Isolation and characterization of a cDNA that codes for a LIM-containing protein which is developmentally regulated in heart.

Abstract:

:During our human heart cDNA sequencing project, we have obtained a novel cDNA clone which is very similar in DNA and amino acid sequences to a rat/mouse cysteine-rich intestinal protein (1). Sequence analysis has shown that this human cysteine-rich heart protein (hCRHP) is a protein of 77 amino acids and possesses a LIM motif which is considered to be able to bind zinc. Northern blot analyses have shown that its mRNA level in rat heart is regulated developmentally. We have expressed hCRHP in E. coli using pAED4 as the vector and the cDNA was engineered so that the authentic protein is produced. The protein was partially purified and was shown to be a basic protein.

authors

Tsui SK,Yam NY,Lee CY,Waye MM

doi

10.1006/bbrc.1994.2693

subject

Has Abstract

pub_date

1994-11-30 00:00:00

pages

497-505

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(84)72693-3

journal_volume

205

pub_type

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