellular carcinoma (HCC). Since the pathogenesis of liver cirrhosis is not dissimilar to that of HCC, we hypothesized that UGT1A7 genetic polymorphisms may be associated with liver cirrhosis. PCR-rest...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.11.125

    authors： Tang KS,Lee CM,Teng HC,Huang MJ,Huang CS

    更新日期：2008-02-15 00:00:00

  • Role of apoptosis in growth and differentiation of proximal tubule cells in primary cultures.

    abstract：:In this study, we assessed the profile of cell proliferation, Na,K-ATPase activity and apoptosis in primary cultures of proximal tubule cells. Freshly isolated proximal tubules exhibited apoptosis very early in culture: floated cells within 6 hr; attached tubule cells within 20 hr. The onset of apoptosis in either att...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0118

    authors： Tang MJ,Cheng YR,Lin HH

    更新日期：1996-01-26 00:00:00

  • Loss of 3-chlorotyrosine by inflammatory oxidants: implications for the use of 3-chlorotyrosine as a bio-marker in vivo.

    abstract：:Activated neutrophils generate the potent oxidant hypochlorous acid (HOCl) from the enzyme myeloperoxidase (MPO). A proposed bio-marker for MPO-derived HOCl in vivo is 3-chlorotyrosine, elevated levels of which have been measured in several human inflammatory pathologies. However, it is unlikely that HOCl is produced ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.03.153

    authors： Whiteman M,Spencer JP

    更新日期：2008-06-20 00:00:00

  • Reversion of 7-methylguanosine 5'-phosphate inhibition of mRNA translation by polysomal and soluble factors isolated from Saccharomyces cerevisiae.

    abstract：:Protein fractions that overcome m7GMP inhibition of mRNA translation have been purified from the yeast S. cerevisiae. An active fraction isolated from polysomes contains two polypeptides of 220- and 190-kDa. The active fraction isolated from postribosomal supernatant contains a major polypeptide of 28-kDa and other sp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90760-1

    authors： Parets Suler A,Zueco J,Gozalbo D,Sentandreu R

    更新日期：1987-08-14 00:00:00

  • GEFH1 binds ASAP1 and regulates podosome formation.

    abstract：:Invadopodia are cellular structures that are thought to mediate tumor invasion. ASAP1, an Arf GTPase-activating protein (GAP) containing a BAR domain, is a substrate of Src. ASAP1 is required for the assembly of invadopodia and podosomes, which are Src-induced structures related to invadopodia in NIH 3T3 fibroblasts. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.02.093

    authors： Shiba Y,Randazzo PA

    更新日期：2011-03-25 00:00:00

  • Effects of YM264, a novel PAF antagonist, on puromycin aminonucleoside-induced nephropathy in the rat.

    abstract：:We investigated the effects of YM264, WEB2086, methylprednisolone and ticlopidine on puromycin-induced nephropathy in the rat. Puromycin produces marked proteinuria, hypercholesterolemia, and hypoalbuminemia. The structurally differing PAF antagonists YM264 and WEB2086 inhibited proteinuria and improved hypercholester...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80253-0

    authors： Yamada T,Tomioka K,Horie M,Sakurai Y,Nagaoka H,Mase T

    更新日期：1991-04-30 00:00:00

  • Two lutein molecules in LHCII have different conformations and functions: Insights into the molecular mechanism of thermal dissipation in plants.

    abstract：:When LHCII forms aggregates, the internal conformational changes will result in chlorophyll fluorescence quenching. Uncovering the molecular mechanism of this phenomenon will help us to understand how plants dissipate the excess excitation energy through non-photochemical quenching (NPQ) process. The crystal structure...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.01.172

    authors： Yan H,Zhang P,Wang C,Liu Z,Chang W

    更新日期：2007-04-06 00:00:00

  • Hydrogen-rich medium protects human skin fibroblasts from high glucose or mannitol induced oxidative damage.

    abstract：:Reactive oxygen species (ROS) are an important factor in the development of skin lesions in diabetes. A new antioxidant, hydrogen, can selectively neutralize hydroxyl radicals (()OH) and peroxynitrite (ONOO(-)) in cell-free systems, whereas it seldom reacts with other ROS. Fibroblasts are a key component of skin. In t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.05.024

    authors： Yu P,Wang Z,Sun X,Chen X,Zeng S,Chen L,Li S

    更新日期：2011-06-03 00:00:00

  • Binding of anti-acetylcholine receptor antibodies inhibits the acetylcholine receptor mediated cation flux.

    abstract：:A fast kinetics, spectroscopic technique has been applied to the study of the transient cation flux associated to the binding of cholinergic agonist to native acetylcholine receptor (AcChR)-rich membrane vesicles in presence of anti-AcChR antibodies. The technique is based on the collisional quenching of an intravesic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91263-4

    authors： Gonzalez-Ros JM,Ferragut JA,Martinez-Carrion M

    更新日期：1984-04-30 00:00:00

  • Conserved motifs in voltage-sensing and pore-forming modules of voltage-gated ion channel proteins.

    abstract：:Voltage-gated ion channels (VGCs) mediate selective diffusion of ions across cell membranes to enable many vital cellular processes. Three-dimensional structure data are lacking for VGC proteins; hence, to better understand their function, there is a need to identify the conserved motifs using sequence analysis method...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.190

    authors： Guda P,Bourne PE,Guda C

    更新日期：2007-01-12 00:00:00

  • Effects of tributyltin on barrier functions in human intestinal Caco-2 cells.

    abstract：:The effect of tributyltin (TBT) on human intestinal epithelial cell functions was investigated by using human intestinal Caco-2 cell monolayers. We paid particular attention to the effect of TBT on two barrier functions: the tight junction as a physical barrier and MDR1/P-glycoprotein as a biological barrier. A loss o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.01.147

    authors： Tsukazaki M,Satsu H,Mori A,Sugita-Konishi Y,Shimizu M

    更新日期：2004-03-19 00:00:00

  • Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics.

    abstract：:Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rap...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.04.070

    authors： Bamba Y,Shofuda T,Kanematsu D,Nonaka M,Yamasaki M,Okano H,Kanemura Y

    更新日期：2014-05-16 00:00:00

  • Glucose effect on the expression of 150 kDa oxygen-regulated protein in HeLa cells.

    abstract：:Chaperones assist in the correct folding of newly synthesised proteins in the endoplasmic reticulum (ER) of cells, this being essential for the translocation of protein molecules to specific subcellular compartments, extracellular matrix or to biological fluids. The biosynthesis of some ER chaperones is regulated by g...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.09.139

    authors： Cechowska-Pasko M,Bańkowski E,Chene P

    更新日期：2005-11-25 00:00:00

  • Functional characterization of T7 and T8 of human apolipoprotein (a).

    abstract：:Lipoprotein (a) [Lp(a)], a risk factor for coronary artery disease, is a LDL-like particle with apolipoprotein (a) [apo(a)] covalently linked to apolipoprotein B (apoB). Apo(a) has many repeats of kringle 4-like domain, classified as type 1 through type 10 (T1-T10). Deletion analysis was performed to define the functi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9478

    authors： Trieu VN,McConathy WJ

    更新日期：1998-10-09 00:00:00

  • Hypothermia followed by rapid rewarming exacerbates ischemia-induced brain injury and augments inflammatory response in rats.

    abstract：:Hypothermia followed by slow rewarming is neuroprotective for ischemic stroke. However, slow rewarming causes patients' longer stay in intensive care unit and increases the risk of hypothermic complications. Hypothermia followed by rapid rewarming (HTRR) is more convenient; but it exacerbates intracranial hypertension...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.04.095

    authors： Zhu SZ,Gu Y,Wu Z,Hu YF,Pan SY

    更新日期：2016-05-20 00:00:00

  • Comparison of the ischemic and non-ischemic lung cancer metabolome reveals hyper activity of the TCA cycle and autophagy.

    abstract：:Recent advances in cancer biology reveal the importance of metabolic changes in cancer; however, less is known about how metabolic pathways in tumors are regulated in vivo. Here, we report analysis of the lung cancer metabolism based on different surgical procedures, namely lobectomy and partial resection. In lobectom...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.07.082

    authors： Kikuchi N,Soga T,Nomura M,Sato T,Sakamoto Y,Tanaka R,Abe J,Morita M,Shima H,Okada Y,Tanuma N

    更新日期：2020-09-10 00:00:00

  • Src supports UDP-glucuronosyltransferase-2B7 detoxification of catechol estrogens associated with breast cancer.

    abstract：:Mammary gland-distributed and ER-bound UDP-glucuronosyltransferase (UGT)-2B7 metabolizes genotoxic catechol-estrogens (CE) associated with breast cancer initiation. Although UGT2B7 has 3 PKC- and 2 tyrosine kinase (TK)-sites, its inhibition by genistein, herbimycin-A and PP2 with parallel losses in phospho-tyrosine an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.03.054

    authors： Mitra PS,Basu NK,Owens IS

    更新日期：2009-05-15 00:00:00

  • Cell cycle-related signaling pathways modulated by peripheral benzodiazepine receptor ligands in colorectal cancer cells.

    abstract：:Specific ligands of the peripheral benzodiazepine receptor (PBR) have been shown to induce both apoptosis and G1/G0 cell cycle arrest in colorectal cancers. The signaling pathways leading to cell cycle arrest are still unknown. Using cDNA array technology, we identified signaling molecules involved in cell cycle arres...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.127

    authors： Maaser K,Sutter AP,Krahn A,Höpfner M,Grabowski P,Scherübl H

    更新日期：2004-11-12 00:00:00

  • Stabilizing effect of lysosomal beta-galactosidase on the catalytic activity of protective protein/cathepsin A secreted by human platelets.

    abstract：:The 32/20-kDa two-chain form of protective protein/cathepsin A (CathA) secreted by human platelets was thermostable in the aggregation supernatant at acidic pH, but was denatured at neutral pH. Leupeptin partly protected the CathA against denaturation, which was not observed in the supernatant after depletion of the c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9696

    authors： Itoh K,Naganawa Y,Kamei S,Shimmoto M,Sakuraba H

    更新日期：1998-12-18 00:00:00

  • Interaction of small G protein signaling modulator 3 with connexin 43 contributes to myocardial infarction in rat hearts.

    abstract：:Connexin 43 (Cx43), a ubiquitous connexin expressed in the heart and skin, is associated with a variety of hereditary conditions. Therefore, the characterization of Cx43-interacting proteins and their dynamics is important to understand not only the molecular mechanisms underlying pathological malfunction of gap junct...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.07.081

    authors： Lee CY,Choi JW,Shin S,Lee J,Seo HH,Lim S,Lee S,Joo HC,Kim SW,Hwang KC

    更新日期：2017-09-16 00:00:00

  • KSHV RTA induces a transcriptional repressor, HEY1 that represses rta promoter.

    abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) is a gamma-herpesvirus that is frequently associated with the formation of tumors like Kaposi's sarcoma and primary effusion lymphomas. KSHV RTA is required for lytic replication/reactivation in KSHV-infected cells. We developed an inducible RTA expression cell line to se...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.04.092

    authors： Yada K,Do E,Sakakibara S,Ohsaki E,Ito E,Watanabe S,Ueda K

    更新日期：2006-06-23 00:00:00

  • A mechanism for propagated SOD1 misfolding from frustration analysis of a G85R mutant protein assembly.

    abstract：:Application of landscape theory and the dehydron hypothesis to a crystal structure of a G85R mutant superoxide dismutase (SOD1) tetrameric complex allows for the description of a prion-like hypothesis that serves to explain propagated SOD1 misfolding. We have developed two conformational-change scenarios, one local to...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.08.172

    authors： Healy EF

    更新日期：2016-09-30 00:00:00

  • MtHsp70-CLIC1-pulsed dendritic cells enhance the immune response against ovarian cancer.

    abstract：:Approximately 80% of ovarian cancer (OC) is diagnosed at late stages, and most patients die within 5 years of diagnosis due to recurrence or drug resistance. Novel treatments are required to improve patient survival. Immune therapy against cancer is promising; however, therapeutic vaccination has been limited by the i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.10.094

    authors： Yu W,Qu H,Cao G,Liu C,Deng H,Zhang Z

    更新日期：2017-12-09 00:00:00

  • Rescue of ligand binding of a mutant IGF-I receptor by complementation.

    abstract：:The IGF-I receptor binds IGF-I with complex kinetics characterized by a curvilinear Scatchard plot, suggesting receptor heterogeneity and apparent negative cooperativity. To explore the molecular mechanisms underlying these properties, we have characterized the binding of a hybrid receptor formed from a wild-type rece...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.122

    authors： Chakravarty A,Hinrichsen J,Whittaker L,Whittaker J

    更新日期：2005-05-27 00:00:00