Identification and distribution of endoplasmic reticulum iPLA2.

abstract：:We established two monoclonal antibodies (mAbs) which specifically recognize human 'functionally inactive' recombinant IL-18, and IL-18 protein polymorphism was examined using human monocytes and macrophages (M phi). In 6 day GM-CSF-treated M phi, an 'inactive' IL-18-recognizing mAb 21 detected the IL-18 proform (24 k...

journal_title：Biochemical and biophysical research communications

authors： Kikkawa S,Matsumoto M,Shida K,Fukumori Y,Toyoshima K,Seya T

更新日期：2001-02-23 00:00:00

abstract：:Systemic administration of 3-nitropropionic acid (3-NP) facilitates the development of select striatal lesions, and some reports provide clues about this pathology. In this study, we investigated the relationship between reduced levels of brain-derived neurotrophic factor (BDNF) in lesioned brain regions and caspase a...

journal_title：Biochemical and biophysical research communications

authors： Cho KJ,Kim GW

更新日期：2015-09-25 00:00:00

abstract：:RNA interference (RNAi) mediated inhibition of virus-specific genes has emerged as a potential therapeutic strategy against virus induced diseases. Human hepatitis B virus (HBV) surface antigen (HBsAg) has proven to be a significant risk factor in HBV induced liver diseases, and an increasing number of mutations in HB...

journal_title：Biochemical and biophysical research communications

authors： Cheng TL,Chang WW,Su IJ,Lai MD,Huang W,Lei HY,Chang WT

更新日期：2005-10-28 00:00:00

abstract：:Growth cone extension is guided by extracellular factors during the brain development but the underlying cellular mechanisms remain largely unclear. Here, we examined the potential function of class-3 semaphorins in cultured cerebellar granule cells. We found neuropilin-2 (NP2), the high-affinity receptor for semaphor...

journal_title：Biochemical and biophysical research communications

authors： Ding S,Luo JH,Yuan XB

更新日期：2007-05-18 00:00:00

abstract：:Guanine nucleotide-binding protein (G protein) coupled receptors (GPCRs) are frequently expressed by a baculovirus expression vector system (BEVS). We recently established a novel BEVS using the bacmid system of Bombyx mori nucleopolyhedrovirus (BmNPV), which is directly applicable for protein expression in silkworms....

journal_title：Biochemical and biophysical research communications

authors： Kajikawa M,Sasaki K,Wakimoto Y,Toyooka M,Motohashi T,Shimojima T,Takeda S,Park EY,Maenaka K

更新日期：2009-07-31 00:00:00

abstract：:Lupeol, present in fruits and medicinal plants, is a biologically active compound that has been shown to have various pharmacological properties in experimental studies. In the present study, we demonstrated the modulatory effect of lupeol on 7,12-dimethylbenz[a]anthracene (DMBA)-induced alterations on cell proliferat...

journal_title：Biochemical and biophysical research communications

authors： Nigam N,Prasad S,George J,Shukla Y

更新日期：2009-04-03 00:00:00

abstract：:The activity of neutral sphingomyelinase (EC 3.1.4.12) in a plasma membrane enriched fraction was found to be increased in dexamethasone treated cells. The elevation of sphingomyelinase activity was blocked by cycloheximide indicating that protein synthesis was required for the steroid action. Ceramidase (EC3.5.1.23) ...

journal_title：Biochemical and biophysical research communications

authors： Ramachandran CK,Murray DK,Nelson DH

更新日期：1990-03-16 00:00:00

abstract：:Corticotropin-releasing factor (CRF) increased intracellular Ca2+ concentration in single astrocytes. The effect in increasing intracellular Ca2+ was not observed in Ca2(+)-free solution. Furthermore, CRF at concentrations more than 10 nM stimulated 45Ca2+ uptake in cultured rat astrocytes. The action was blocked by a...

journal_title：Biochemical and biophysical research communications

authors： Takuma K,Matsuda T,Yoshikawa T,Kitanaka J,Gotoh M,Hayata K,Baba A

更新日期：1994-03-30 00:00:00

abstract：:Human milk Lewis-type (alpha 1,3/4) fucosyltransferase (FT) was separated from the plasma-type by chromatography on bovine IgG glycopep-Sepharose using lactose as the selective eluent and further purified on a column of Sephacryl S-100 HR. The alpha 1,3-FT activity towards 2'-fucosyllactose was found to be associated ...

journal_title：Biochemical and biophysical research communications

authors： Chandrasekaran EV,Rhodes JM,Jain RK,Matta KL

更新日期：1994-01-14 00:00:00

abstract：:Investigations of the coenzyme specificity of N5-methyltetrahydrofolate-homocysteine methyltransferases of diverse biological origin revealed previously unrecognized differences between Escherichia coli methyltransferase and the corresponding enzymes of other species. Cyanocobalamin (CNCbl) actively supports methyltra...

journal_title：Biochemical and biophysical research communications

authors： Beck WS,Thorndike J

更新日期：1984-04-16 00:00:00

abstract：:Ornithine decarboxylase (ODC) was induced in the liver, lung and brain of the mouse injected intraperitoneally with 12-O-tetradecanoylphorbol 13-acetate (TPA), showing maximal enzyme activity four hours after the injection. The increase of ODC activity was due to the enhanced syntheses of mRNA and protein. The inducti...

journal_title：Biochemical and biophysical research communications

authors： Hibasami H,Tsukada T,Maekawa S,Sakurai M,Nakashima K

更新日期：1987-04-29 00:00:00

abstract：:Cysteine proteases and matrix metalloproteinases (MMPs) are important factors in the degradation of organic matrix components of bone. Osteoprotegerin (OPG) is an osteoblast-secreted decoy receptor that inhibits osteoclast differentiation and activation. This study investigated the direct effects of human OPG on cathe...

journal_title：Biochemical and biophysical research communications

authors： Wittrant Y,Couillaud S,Theoleyre S,Dunstan C,Heymann D,Rédini F

更新日期：2002-04-26 00:00:00

abstract：:Hepatitis C virus (HCV) causes chronic hepatitis C (CH-C) and is epidemiologically linked with the occurrence of hepatocellular carcinoma (HCC). To elucidate the comprehensive gene expression profiles of CH-C and HCC, serial analysis of gene expression (SAGE) libraries were made from CH-C and HCC tissues of a patient,...

journal_title：Biochemical and biophysical research communications

authors： Yamashita T,Kaneko S,Hashimoto S,Sato T,Nagai S,Toyoda N,Suzuki T,Kobayashi K,Matsushima K

更新日期：2001-03-30 00:00:00

abstract：:Mycoplasma fermentans has been suspected as one of the causative pathogenic microorganisms of rheumatoid arthritis (RA) however, the pathogenic mechanism is still unclear. We, previously, reported that glycolipid-antigens (GGPL-I and III) are the major antigens of M. fermentans. Monoclonal antibody against the GGPL-II...

journal_title：Biochemical and biophysical research communications

authors： Kawahito Y,Ichinose S,Sano H,Tsubouchi Y,Kohno M,Yoshikawa T,Tokunaga D,Hojo T,Harasawa R,Nakano T,Matsuda K

更新日期：2008-05-02 00:00:00

abstract：:We have established that differing effects of alpha1-adrenergic receptor (AR) subtypes on cell proliferation are due to differential coupling to the Gs/cAMP pathway; thus, both alpha1A- and alpha1B-ARs couple to Gs, while alpha1D-AR does not. To identify the region responsible for this difference in subtype-specific G...

journal_title：Biochemical and biophysical research communications

authors： Shinoura H,Shibata K,Hirasawa A,Tanoue A,Hashimoto K,Tsujimoto G

更新日期：2002-11-22 00:00:00

abstract：:The adhesion of highly activated neutrophils to cerebral microvascular endothelial cells (MVECs) may contribute to disruption and hyperpermeability of the blood-brain barrier (BBB) after cardiac surgery with prolonged cardiopulmonary bypass (CPB). A correlation between CPB duration and neutrophil-mediated BBB damage h...

journal_title：Biochemical and biophysical research communications

authors： Schuller AM,Windolf J,Blaheta R,Cinatl J,Kreuter J,Wimmer-Greinecker G,Moritz A,Scholz M

更新日期：2005-04-08 00:00:00

abstract：:Analysis of a 67 kDa lipid-dependent Ca(2+)-binding protein from avian matrix vesicles revealed amino acid sequences homologous to mammalian annexin VI. PCR methods were used to identify a clone from an avian cDNA library that contained a full length copy of the 67-kDa annexin cDNA. This was restriction mapped, subclo...

journal_title：Biochemical and biophysical research communications

authors： Cao X,Genge BR,Wu LN,Buzzi WR,Showman RM,Wuthier RE

更新日期：1993-12-15 00:00:00

abstract：:We purified the transcription antitermination complex of phi 80 (phi 80-pN complex). This complex had a molecular weight of approx. 42,000, and the subunits of this complex could be separated by SDS polyacrylamide gel electrophoresis. The complex was composed of two different polypeptides, one was the phi 80 N gene pr...

journal_title：Biochemical and biophysical research communications

authors： Kanemoto K,Miyashita T,Matsushiro A

更新日期：1990-05-16 00:00:00

abstract：:Limited proteolysis converts the 39200 molecular weight catalytic domain of rat tyrosine hydroxylase to a monomer with a molecular weight of 37600. The purified monomer is almost fully active, with minor changes in kinetic parameters at pH 7. Mass spectral analysis and N-terminal sequencing of the proteolytically gene...

journal_title：Biochemical and biophysical research communications

authors： Lohse DL,Fitzpatrick PF

更新日期：1993-12-30 00:00:00

abstract：:MicroRNA (miRNA)-mediated gene silencing has recently emerged as a major mechanism of gene expression regulation during development in a variety of species. Little is known, however, about the presence of components of miRNA machinery in mammalian organogenesis. In this study, we report that members of the Argonaute (...

journal_title：Biochemical and biophysical research communications

authors： Lü J,Qian J,Chen F,Tang X,Li C,Cardoso WV

更新日期：2005-08-26 00:00:00

abstract：:Neurons extend neurites with an increased synthesis of phosphatidylcholine (PC) that is not only a membrane component but also a functional regulator with specific fatty acid composition. To analyze the local synthesis of the PC molecular species within neurons, we combined a compartmentalized culture system with matr...

journal_title：Biochemical and biophysical research communications

authors： Sugiyama E,Yao I,Setou M

更新日期：2018-01-01 00:00:00

abstract：:We recently demonstrated that proteoglycan macrophage colony-stimulating factor (PG-M-CSF) binds basic fibroblast growth factor (bFGF) and neutralizes the biological activity of bFGF. In this study, we identified the binding sites of PG-M-CSF for bFGF. We examined the binding of bFGF to overlapping 12-mer peptides wit...

journal_title：Biochemical and biophysical research communications

authors： Suzu S,Kimura F,Matsumoto H,Yamada M,Hashimoto K,Shimamura S,Motoyoshi K

更新日期：1997-01-13 00:00:00

abstract：:During our human heart cDNA sequencing project, we have obtained a novel cDNA clone which is very similar in DNA and amino acid sequences to a rat/mouse cysteine-rich intestinal protein (1). Sequence analysis has shown that this human cysteine-rich heart protein (hCRHP) is a protein of 77 amino acids and possesses a L...

journal_title：Biochemical and biophysical research communications

authors： Tsui SK,Yam NY,Lee CY,Waye MM

更新日期：1994-11-30 00:00:00

abstract：:Intranuclear inclusions have been observed in the brains of patients affected with Huntington's disease (HD). Neuro 2A cells that transiently expressed HD exon 1 bearing 74 glutamine repeats linked to the green fluorescent protein (GFP) and the nuclear localization sequence (NLS) contained aggregates in nuclei. The ag...

journal_title：Biochemical and biophysical research communications

authors： Hazeki N,Tsukamoto T,Yazawa I,Koyama M,Hattori S,Someki I,Iwatsubo T,Nakamura K,Goto J,Kanazawa I

更新日期：2002-06-07 00:00:00

abstract：:Human Kif4A is a member of the Kinesin-4 family of kinesins. Kif4A is thought to be a bona fide chromokinesin because it possesses a motor domain and associates with condensed chromosomes during mitosis. Genetic deletion of Kif4A promotes tumorigenic phenotypes in mouse embryonic cells. Kif4A is critical for mitotic r...

journal_title：Biochemical and biophysical research communications

authors： Wu G,Chen PL

更新日期：2008-08-01 00:00:00

abstract：:Phosphorylation of membranes from murine erythroleukemia cells was performed in the presence and absence of the polar solvent dimethyl sulfoxide. Quantitation of the phosphoamino acid content revealed that DMSO stimulated phosphotyrosine accumulation by three-fold; serine and threonine phosphorylation decreased signif...

journal_title：Biochemical and biophysical research communications

authors： Earp HS,Rubin RA,Austin KS,Dy RC

更新日期：1983-04-29 00:00:00

abstract：:The potential antiatherogenic actions of the angiotensin II receptor antagonist, losartan were investigated in apolipoprotein (apo) E deficient mice, an animal model with severe hypercholesterolemia and extensive atherosclerosis. In these animals accelerated atherosclerosis is associated with increased lipid peroxidat...

journal_title：Biochemical and biophysical research communications

authors： Keidar S,Attias J,Smith J,Breslow JL,Hayek T

更新日期：1997-07-30 00:00:00

abstract：:In a number of cell lines responsive to basic fibroblast growth factor (bFGF), two major tyrosine phosphorylated proteins, of molecular weights around 120kDa and 90kDa, are precipitated along with the tyrosine phosphatase SHP2 from the lysates of stimulated cells. The docker protein Gab-1 represents at least part of t...

journal_title：Biochemical and biophysical research communications

authors： Ong SH,Lim YP,Low BC,Guy GR

更新日期：1997-09-08 00:00:00

abstract：:The cDNA encoding Mn peroxidase isozyme H4 from Phanerochaete chrysosporium was recombined into a baculovirus and heterologously expressed in Sf9 cells. The recombinant Mn peroxidase has the same molecular weight as the native enzyme as determined by SDS-PAGE and cross-reacts with a Mn peroxidase-specific antibody. Th...

journal_title：Biochemical and biophysical research communications

authors： Pease EA,Aust SD,Tien M

更新日期：1991-09-16 00:00:00

abstract：:HTLV-I is implicated with adult T-cell leukemia and certain other clinical disorders. The viral Tax protein is regarded as a key element in HTLV-I pathogenicity due to its ability to activate a wide variety of cellular regulatory factors. As such, Tax may likely activate also latent infection of certain other pathogen...

journal_title：Biochemical and biophysical research communications

authors： Tabakin-Fix Y,Huleihel M,Aboud M

更新日期：2004-06-11 00:00:00