当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Crystal structure of geranylgeranyl pyrophosphate synthase (crtE) from Nonlabens dokdonensis DSW-6.

Crystal structure of geranylgeranyl pyrophosphate synthase (crtE) from Nonlabens dokdonensis DSW-6.

Abstract：

:Isoprenoids comprise a diverse group of natural products with a broad range of metabolic functions. Isoprenoids are synthesized from prenyl pyrophosphates by prenyltransferases that catalyze the isoprenoid chain-elongation process to different chain lengths. We hereby present the crystal structure of geranylgeranyl pyrophosphate synthase from the marine flavobacterium Nonlabens dokdonensis DSW-6 (NdGGPPS). NdGGPPS forms a hexamer composed of homodimeric trimer, and the monomeric structure is composed of 15 α-helices (α1-α15). In this structure, we observed the binding of one pyrophosphate molecule and two glycerol molecules that mimicked substrate binding to the enzyme. The substrate binding site of NdGGPPS contains large hydrophobic residues such as Phe, His and Tyr, and structural and amino acids sequence analyses thereof suggest that the protein belongs to the short-chain prenyltransferase family.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kim S,Kim EJ,Park JB,Kim SW,Kim KJ

doi

10.1016/j.bbrc.2019.08.071

subject

Has Abstract

pub_date

2019-10-20 00:00:00

pages

479-485

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(19)31588-8

journal_volume

518

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • CPuORF correlates with miRNA responsive elements on protein evolutionary rates.

    abstract：:miRNA is increasingly being recognized as a key regulator of metabolism in animals. A wealth of evidence has suggested that miRNA mainly binds 3' UTR of mRNA and modulates the cell activities via repressing the mRNA translation. However, as the translation initiates at 5' UTR, cis elements like upstream open reading f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.08.050

    authors： Hu W,Wang T,Yang Y,Zheng S

    更新日期：2014-09-12 00:00:00

  • Ligand effects on the limited proteolysis of phenylalanine hydroxylase: evidence for multiple conformational states.

    abstract：:The effects of phenylalanine and tetrahydrobiopterin on the limited proteolysis of rat liver phenylalanine hydroxylase by chymotrypsin have been examined. The presence of tetrahydrobiopterin inhibits the proteolytic activation of native phenylalanine hydroxylase. In contrast, phenylalanine causes a stimulation of prot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91050-1

    authors： Phillips RS,Iwaki M,Kaufman S

    更新日期：1983-02-10 00:00:00

  • KMT Set7/9 is a new regulator of Sam68 STAR-protein.

    abstract：:Lysine-specific methyltransferase Set7/9 (KMT7) belongs to the SET domain family of proteins. Besides the SET domain, Set7/9 also contains a so-called MORN (Membrane Occupation and Recognition Nexus) domain whose function in high eukaryotes is largely unknown. Set7/9 has been shown to specifically methylate both histo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.03.017

    authors： Vasileva E,Shuvalov O,Petukhov A,Fedorova O,Daks A,Nader R,Barlev N

    更新日期：2020-05-14 00:00:00

  • Detection of Choristoneura fumiferana brush border membrane-binding molecules specific to Bacillus thuringiensis delta-endotoxin by crossed affinity immunoelectrophoresis.

    abstract：:Brush border membrane vesicles (BBMVs) prepared from midguts of Choristoneura fumiferana larvae were pretreated separately with the three CryIA delta-endotoxins from Bacillus thuringiensis subsp. kurstaki HD-1. Crossed affinity immunoelectrophoresis of the solubilized toxin-BBMV complexes, using antibodies raised agai...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1357

    authors： Pang AS

    更新日期：1994-03-30 00:00:00

  • Characterization of virtual coupling in the proton nuclear magnetic resonance spectrum of N,N'-diacetylchitobiose by two-dimensional J-resolved spectroscopy.

    abstract：:The anomeric proton of the reducing N-acetylglucosamine residue of beta-N,N'-diacetylchitobiose exhibited an unusual lineshape indicative of virtual coupling in the 250 MHz proton NMR spectrum. The two-dimensional J-resolved spectrum contained four lines associated with this proton, and this pattern can be used to dis...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90265-1

    authors： Bruch RC,Bruch MD,Noggle JH,White HB 3rd

    更新日期：1984-09-17 00:00:00

  • Heterogeneity of insulin-like growth factor-I affinity for the insulin-like growth factor-II receptor: comparison of natural, synthetic and recombinant DNA-derived insulin-like growth factor-I.

    abstract：:Although insulin-like growth factors (IGF) I and II bind with high affinity to structurally discrete receptors, they bind with a lesser affinity to each other's receptor. We have evaluated the affinity of five different IGF-I preparations (three natural IGF-I preparations, one synthetic preparation, and one recombinan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90650-4

    authors： Rosenfeld RG,Conover CA,Hodges D,Lee PD,Misra P,Hintz RL,Li CH

    更新日期：1987-02-27 00:00:00

  • Selective inhibition of human immunodeficiency virus (HIV) by 3'-azido-2', 3'-dideoxyguanosine in vitro.

    abstract：:3'-Azido-2',3'-dideoxyguanosine (AzddGuo) is a potent and selective inhibitor of human immunodeficiency virus (HIV) in vitro. AzddGuo completely inhibits HIV-induced cytopathogenicity and viral antigen expression in MT-4 cells at a concentration of 5.0 microM. Its 50% effective dose for inhibiting HIV-induced cytopath...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91547-6

    authors： Baba M,Pauwels R,Balzarini J,Herdewijn P,De Clercq E

    更新日期：1987-06-30 00:00:00

  • Centaurin-alpha 1 associates in vitro and in vivo with nucleolin.

    abstract：:Centaurin-alpha(1) was originally described as a binding partner for phosphoinositides. In spite of the presence of a putative ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) domain, no ARF-GAP activity has been attributed to centaurin-alpha(1) so far. Thus the function of this protein remains to be dete...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)03010-3

    authors： Dubois T,Zemlickova E,Howell S,Aitken A

    更新日期：2003-02-07 00:00:00

  • Enzymic and nonenzymic mono ADP-ribosylation of proteins in skeletal muscle.

    abstract：:The acceptors of endogenously catalyzed monoADP-ribosylation in the cell free extract from rat skeletal muscle was searched. The main acceptor proteins in particulate were electrophoretically 52, 80, 100, and greater than 200 kDa proteins in the presence of SDS, while that in cytosol were 36 and 39 kDa proteins. Altho...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92329-2

    authors： Tanaka Y,Yoshihara K,Kamiya T

    更新日期：1989-09-15 00:00:00

  • Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells.

    abstract：:We previously showed in a primary culture of rat testicular cells that spermatogenic cells specifically bind to somatic Sertoli cells and that this interaction is needed for spermatogenic cells to differentiate in vitro. Adopting an expression cloning procedure, we here isolated a cDNA coding for a spermatogenic cell ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8918

    authors： Maeda T,Sakashita M,Ohba Y,Nakanishi Y

    更新日期：1998-07-09 00:00:00

  • Effect of retinoic acid on the acylation of phospholipids in granulocytes in vitro.

    abstract：:The influence of retinoic acid on the incorporation of [1-14C]palmitic acid and [1-14C]arachidonic acid into phospholipids was examined in guinea pig peritoneal granulocytes. All-trans-retinoic acid inhibited the incorporation of both fatty acids into phosphatidic acid and phosphatidylinositol. However, it stimulated ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91622-4

    authors： Tou J

    更新日期：1983-07-18 00:00:00

  • Structural properties of metal-free apometallothioneins.

    abstract：:The metalated forms of metallothionein are well studied (particularly Zn-MT, Cu-MT and Cd-MT), but almost nothing is known about the chemical and structural properties of apometallothioneins despite their importance in initial metalation and subsequent demetalation. Electrospray ionization mass spectrometry was used t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.07.141

    authors： Summers KL,Mahrok AK,Dryden MD,Stillman MJ

    更新日期：2012-08-24 00:00:00

  • Insulin receptors on Chinese hamster ovary (CHO) cells: altered insulin binding to glycosylation mutants.

    abstract：:We have identified and characterized insulin receptors on Chinese hamster ovary (CHO) cells. Insulin binds in a time, temperature and pH dependent fashion and insulin analogues compete for 125I-insulin binding in order of their biological potencies. Furthermore, two CHO cell glycosylation mutants, B4-2-1, lacking high...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80335-6

    authors： Podskalny J,McElduff A,Gorden P

    更新日期：1984-11-30 00:00:00

  • Cofactor activity of dihydroflavin mononucleotide and tetrahydrobiopterin for murine epididymal indoleamine 2,3-dioxygenase.

    abstract：:Dihydroflavin mononucleotide (FMNH2) and tetrahydrobiopterin (BH4) serve as cofactors for indoleamine 2,3-dioxygenase isolated from mouse epididymis. The optimal pH was between 7 and 8, and FMNH2-dependent activity was 4 to 5-fold higher than activity with methylene blue as the electron donor. Using FMNH2 with a FMN r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90339-6

    authors： Ozaki Y,Reinhard JF Jr,Nichol CA

    更新日期：1986-06-30 00:00:00

  • The natural product peiminine represses colorectal carcinoma tumor growth by inducing autophagic cell death.

    abstract：:Autophagy is evolutionarily conservative in eukaryotic cells that engulf cellular long-lived proteins and organelles, and it degrades the contents through fusion with lysosomes, via which the cell acquires recycled building blocks for the synthesis of new molecules. In this study, we revealed that peiminine induces ce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.04.102

    authors： Lyu Q,Tou F,Su H,Wu X,Chen X,Zheng Z

    更新日期：2015-06-19 00:00:00

  • Number and molecular brightness analysis reveals Htt25Q protein aggregation upon the uptake of Htt97Q aggregates.

    abstract：:Number and molecular Brightness (N&B) analysis is a powerful method used to monitor protein aggregation in living cells. Here, we used the N&B method to characterize the unexpanded HTT protein oligomerization after the internalization of the mutant HTT (mHTT) which contains a CAG repeat extensions encoding for long po...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.10.041

    authors： Sameni S,Zhang R,Digman MA

    更新日期：2020-01-29 00:00:00

  • Analysis of self-association of West Nile virus capsid protein and the crucial role played by Trp 69 in homodimerization.

    abstract：:The understanding of capsid (C) protein interactions with itself would provide important data on how the core is organized in flaviviruses during assembly. In this study, West Nile (WN) virus C protein was shown to form homodimers using yeast two-hybrid analysis in conjunction with mammalian two-hybrid and in vivo co-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.01.121

    authors： Bhuvanakantham R,Ng ML

    更新日期：2005-04-01 00:00:00

  • Identification of a binding site on Hsc70 for the immunosuppressant 15-deoxyspergualin.

    abstract：:Hsc70, the constitutive form of the heat shock protein 70 family of proteins, is involved in a number of biological activities which include protein folding and molecular chaperoning. Previously, we had shown that the immunosuppressant 15-deoxyspergualin (DSG) specifically interacted with Hsc70, as well as the Hsp90 f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9775

    authors： Nadler SG,Dischino DD,Malacko AR,Cleaveland JS,Fujihara SM,Marquardt H

    更新日期：1998-12-09 00:00:00

  • Mechanisms of sterol regulatory element-binding protein-2 (SREBP-2) regulation of human prostasin gene expression.

    abstract：:Prostasin is a glycosylphosphatidylinositol (GPI)-anchored serine protease and a suppressor of tumor cell invasion. We recently reported that the human prostasin gene is up-regulated by the transcription factor sterol regulatory element-binding protein-2 (SREBP-2). In the present study, we identified multiple SREBP-2 ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.06.049

    authors： Chen M,Chen LM,Chai KX

    更新日期：2006-08-11 00:00:00

  • Non-small cell lung cancer cells with deficiencies in homologous recombination genes are sensitive to PARP inhibitors.

    abstract：:Lung cancer is the leading cause of cancer death worldwide. PARP inhibitors have become a new line of cancer therapy and a successful demonstration of the synthetic lethality concept. The mechanism and efficacy of PARP inhibitors have been well studied in some cancers, especially homologous recombination (HR)-deficien...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.11.050

    authors： Ji W,Weng X,Xu D,Cai S,Lou H,Ding L

    更新日期：2020-01-29 00:00:00

  • Neurotensin-mediated activation of MAPK pathways and AP-1 binding in the human pancreatic cancer cell line, MIA PaCa-2.

    abstract：:Neurotensin (NT), a gastrointestinal (GI) hormone, binds its receptor (NTR) to stimulate proliferation of normal and neoplastic GI tissues; the molecular mechanisms remain largely undefined. Mitogen-activated protein kinases (MAPKs) are a family of intracellular kinases that transmit mitogenic signals by translocating...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2335

    authors： Ehlers RA,Zhang Y,Hellmich MR,Evers BM

    更新日期：2000-03-24 00:00:00

  • Urokinase binding sites on human foreskin cells. Evidence for occupancy with endogenous urokinase.

    abstract：:In cultures of human foreskin fibroblasts most of the cell surface binding sites for 2-chain urokinase are masked and can be exposed by 10 min. incubation on ice at pH 2.5 (A. Bajpai and J.B. Baker (1985), Biochem. Biophys. Res. Commun.133, 475-482). Here we show that incubation on ice at pH 2.5 also releases from the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91234-3

    authors： Bajpai A,Baker JB

    更新日期：1985-12-31 00:00:00

  • Synergistic regulation of the mouse orphan nuclear receptor SHP gene promoter by CLOCK-BMAL1 and LRH-1.

    abstract：:Small heterodimer partner (SHP; NR0B2) is an orphan nuclear receptor and acts as a repressor for wide variety of nuclear hormone receptors. We demonstrated here that mouse SHP mRNA showed a circadian expression pattern in the liver. Transient transfection of the mSHP promoter demonstrated that CLOCK-BMAL1, core circad...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.12.131

    authors： Oiwa A,Kakizawa T,Miyamoto T,Yamashita K,Jiang W,Takeda T,Suzuki S,Hashizume K

    更新日期：2007-02-23 00:00:00

  • ATP is a cryptic binder of TDP-43 RRM domains to enhance stability and inhibit ALS/AD-associated fibrillation.

    abstract：:ATP is the universal energy currency for all cells but has cellular concentrations of 2-12 mM, much higher than required for its classic functions. RNA-recognition motif (RRM) constitutes one of the most abundant domains in eukaryotes and most heterogeneous nuclear ribonucleoproteins (hnRNP) contain RRM domains which ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.11.088

    authors： Dang M,Kang J,Lim L,Li Y,Wang L,Song J

    更新日期：2020-01-29 00:00:00

  • Rundown of GH3 cell K+ conductance response to TRH following patch recording can be obviated with GH3 cell extract.

    abstract：:GH3/B6 pituitary cells release prolactin (PRL) in response to thyrotropin releasing hormone (TRH). Electrophysiological assays of individual GH3 cells with sharp high-resistance microelectrodes have revealed complex effects of TRH on membrane excitability consisting of a transient hyperpolarization (1), which is thoug...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91222-2

    authors： Dufy B,MacDermott A,Barker JL

    更新日期：1986-05-29 00:00:00

  • Expression of nitric oxide synthase in a murine model of viral myocarditis induced by coxsackievirus B3.

    abstract：:Nitric oxide is known to participate in the immune and inflammatory processes. In this study, we investigated the production of nitric oxide (NO) in murine viral myocarditis induced by coxsackievirus B3. The expression of inducible NO synthase (iNOS) mRNA in the heart first appeared on day 4 after virus inoculation an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0519

    authors： Mikami S,Kawashima S,Kanazawa K,Hirata K,Katayama Y,Hotta H,Hayashi Y,Ito H,Yokoyama M

    更新日期：1996-03-27 00:00:00

  • Fluorescence polarization study of lipids and membranes prepared from brain hemispheres of a hibernating mammal.

    abstract：:The physical behavior of total lipids, microsomes and microsomal lipids prepared from brain hemispheres of European Hamsters (Cricetus cricetus) was approached by the measure of the fluorescence polarization of the probe 1,6-diphenyl 1,3,5-hexatriene. We compare in this study the results obtained for two critical peri...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91665-6

    authors： Montaudon D,Robert J,Canguilhem B

    更新日期：1984-02-29 00:00:00

  • An inhibitory protein of intestinal fluid secretion reverses neuronal GABA transport.

    abstract：:Intestinal challenge with cholera toxin induces the synthesis of a hormone-like protein which counteracts intestinal hypersecretion. This study shows that the protein also inhibits GABA transport across the plasma membrane of Deiters' cells in rabbits. The inhibitory action of the protein was dose dependent, and 10(3)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91719-x

    authors： Lange S,Lönnroth I,Palm A,Hydén H

    更新日期：1985-08-15 00:00:00

  • Overexpression of BCL2 blocks TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in human lung cancer cells.

    abstract：:The tumor necrosis factor (TNF) related apoptosis-inducing ligand (TRAIL or Apo2L) and its receptors are members of the tumor necrosis factor superfamily. TRAIL triggers apoptosis by binding to its two proapoptotic receptors DR4 and DR5, a process which is negatively regulated by binding of TRAIL to its two decoy rece...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4218

    authors： Sun SY,Yue P,Zhou JY,Wang Y,Choi Kim HR,Lotan R,Wu GS

    更新日期：2001-01-26 00:00:00

  • Protease-sensitive urinary pheromones induce region-specific Fos-expression in rat accessory olfactory bulb.

    abstract：:Vomeronasal organs of female Wistar rats were exposed with sprayed urine preparations of male Wistar rats prior to sacrifice. Exposure to crude urine and ultrafiltrated urine preparation (<5000 Da) induced significant Fos expression, which is correlated with cellular activity, in the mitral/tufted cell layer of the ac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0873

    authors： Tsujikawa K,Kashiwayanagi M

    更新日期：1999-06-24 00:00:00