当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Hydrogen-rich medium protects human skin fibroblasts from high glucose or mannitol induced oxidative damage.

Hydrogen-rich medium protects human skin fibroblasts from high glucose or mannitol induced oxidative damage.

Abstract：

:Reactive oxygen species (ROS) are an important factor in the development of skin lesions in diabetes. A new antioxidant, hydrogen, can selectively neutralize hydroxyl radicals (()OH) and peroxynitrite (ONOO(-)) in cell-free systems, whereas it seldom reacts with other ROS. Fibroblasts are a key component of skin. In the present study, we investigated the protective effects of hydrogen-rich medium on human skin fibroblasts (HSFs) under oxidative stress. Confocal microscopy was used to assay both the intracellular superoxide anion (O(2)(-)) concentration and the mitochondrial membrane potential (ΔΨ). Cell viability was determined using the Cell Counting Kit-8 (CCK-8). The concentrations of cellular malonaldehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 3-nitrotyrosine (3-NT) were also measured. The results revealed that both mannitol and high glucose could cause oxidative stress in HSFs. Interestingly, the use of a hydrogen-rich medium significantly reduced the level of intracellular O(2)(-), stabilized the ΔΨ and attenuated production of MDA, 8-OHdG and 3-NT which efficiently enhanced the antioxidative defense system and protected the HSFs from subsequent oxidative stress damage. In other words, hydrogen decreased the excessive generation of intracellular O(2)(-) and elevated the cellular antioxidative defense. Based on our results, hydrogen may have applications in the treatment of skin diseases caused by diabetes.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Yu P,Wang Z,Sun X,Chen X,Zeng S,Chen L,Li S

doi

10.1016/j.bbrc.2011.05.024

subject

Has Abstract

pub_date

2011-06-03 00:00:00

pages

350-5

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(11)00777-7

journal_volume

409

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Glucagonlike peptide-1(7-36)amide suppresses glucagon secretion and decreases cyclic AMP concentration in cultured In-R1-G9 cells.

    abstract：:We previously reported that GLP-1(7-36)amide had glucagonostatic action as well as insulinotropic action in the perfused rat pancreas. In this study, we examined the effect of GLP-1(7-36)amide on glucagon secretion and cAMP concentration in glucagon-secreting cell line, In-R1-G9. GLP-1(7-36)amide (1nM) significantly s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80836-8

    authors： Matsumura T,Itoh H,Watanabe N,Oda Y,Tanaka M,Namba M,Kono N,Matsuyama T,Komatsu R,Matsuzawa Y

    更新日期：1992-07-15 00:00:00

  • The effects of curcumin, mangiferin, resveratrol and other natural plant products on aminopeptidase B activity.

    abstract：:Aminopeptidase B (Ap-B) is a Zn2+-aminopeptidase of the M1 family which is implicated, in conjunction with the nardilysin endoprotease, in the generation of miniglucagon, a peptide involved in the maintenance of glucose homeostasis. Other in vivo physiological roles have been established for this vertebrate enzyme, su...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.02.143

    authors： Cadel S,Darmon C,Désert A,Mahbouli M,Piesse C,Ghélis T,Lafont R,Foulon T

    更新日期：2019-05-14 00:00:00

  • Crystal structure of geranylgeranyl pyrophosphate synthase (crtE) from Nonlabens dokdonensis DSW-6.

    abstract：:Isoprenoids comprise a diverse group of natural products with a broad range of metabolic functions. Isoprenoids are synthesized from prenyl pyrophosphates by prenyltransferases that catalyze the isoprenoid chain-elongation process to different chain lengths. We hereby present the crystal structure of geranylgeranyl py...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.08.071

    authors： Kim S,Kim EJ,Park JB,Kim SW,Kim KJ

    更新日期：2019-10-20 00:00:00

  • Hydrogen from intestinal bacteria is protective for Concanavalin A-induced hepatitis.

    abstract：:It is well known that some intestinal bacteria, such as Escherichia coli, can produce a remarkable amount of molecular hydrogen (H(2)). Although the antioxidant effects of H(2) are well documented, the present study examined whether H(2) released from intestinally colonized bacteria could affect Concanavalin A (ConA)-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.06.024

    authors： Kajiya M,Sato K,Silva MJ,Ouhara K,Do PM,Shanmugam KT,Kawai T

    更新日期：2009-08-21 00:00:00

  • The juxtamembrane domain in ETV6/FLT3 is critical for PIM-1 up-regulation and cell proliferation.

    abstract：:We recently reported that the ETV6/FLT3 fusion protein conferred interleukin-3-independent growth on Ba/F3 cells. The present study has been conducted to assess role of the juxtamembrane domain of FLT3 for signal transduction and cell transformation. The wild-type ETV6/FLT3 fusion protein in transfected cells was a co...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.03.157

    authors： Vu HA,Xinh PT,Kano Y,Tokunaga K,Sato Y

    更新日期：2009-06-05 00:00:00

  • Alpha-agglutinin expression in Saccharomyces cerevisiae.

    abstract：:A polyclonal antiserum raised against purified alpha-agglutinin was made specific for alpha-agglutinin after adsorption with a cells. The adsorbed antiserum identified alpha-agglutinin peptides on Western blots and bound to cell surface alpha-agglutinin, inhibiting the binding of alpha cells to a cells. Using the anti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91557-x

    authors： Wojciechowicz D,Lipke PN

    更新日期：1989-05-30 00:00:00

  • PhyA gene product of Aspergillus ficuum and Peniophora lycii produces dissimilar phytases.

    abstract：:PhyA gene products of Aspergillus ficuum (AF) and Peniophora lycii (PL) as expressed in industrial strains of Aspergillus niger and Aspergillus oryzae, respectively, were purified to homogeneity and then characterized for both physical and biochemical properties. The PL phytase is 26 amino acid residues shorter than t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00374-7

    authors： Ullah AH,Sethumadhavan K

    更新日期：2003-04-04 00:00:00

  • Formation of monohydroxyeicosatetraenoic acids from arachidonic acid by cultured rabbit aortic smooth muscle cells.

    abstract：:In addition to the well established cyclooxygenase pathway, cultured aortic smooth muscle cells convert arachidonic acid to several polar metabolites identified by high performance liquid chromatography and gaz chromatography-mass spectrometry. 15-Hydroxyeicosatetraenoic acid, 12-Hydroxyeicosatetraenoic acid and 5-Hyd...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91822-3

    authors： Larrue J,Rigaud M,Razaka G,Daret D,Demond-Henri J,Bricaud H

    更新日期：1983-04-15 00:00:00

  • Tissue-specific and dexamethasone-inducible expression of alkaline phosphatase from alternative promoters of the rat bone/liver/kidney/placenta gene.

    abstract：:The rat Bone/Liver/kidney/Placenta Alkaline Phosphatase (ALP) is transcribed from two alternative promoters spaced over 25 kb apart, resulting in two variant transcripts that are identical in their coding sequence. We investigated the steady-state levels of the two variant transcripts in various rat tissues and cell l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90405-v

    authors： Zernik J,Kream B,Twarog K

    更新日期：1991-05-15 00:00:00

  • Trypanosoma cruzi: TcRAB7 protein is localized at the Golgi apparatus in epimastigotes.

    abstract：:In mammalian cells, the Rab7 protein is a key element of late endocytic membrane traffic. Several results suggest that it is involved in the transport from early to late endosome or from late endosome to lysosome. We have previously characterized a Rab7 gene homologue (TcRAB7) in Trypanosoma cruzi. Now, using an affin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.159

    authors： Araripe JR,Cunha e Silva NL,Leal ST,de Souza W,Rondinelli E

    更新日期：2004-08-20 00:00:00

  • A missense mutation in the RING finger motif of PEX2 protein disturbs the import of peroxisome targeting signal 1 (PTS1)-containing protein but not the PTS2-containing protein.

    abstract：:SK24 and PT54 mutant cells, which are peroxisome-deficient Chinese hamster ovary (CHO) cells isolated using peroxisomal forms of green fluorescent protein (GFP), were found to be defective in the PEX2 gene. The nucleotide sequences of PEX2 cDNA from the mutant cells were determined to identify mutation sites in the mu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2510

    authors： Huang Y,Ito R,Miura S,Hashimoto T,Ito M

    更新日期：2000-04-21 00:00:00

  • Crystal structure and biophysical characterisation of Helicobacter pylori phosphopantetheine adenylyltransferase.

    abstract：:Helicobacter pylori is a bacterium that causes chronic active gastritis and peptic ulcers. Drugs targeting H. pylori phosphopantetheine adenylyltransferase (HpPPAT), which is involved in CoA biosynthesis, may be useful. Herein, we report the expression in Escherichia coli and purification of recombinant HpPPAT and des...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.04.058

    authors： Cheng CS,Chen CH,Luo YC,Chen WT,Chang SY,Lyu PC,Kao MC,Yin HS

    更新日期：2011-05-06 00:00:00

  • Calcium effects on prothrombin and its reaction with bifunctional alkylating reagents.

    abstract：:Monodisperse bovine prothrombin was prepared and its molecular states under several conditions examined. The protein showed no tendency to self-associate in the absence of calcium. Calcium (4 mM) caused small increases in the apparent molecular weight of the protein which may or may not represent protein dimerization ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(83)80138-7

    authors： Silversmith RE,Wei GJ,Nelsestuen GL

    更新日期：1983-02-28 00:00:00

  • Lithium prevents early cytosolic calcium increase and secondary injurious calcium overload in glycolytically inhibited endothelial cells.

    abstract：:Cytosolic free calcium concentration ([Ca(2+)]i) is a central signalling element for the maintenance of endothelial barrier function. Under physiological conditions, it is controlled within narrow limits. Metabolic inhibition during ischemia/reperfusion, however, induces [Ca(2+)]i overload, which results in barrier fa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.03.047

    authors： Bosche B,Schäfer M,Graf R,Härtel FV,Schäfer U,Noll T

    更新日期：2013-05-03 00:00:00

  • Pilot study to determine the feasibility of producing protease-resistant prion protein fragments by random PCR mutagenesis.

    abstract：:We report the results of a pilot study to determine the feasibility of using PCR random mutagenesis and in vitro transcription/translation to produce protease resistant full-length or truncated ovine prion proteins (PrP). Using this approach, we show the novel production of protease resistant recombinant ovine prion p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4450

    authors： Dear DV,Fitzmaurice TJ,Garton S,Richards SJ

    更新日期：2001-03-09 00:00:00

  • Molecular mechanisms for Kv1.3 potassium channel current inhibition by CD3/CD28 stimulation in Jurkat T cells.

    abstract：:In T lymphocyte, activation of Kv1.3 channel, the major voltage-dependent K(+) channel, is an essential step for cell proliferation in immune responses. Here, effects of anti-CD3 and anti-CD28 antibodies on Kv1.3 current were examined in three types of human T lymphocyte derived cell lines, Jurkat E6-1, p56lck-kinase ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.06.118

    authors： Matsushita Y,Ohya S,Itoda H,Kimura T,Suzuki Y,Yamamura H,Imaizumi Y

    更新日期：2008-09-12 00:00:00

  • Covalent circularization of exogenous RNA during incubation with a wheat embryo cell extract.

    abstract：:Cell extracts from wheat embryos have been widely used for mRNA-directed protein production. Here, we found that a significant fraction of exogenous linear RNAs are circularized in a wheat embryo extract. The circularization was seen only in uncapped RNAs. The amount of the circular species reached around 1% of the in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.07.011

    authors： Makino S,Sawasaki T,Tozawa Y,Endo Y,Takai K

    更新日期：2006-09-08 00:00:00

  • MicroRNA-187, down-regulated in clear cell renal cell carcinoma and associated with lower survival, inhibits cell growth and migration though targeting B7-H3.

    abstract：:Aberrantly expressed microRNAs (miRNAs) are frequently associated with the aggressive malignant behavior of human cancers, including clear cell renal cell carcinoma (ccRCC). Based on the preliminary deep sequencing data, we hypothesized that miR-187 may play an important role in ccRCC development. In this study, we fo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.07.095

    authors： Zhao J,Lei T,Xu C,Li H,Ma W,Yang Y,Fan S,Liu Y

    更新日期：2013-08-23 00:00:00

  • Calmodulin mutant in central linker reduces the binding affinity with PreIQ and IQ while interacting with CaV1.2 channels.

    abstract：:Calmodulin (CaM) was reported to interact with PreIQ and IQ of CaV1.2 channels, but to date, no explicit binding sites of CaM were illustrated. Therefore, in the present study, we firstly used MOE (Molecular Operating Environment) for protein-protein docking and we found that the most likely residues of CaM that play ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.03.018

    authors： Liu Y,Yan S,Chen S,Zhang J,Shen Y,Su J,He G,Feng R,Shao D,Hao L

    更新日期：2020-05-21 00:00:00

  • Valine, the branched-chain amino acid, suppresses hepatitis C virus RNA replication but promotes infectious particle formation.

    abstract：BACKGROUND & AIMS:Concentrations of the branched-chain amino acid (BCAA) in the serum of patients with liver cirrhosis correlate with their liver function. Oral administration of BCAA can ameliorate hypoalbuminemia and hepatic encephalopathy. In this study, we aim to clarify the role of BCAA in regulating the replicati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.06.051

    authors： Ishida H,Kato T,Takehana K,Tatsumi T,Hosui A,Nawa T,Kodama T,Shimizu S,Hikita H,Hiramatsu N,Kanto T,Hayashi N,Takehara T

    更新日期：2013-07-19 00:00:00

  • Dynamics of oxygen level-driven regulators in modulating autophagy in colorectal cancer cells.

    abstract：:Colorectal cancer is a common cancer with metachronous distant metastases still threatening overall survival. Tumor oxygen level influences tumor radiosensitivity in relation to autophagy and apoptosis. The objective of this study is to evaluate the expression and interaction between multiple key regulators in differe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.07.043

    authors： Tam SY,Wu VWC,Law HKW

    更新日期：2019-09-17 00:00:00

  • Unexpected relationships between bacteriophage lambda hypothetical proteins and bacteriophage T4 tail-fiber proteins.

    abstract：:Hypothetical lambda protein ORF314 shows significant homology with the carboxyl end of phage T4 tail-fiber protein gp37. Homology can also be demonstrated between hypothetical lambda protein ORF194 and a fragment of bacteriophage T4 protein gp38. This sequence homology is also reflected in the genomic sequences of the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(83)80043-6

    authors： George DG,Yeh LS,Barker WC

    更新日期：1983-09-30 00:00:00

  • Promyelocytic leukemia cell line, HL-60, produces human hepatocyte growth factor.

    abstract：:The human promyelocytic leukemia cell line, HL-60, stimulated with PMA, produced human HGF-like immunoreactivity (HL-60 HGF), which was detected with human HGF-specific ELISA. The purified HL-60 HGF was indistinguishable from human HGF in the plasma of patients with fulminant hepatic failure by studies of subunit cons...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81421-4

    authors： Nishino T,Kaise N,Sindo Y,Nishino N,Nishida T,Yasuda S,Masui Y

    更新日期：1991-11-27 00:00:00

  • Drosophila melanogaster larval hemolymph protein mapping.

    abstract：:With the completion of the genome sequence of Drosophila melanogaster the importance of constructing a proteome map is to be considered. Therefore, with the application of recent advances in proteomic analysis approaches, a protein map of D. melanogaster larvae hemolymph proteins was obtained using 2-DE in the range o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.10.156

    authors： Guedes Sde M,Vitorino R,Tomer K,Domingues MR,Correia AJ,Amado F,Domingues P

    更新日期：2003-12-19 00:00:00

  • P202, an interferon-inducible protein, inhibits E2F1-mediated apoptosis in prostate cancer cells.

    abstract：:p202, an interferon (IFN) inducible protein, is a phosphonuclear protein involved in the regulation of cell cycle, apoptosis, and differentiation. E2F1 belongs to the E2F family of proteins that are important cell cycle regulators in promoting cell growth. On the other hand, the deregulated expression of E2F1 also tri...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00320-6

    authors： Yan DH,Abramian A,Li Z,Ding Y,Wen Y,Liu TJ,Hunt K

    更新日期：2003-03-28 00:00:00

  • Lactation suppresses diurnal rhythm of serum leptin.

    abstract：:Rats consume most of their daily food intake at night; serum leptin levels and adipose tissue leptin mRNA content are elevated at night in non-lactating rats fed ad libitum. Lactation induces massive hyperphagia with most food still consumed at night, but the nocturnal increase in leptin secretion was not observed in ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8934

    authors： Pickavance L,Tadayyon M,Williams G,Vernon RG

    更新日期：1998-07-09 00:00:00

  • Affinity selection to papain yields potent peptide inhibitors of cathepsins L, B, H, and K.

    abstract：:Endogenous cysteine proteases were given much attention lately, as their role in a variety of pathophysiological disorders became evident. Amongst them cathepsins, which are thought to be implicated in mediation of osteoporosis, cancer progression, atherosclerosis, and many other conditions, are of considerable intere...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.05.028

    authors： Bratkovic T,Lunder M,Popovic T,Kreft S,Turk B,Strukelj B,Urleb U

    更新日期：2005-07-08 00:00:00

  • Self-association of the hepatitis B virus X protein in the yeast two-hybrid system.

    abstract：:Self-association of the transactivator HBx protein of hepatitis B virus was investigated using the yeast two-hybrid system. Expression vectors for the full-length HBx (X0) and its truncated mutants (X15 and X16) were constructed by separately ligating the DNA-binding (BD) and transactivation domains (AD) of Gal4. Co-t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.140

    authors： Reddi HV,Kumar V

    更新日期：2004-05-14 00:00:00

  • Vaspin protects vascular endothelial cells against free fatty acid-induced apoptosis through a phosphatidylinositol 3-kinase/Akt pathway.

    abstract：:Vaspin, an adipocytokine recently identified in a rat model of type 2 diabetes, has been suggested to have an insulin-sensitizing effect. However, the exact mechanism underlying this action has not been fully elucidated. Furthermore, the specific function of vaspin is largely unknown, especially in vascular cells. We ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.08.083

    authors： Jung CH,Lee WJ,Hwang JY,Seol SM,Kim YM,Lee YL,Park JY

    更新日期：2011-09-23 00:00:00

  • The down-regulation of cardiac contractile proteins underlies myocardial depression during sepsis and is mitigated by carbon monoxide.

    abstract：:The aim of this study is to investigate the mechanism underling cardiac dysfunction during sepsis, as well as the possible amelioration of this dysfunction by exogenous carbon monoxide (CO) administration. For this purpose, rats (six-week-old, male, Sprague-Dawley) were administered LPS (15 mg/kg body weight, i.p. 6 h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.12.020

    authors： Unuma K,Aki T,Nagano S,Watanabe R,Uemura K

    更新日期：2018-01-08 00:00:00