Abstract:
:A polyclonal antiserum raised against purified alpha-agglutinin was made specific for alpha-agglutinin after adsorption with a cells. The adsorbed antiserum identified alpha-agglutinin peptides on Western blots and bound to cell surface alpha-agglutinin, inhibiting the binding of alpha cells to a cells. Using the antibody, we have determined that 1) the surface distribution of alpha-agglutinin on alpha cells is polar, 2) about 5 x 10(4) molecules/cell are constitutively expressed on strain X2180-1B (alpha) cells, and 3) treatment of alpha cells with the sex pheromone a-factor causes an increase in cell surface alpha-agglutinin, consistent with the a-factor induced increase in cell agglutinability.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Wojciechowicz D,Lipke PNdoi
10.1016/0006-291x(89)91557-xsubject
Has Abstractpub_date
1989-05-30 00:00:00pages
46-51issue
1eissn
0006-291Xissn
1090-2104pii
0006-291X(89)91557-Xjournal_volume
161pub_type
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journal_title:Biochemical and biophysical research communications
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更新日期:2001-01-12 00:00:00
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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更新日期:2000-04-29 00:00:00