Abstract:
:During our human heart cDNA sequencing project, we have obtained a novel cDNA clone which is very similar in DNA and amino acid sequences to a rat/mouse cysteine-rich intestinal protein (1). Sequence analysis has shown that this human cysteine-rich heart protein (hCRHP) is a protein of 77 amino acids and possesses a LIM motif which is considered to be able to bind zinc. Northern blot analyses have shown that its mRNA level in rat heart is regulated developmentally. We have expressed hCRHP in E. coli using pAED4 as the vector and the cDNA was engineered so that the authentic protein is produced. The protein was partially purified and was shown to be a basic protein.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Tsui SK,Yam NY,Lee CY,Waye MMdoi
10.1006/bbrc.1994.2693subject
Has Abstractpub_date
1994-11-30 00:00:00pages
497-505issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(84)72693-3journal_volume
205pub_type
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