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Promyelocytic leukemia cell line, HL-60, produces human hepatocyte growth factor.

Abstract：

:The human promyelocytic leukemia cell line, HL-60, stimulated with PMA, produced human HGF-like immunoreactivity (HL-60 HGF), which was detected with human HGF-specific ELISA. The purified HL-60 HGF was indistinguishable from human HGF in the plasma of patients with fulminant hepatic failure by studies of subunit constitution and amino acid composition. The HL-60 HGF mRNA corresponded to 6 kb, which was consistent with previous reported data in rat and human HGF mRNA, was detected in stimulated HL-60, by northern hybridization analysis using human HGF cDNA probe. These findings indicated that HL-60 HGF was identical to, or closely resembled, human plasma HGF. The HL-60 cell is an attractive model for studies of HGF-producing mechanisms, the manner of secretion and the nature of induction signals.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Nishino T,Kaise N,Sindo Y,Nishino N,Nishida T,Yasuda S,Masui Y

doi

10.1016/s0006-291x(05)81421-4

subject

Has Abstract

pub_date

1991-11-27 00:00:00

pages

323-30

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)81421-4

journal_volume

181

pub_type

杂志文章

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